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Q39548

- KSB_CUCMA

UniProt

Q39548 - KSB_CUCMA

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Protein

Ent-kaur-16-ene synthase, chloroplastic

Gene
N/A
Organism
Cucurbita maxima (Pumpkin) (Winter squash)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of ent-copalyl diphosphate to the gibberellin precursor ent-kaur-16-ene.

Catalytic activityi

Ent-copalyl diphosphate = ent-kaurene + diphosphate.

Cofactori

Binds 3 magnesium ions per subunit.By similarity

Kineticsi

  1. KM=0.35 µM for copalyl diphosphate

pH dependencei

Optimum pH is 6.8-7.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi536 – 5361Magnesium 1By similarity
Metal bindingi536 – 5361Magnesium 2By similarity
Metal bindingi540 – 5401Magnesium 1By similarity
Metal bindingi540 – 5401Magnesium 2By similarity
Metal bindingi680 – 6801Magnesium 3By similarity
Metal bindingi684 – 6841Magnesium 3By similarity
Metal bindingi688 – 6881Magnesium 3By similarity

GO - Molecular functioni

  1. ent-kaurene synthase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. gibberellin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00390.

Names & Taxonomyi

Protein namesi
Recommended name:
Ent-kaur-16-ene synthase, chloroplastic (EC:4.2.3.19)
Alternative name(s):
Ent-kaurene synthase
Ent-kaurene synthase B
Short name:
KSB
OrganismiCucurbita maxima (Pumpkin) (Winter squash)
Taxonomic identifieri3661 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 789Ent-kaur-16-ene synthase, chloroplasticPRO_0000033626
Transit peptidei1 – ?ChloroplastSequence Analysis

Post-translational modificationi

The N-terminus is blocked.

Expressioni

Tissue specificityi

Abundant in most tissues. Present in low amounts in mature cotyledons.

Structurei

3D structure databases

ProteinModelPortaliQ39548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi536 – 5405DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39548-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYLSRPTGVA RFAASSSSSS SASLFPGVDV DTTTKTGALH FEETKERIKK
60 70 80 90 100
LFDKVELSVS AYDTAWVAMV PSPNSLNQPL FPECINWVLD SQHADGSWGL
110 120 130 140 150
LHNDQLLMKA NLLSTLACVL TLKRWNIGHD HMSKALDFIK SNIASATDEN
160 170 180 190 200
QRSPVGFDII FPGMIEYAKD LNLNLPLAPT NVDALVRKKE LELRSCRSNS
210 220 230 240 250
EGGKAYLAYV SEGIGKLQDW DMVMQYQRKN GSLFNSPSTT AAAFMHRNDD
260 270 280 290 300
GCFDYLRSLL QKFDGSVPTI YPLDIYARLH MVDSLQKFGI ARHFKEEIRS
310 320 330 340 350
VLDETYRCWM QGEENIFLDA STCAMAFRML RVEGYDVSSD QLTQFSEDIF
360 370 380 390 400
PNCLGGYLKD FGASLELYKA SQIITHPDES VLENINSWTS RFLKHGLSSD
410 420 430 440 450
SVWSDRTDSV VKQEAVNALE FPYNATLERL ISKRAMESYS GDIVRISKSP
460 470 480 490 500
YACLNFGHQD FLELAVEDFN TLQRIHLKEL EELQRWVVEN KLDELKFFRL
510 520 530 540 550
HLGYCYFAAA ATLTDPELHD ARIAWAQNGV LTTVVDDFYD GGGSEEELDN
560 570 580 590 600
LIELVEKWDP DGEVGYCSKD VEIVFLALHS TVCEIGRRAL VWQGRSVMRN
610 620 630 640 650
VIDGWLALLK VMRKEAEWST NKVVPSMGEY MEQAHVSFAL GPIILPMLFF
660 670 680 690 700
VGPKLSEEMI GSCEYQKLYK LMSTAGRLKN DIRSYDRECK EGKLNILSLW
710 720 730 740 750
MIDGGGNVTK EEAIEAIKGD FERAIRELLG LVLQENTTIP RACKDLFWKL
760 770 780
MSIVNLFYME DDGYTSNRLM NTVKAMFEQP MDLDALLNK
Length:789
Mass (Da):89,362
Last modified:November 1, 1996 - v1
Checksum:i8CC87F2884CCD7F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43904 mRNA. Translation: AAB39482.1.
PIRiT09672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43904 mRNA. Translation: AAB39482.1 .
PIRi T09672.

3D structure databases

ProteinModelPortali Q39548.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00390 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.)."
    Yamaguchi S., Saito T., Abe H., Yamane H., Murofushi N., Kamiya Y.
    Plant J. 10:203-213(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Riesenmelone gelb vernetzt.
    Tissue: Immature seed.
  2. "Purification and properties of ent-kaurene synthase B from immature seeds of pumpkin."
    Saito T., Abe H., Yamane H., Sakurai A., Murofushi N., Takio K., Takahashi N., Kamiya Y.
    Plant Physiol. 109:1239-1245(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 217-229; 370-389; 395-403; 434-448 AND 615-622, CHARACTERIZATION.
    Strain: cv. Riesenmelone gelb vernetzt.
    Tissue: Endosperm.

Entry informationi

Entry nameiKSB_CUCMA
AccessioniPrimary (citable) accession number: Q39548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3