Q39419 (POLC4_BETPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polcalcin Bet v 4

Alternative name(s):
Calcium-binding pollen allergen Bet v 4
Allergen=Bet v 4

Gene names

Name:

BETV4

Organism

Betula pendula (European white birch) (Betula verrucosa)

Taxonomic identifier

3505 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fagales › Betulaceae › Betula

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Monomer. Ref.3
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Disease	Allergen
Domain	Repeat
Ligand	Calcium Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 85

85

Polcalcin Bet v 4

PRO_0000073666

Regions

Domain

7 – 42

36

EF-hand 1

Domain

42 – 77

36

EF-hand 2

Calcium binding

20 – 31

12

1

Calcium binding

55 – 66

12

2

Experimental info

Sequence conflict

71

1

G → A in CAA73147. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

85

Helix Strand Turn

Details...

Helix

6 – 19

14

Beta strand

24 – 27

4

Helix

29 – 36

8

Helix

37 – 39

3

Helix

44 – 54

11

Helix

64 – 73

10

Helix

75 – 84

10

Sequences

	Sequence		Length	Mass (Da)
	Q39419 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E2FC882E5BB8ADFF	FASTA	85	9,448
	10 20 30 40 50 60 MADDHPQDKA ERERIFKRFD ANGDGKISAA ELGEALKTLG SITPDEVKHM MAEIDTDGDG 70 80 FISFQEFTDF GRANRGLLKD VAKIF

References

[1]	"Immunological and biological properties of Bet v 4, a novel birch pollen allergen with two EF-hand calcium-binding domains." Engel E., Richter K., Obermeyer G., Briza P., Kungl A.J., Simon B., Auer M., Ebner C., Rheinberger H.J., Breitenbach M., Ferreira F. J. Biol. Chem. 272:28630-28637(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.
[2]	"Molecular characterization, expression in Escherichia coli, and epitope analysis of a two EF-hand calcium-binding birch pollen allergen, Bet v 4." Twardosz A., Hayek B., Seiberler S., Pastore A., Vangelista L., Groenlund H., Kraft D., Valenta R. Biochem. Biophys. Res. Commun. 239:197-204(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.
[3]	"Solution structure, dynamics, and hydrodynamics of the calcium-bound cross-reactive birch pollen allergen Bet v 4 reveal a canonical monomeric two EF-hand assembly with a regulatory function." Neudecker P., Nerkamp J., Eisenmann A., Nourse A., Lauber T., Schweimer K., Lehmann K., Schwarzinger S., Ferreira F., Rosch P. J. Mol. Biol. 336:1141-1157(2004) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 2-85, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X87153 mRNA. Translation: CAA60628.1.
Y12560 mRNA. Translation: CAA73147.1.

PIR

JC5711.
S54819.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H4B	NMR	-	A	2-85	[»]

ProteinModelPortal

Q39419.

SMR

Q39419. Positions 2-85.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-7718600.

Protein family/group databases

Allergome

129. Bet v 4.
3138. Bet v 4.0101.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]

Pfam

PF00036. efhand. 2 hits.
[Graphical view]

SMART

SM00054. EFh. 2 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q39419.

Entry information

Entry name

POLC4_BETPN

Accession

Primary (citable) accession number: Q39419
Secondary accession number(s): O04131

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program