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Protein

Ent-kaur-16-ene synthase, chloroplastic

Gene
N/A
Organism
Cucurbita maxima (Pumpkin) (Winter squash)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of ent-copalyl diphosphate to the gibberellin precursor ent-kaur-16-ene.

Catalytic activityi

Ent-copalyl diphosphate = ent-kaurene + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Kineticsi

  1. KM=0.35 µM for copalyl diphosphate

    pH dependencei

    Optimum pH is 6.8-7.5.

    Pathway: gibberellin biosynthesis

    This protein is involved in the pathway gibberellin biosynthesis, which is part of Plant hormone biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway gibberellin biosynthesis and in Plant hormone biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi536 – 5361Magnesium 1By similarity
    Metal bindingi536 – 5361Magnesium 2By similarity
    Metal bindingi540 – 5401Magnesium 1By similarity
    Metal bindingi540 – 5401Magnesium 2By similarity
    Metal bindingi680 – 6801Magnesium 3By similarity
    Metal bindingi684 – 6841Magnesium 3By similarity
    Metal bindingi688 – 6881Magnesium 3By similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00390.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ent-kaur-16-ene synthase, chloroplastic (EC:4.2.3.19)
    Alternative name(s):
    Ent-kaurene synthase
    Ent-kaurene synthase B
    Short name:
    KSB
    OrganismiCucurbita maxima (Pumpkin) (Winter squash)
    Taxonomic identifieri3661 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 789Ent-kaur-16-ene synthase, chloroplasticPRO_0000033626
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.

    Expressioni

    Tissue specificityi

    Abundant in most tissues. Present in low amounts in mature cotyledons.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39548.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi536 – 5405DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 3 hits.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q39548-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYLSRPTGVA RFAASSSSSS SASLFPGVDV DTTTKTGALH FEETKERIKK
    60 70 80 90 100
    LFDKVELSVS AYDTAWVAMV PSPNSLNQPL FPECINWVLD SQHADGSWGL
    110 120 130 140 150
    LHNDQLLMKA NLLSTLACVL TLKRWNIGHD HMSKALDFIK SNIASATDEN
    160 170 180 190 200
    QRSPVGFDII FPGMIEYAKD LNLNLPLAPT NVDALVRKKE LELRSCRSNS
    210 220 230 240 250
    EGGKAYLAYV SEGIGKLQDW DMVMQYQRKN GSLFNSPSTT AAAFMHRNDD
    260 270 280 290 300
    GCFDYLRSLL QKFDGSVPTI YPLDIYARLH MVDSLQKFGI ARHFKEEIRS
    310 320 330 340 350
    VLDETYRCWM QGEENIFLDA STCAMAFRML RVEGYDVSSD QLTQFSEDIF
    360 370 380 390 400
    PNCLGGYLKD FGASLELYKA SQIITHPDES VLENINSWTS RFLKHGLSSD
    410 420 430 440 450
    SVWSDRTDSV VKQEAVNALE FPYNATLERL ISKRAMESYS GDIVRISKSP
    460 470 480 490 500
    YACLNFGHQD FLELAVEDFN TLQRIHLKEL EELQRWVVEN KLDELKFFRL
    510 520 530 540 550
    HLGYCYFAAA ATLTDPELHD ARIAWAQNGV LTTVVDDFYD GGGSEEELDN
    560 570 580 590 600
    LIELVEKWDP DGEVGYCSKD VEIVFLALHS TVCEIGRRAL VWQGRSVMRN
    610 620 630 640 650
    VIDGWLALLK VMRKEAEWST NKVVPSMGEY MEQAHVSFAL GPIILPMLFF
    660 670 680 690 700
    VGPKLSEEMI GSCEYQKLYK LMSTAGRLKN DIRSYDRECK EGKLNILSLW
    710 720 730 740 750
    MIDGGGNVTK EEAIEAIKGD FERAIRELLG LVLQENTTIP RACKDLFWKL
    760 770 780
    MSIVNLFYME DDGYTSNRLM NTVKAMFEQP MDLDALLNK
    Length:789
    Mass (Da):89,362
    Last modified:November 1, 1996 - v1
    Checksum:i8CC87F2884CCD7F2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U43904 mRNA. Translation: AAB39482.1.
    PIRiT09672.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U43904 mRNA. Translation: AAB39482.1.
    PIRiT09672.

    3D structure databases

    ProteinModelPortaliQ39548.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00390.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 3 hits.
    SSF48576. SSF48576. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.)."
      Yamaguchi S., Saito T., Abe H., Yamane H., Murofushi N., Kamiya Y.
      Plant J. 10:203-213(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Riesenmelone gelb vernetzt.
      Tissue: Immature seed.
    2. "Purification and properties of ent-kaurene synthase B from immature seeds of pumpkin."
      Saito T., Abe H., Yamane H., Sakurai A., Murofushi N., Takio K., Takahashi N., Kamiya Y.
      Plant Physiol. 109:1239-1245(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 217-229; 370-389; 395-403; 434-448 AND 615-622, CHARACTERIZATION.
      Strain: cv. Riesenmelone gelb vernetzt.
      Tissue: Endosperm.

    Entry informationi

    Entry nameiKSB_CUCMA
    AccessioniPrimary (citable) accession number: Q39548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: November 1, 1996
    Last modified: April 1, 2015
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.