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Protein

SKP1-like protein 1A

Gene

SKP1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.6 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB-KW
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • microtubule cytoskeleton organization Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of DNA recombination Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Auxin signaling pathway, Chromosome partition, Ethylene signaling pathway, Host-virus interaction, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SKP1-like protein 1A
Short name:
SKP1-like 1
Alternative name(s):
UFO-binding protein 1
Gene namesi
Name:SKP1A
Synonyms:ASK1, SKP1, UIP1
Ordered Locus Names:At1g75950
ORF Names:T4O12.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G75950.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • phragmoplast Source: TAIR
  • plasma membrane Source: TAIR
  • spindle Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160SKP1-like protein 1APRO_0000187255Add
BLAST

Proteomic databases

PaxDbiQ39255.
PRIDEiQ39255.

PTM databases

iPTMnetiQ39255.
SwissPalmiQ39255.

Expressioni

Tissue specificityi

Accumulates only in meristematic cells. Expressed in inflorescence, shoot and root apical meristems, as well as in developing organs such as gametocytes and seeds. Also detected in cortical layer and epidermis of roots, leaves, pith and vascular bundle of young stem, young floral buds and organ primordia, pollen and through the valve of siliques. Not detectable in mature root tissues.5 Publications

Developmental stagei

During flower development, expressed in the tapetal layer surrounding the male microsporocytes, and in the endothelium layer surrounding the embryo sac within the ovule. During embryogenesis, expressed in all cells of the embryo and in developing endosperm surrounding the embryo, at the time of free nuclei proliferation.1 Publication

Gene expression databases

GenevisibleiQ39255. AT.

Interactioni

Subunit structurei

Part of a SCF E3 ubiquitin ligase complex composed of SKP1, CUL1, RBX1 (RBX1A or RBX1B) and F-box proteins. Interacts with SKIP1, SKIP2, SKIP3, SKIP4, SKIP6, FIB1/SKIP7, SKIP8, PP2A11/SKIP10, SKIP11, PP2B11/SKIP12, PP2A14/SKIP13, SKIP14, SKIP15, SKIP16, SKIP19/FBL20, SKIP20, PP2B1/SKIP21, SKIP22, SKIP23, SKIP24, SKIP25, TULP10/SKIP26, SKIP27, SKIP28/MEE11, AFR/SKIP29, SKIP30, SKIP31, SKIP32/FBP7, SKIP33, SKIP35, ADO1/ZTL, ADO2/LKP2, ADO3/FKF1, AFR, COI1, DOR, EBF1, EBF2, EID1, ORE9, PP2A13/SKIP9, TIR1, UFO, SKP2A, CPR30, FBL17, NUP58, At1g55000, At1g67340, At1g78100, At3g04660, At3g61590, At4g38940 and At5g49610. The SKP1A subunit of the SCF E3 ubiquitin ligase complex can interact directly with KIN10, KIN11 and the proteasome subunit PAD1. This interaction can be disrupted by PRL1. In case of polerovirus infection, part of a SCF P0 complex composed of the viral silencing suppressor P0, SKP1 and CUL1. Interacts with turnip yellows virus P0. Interacts with VBF and Agrobacterium virF.27 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Q659673EBI-532357,EBI-848577From a different organism.
ADO1Q94BT69EBI-532357,EBI-300691
AFRQ8LAW24EBI-532357,EBI-604438
COI1O041977EBI-532357,EBI-401159
CUL1Q94AH67EBI-532357,EBI-532411
EBF1Q9SKK04EBI-532357,EBI-401198
EID1Q8LEA84EBI-532357,EBI-687388
GRH1Q9ZR123EBI-532357,EBI-617479
IAA7Q388252EBI-532357,EBI-602959
PP2A13Q9LEX04EBI-532357,EBI-604261
SKIP1Q9FDX15EBI-532357,EBI-604228
SKIP15O492794EBI-532357,EBI-591174
SKIP2Q9FE833EBI-532357,EBI-591107
SKIP28Q9ZU903EBI-532357,EBI-604427
TIR1Q570C04EBI-532357,EBI-307183
UFOQ390906EBI-532357,EBI-590758
virFP155976EBI-532357,EBI-605118From a different organism.

Protein-protein interaction databases

BioGridi29147. 127 interactions.
DIPiDIP-31325N.
IntActiQ39255. 68 interactions.
STRINGi3702.AT1G75950.1.

Structurei

Secondary structure

1
160
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 94Combined sources
Beta strandi11 – 133Combined sources
Beta strandi15 – 184Combined sources
Helixi21 – 244Combined sources
Turni27 – 293Combined sources
Turni31 – 366Combined sources
Helixi37 – 404Combined sources
Helixi49 – 579Combined sources
Beta strandi89 – 913Combined sources
Helixi102 – 1076Combined sources
Helixi111 – 12313Combined sources
Turni124 – 1274Combined sources
Helixi130 – 1367Combined sources
Helixi145 – 15915Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P1MX-ray1.80A1-160[»]
2P1NX-ray2.50A/D1-160[»]
2P1OX-ray1.90A1-160[»]
2P1PX-ray2.21A1-160[»]
2P1QX-ray1.91A1-160[»]
3C6NX-ray2.60A1-160[»]
3C6OX-ray2.70A1-160[»]
3C6PX-ray2.70A1-160[»]
3OGKX-ray2.80A/C/E/G/I/K/M/O1-160[»]
3OGLX-ray3.18A/C/E/G/I/K/M/O1-160[»]
3OGMX-ray3.34A/C/E/G/I/K/M/O1-160[»]
ProteinModelPortaliQ39255.
SMRiQ39255. Positions 5-160.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ39255.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 16059Interaction with the F-box domain of F-box proteinsAdd
BLAST

Sequence similaritiesi

Belongs to the SKP1 family.Curated

Phylogenomic databases

eggNOGiKOG1724. Eukaryota.
COG5201. LUCA.
HOGENOMiHOG000172184.
InParanoidiQ39255.
KOiK03094.
OMAiPQEDENK.
OrthoDBiEOG09360RN0.
PhylomeDBiQ39255.

