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Protein

Transcription factor CAULIFLOWER

Gene

CAL

Organism
Brassica oleracea var. italica (Broccoli)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1, FRUITFULL and LEAFY. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Seems to be partially redundant to the function of APETALA1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor CAULIFLOWER
Short name:
BoCAL
Short name:
BoiCAL
Alternative name(s):
Agamous-like MADS-box protein CAL
Gene namesi
Name:CAL
Synonyms:CAL1
OrganismiBrassica oleracea var. italica (Broccoli)
Taxonomic identifieri36774 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Transcription factor CAULIFLOWERPRO_0000417144Add
BLAST

Expressioni

Tissue specificityi

Expressed in some of the meristems of arrest-stage broccoli heads.1 Publication

Developmental stagei

Accumulates in early floral primordia and fades out in cells that will give rise to stamens and carpels.2 Publications

Interactioni

Subunit structurei

Homodimer capable of binding to CArG-box sequences.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ39375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6161MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini90 – 18091K-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGRVEMKR IENKINRQVT FSKRRAGLLK KAHEISILCD AEVSLIVFSH
60 70 80 90 100
KGKLFEYSSE SCMEKVLEHY ERYSYAEKQL KVPDSHVNAQ TNWSVEYSRL
110 120 130 140 150
KAKIELLERN QRHYLGEDLE SISIKELQNL EQQLDTSLKH IRSRKNQLMH
160 170 180 190 200
ESLNHLQRKE KEILEENSML AKQIRERESI LRTHQNQSEQ QNRSHHVAPQ
210 220 230 240 250
PQPQLNPYMA SSPFLNMGGM YQGEYPTAVR RNRLDLTLEP IYNCNLGYFA

A
Length:251
Mass (Da):29,524
Last modified:November 1, 1996 - v1
Checksum:i1E940476BC1E4032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36926 mRNA. Translation: AAA64790.1.
U67454 mRNA. Translation: AAB08878.1.
PIRiT14456.
UniGeneiBol.18949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36926 mRNA. Translation: AAA64790.1.
U67454 mRNA. Translation: AAB08878.1.
PIRiT14456.
UniGeneiBol.18949.

3D structure databases

ProteinModelPortaliQ39375.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular basis of the cauliflower phenotype in Arabidopsis."
    Kempin S.A., Savidge B., Yanofsky M.F.
    Science 267:522-525(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Flower meristem.
  2. "Floral homeotic gene expression defines developmental arrest stages in Brassica oleracea L. vars. botrytis and italica."
    Carr S.M., Irish V.F.
    Planta 201:179-188(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Flower.
  3. "Variation and selection at the CAULIFLOWER floral homeotic gene accompanying the evolution of domesticated Brassica oleracea."
    Purugganan M.D., Boyles A.L., Suddith J.I.
    Genetics 155:855-862(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, GENE FAMILY.
  4. "Meristem identity gene expression during curd proliferation and flower initiation in Brassica oleracea."
    Duclos D.V., Bjoerkman T.
    J. Exp. Bot. 59:421-433(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCAL_BRAOT
AccessioniPrimary (citable) accession number: Q39375
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1996
Last modified: December 9, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

CAULIFLOWER may contribute to the shape of the floral meristem. This trait has likely been selected by early farmers during the domestication of modified inflorescence structures.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.