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Protein

SKP1-like protein 1A

Gene

SKP1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.6 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB-KW
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • microtubule cytoskeleton organization Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of DNA recombination Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro
  • viral process Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein
Biological processAuxin signaling pathway, Chromosome partition, Ethylene signaling pathway, Host-virus interaction, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-8951664. Neddylation.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SKP1-like protein 1A1 Publication
Short name:
SKP1-like 11 Publication
Alternative name(s):
UFO-binding protein 11 Publication
Gene namesi
Name:SKP1A1 Publication
Synonyms:ASK11 Publication, SKP11 Publication, UIP11 Publication
Ordered Locus Names:At1g75950Imported
ORF Names:T4O12.17Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G75950.
TAIRilocus:2204435. AT1G75950.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872551 – 160SKP1-like protein 1AAdd BLAST160

Proteomic databases

PaxDbiQ39255.
PRIDEiQ39255.

PTM databases

iPTMnetiQ39255.
SwissPalmiQ39255.

Expressioni

Tissue specificityi

Accumulates only in meristematic cells. Expressed in inflorescence, shoot and root apical meristems, as well as in developing organs such as gametocytes and seeds. Also detected in cortical layer and epidermis of roots, leaves, pith and vascular bundle of young stem, young floral buds and organ primordia, pollen and through the valve of siliques. Not detectable in mature root tissues.5 Publications

Developmental stagei

During flower development, expressed in the tapetal layer surrounding the male microsporocytes, and in the endothelium layer surrounding the embryo sac within the ovule. During embryogenesis, expressed in all cells of the embryo and in developing endosperm surrounding the embryo, at the time of free nuclei proliferation.1 Publication

Gene expression databases

ExpressionAtlasiQ39255. baseline and differential.
GenevisibleiQ39255. AT.

Interactioni

Subunit structurei

Part of a SCF E3 ubiquitin ligase complex composed of SKP1, CUL1, RBX1 (RBX1A or RBX1B) and F-box proteins. Interacts with SKIP1, SKIP2, SKIP3, SKIP4, SKIP6, FIB1/SKIP7, SKIP8, PP2A11/SKIP10, SKIP11, PP2B11/SKIP12, PP2A14/SKIP13, SKIP14, SKIP15, SKIP16, SKIP19/FBL20, SKIP20, PP2B1/SKIP21, SKIP22, SKIP23, SKIP24, SKIP25, TULP10/SKIP26, SKIP27, SKIP28/MEE11, AFR/SKIP29, SKIP30, SKIP31, SKIP32/FBP7, SKIP33, SKIP35, ADO1/ZTL, ADO2/LKP2, ADO3/FKF1, AFR, COI1, DOR, EBF1, EBF2, EID1, ORE9, PP2A13/SKIP9, TIR1, UFO, SKP2A, CPR30, FBL17, NUP58, At1g55000, At1g67340, At1g78100, At3g04660, At3g61590, At4g38940 and At5g49610. The SKP1A subunit of the SCF E3 ubiquitin ligase complex can interact directly with KIN10, KIN11 and the proteasome subunit PAD1. This interaction can be disrupted by PRL1. In case of polerovirus infection, part of a SCF P0 complex composed of the viral silencing suppressor P0, SKP1 and CUL1. Interacts with turnip yellows virus P0. Interacts with VBF and Agrobacterium virF. Binds to KIB1 (PubMed:28575660).28 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi29147. 128 interactors.
DIPiDIP-31325N.
IntActiQ39255. 68 interactors.
STRINGi3702.AT1G75950.1.

