ID   Q39476_CYNCA            Unreviewed;       509 AA.
AC   Q39476;
DT   01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 117.
DE   SubName: Full=Cyprosin {ECO:0000313|EMBL:CAA57510.1};
OS   Cynara cardunculus (Cardoon).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Carduoideae; Cardueae;
OC   Carduinae; Cynara.
OX   NCBI_TaxID=4265 {ECO:0000313|EMBL:CAA57510.1};
RN   [1] {ECO:0000313|EMBL:CAA57510.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Flower {ECO:0000313|EMBL:CAA57510.1};
RA   Pietrzak M., Brodelius P., Pais M.S.;
RL   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase A1 family.
CC       {ECO:0000256|ARBA:ARBA00007447, ECO:0000256|RuleBase:RU000454}.
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DR   EMBL; X81984; CAA57510.1; -; mRNA.
DR   PIR; S49349; S49349.
DR   AlphaFoldDB; Q39476; -.
DR   SMR; Q39476; -.
DR   MEROPS; A01.A02; -.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd06098; phytepsin; 1.
DR   Gene3D; 2.40.70.10; Acid Proteases; 2.
DR   Gene3D; 1.10.225.10; Saposin-like; 1.
DR   InterPro; IPR001461; Aspartic_peptidase_A1.
DR   InterPro; IPR001969; Aspartic_peptidase_AS.
DR   InterPro; IPR033121; PEPTIDASE_A1.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   InterPro; IPR033869; Phytepsin.
DR   InterPro; IPR007856; SapB_1.
DR   InterPro; IPR008138; SapB_2.
DR   InterPro; IPR011001; Saposin-like.
DR   InterPro; IPR008139; SaposinB_dom.
DR   PANTHER; PTHR47966; BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED; 1.
DR   PANTHER; PTHR47966:SF51; BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED; 1.
DR   Pfam; PF00026; Asp; 1.
DR   Pfam; PF05184; SapB_1; 1.
DR   Pfam; PF03489; SapB_2; 1.
DR   PRINTS; PR00792; PEPSIN.
DR   SMART; SM00741; SapB; 2.
DR   SUPFAM; SSF50630; Acid proteases; 1.
DR   SUPFAM; SSF47862; Saposin; 1.
DR   PROSITE; PS00141; ASP_PROTEASE; 2.
DR   PROSITE; PS51767; PEPTIDASE_A1; 1.
DR   PROSITE; PS50015; SAP_B; 2.
PE   2: Evidence at transcript level;
KW   Aspartyl protease {ECO:0000256|ARBA:ARBA00022750,
KW   ECO:0000256|RuleBase:RU000454};
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157,
KW   ECO:0000256|PIRSR:PIRSR601461-2};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000454};
KW   Protease {ECO:0000256|RuleBase:RU000454}; Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           25..509
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004222214"
FT   DOMAIN          85..506
FT                   /note="Peptidase A1"
FT                   /evidence="ECO:0000259|PROSITE:PS51767"
FT   DOMAIN          315..355
FT                   /note="Saposin B-type"
FT                   /evidence="ECO:0000259|PROSITE:PS50015"
FT   DOMAIN          379..420
FT                   /note="Saposin B-type"
FT                   /evidence="ECO:0000259|PROSITE:PS50015"
FT   ACT_SITE        103
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601461-1"
FT   ACT_SITE        290
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601461-1"
FT   DISULFID        116..122
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601461-2"
SQ   SEQUENCE   509 AA;  55177 MW;  4E287C423F827A44 CRC64;
     MGTAIKASVL ALFLFFLLSP TAFSVSNGGL LRVGLKKRKV DQINQLSGHG VSMEAKARKD
     FGFGGALRDS GSDIIALKNY MDAQYYGEIG IGSPPQKFTV IFDTGSSNLW VPSAKCYFSV
     ACLFHSKYKS SHSSTYKKNG TSAAIQYGTG SISGFVSQDS VKLGDLVVKE QDFIEATKEP
     GITFLAAKFD GILGLGFQEI SVGKSVPLWY NMVNQGLVQE PVFSFWFNRN ADEEEGGELV
     FGGVDPNHFK GKHTYVPVTE KGYWQFDMGD VLIEDKTTGF CSDGCAAIAD SGTSLLAGPT
     AIITEINHAI GAKGVMSQQC KTLVSQYGKT MIEMLLSEAQ PDKICSQMKL CTFDGARDAS
     SIIESVVDEN NGKSSSGVHD EMCTFCEMAV VWMQNQIKRN ETEDNIINYV NELCDRLPSP
     MGESAVDCNS LSSMPNIAFT IGGKVFELCP EQYILKIGEG EAAQCISGFT AMDVAPPRGP
     LWILGDVFMG RYHTVFDYGK LRVGFAEAA
//