Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thioredoxin reductase 1, mitochondrial

Gene

NTR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses thioredoxin-disulfide reductase activity towards thioredoxins O1, O2 and F3.1 Publication

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.

Cofactori

FADNote: Binds 1 FAD per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101FAD1 Publication1
Binding sitei134FAD; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei192FAD1 Publication1
Binding sitei337FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 61FAD1 Publication4
Nucleotide bindingi79 – 80FAD1 Publication2
Nucleotide bindingi87 – 92FAD1 Publication6
Nucleotide bindingi344 – 346FAD1 Publication3

GO - Molecular functioni

  • thioredoxin-disulfide reductase activity Source: TAIR

GO - Biological processi

  • positive regulation of cell division Source: TAIR
  • removal of superoxide radicals Source: InterPro

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciARA:AT4G35460-MONOMER
BRENDAi1.8.1.9 399

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin reductase 1, mitochondrial (EC:1.8.1.9)
Alternative name(s):
NADPH-dependent thioredoxin reductase 1
Short name:
NTR1
NADPH-dependent thioredoxin reductase B
Short name:
AtNTRB
Gene namesi
Name:NTR1
Synonyms:NTRB
Ordered Locus Names:At4g35460
ORF Names:F15J1.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G35460
TAIRilocus:2117612 AT4G35460

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37Mitochondrion1 PublicationAdd BLAST37
ChainiPRO_000016677138 – 375Thioredoxin reductase 1, mitochondrialAdd BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi189 ↔ 192Redox-active1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ39243
PRIDEiQ39243

PTM databases

iPTMnetiQ39243

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

ExpressionAtlasiQ39243 baseline and differential
GenevisibleiQ39243 AT

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT4G35460.1

Structurei

Secondary structure

1375
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi47 – 56Combined sources10
Helixi60 – 71Combined sources12
Beta strandi77 – 79Combined sources3
Beta strandi82 – 84Combined sources3
Helixi92 – 95Combined sources4
Beta strandi97 – 99Combined sources3
Helixi111 – 124Combined sources14
Beta strandi128 – 130Combined sources3
Beta strandi136 – 138Combined sources3
Beta strandi140 – 147Combined sources8
Beta strandi149 – 160Combined sources12
Beta strandi164 – 166Combined sources3
Beta strandi175 – 179Combined sources5
Turni183 – 185Combined sources3
Beta strandi186 – 188Combined sources3
Helixi190 – 193Combined sources4
Helixi197 – 199Combined sources3
Beta strandi202 – 207Combined sources6
Helixi211 – 220Combined sources10
Turni221 – 223Combined sources3
Beta strandi224 – 230Combined sources7
Beta strandi232 – 235Combined sources4
Helixi240 – 247Combined sources8
Beta strandi252 – 255Combined sources4
Beta strandi257 – 279Combined sources23
Turni280 – 282Combined sources3
Beta strandi285 – 289Combined sources5
Beta strandi291 – 295Combined sources5
Beta strandi299 – 302Combined sources4
Helixi304 – 306Combined sources3
Beta strandi332 – 334Combined sources3
Helixi336 – 339Combined sources4
Helixi346 – 366Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VDCX-ray2.50A43-375[»]
ProteinModelPortaliQ39243
SMRiQ39243
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ39243

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0404 Eukaryota
COG0492 LUCA
HOGENOMiHOG000072912
InParanoidiQ39243
KOiK00384
OMAiSYCTICD
OrthoDBiEOG09360E6S
PhylomeDBiQ39243

Family and domain databases

Gene3Di3.50.50.603 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR008255 Pyr_nucl-diS_OxRdtase_2_AS
IPR000103 Pyridine_nuc-diS_OxRdtase_2
IPR005982 Thioredox_Rdtase
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PRINTSiPR00469 PNDRDTASEII
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR01292 TRX_reduct, 1 hit
PROSITEiView protein in PROSITE
PS00573 PYRIDINE_REDOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCVSRLKCL ISKARSFARL GGESTLSQPP SLASAAFSSS AVMNGLETHN
60 70 80 90 100
TRLCIVGSGP AAHTAAIYAA RAELKPLLFE GWMANDIAPG GQLTTTTDVE
110 120 130 140 150
NFPGFPEGIL GVELTDKFRK QSERFGTTIF TETVTKVDFS SKPFKLFTDS
160 170 180 190 200
KAILADAVIL ATGAVAKRLS FVGSGEASGG FWNRGISACA VCDGAAPIFR
210 220 230 240 250
NKPLAVIGGG DSAMEEANFL TKYGSKVYII HRRDAFRASK IMQQRALSNP
260 270 280 290 300
KIDVIWNSSV VEAYGDGERD VLGGLKVKNV VTGDVSDLKV SGLFFAIGHE
310 320 330 340 350
PATKFLDGGV ELDSDGYVVT KPGTTQTSVP GVFAAGDVQD KKYRQAITAA
360 370
GTGCMAALDA EHYLQEIGSQ QGKSD
Length:375
Mass (Da):39,626
Last modified:June 15, 2010 - v3
Checksum:iF86B7FF70340C952
GO

Sequence cautioni

The sequence AAO42318 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA80656 differs from that shown. Sequencing errors.Curated
The sequence CAB54874 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB80262 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255I → F in AAO42318 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23109 mRNA Translation: CAA80656.1 Sequence problems.
AL117188 Genomic DNA Translation: CAB54874.1 Different initiation.
AL161587 Genomic DNA Translation: CAB80262.1 Different initiation.
CP002687 Genomic DNA Translation: AEE86518.1
BT004322 mRNA Translation: AAO42318.1 Different initiation.
PIRiS44027
T41743
RefSeqiNP_195271.2, NM_119711.4
UniGeneiAt.3705
At.69236

Genome annotation databases

EnsemblPlantsiAT4G35460.1; AT4G35460.1; AT4G35460
GeneIDi829698
GrameneiAT4G35460.1; AT4G35460.1; AT4G35460
KEGGiath:AT4G35460

Similar proteinsi

Entry informationi

Entry nameiTRXB1_ARATH
AccessioniPrimary (citable) accession number: Q39243
Secondary accession number(s): Q84W20, Q9SVW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 15, 2010
Last modified: April 25, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome