Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q39548

- KSB_CUCMA

UniProt

Q39548 - KSB_CUCMA

Protein

Ent-kaur-16-ene synthase, chloroplastic

Gene
N/A
Organism
Cucurbita maxima (Pumpkin) (Winter squash)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of ent-copalyl diphosphate to the gibberellin precursor ent-kaur-16-ene.

    Catalytic activityi

    Ent-copalyl diphosphate = ent-kaurene + diphosphate.

    Cofactori

    Binds 3 magnesium ions per subunit.By similarity

    Kineticsi

    1. KM=0.35 µM for copalyl diphosphate

    pH dependencei

    Optimum pH is 6.8-7.5.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi536 – 5361Magnesium 1By similarity
    Metal bindingi536 – 5361Magnesium 2By similarity
    Metal bindingi540 – 5401Magnesium 1By similarity
    Metal bindingi540 – 5401Magnesium 2By similarity
    Metal bindingi680 – 6801Magnesium 3By similarity
    Metal bindingi684 – 6841Magnesium 3By similarity
    Metal bindingi688 – 6881Magnesium 3By similarity

    GO - Molecular functioni

    1. ent-kaurene synthase activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. gibberellin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00390.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ent-kaur-16-ene synthase, chloroplastic (EC:4.2.3.19)
    Alternative name(s):
    Ent-kaurene synthase
    Ent-kaurene synthase B
    Short name:
    KSB
    OrganismiCucurbita maxima (Pumpkin) (Winter squash)
    Taxonomic identifieri3661 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 789Ent-kaur-16-ene synthase, chloroplasticPRO_0000033626
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.

    Expressioni

    Tissue specificityi

    Abundant in most tissues. Present in low amounts in mature cotyledons.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39548.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi536 – 5405DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 3 hits.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q39548-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYLSRPTGVA RFAASSSSSS SASLFPGVDV DTTTKTGALH FEETKERIKK    50
    LFDKVELSVS AYDTAWVAMV PSPNSLNQPL FPECINWVLD SQHADGSWGL 100
    LHNDQLLMKA NLLSTLACVL TLKRWNIGHD HMSKALDFIK SNIASATDEN 150
    QRSPVGFDII FPGMIEYAKD LNLNLPLAPT NVDALVRKKE LELRSCRSNS 200
    EGGKAYLAYV SEGIGKLQDW DMVMQYQRKN GSLFNSPSTT AAAFMHRNDD 250
    GCFDYLRSLL QKFDGSVPTI YPLDIYARLH MVDSLQKFGI ARHFKEEIRS 300
    VLDETYRCWM QGEENIFLDA STCAMAFRML RVEGYDVSSD QLTQFSEDIF 350
    PNCLGGYLKD FGASLELYKA SQIITHPDES VLENINSWTS RFLKHGLSSD 400
    SVWSDRTDSV VKQEAVNALE FPYNATLERL ISKRAMESYS GDIVRISKSP 450
    YACLNFGHQD FLELAVEDFN TLQRIHLKEL EELQRWVVEN KLDELKFFRL 500
    HLGYCYFAAA ATLTDPELHD ARIAWAQNGV LTTVVDDFYD GGGSEEELDN 550
    LIELVEKWDP DGEVGYCSKD VEIVFLALHS TVCEIGRRAL VWQGRSVMRN 600
    VIDGWLALLK VMRKEAEWST NKVVPSMGEY MEQAHVSFAL GPIILPMLFF 650
    VGPKLSEEMI GSCEYQKLYK LMSTAGRLKN DIRSYDRECK EGKLNILSLW 700
    MIDGGGNVTK EEAIEAIKGD FERAIRELLG LVLQENTTIP RACKDLFWKL 750
    MSIVNLFYME DDGYTSNRLM NTVKAMFEQP MDLDALLNK 789
    Length:789
    Mass (Da):89,362
    Last modified:November 1, 1996 - v1
    Checksum:i8CC87F2884CCD7F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43904 mRNA. Translation: AAB39482.1.
    PIRiT09672.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43904 mRNA. Translation: AAB39482.1 .
    PIRi T09672.

    3D structure databases

    ProteinModelPortali Q39548.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00390 .

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProi IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 3 hits.
    SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.)."
      Yamaguchi S., Saito T., Abe H., Yamane H., Murofushi N., Kamiya Y.
      Plant J. 10:203-213(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Riesenmelone gelb vernetzt.
      Tissue: Immature seed.
    2. "Purification and properties of ent-kaurene synthase B from immature seeds of pumpkin."
      Saito T., Abe H., Yamane H., Sakurai A., Murofushi N., Takio K., Takahashi N., Kamiya Y.
      Plant Physiol. 109:1239-1245(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 217-229; 370-389; 395-403; 434-448 AND 615-622, CHARACTERIZATION.
      Strain: cv. Riesenmelone gelb vernetzt.
      Tissue: Endosperm.

    Entry informationi

    Entry nameiKSB_CUCMA
    AccessioniPrimary (citable) accession number: Q39548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3