Reviewed,
UniProtKB/Swiss-Prot Q39255 (SKP1A_ARATH)
Last modified
November 25, 2008.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: SKP1-like protein 1A Short name=SKP1-like 1 Alternative name(s): UFO-binding protein 1 | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 160 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation. |
| Pathway | |
| Subunit structure | Part of a SCF E3 ubiquitin ligase complex composed of SKP1, CUL1, RBX1 (RBX1A or RBX1B) and F-box proteins such as UFO, EID1, AFR, ORE9, ADO1/ZTL, ADO2/LKP2, ADO3/FKF1, EBF1, EBF2, COI1 and TIR1. The SKP1A subunit of the SCF E3 ubiquitin ligase complex can interact directly with KIN10, KIN11 and the proteasome subunit PAD1. This interaction can be disrupted by PRL1. Also interacts with SKIP1, SKIP2, SKIP3, SKIP4, SKIP6 and FIB1/SKIP7. |
| Subcellular location | Nucleus. Spindle. Cytoplasm › cytoskeleton › phragmoplast. Note= Associated to mitotic spindle and phragmoplasts during cell division. |
| Tissue specificity | Accumulates only in meristematic cells. Expressed in inflorescence, shoot and root apical meristems, as well as in developing organs such as gametocytes and seeds. Also detected in cortical layer and epidermis of roots, leaves, pith and vascular bundle of young stem, young floral buds and organ primordia, pollen and through the valve of siliques. Not detectable in mature root tissues. |
| Developmental stage | During flower development, expressed in the tapetal layer surrounding the male microsporocytes, and in the endothelium layer surrounding the embryo sac within the ovule. During embryogenesis, expressed in all cells of the embryo and in developing endosperm surrounding the embryo, at the time of free nuclei proliferation. |
| Sequence similarities | Belongs to the SKP1 family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Q65967 | 1 | EBI-532357,EBI-848577 | From a different organism. | |
| Q9LU91 | 2 | EBI-532357,EBI-604313 | ||
| ADO1 | Q94BT6 | 4 | EBI-532357,EBI-300691 | |
| AFR | Q8LAW2 | 3 | EBI-532357,EBI-604438 | |
| At2g01620/T8O11.21 | Q9ZU90 | 2 | EBI-532357,EBI-604427 | |
| COI1 | O04197 | 6 | EBI-532357,EBI-401159 | |
| CUL1 | Q94AH6 | 4 | EBI-532357,EBI-532411 | |
| EBF1 | Q9SKK0 | 3 | EBI-532357,EBI-401198 | |
| EID1 | Q8LEA8 | 2 | EBI-532357,EBI-687388 | |
| FBP7 | Q9XI00 | 2 | EBI-532357,EBI-591127 | |
| FBX3 | O49279 | 2 | EBI-532357,EBI-591174 | |
| IAA7 | Q38825 | 2 | EBI-532357,EBI-602959 | |
| PP2A13 | Q9LEX0 | 3 | EBI-532357,EBI-604261 | |
| PP2A14 | Q9FJ80 | 2 | EBI-532357,EBI-604303 | |
| SKIP1 | Q9FDX1 | 2 | EBI-532357,EBI-604228 | |
| TIR1 | Q570C0 | 4 | EBI-532357,EBI-307183 | |
| UFO | Q39090 | 4 | EBI-532357,EBI-590758 | |
| virF | P15597 | 5 | EBI-532357,EBI-605118 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 160 | 160 | SKP1-like protein 1A | PRO_0000187255 | ||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||
| Sequence conflict | 43 | 1 | P → L in AAB38862. Ref.6 | |||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||
| Helix | 21 – 24 | 4 | ||||||||||||||||||||||
| Turn | 27 – 29 | 3 | ||||||||||||||||||||||
| Helix | 49 – 57 | 9 | ||||||||||||||||||||||
| Helix | 102 – 107 | 6 | ||||||||||||||||||||||
| Helix | 111 – 123 | 13 | ||||||||||||||||||||||
| Turn | 124 – 127 | 4 | ||||||||||||||||||||||
| Helix | 130 – 136 | 7 | ||||||||||||||||||||||
| Helix | 145 – 159 | 15 | ||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression." Connelly C., Hieter P. Cell 86:275-285(1996) [PubMed: 8706132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The UNUSUAL FLORAL ORGANS gene of Arabidopsis thaliana is an F-box protein required for normal patterning and growth in the floral meristem." Samach A., Klenz J.E., Kohalmi S.E., Risseeuw E., Haughn G.W., Crosby W.L. Plant J. 20:433-445(1999) [PubMed: 10607296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH UFO. Strain: cv. Columbia. |
