Q39258 (VATE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type proton ATPase subunit E1
Short name=V-ATPase subunit E1

Alternative name(s):
Protein EMBRYO DEFECTIVE 2448
Vacuolar H(+)-ATPase subunit E isoform 1
Vacuolar proton pump subunit E1

Gene names

Name:	VHA-E1
Synonyms:	EMB2448, TUF, TUFF, VATE
Ordered Locus Names:	At4g11150
ORF Names:	F2P3.10, T22B4.130

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Required for Golgi organization and vacuole function in embryogenesis. Ref.6
Subunit structure	V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e).
Subcellular location	Vacuole membrane; Peripheral membrane protein Ref.7.
Developmental stage	Expressed mainly in the endosperm and surrounding maternal tissues during seed development. Ref.6
Disruption phenotype	Mutant embryos are lethal, displaying variably enlarged cells with multiple nuclei, large vacuoles containing inclusions, abnormal organization of Golgi stacks, and cell wall defects. Ref.6
Sequence similarities	Belongs to the V-ATPase E subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane Vacuole
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro Golgi organization Inferred from mutant phenotype Ref.6. Source: TAIR defense response to bacterium Inferred from expression pattern. Source: TAIR embryo development ending in seed dormancy Inferred from mutant phenotype Ref.6. Source: TAIR plant-type cell wall biogenesis Inferred from mutant phenotype Ref.6. Source: TAIR response to cold Inferred from expression pattern. Source: TAIR response to salt stress Inferred from expression pattern. Source: TAIR
Cellular component	cytosolic ribosome Inferred from direct assay. Source: TAIR plant-type vacuole membrane Inferred from direct assay Ref.6. Source: TAIR proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular function	proton-transporting ATPase activity, rotational mechanism Traceable author statement Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	V-type proton ATPase subunit E1		PRO_0000117301
Regions
	Coiled coil	8 – 67	60	Potential
	Compositional bias	173 – 176	4	Poly-Pro
Experimental info
	Sequence conflict	31	1	S → P in CAA63086. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q39258 [UniParc]. Last modified January 24, 2001. Version 2. Checksum: 633E1D8D78A0889B	FASTA	230	26,060
	10 20 30 40 50 60 MNDGDVSRQI QQMVRFIRQE AEEKANEISV SAEEEFNIEK LQLVEAEKKK IRQDYEKKEK 70 80 90 100 110 120 QADVRKKIDY SMQLNASRIK VLQAQDDIVN AMKDQAAKDL LNVSRDEYAY KQLLKDLIVQ 130 140 150 160 170 180 CLLRLKEPSV LLRCREEDLG LVEAVLDDAK EEYAGKAKVH APEVAVDTKI FLPPPPKSND 190 200 210 220 230 PHGLHCSGGV VLASRDGKIV CENTLDARLD VAFRMKLPVI RKSLFGQVTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of subunit E of the vacuolar proton-ATPase of Spinacia oleracea and Arabidopsis thaliana." Dietz K.-J., Hollenbach B., Arnold J. Plant Gene Register PGR96-037 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase." Sze H., Schumacher K., Mueller M.L., Padmanaban S., Taiz L. Trends Plant Sci. 7:157-161(2002) [PubMed: 11950611] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Arabidopsis vacuolar H(+)-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis." Strompen G., Dettmer J., Stierhof Y.-D., Schumacher K., Juergens G., Mayer U. Plant J. 41:125-132(2005) [PubMed: 15610355] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[7]	"Mapping the Arabidopsis organelle proteome." Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S. Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed: 16618929] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92117 mRNA. Translation: CAA63086.1. AF080120 Genomic DNA. Translation: AAC35545.1. AL049876 Genomic DNA. Translation: CAB43050.1. AL161531 Genomic DNA. Translation: CAB81216.1. CP002687 Genomic DNA. Translation: AEE82978.1. AY065119 mRNA. Translation: AAL38295.1. AY081632 mRNA. Translation: AAM10194.1.
IPI	IPI00541951.
PIR	T01918.
RefSeq	NP_192853.1. NM_117185.4.
UniGene	At.23873.
3D structure databases
ProteinModelPortal	Q39258.
SMR	Q39258. Positions 1-62, 87-226.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39258. 1 interaction.
STRING	Q39258.
Protein family/group databases
TCDB	3.A.2.2.5. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
2D gel databases
World-2DPAGE	0003:Q39258.
Proteomic databases
PRIDE	Q39258.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G11150.1; AT4G11150.1; AT4G11150.
GeneID	826716.
GenomeReviews	Gene locus AT4G11150 in contig CT486007_GR.
KEGG	ath:AT4G11150.
NMPDR	fig|3702.1.peg.18781.
Organism-specific databases
TAIR	At4g11150.
Phylogenomic databases
eggNOG	KOG1664.
GeneTree	EPGT00070000028960.
HOGENOM	HBG525538.
InParanoid	Q39258.
OMA	QQRQKIM.
PhylomeDB	Q39258.
ProtClustDB	CLSN2682633.
Gene expression databases
ArrayExpress	Q39258.
Genevestigator	Q39258.
GermOnline	AT4G11150. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002842. ATPase_V1/A1-cplx_esu. [Graphical view]
KO	K02150.
Pfam	PF01991. vATP-synt_E. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VATE1_ARATH

Accession

Primary (citable) accession number: Q39258
Secondary accession number(s): O82502

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 24, 2001
Last modified:	November 16, 2011
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program