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Protein

Argininosuccinate synthase

Gene

argG

Organism
Burkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF), Ornithine carbamoyltransferase (argF), Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG), Argininosuccinate synthase (argG), Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH), Argininosuccinate lyase (argH), Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei99CitrullineUniRule annotation1
Binding sitei129ATP; via amide nitrogenUniRule annotation1
Binding sitei131AspartateUniRule annotation1
Binding sitei131ATPUniRule annotation1
Binding sitei135AspartateUniRule annotation1
Binding sitei135CitrullineUniRule annotation1
Binding sitei136AspartateUniRule annotation1
Binding sitei136ATPUniRule annotation1
Binding sitei139CitrullineUniRule annotation1
Binding sitei192CitrullineUniRule annotation1
Binding sitei194ATPUniRule annotation1
Binding sitei201CitrullineUniRule annotation1
Binding sitei203CitrullineUniRule annotation1
Binding sitei280CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSP269483:GHLS-5819-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Bcep18194_B2399
OrganismiBurkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002705 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001297431 – 444Argininosuccinate synthaseAdd BLAST444

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ392V6.
SMRiQ392V6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.
OrthoDBiPOG091H01U0.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q392V6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTILESLPT GQKVGIAFSG GLDTSAALHW MKLKGAVPYA YTANLGQPDE
60 70 80 90 100
DDYDAIPKRA IEYGAAGARL IDCRAQLVAE GIAALQSGAF HITTAGVTYF
110 120 130 140 150
NTTPIGRAVT GTMLVAAMKE DGVNIWGDGS TYKGNDIERF YRYGLLVNPD
160 170 180 190 200
LKIYKPWLDQ TFIDELGGRA EMSEFMNQAG FAYKMSAEKA YSTDSNLLGA
210 220 230 240 250
THEAKDLESL ESGIKIVNPI MGVAFWRDDV KIAAEEVTVR FEAGQPVALN
260 270 280 290 300
GVEFSDPVEL LLEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGLALL
310 320 330 340 350
YIAYERLVTG IHNEDTIEQY RENGRRLGRL LYQGRWFDPQ AIMLRETAQR
360 370 380 390 400
WVARAITGEV KIELRRGNDY SILSTKSPNL TYQPERLSME KVASTFSPRD
410 420 430 440
RIGQLTMRNL DITDTRDKLR VYSQVGLLTP GETSALPQIK GDDK
Length:444
Mass (Da):49,108
Last modified:November 22, 2005 - v1
Checksum:iB6F045A9DCCB6AB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000152 Genomic DNA. Translation: ABB12510.1.
RefSeqiWP_011355992.1. NC_007511.1.

Genome annotation databases

EnsemblBacteriaiABB12510; ABB12510; Bcep18194_B2399.
KEGGibur:Bcep18194_B2399.
PATRICi19295711. VBIBurSp120713_7216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000152 Genomic DNA. Translation: ABB12510.1.
RefSeqiWP_011355992.1. NC_007511.1.

3D structure databases

ProteinModelPortaliQ392V6.
SMRiQ392V6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB12510; ABB12510; Bcep18194_B2399.
KEGGibur:Bcep18194_B2399.
PATRICi19295711. VBIBurSp120713_7216.

Phylogenomic databases

HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.
OrthoDBiPOG091H01U0.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciBSP269483:GHLS-5819-MONOMER.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_BURL3
AccessioniPrimary (citable) accession number: Q392V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.