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Protein

Cucumisin

Gene
N/A
Organism
Cucumis melo (Muskmelon)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of proteins with broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei140Charge relay systemPROSITE-ProRule annotation1
Active sitei204Charge relay systemPROSITE-ProRule annotation1
Active sitei525Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS08.092.

Names & Taxonomyi

Protein namesi
Recommended name:
Cucumisin (EC:3.4.21.25)
Alternative name(s):
Allergen: Cuc m 1
OrganismiCucumis melo (Muskmelon)
Taxonomic identifieri3656 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei255. Cuc m 1.
3229. Cuc m 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000002700323 – 110Activation peptideAdd BLAST88
ChainiPRO_0000027004111 – 615CucumisinAdd BLAST505
PropeptideiPRO_0000027005616 – 731Add BLAST116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi466N-linked (GlcNAc...)1 Publication1
Glycosylationi652N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

The C-terminal propeptide is autocleaved.

Keywords - PTMi

Autocatalytic cleavage, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Specifically expressed in fruits. Expressed in sarcocarp (at protein level).2 Publications

Structurei

Secondary structure

1731
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 40Combined sources9
Helixi66 – 68Combined sources3
Beta strandi69 – 73Combined sources5
Beta strandi75 – 84Combined sources10
Helixi86 – 94Combined sources9
Beta strandi95 – 97Combined sources3
Beta strandi100 – 103Combined sources4
Beta strandi106 – 109Combined sources4
Helixi114 – 117Combined sources4
Helixi128 – 131Combined sources4
Beta strandi135 – 141Combined sources7
Helixi148 – 150Combined sources3
Turni169 – 171Combined sources3
Beta strandi176 – 183Combined sources8
Beta strandi187 – 189Combined sources3
Beta strandi196 – 199Combined sources4
Beta strandi201 – 203Combined sources3
Helixi204 – 213Combined sources10
Beta strandi217 – 222Combined sources6
Beta strandi225 – 230Combined sources6
Beta strandi237 – 243Combined sources7
Beta strandi249 – 251Combined sources3
Helixi252 – 265Combined sources14
Beta strandi268 – 272Combined sources5
Helixi282 – 284Combined sources3
Helixi286 – 295Combined sources10
Turni296 – 298Combined sources3
Beta strandi300 – 304Combined sources5
Beta strandi310 – 313Combined sources4
Beta strandi322 – 328Combined sources7
Beta strandi331 – 340Combined sources10
Beta strandi345 – 349Combined sources5
Beta strandi357 – 363Combined sources7
Helixi364 – 366Combined sources3
Helixi374 – 377Combined sources4
Turni378 – 380Combined sources3
Turni387 – 389Combined sources3
Turni390 – 392Combined sources3
Beta strandi393 – 396Combined sources4
Helixi403 – 409Combined sources7
Beta strandi414 – 419Combined sources6
Beta strandi430 – 436Combined sources7
Helixi438 – 448Combined sources11
Beta strandi451 – 453Combined sources3
Beta strandi455 – 458Combined sources4
Beta strandi462 – 465Combined sources4
Beta strandi482 – 484Combined sources3
Beta strandi492 – 495Combined sources4
Beta strandi497 – 502Combined sources6
Beta strandi505 – 507Combined sources3
Beta strandi517 – 521Combined sources5
Helixi524 – 541Combined sources18
Helixi547 – 556Combined sources10
Turni563 – 565Combined sources3
Turni567 – 569Combined sources3
Helixi570 – 573Combined sources4
Helixi580 – 583Combined sources4
Beta strandi587 – 589Combined sources3
Helixi594 – 597Combined sources4
Helixi629 – 631Combined sources3
Beta strandi635 – 640Combined sources6
Beta strandi647 – 657Combined sources11
Beta strandi659 – 661Combined sources3
Beta strandi663 – 670Combined sources8
Beta strandi675 – 686Combined sources12
Beta strandi692 – 701Combined sources10
Beta strandi704 – 718Combined sources15
Beta strandi720 – 728Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VTAX-ray2.75A/B111-731[»]
4YN3X-ray1.95A111-731[»]
B23-110[»]
ProteinModelPortaliQ39547.
SMRiQ39547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 584Peptidase S8Add BLAST450

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi157 – 160Poly-Pro4

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 2 hits.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSLIFKLF FFSLFFSNRL ASRLDSDDDG KNIYIVYMGR KLEDPDSAHL
60 70 80 90 100
HHRAMLEQVV GSTFAPESVL HTYKRSFNGF AVKLTEEEAE KIASMEGVVS
110 120 130 140 150
VFLNEMNELH TTRSWDFLGF PLTVPRRSQV ESNIVVGVLD TGIWPESPSF
160 170 180 190 200
DDEGFSPPPP KWKGTCETSN NFRCNRKIIG ARSYHIGRPI SPGDVNGPRD
210 220 230 240 250
TNGHGTHTAS TAAGGLVSQA NLYGLGLGTA RGGVPLARIA AYKVCWNDGC
260 270 280 290 300
SDTDILAAYD DAIADGVDII SLSVGGANPR HYFVDAIAIG SFHAVERGIL
310 320 330 340 350
TSNSAGNGGP NFFTTASLSP WLLSVAASTM DRKFVTQVQI GNGQSFQGVS
360 370 380 390 400
INTFDNQYYP LVSGRDIPNT GFDKSTSRFC TDKSVNPNLL KGKIVVCEAS
410 420 430 440 450
FGPHEFFKSL DGAAGVLMTS NTRDYADSYP LPSSVLDPND LLATLRYIYS
460 470 480 490 500
IRSPGATIFK STTILNASAP VVVSFSSRGP NRATKDVIKP DISGPGVEIL
510 520 530 540 550
AAWPSVAPVG GIRRNTLFNI ISGTSMSCPH ITGIATYVKT YNPTWSPAAI
560 570 580 590 600
KSALMTTASP MNARFNPQAE FAYGSGHVNP LKAVRPGLVY DANESDYVKF
610 620 630 640 650
LCGQGYNTQA VRRITGDYSA CTSGNTGRVW DLNYPSFGLS VSPSQTFNQY
660 670 680 690 700
FNRTLTSVAP QASTYRAMIS APQGLTISVN PNVLSFNGLG DRKSFTLTVR
710 720 730
GSIKGFVVSA SLVWSDGVHY VRSPITITSL V
Length:731
Mass (Da):78,820
Last modified:November 1, 1996 - v1
Checksum:i93270A9433A366ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti118L → K AA sequence (PubMed:7806492).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32206 mRNA. Translation: BAA06905.1.
AY055805 Genomic DNA. Translation: AAL25196.1.
PIRiA55800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32206 mRNA. Translation: BAA06905.1.
AY055805 Genomic DNA. Translation: AAL25196.1.
PIRiA55800.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VTAX-ray2.75A/B111-731[»]
4YN3X-ray1.95A111-731[»]
B23-110[»]
ProteinModelPortaliQ39547.
SMRiQ39547.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei255. Cuc m 1.
3229. Cuc m 1.0101.
MEROPSiS08.092.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 2 hits.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUCM1_CUCME
AccessioniPrimary (citable) accession number: Q39547
Secondary accession number(s): Q940D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.