| [3] | "Overexpression of Arabidopsis thaliana SKP1 homologues in yeast inactivates the Mig1 repressor by destabilising the F-box protein Grr1." Schouten J., de Kam R.J., Fetter K., Hoge J.H.C. Mol. Gen. Genet. 263:309-319(2000) [PubMed: 10778750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta. |
| [4] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [5] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [6] | "Arabidopsis SKP1, a homologue of a cell cycle regulator gene, is predominantly expressed in meristematic cells." Porat R., Lu P., O'Neill S.D. Planta 204:345-351(1998) [PubMed: 9530878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-160, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: cv. Columbia. |
| [7] | "The ASK1 gene regulates development and interacts with the UFO gene to control floral organ identity in Arabidopsis." Zhao D., Yang M., Solava J., Ma H. Dev. Genet. 25:209-223(1999) [PubMed: 10528262] [Abstract] Cited for: FUNCTION. |
| [8] | "Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana." Gray W.M., del Pozo J.C., Walker L., Hobbie L., Risseeuw E., Banks T., Crosby W.L., Yang M., Ma H., Estelle M. Genes Dev. 13:1678-1691(1999) [PubMed: 10398681] [Abstract] Cited for: FUNCTION, INTERACTION WITH TIR1. |
| [9] | "The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation." Yang M., Hu Y., Lodhi M., McCombie W.R., Ma H. Proc. Natl. Acad. Sci. U.S.A. 96:11416-11421(1999) [PubMed: 10500191] [Abstract] Cited for: FUNCTION. |
| [10] | "The ASK1 gene regulates B function gene expression in cooperation with UFO and LEAFY in Arabidopsis." Zhao D., Yu Q., Chen M., Ma H. Development 128:2735-2746(2001) [PubMed: 11526079] [Abstract] Cited for: FUNCTION. |
| [11] | "SKP1-SnRK protein kinase interactions mediate proteasomal binding of a plant SCF ubiquitin ligase." Farras R., Ferrando A., Jasik J., Kleinow T., Oekresz L., Tiburcio A., Salchert K., del Pozo C., Schell J., Koncz C. EMBO J. 20:2742-2756(2001) [PubMed: 11387208] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SKIP1; SKIP2; SKIP3; SKIP4; SKIP6; FIB1; CUL1; PAD1; KIN10 AND KIN11. |
| [12] | "EID1, an F-box protein involved in phytochrome A-specific light signaling." Dieterle M., Zhou Y.-C., Schaefer E., Funk M., Kretsch T. Genes Dev. 15:939-944(2001) [PubMed: 11316788] [Abstract] Cited for: INTERACTION WITH EID1. |
| [13] | "ORE9, an F-box protein that regulates leaf senescence in Arabidopsis." Woo H.R., Chung K.M., Park J.-H., Oh S.A., Ahn T., Hong S.H., Jang S.K., Nam H.G. Plant Cell 13:1779-1790(2001) [PubMed: 11487692] [Abstract] Cited for: INTERACTION WITH ORE9. |
| [14] | "The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects." Lechner E., Xie D., Grava S., Pigaglio E., Planchais S., Murray J.A.H., Parmentier Y., Mutterer J., Dubreucq B., Shen W.-H., Genschik P. J. Biol. Chem. 277:50069-50080(2002) [PubMed: 12381738] [Abstract] Cited for: IDENTIFICATION IN A SCF COMPLEX. |
| [15] | "The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis." Xu L., Liu F., Lechner E., Genschik P., Crosby W.L., Ma H., Peng W., Huang D., Xie D. Plant Cell 14:1919-1935(2002) [PubMed: 12172031] [Abstract] Cited for: INTERACTION WITH COI1, IDENTIFICATION IN A SCF(COI1) COMPLEX. |
| [16] | "COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis." Devoto A., Nieto-Rostro M., Xie D., Ellis C., Harmston R., Patrick E., Davis J., Sherratt L., Coleman M., Turner J.G. Plant J. 32:457-466(2002) [PubMed: 12445118] [Abstract] Cited for: INTERACTION WITH COI1, IDENTIFICATION IN A SCF(COI1) COMPLEX. |