Structurei

Secondary structure

1160
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Beta strandi11 – 13Combined sources3
Beta strandi15 – 18Combined sources4
Helixi21 – 24Combined sources4
Turni27 – 29Combined sources3
Turni31 – 36Combined sources6
Helixi37 – 40Combined sources4
Helixi49 – 57Combined sources9
Turni80 – 85Combined sources6
Beta strandi89 – 91Combined sources3
Beta strandi94 – 96Combined sources3
Helixi102 – 107Combined sources6
Helixi111 – 123Combined sources13
Turni124 – 127Combined sources4
Helixi130 – 136Combined sources7
Helixi145 – 159Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P1MX-ray1.80A1-160[»]
2P1NX-ray2.50A/D1-160[»]
2P1OX-ray1.90A1-160[»]
2P1PX-ray2.21A1-160[»]
2P1QX-ray1.91A1-160[»]
3C6NX-ray2.60A1-160[»]
3C6OX-ray2.70A1-160[»]
3C6PX-ray2.70A1-160[»]
3OGKX-ray2.80A/C/E/G/I/K/M/O1-160[»]
3OGLX-ray3.18A/C/E/G/I/K/M/O1-160[»]
3OGMX-ray3.34A/C/E/G/I/K/M/O1-160[»]
5HYWX-ray3.01B/D1-160[»]
5HZGX-ray3.30C/G1-160[»]
ProteinModelPortaliQ39255.
SMRiQ39255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ39255.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 160Interaction with the F-box domain of F-box proteinsAdd BLAST59

Sequence similaritiesi

Belongs to the SKP1 family.Curated

Phylogenomic databases

eggNOGiKOG1724. Eukaryota.
COG5201. LUCA.
HOGENOMiHOG000172184.
InParanoidiQ39255.
KOiK03094.
OMAiAWDADFM.
OrthoDBiEOG09360RN0.
PhylomeDBiQ39255.

Family and domain databases

InterProiView protein in InterPro
IPR016897. SKP1.
IPR001232. SKP1-like.
IPR011333. SKP1/BTB/POZ.
IPR016072. Skp1_comp_dimer.
IPR016073. Skp1_comp_POZ.
PfamiView protein in Pfam
PF01466. Skp1. 1 hit.
PF03931. Skp1_POZ. 1 hit.
PIRSFiPIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit.
SMARTiView protein in SMART
SM00512. Skp1. 1 hit.
SUPFAMiSSF54695. SSF54695. 1 hit.
SSF81382. SSF81382. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKKIVLKS SDGESFEVEE AVALESQTIA HMVEDDCVDN GVPLPNVTSK
60 70 80 90 100
ILAKVIEYCK RHVEAAASKA EAVEGAATSD DDLKAWDADF MKIDQATLFE
110 120 130 140 150
LILAANYLNI KNLLDLTCQT VADMIKGKTP EEIRTTFNIK NDFTPEEEEE
160
VRRENQWAFE
Length:160
Mass (Da):17,857
Last modified:November 1, 1996 - v1
Checksum:i56B84C19FD5ABF17
GO

Sequence cautioni

The sequence AAB38862 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43P → L in AAB38862 (PubMed:9530878).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60981 mRNA. Translation: AAB17535.1.
U97020 mRNA. Translation: AAC63109.1.
AF059294 mRNA. Translation: AAC14444.1.
AC007396 Genomic DNA. Translation: AAF26761.1.
CP002684 Genomic DNA. Translation: AEE35780.1.
AY080884 mRNA. Translation: AAL87354.1.
AY113971 mRNA. Translation: AAM45019.1.
U70034 mRNA. Translation: AAB38862.1. Different initiation.
PIRiT51309.
RefSeqiNP_565123.1. NM_106245.5.
UniGeneiAt.23531.

Genome annotation databases

EnsemblPlantsiAT1G75950.1; AT1G75950.1; AT1G75950.
GeneIDi843928.
GrameneiAT1G75950.1; AT1G75950.1; AT1G75950.
KEGGiath:AT1G75950.

Similar proteinsi

Entry informationi

Entry nameiSKP1A_ARATH
AccessioniPrimary (citable) accession number: Q39255
Secondary accession number(s): P92992
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families