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Protein

Alanine racemase

Gene

Bcep18194_B1965

Organism
Burkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: D-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase (Bcep18194_A5348), Alanine racemase (Bcep18194_B1965), Alanine racemase (VL00_18935), Alanine racemase (VL00_21010)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei35 – 351Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei130 – 1301SubstrateUniRule annotation
Active sitei252 – 2521Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei300 – 3001Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotationImported

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBSP269483:GHLS-5384-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Ordered Locus Names:Bcep18194_B1965Imported
OrganismiBurkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)Imported
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002705 Componenti: Chromosome 2

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351N6-(pyridoxal phosphate)lysineUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ394N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini231 – 355125Ala_racemase_CInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000031446.
KOiK01775.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q394N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRPIVAHIR PDAVRHNLDF IRRTAAQSRV WGVVKADAYG HGIERIYPGL
60 70 80 90 100
AAADGIALLD LDEAVRVREL GWDKPVLLLE GIFEPADVEL ADRHRLTVAV
110 120 130 140 150
HCDEQLDLLI AARPQQPIDI QLKMNSGMNR LGYRPAAFRA AWERAASAPS
160 170 180 190 200
IGKITLMMHF ANADEGEVDW QLDQFDATTA GIPGERSTSN SAAVLWHPRA
210 220 230 240 250
HRDWVRPGTI LYGASPTGAA RDIADTPLMA AMTLTSKIIG VQTLSPEETV
260 270 280 290 300
GYGRRFTADR PMRIGVVACG YADGYPRHAP TGTPIAVDGV MTRVVGRVSM
310 320 330 340 350
DMLTVDLTPC PNAGIGSSVE LWGDQVKVDD VAEASGTIGY ELMCALARRV
360 370
PVVIVPPGAS SEQPTLRTGS YGR
Length:373
Mass (Da):40,326
Last modified:November 22, 2005 - v1
Checksum:i96D98CB104437B8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000152 Genomic DNA. Translation: ABB12078.1.
RefSeqiWP_011355562.1. NC_007511.1.

Genome annotation databases

EnsemblBacteriaiABB12078; ABB12078; Bcep18194_B1965.
KEGGibur:Bcep18194_B1965.
PATRICi19294801. VBIBurSp120713_6762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000152 Genomic DNA. Translation: ABB12078.1.
RefSeqiWP_011355562.1. NC_007511.1.

3D structure databases

ProteinModelPortaliQ394N8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB12078; ABB12078; Bcep18194_B1965.
KEGGibur:Bcep18194_B1965.
PATRICi19294801. VBIBurSp120713_6762.

Phylogenomic databases

HOGENOMiHOG000031446.
KOiK01775.
OrthoDBiEOG6PP9NJ.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciBSP269483:GHLS-5384-MONOMER.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 2 of Burkholderia sp. 383."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383Imported.

Entry informationi

Entry nameiQ394N8_BURL3
AccessioniPrimary (citable) accession number: Q394N8
Entry historyi
Integrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.