Reviewed, UniProtKB/Swiss-Prot Q39547 (CUCM1_CUCME)

Names and origin

Protein names	Recommended name:     Cucumisin     EC=3.4.21.25 Alternative name(s):     Allergen=Cuc m 1
Organism	Cucumis melo (Muskmelon)
Taxonomic identifier	3656 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of proteins with broad specificity.
Subcellular location	Secreted.
Tissue specificity	Specifically expressed in fruits. Expressed in sarcocarp (at protein level). Ref.1 Ref.2
Post-translational modification	The C-terminal propeptide is autocleaved. Ref.1
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Ref.3
Sequence similarities	Belongs to the peptidase S8 family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Autocatalytic cleavage Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of catalytic activity Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 110	88	Activation peptide Ref.1 Ref.3		PRO_0000027003
	Chain	111 – 615	505	Cucumisin		PRO_0000027004
	Propeptide	616 – 731	116			PRO_0000027005
Regions
	Compositional bias	157 – 160	4	Poly-Pro
Sites
	Active site	140	1	Charge relay system By similarity
	Active site	204	1	Charge relay system By similarity
	Active site	525	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	466	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	652	1	N-linked (GlcNAc...) Ref.4
Experimental info
	Sequence conflict	118	1	L → K AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q39547-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 93270A9433A366ED	FASTA	731	78,820
	10 20 30 40 50 60 MSSSLIFKLF FFSLFFSNRL ASRLDSDDDG KNIYIVYMGR KLEDPDSAHL HHRAMLEQVV 70 80 90 100 110 120 GSTFAPESVL HTYKRSFNGF AVKLTEEEAE KIASMEGVVS VFLNEMNELH TTRSWDFLGF 130 140 150 160 170 180 PLTVPRRSQV ESNIVVGVLD TGIWPESPSF DDEGFSPPPP KWKGTCETSN NFRCNRKIIG 190 200 210 220 230 240 ARSYHIGRPI SPGDVNGPRD TNGHGTHTAS TAAGGLVSQA NLYGLGLGTA RGGVPLARIA 250 260 270 280 290 300 AYKVCWNDGC SDTDILAAYD DAIADGVDII SLSVGGANPR HYFVDAIAIG SFHAVERGIL 310 320 330 340 350 360 TSNSAGNGGP NFFTTASLSP WLLSVAASTM DRKFVTQVQI GNGQSFQGVS INTFDNQYYP 370 380 390 400 410 420 LVSGRDIPNT GFDKSTSRFC TDKSVNPNLL KGKIVVCEAS FGPHEFFKSL DGAAGVLMTS 430 440 450 460 470 480 NTRDYADSYP LPSSVLDPND LLATLRYIYS IRSPGATIFK STTILNASAP VVVSFSSRGP 490 500 510 520 530 540 NRATKDVIKP DISGPGVEIL AAWPSVAPVG GIRRNTLFNI ISGTSMSCPH ITGIATYVKT 550 560 570 580 590 600 YNPTWSPAAI KSALMTTASP MNARFNPQAE FAYGSGHVNP LKAVRPGLVY DANESDYVKF 610 620 630 640 650 660 LCGQGYNTQA VRRITGDYSA CTSGNTGRVW DLNYPSFGLS VSPSQTFNQY FNRTLTSVAP 670 680 690 700 710 720 QASTYRAMIS APQGLTISVN PNVLSFNGLG DRKSFTLTVR GSIKGFVVSA SLVWSDGVHY 730 VRSPITITSL V

References

[1]	"Cucumisin, a serine protease from melon fruits, shares structural homology with subtilisin and is generated from a large precursor." Yamagata H., Masuzawa T., Nagaoka Y., Ohnishi T., Iwasaki T. J. Biol. Chem. 269:32725-32731(1994) [PubMed: 7806492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 111-123 AND 616-622, TISSUE SPECIFICITY, PROTEOLYTIC PROCESSING. Strain: cv. Reticulatus / Teresa. Tissue: Fruit.
[2]	"TGTCACA motif is a novel cis-regulatory enhancer element involved in fruit-specific expression of the cucumisin gene." Yamagata H., Yonesu K., Hirata A., Aizono Y. J. Biol. Chem. 277:11582-11590(2002) [PubMed: 11782472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-181, TISSUE SPECIFICITY. Strain: cv. Reticulatus / Teresa. Tissue: Fruit.
[3]	"Identification of Cucumisin (Cuc m 1), a subtilisin-like endopeptidase, as the major allergen of melon fruit." Cuesta-Herranz J., Pastor C., Figueredo E., Vidarte L., De las Heras M., Duran C., Fernandez-Caldas E., de Miguel J., Vivanco F. Clin. Exp. Allergy 33:827-833(2003) [PubMed: 12801320] [Abstract] Cited for: PROTEIN SEQUENCE OF 111-118, ALLERGENICITY.
[4]	"Amino acid sequences of glycopeptides from Cucumisin." Kaneda M., Kamikubo Y., Tominaga N. Agric. Biol. Chem. 50:2413-2414(1986) [Agricola: IND87003744] Cited for: PROTEIN SEQUENCE OF 466-468 AND 652-654, GLYCOSYLATION AT ASN-466 AND ASN-652.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D32206 mRNA. Translation: BAA06905.1. AY055805 Genomic DNA. Translation: AAL25196.1.
PIR	A55800.
3D structure databases
HSSP	HSSP built from PDB template 1ST3 based on UniProtKB P29599.
ModBase	Search...
Protein family/group databases
MEROPS	S08.092.
Enzyme and pathway databases
BRENDA	3.4.21.25. 3933.
Family and domain databases
InterPro	IPR003137. PA. IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR010259. Prot_inh_S8A. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF05922. Inhibitor_I9. 1 hit. PF02225. PA. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
PROSITE	PS00136. SUBTILASE_ASP. False negative. PS00137. SUBTILASE_HIS. False negative. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CUCM1_CUCME

Accession

Primary (citable) accession number: Q39547
Secondary accession number(s): Q940D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)