| [17] | "Plant responses to ethylene gas are mediated by SCF(EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor." Guo H., Ecker J.R. Cell 115:667-677(2003) [PubMed: 14675532] [Abstract] Cited for: INTERACTION WITH EBF1 AND EBF2. |
| [18] | "The F box protein AFR is a positive regulator of phytochrome A-mediated light signaling." Harmon F.G., Kay S.A. Curr. Biol. 13:2091-2096(2003) [PubMed: 14653999] [Abstract] Cited for: INTERACTION WITH AFR. |
| [19] | "The F-box-containing protein UFO and AGAMOUS participate in antagonistic pathways governing early petal development in Arabidopsis." Durfee T., Roe J.L., Sessions R.A., Inouye C., Serikawa K., Feldmann K.A., Weigel D., Zambryski P.C. Proc. Natl. Acad. Sci. U.S.A. 100:8571-8576(2003) [PubMed: 12826617] [Abstract] Cited for: INTERACTION WITH UFO. |
| [20] | "Members of the Arabidopsis-SKP1-like gene family exhibit a variety of expression patterns and may play diverse roles in Arabidopsis." Zhao D., Ni W., Feng B., Han T., Petrasek M.G., Ma H. Plant Physiol. 133:203-217(2003) [PubMed: 12970487] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY. |
| [21] | "Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH ADO1; ADO2 AND ADO3. |
| [22] | "The ASK1 and ASK2 genes are essential for Arabidopsis early development." Liu F., Ni W., Griffith M.E., Huang Z., Chang C., Peng W., Ma H., Xie D. Plant Cell 16:5-20(2004) [PubMed: 14688296] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY. |
| [23] | "Formation of an SCF(ZTL) complex is required for proper regulation of circadian timing." Han L., Mason M., Risseeuw E.P., Crosby W.L., Somers D.E. Plant J. 40:291-301(2004) [PubMed: 15447654] [Abstract] Cited for: INTERACTION WITH ADO1. |
| + | Additional computationally mapped references. |
Web resources
| PlantsUBQ A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U60981 mRNA. Translation: AAB17535.1. U97020 mRNA. Translation: AAC63109.1. AF059294 mRNA. Translation: AAC14444.1. AC007396 Genomic DNA. Translation: AAF26761.1. AY080884 mRNA. Translation: AAL87354.1. AY113971 mRNA. Translation: AAM45019.1. U70034 mRNA. Translation: AAB38862.1. Different initiation. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PIR | T51309. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| RefSeq | NP_565123.1. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| UniGene | At.23531 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| IntAct | Q39255. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GeneID | 843928. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GenomeReviews | Gene locus AT1G75950 in contig CT485782_GR. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| KEGG | ath:AT1G75950. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NMPDR | fig|3702.1.peg.7113. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GeneFarm | 4981. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| TAIR | At1g75950. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ArrayExpress | Q39255. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GermOnline | AT1G75950. Arabidopsis thaliana. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| InterPro | IPR011333. BTB/POZ_fold. IPR016897. E3_ubiquit_lig_SCF_Skp. IPR001232. Skp1_comp. IPR016072. Skp1_comp_dimer. IPR016073. Skp1_comp_POZ. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Gene3D | G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PANTHER | PTHR11165. Skp1. 1 hit. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Pfam | PF01466. Skp1. 1 hit. PF03931. Skp1_POZ. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PIRSF | PIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| SMART | SM00512. Skp1. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | SKP1A_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q39255 Secondary accession number(s): P92992 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


