Q394V3 (ILVD1_BURS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 1
Short name=DAD 1
EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	Bcep18194_B1899

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000225379
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	201	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q394V3 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 73C08F7AD3CA3B01	FASTA	619	66,482
	10 20 30 40 50 60 MPTYRSKTST AGRNMAGARS LWRATGMKDD DFSKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEAAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRDII ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPV IFVSGGPMEA GKTRLANPVT KAIEVKKLDL VDAMVIAVDP 190 200 210 220 230 240 SYSDAEVAEV ERSACPTCGS CSGMFTANSM NCLTEALGLS LPGNGTVVAT HADREQLFKR 250 260 270 280 290 300 AGRRIVELTR QHYEQDDERV LPRSVGFKAF ENAMTLDIAM GGSTNTILHL LAIAQEAGID 310 320 330 340 350 360 FTMKDIDRLS RVVPQLCKVA PNTNKYHIED VHRAGGIMAI LGELDRAGKL HTDVPTVHTP 370 380 390 400 410 420 SLKDALDQWD IVRTQDDAVR TFYQAGPAGV PTQVAFSQNT RWPSLDLDRA EGCIRSYEHA 430 440 450 460 470 480 FSKEGGLAVL TGNIALDGCV VKTAGVDESI LVFEGTAHVT ESQDEAVENI LNDKVKAGDV 490 500 510 520 530 540 VIVRYEGPKG GPGMQEMLYP TSYIKSKGLG KACALLTDGR FSGGTSGLSI GHCSPEAAAG 550 560 570 580 590 600 GAIGLVRDGD KIRIDIPNRT INVLVSDEEL ARRREEQNAK GWKPAQPRPR KVSAALKAYA 610 KLVMSADKGA VRDLSLLDD

References

[1]

"Complete sequence of chromosome 2 of Burkholderia sp. 383." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000152 Genomic DNA. Translation: ABB12013.1.
RefSeq	YP_372657.1. NC_007511.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q394V3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3753664.
GenomeReviews	Gene locus Bcep18194_B1899 in contig CP000152_GR.
KEGG	bur:Bcep18194_B1899.
NMPDR	fig|269483.3.peg.1907.
PATRIC	19294657. VBIBurSp120713_6690.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_B1899-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_BURS3

Accession

Primary (citable) accession number: Q394V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program