Family and domain databases

InterProiIPR016897. SKP1.
IPR001232. SKP1-like.
IPR011333. SKP1/BTB/POZ.
IPR016072. Skp1_comp_dimer.
IPR016073. Skp1_comp_POZ.
[Graphical view]
PfamiPF01466. Skp1. 1 hit.
PF03931. Skp1_POZ. 1 hit.
[Graphical view]
PIRSFiPIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit.
SMARTiSM00512. Skp1. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
SSF81382. SSF81382. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKKIVLKS SDGESFEVEE AVALESQTIA HMVEDDCVDN GVPLPNVTSK
60 70 80 90 100
ILAKVIEYCK RHVEAAASKA EAVEGAATSD DDLKAWDADF MKIDQATLFE
110 120 130 140 150
LILAANYLNI KNLLDLTCQT VADMIKGKTP EEIRTTFNIK NDFTPEEEEE
160
VRRENQWAFE
Length:160
Mass (Da):17,857
Last modified:November 1, 1996 - v1
Checksum:i56B84C19FD5ABF17
GO

Sequence cautioni

The sequence AAB38862 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431P → L in AAB38862 (PubMed:9530878).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60981 mRNA. Translation: AAB17535.1.
U97020 mRNA. Translation: AAC63109.1.
AF059294 mRNA. Translation: AAC14444.1.
AC007396 Genomic DNA. Translation: AAF26761.1.
CP002684 Genomic DNA. Translation: AEE35780.1.
AY080884 mRNA. Translation: AAL87354.1.
AY113971 mRNA. Translation: AAM45019.1.
U70034 mRNA. Translation: AAB38862.1. Different initiation.
PIRiT51309.
RefSeqiNP_565123.1. NM_106245.4.
UniGeneiAt.23531.

Genome annotation databases

EnsemblPlantsiAT1G75950.1; AT1G75950.1; AT1G75950.
GeneIDi843928.
GrameneiAT1G75950.1; AT1G75950.1; AT1G75950.
KEGGiath:AT1G75950.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60981 mRNA. Translation: AAB17535.1.
U97020 mRNA. Translation: AAC63109.1.
AF059294 mRNA. Translation: AAC14444.1.
AC007396 Genomic DNA. Translation: AAF26761.1.
CP002684 Genomic DNA. Translation: AEE35780.1.
AY080884 mRNA. Translation: AAL87354.1.
AY113971 mRNA. Translation: AAM45019.1.
U70034 mRNA. Translation: AAB38862.1. Different initiation.
PIRiT51309.
RefSeqiNP_565123.1. NM_106245.4.
UniGeneiAt.23531.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P1MX-ray1.80A1-160[»]
2P1NX-ray2.50A/D1-160[»]
2P1OX-ray1.90A1-160[»]
2P1PX-ray2.21A1-160[»]
2P1QX-ray1.91A1-160[»]
3C6NX-ray2.60A1-160[»]
3C6OX-ray2.70A1-160[»]
3C6PX-ray2.70A1-160[»]
3OGKX-ray2.80A/C/E/G/I/K/M/O1-160[»]
3OGLX-ray3.18A/C/E/G/I/K/M/O1-160[»]
3OGMX-ray3.34A/C/E/G/I/K/M/O1-160[»]
ProteinModelPortaliQ39255.
SMRiQ39255. Positions 5-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29147. 127 interactions.
DIPiDIP-31325N.
IntActiQ39255. 68 interactions.
STRINGi3702.AT1G75950.1.

PTM databases

iPTMnetiQ39255.
SwissPalmiQ39255.

Proteomic databases

PaxDbiQ39255.
PRIDEiQ39255.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G75950.1; AT1G75950.1; AT1G75950.
GeneIDi843928.
GrameneiAT1G75950.1; AT1G75950.1; AT1G75950.
KEGGiath:AT1G75950.

Organism-specific databases

TAIRiAT1G75950.

Phylogenomic databases

eggNOGiKOG1724. Eukaryota.
COG5201. LUCA.
HOGENOMiHOG000172184.
InParanoidiQ39255.
KOiK03094.
OMAiPQEDENK.
OrthoDBiEOG09360RN0.
PhylomeDBiQ39255.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTraceiQ39255.
PROiQ39255.

Gene expression databases

GenevisibleiQ39255. AT.

Family and domain databases

InterProiIPR016897. SKP1.
IPR001232. SKP1-like.
IPR011333. SKP1/BTB/POZ.
IPR016072. Skp1_comp_dimer.
IPR016073. Skp1_comp_POZ.
[Graphical view]
PfamiPF01466. Skp1. 1 hit.
PF03931. Skp1_POZ. 1 hit.
[Graphical view]
PIRSFiPIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit.
SMARTiSM00512. Skp1. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
SSF81382. SSF81382. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSKP1A_ARATH
AccessioniPrimary (citable) accession number: Q39255
Secondary accession number(s): P92992
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.