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Protein

Cucumisin

Gene
N/A
Organism
Cucumis melo (Muskmelon)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of proteins with broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei140 – 1401Charge relay systemPROSITE-ProRule annotation
Active sitei204 – 2041Charge relay systemPROSITE-ProRule annotation
Active sitei525 – 5251Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS08.092.

Names & Taxonomyi

Protein namesi
Recommended name:
Cucumisin (EC:3.4.21.25)
Alternative name(s):
Allergen: Cuc m 1
OrganismiCucumis melo (Muskmelon)
Taxonomic identifieri3656 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei255. Cuc m 1.
3229. Cuc m 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 11088Activation peptidePRO_0000027003Add
BLAST
Chaini111 – 615505CucumisinPRO_0000027004Add
BLAST
Propeptidei616 – 731116PRO_0000027005Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi466 – 4661N-linked (GlcNAc...)1 Publication
Glycosylationi652 – 6521N-linked (GlcNAc...)1 Publication

Post-translational modificationi

The C-terminal propeptide is autocleaved.

Keywords - PTMi

Autocatalytic cleavage, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Specifically expressed in fruits. Expressed in sarcocarp (at protein level).2 Publications

Structurei

Secondary structure

1
731
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 409Combined sources
Helixi66 – 683Combined sources
Beta strandi69 – 735Combined sources
Beta strandi75 – 8410Combined sources
Helixi86 – 949Combined sources
Beta strandi95 – 973Combined sources
Beta strandi100 – 1034Combined sources
Beta strandi106 – 1094Combined sources
Helixi114 – 1174Combined sources
Helixi128 – 1314Combined sources
Beta strandi135 – 1417Combined sources
Helixi148 – 1503Combined sources
Turni169 – 1713Combined sources
Beta strandi176 – 1838Combined sources
Beta strandi187 – 1893Combined sources
Beta strandi196 – 1994Combined sources
Beta strandi201 – 2033Combined sources
Helixi204 – 21310Combined sources
Beta strandi217 – 2226Combined sources
Beta strandi225 – 2306Combined sources
Beta strandi237 – 2437Combined sources
Beta strandi249 – 2513Combined sources
Helixi252 – 26514Combined sources
Beta strandi268 – 2725Combined sources
Helixi282 – 2843Combined sources
Helixi286 – 29510Combined sources
Turni296 – 2983Combined sources
Beta strandi300 – 3045Combined sources
Beta strandi310 – 3134Combined sources
Beta strandi322 – 3287Combined sources
Beta strandi331 – 34010Combined sources
Beta strandi345 – 3495Combined sources
Beta strandi357 – 3637Combined sources
Helixi364 – 3663Combined sources
Helixi374 – 3774Combined sources
Turni378 – 3803Combined sources
Turni387 – 3893Combined sources
Turni390 – 3923Combined sources
Beta strandi393 – 3964Combined sources
Helixi403 – 4097Combined sources
Beta strandi414 – 4196Combined sources
Beta strandi430 – 4367Combined sources
Helixi438 – 44811Combined sources
Beta strandi451 – 4533Combined sources
Beta strandi455 – 4584Combined sources
Beta strandi462 – 4654Combined sources
Beta strandi482 – 4843Combined sources
Beta strandi492 – 4954Combined sources
Beta strandi497 – 5026Combined sources
Beta strandi505 – 5073Combined sources
Beta strandi517 – 5215Combined sources
Helixi524 – 54118Combined sources
Helixi547 – 55610Combined sources
Turni563 – 5653Combined sources
Turni567 – 5693Combined sources
Helixi570 – 5734Combined sources
Helixi580 – 5834Combined sources
Beta strandi587 – 5893Combined sources
Helixi594 – 5974Combined sources
Helixi629 – 6313Combined sources
Beta strandi635 – 6406Combined sources
Beta strandi647 – 65711Combined sources
Beta strandi659 – 6613Combined sources
Beta strandi663 – 6708Combined sources
Beta strandi675 – 68612Combined sources
Beta strandi692 – 70110Combined sources
Beta strandi704 – 71815Combined sources
Beta strandi720 – 7289Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VTAX-ray2.75A/B111-731[»]
4YN3X-ray1.95A111-731[»]
B23-110[»]
ProteinModelPortaliQ39547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini135 – 584450Peptidase S8Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi157 – 1604Poly-Pro

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.200. 2 hits.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSLIFKLF FFSLFFSNRL ASRLDSDDDG KNIYIVYMGR KLEDPDSAHL
60 70 80 90 100
HHRAMLEQVV GSTFAPESVL HTYKRSFNGF AVKLTEEEAE KIASMEGVVS
110 120 130 140 150
VFLNEMNELH TTRSWDFLGF PLTVPRRSQV ESNIVVGVLD TGIWPESPSF
160 170 180 190 200
DDEGFSPPPP KWKGTCETSN NFRCNRKIIG ARSYHIGRPI SPGDVNGPRD
210 220 230 240 250
TNGHGTHTAS TAAGGLVSQA NLYGLGLGTA RGGVPLARIA AYKVCWNDGC
260 270 280 290 300
SDTDILAAYD DAIADGVDII SLSVGGANPR HYFVDAIAIG SFHAVERGIL
310 320 330 340 350
TSNSAGNGGP NFFTTASLSP WLLSVAASTM DRKFVTQVQI GNGQSFQGVS
360 370 380 390 400
INTFDNQYYP LVSGRDIPNT GFDKSTSRFC TDKSVNPNLL KGKIVVCEAS
410 420 430 440 450
FGPHEFFKSL DGAAGVLMTS NTRDYADSYP LPSSVLDPND LLATLRYIYS
460 470 480 490 500
IRSPGATIFK STTILNASAP VVVSFSSRGP NRATKDVIKP DISGPGVEIL
510 520 530 540 550
AAWPSVAPVG GIRRNTLFNI ISGTSMSCPH ITGIATYVKT YNPTWSPAAI
560 570 580 590 600
KSALMTTASP MNARFNPQAE FAYGSGHVNP LKAVRPGLVY DANESDYVKF
610 620 630 640 650
LCGQGYNTQA VRRITGDYSA CTSGNTGRVW DLNYPSFGLS VSPSQTFNQY
660 670 680 690 700
FNRTLTSVAP QASTYRAMIS APQGLTISVN PNVLSFNGLG DRKSFTLTVR
710 720 730
GSIKGFVVSA SLVWSDGVHY VRSPITITSL V
Length:731
Mass (Da):78,820
Last modified:November 1, 1996 - v1
Checksum:i93270A9433A366ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181L → K AA sequence (PubMed:7806492).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32206 mRNA. Translation: BAA06905.1.
AY055805 Genomic DNA. Translation: AAL25196.1.
PIRiA55800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32206 mRNA. Translation: BAA06905.1.
AY055805 Genomic DNA. Translation: AAL25196.1.
PIRiA55800.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VTAX-ray2.75A/B111-731[»]
4YN3X-ray1.95A111-731[»]
B23-110[»]
ProteinModelPortaliQ39547.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei255. Cuc m 1.
3229. Cuc m 1.0101.
MEROPSiS08.092.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.200. 2 hits.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUCM1_CUCME
AccessioniPrimary (citable) accession number: Q39547
Secondary accession number(s): Q940D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.