Reviewed, UniProtKB/Swiss-Prot Q394I6 (CHEB1_BURS3)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Bcep18194_B2017

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225449
Regions
	Domain	2 – 120	119	Response regulatory
	Domain	145 – 334	190	CheB-type methylesterase
Sites
	Active site	157	1	By similarity
	Active site	184	1	By similarity
	Active site	277	1	By similarity
Amino acid modifications
	Modified residue	53	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q394I6-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 75A9209A9F46423E	FASTA	334	34,802
	10 20 30 40 50 60 MNIGIVNDLP LAVEAMRRAI ARRPEHRVLW VATDGAQAVE LCAAQPPDVV LMDLIMPKFD 70 80 90 100 110 120 GIEATRRIMR SERPCAILIV TSCIGANAWR VFEAMGAGAL DAVDTPRLGD GAAGDTTKLL 130 140 150 160 170 180 LAKIDQIGRL LDAPGGTRLA GTAARAGGGP LIAIGASAGG PGALASILGG LPADFSAPIV 190 200 210 220 230 240 IVQHVDRAFA EGMAQWLDGQ TPLAVRVARE GDRPQPGVAL LAATDDHLRI TRAGTLEYTR 250 260 270 280 290 300 EPAATPYRPS VDVFFNSLTE HWPGRVIGVL LTGMGRDGAI GLKALRMKGY HTIAQDEATS 310 320 330 AVYGMPKAAA TLGAARAILP LGRIAGELAA LARI

References

[1]

"Complete sequence of chromosome 2 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000152 Genomic DNA. Translation: ABB12130.1.
RefSeq	YP_372774.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q394I6.
Genome annotation databases
GeneID	3753782.
GenomeReviews	Gene locus Bcep18194_B2017 in contig CP000152_GR.
KEGG	bur:Bcep18194_B2017.
NMPDR	fig|269483.3.peg.2024.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q394I6.
OMA	RPCAILI.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_B2017-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_BURS3

Accession

Primary (citable) accession number: Q394I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)