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Q39548 (KSB_CUCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ent-kaur-16-ene synthase, chloroplastic

EC=4.2.3.19
Alternative name(s):
Ent-kaurene synthase
Ent-kaurene synthase B
Short name=KSB
OrganismCucurbita maxima (Pumpkin) (Winter squash)
Taxonomic identifier3661 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Protein attributes

Sequence length789 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of ent-copalyl diphosphate to the gibberellin precursor ent-kaur-16-ene.

Catalytic activity

Ent-copalyl diphosphate = ent-kaurene + diphosphate.

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Plant hormone biosynthesis; gibberellin biosynthesis.

Subcellular location

Plastidchloroplast.

Tissue specificity

Abundant in most tissues. Present in low amounts in mature cotyledons.

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the terpene synthase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.35 µM for copalyl diphosphate

pH dependence:

Optimum pH is 6.8-7.5.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandMagnesium
Metal-binding
   Molecular functionLyase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processgibberellin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionent-kaurene synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 789Ent-kaur-16-ene synthase, chloroplasticPRO_0000033626

Regions

Motif536 – 5405DDXXD motif

Sites

Metal binding5361Magnesium 1 By similarity
Metal binding5361Magnesium 2 By similarity
Metal binding5401Magnesium 1 By similarity
Metal binding5401Magnesium 2 By similarity
Metal binding6801Magnesium 3 By similarity
Metal binding6841Magnesium 3 By similarity
Metal binding6881Magnesium 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q39548 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8CC87F2884CCD7F2

FASTA78989,362
        10         20         30         40         50         60 
MYLSRPTGVA RFAASSSSSS SASLFPGVDV DTTTKTGALH FEETKERIKK LFDKVELSVS 

        70         80         90        100        110        120 
AYDTAWVAMV PSPNSLNQPL FPECINWVLD SQHADGSWGL LHNDQLLMKA NLLSTLACVL 

       130        140        150        160        170        180 
TLKRWNIGHD HMSKALDFIK SNIASATDEN QRSPVGFDII FPGMIEYAKD LNLNLPLAPT 

       190        200        210        220        230        240 
NVDALVRKKE LELRSCRSNS EGGKAYLAYV SEGIGKLQDW DMVMQYQRKN GSLFNSPSTT 

       250        260        270        280        290        300 
AAAFMHRNDD GCFDYLRSLL QKFDGSVPTI YPLDIYARLH MVDSLQKFGI ARHFKEEIRS 

       310        320        330        340        350        360 
VLDETYRCWM QGEENIFLDA STCAMAFRML RVEGYDVSSD QLTQFSEDIF PNCLGGYLKD 

       370        380        390        400        410        420 
FGASLELYKA SQIITHPDES VLENINSWTS RFLKHGLSSD SVWSDRTDSV VKQEAVNALE 

       430        440        450        460        470        480 
FPYNATLERL ISKRAMESYS GDIVRISKSP YACLNFGHQD FLELAVEDFN TLQRIHLKEL 

       490        500        510        520        530        540 
EELQRWVVEN KLDELKFFRL HLGYCYFAAA ATLTDPELHD ARIAWAQNGV LTTVVDDFYD 

       550        560        570        580        590        600 
GGGSEEELDN LIELVEKWDP DGEVGYCSKD VEIVFLALHS TVCEIGRRAL VWQGRSVMRN 

       610        620        630        640        650        660 
VIDGWLALLK VMRKEAEWST NKVVPSMGEY MEQAHVSFAL GPIILPMLFF VGPKLSEEMI 

       670        680        690        700        710        720 
GSCEYQKLYK LMSTAGRLKN DIRSYDRECK EGKLNILSLW MIDGGGNVTK EEAIEAIKGD 

       730        740        750        760        770        780 
FERAIRELLG LVLQENTTIP RACKDLFWKL MSIVNLFYME DDGYTSNRLM NTVKAMFEQP 


MDLDALLNK 

« Hide

References

[1]"Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.)."
Yamaguchi S., Saito T., Abe H., Yamane H., Murofushi N., Kamiya Y.
Plant J. 10:203-213(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Riesenmelone gelb vernetzt.
Tissue: Immature seed.
[2]"Purification and properties of ent-kaurene synthase B from immature seeds of pumpkin."
Saito T., Abe H., Yamane H., Sakurai A., Murofushi N., Takio K., Takahashi N., Kamiya Y.
Plant Physiol. 109:1239-1245(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 217-229; 370-389; 395-403; 434-448 AND 615-622, CHARACTERIZATION.
Strain: cv. Riesenmelone gelb vernetzt.
Tissue: Endosperm.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43904 mRNA. Translation: AAB39482.1.
PIRT09672.

3D structure databases

ProteinModelPortalQ39548.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00390.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKSB_CUCMA
AccessionPrimary (citable) accession number: Q39548
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways