Q39491 (PTP3_CHLMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase
EC=3.1.3.16
EC=3.1.3.48

Gene names

Name:

VH-PTP13

Organism

Chlamydomonas moewusii (Chlamydomonas eugametos)

Taxonomic identifier

3054 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could be involved in tyrosine phosphatase signalling pathways, having MAP-kinases as substrates.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Developmental stage	Non-dividing gametes did not express the VH-PTP13 gene whereas synchronously dividing vegetative cells only expressed VH-PTP13 in the early G1-phase of the cycle.
Induction	By oxidative stress.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Ontologies

Keywords
Biological process	Stress response
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Dual specificity protein phosphatase		PRO_0000094873
Regions
	Region	89 – 276	188	Catalytic
Sites
	Active site	172	1	Phosphocysteine intermediate
Experimental info
	Mutagenesis	172	1	C → S: Complete loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q39491 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 034EF63951E03381	FASTA	276	30,310
	10 20 30 40 50 60 MTSHAANPKE EQDQRLPFKS DISGPIAPSQ APAHTVQAHD SQPKPSDDSS DEHLSFYEAK 70 80 90 100 110 120 ARIVRAFAAS RAPQPAAPAA KALEGDASVI VPGKLILSSC EVEESSELLT KLGVTHILQV 130 140 150 160 170 180 GEELKPSHPG RFTYLSLPIL DMEGQDIVAL LPSCFQFLQQ AQASGGVCLV HCLAGISRSA 190 200 210 220 230 240 SVVIAYLMWT QGMPYTEARA MVRRARSKVY PNTGFTLQLQ ELDRLRESGA IQWGDTPSLA 250 260 270 SSLEQHRQPW NLIRYLEVKE KQAQEEGWTW GRTLVI

References

[1]

"Tyrosine phosphatase signalling in a lower plant: cell-cycle and oxidative stress-regulated expression of the Chlamydomonas eugametos VH-PTP13 gene." Haring M.A., Siderius M., Jonak C., Hirt H., Walton K.M., Musgrave A. Plant J. 7:981-988(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF CYS-172. Strain: UTEX 10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77938 mRNA. Translation: CAA54910.1.
PIR	T48906.
3D structure databases
ProteinModelPortal	Q39491.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTP3_CHLMO

Accession

Primary (citable) accession number: Q39491

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program