Q39233 (SYP21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-21
Short name=AtSYP21

Alternative name(s):
PEP12 homolog
Short name=AtPEP12
aPEP12

Gene names

Name:	SYP21
Ordered Locus Names:	At5g16830
ORF Names:	F5E19_170

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function in the docking or fusion of transport vesicles with the prevacuolar membrane.
Subunit structure	Interacts with VTI11 and SYP51 to form a t-SNARE complex and with alpha-SNAP to form a 20S complex. Ref.7 Ref.8
Subcellular location	Prevacuolar compartment membrane; Single-pass type IV membrane protein Ref.6.
Tissue specificity	A high level expression is seen in the roots while a low level expression is seen in the leaves.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to vacuole transport Traceable author statement Ref.7. Source: TAIR late endosome to vacuole transport Inferred from mutant phenotype PubMed 16935987. Source: TAIR protein targeting to vacuole Inferred from direct assay PubMed 17662029. Source: TAIR
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW late endosome Inferred from direct assay PubMed 16935987. Source: TAIR late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from direct assay PubMed 16935987. Source: TAIR trans-Golgi network transport vesicle Inferred from direct assay Ref.6. Source: TAIR
Molecular_function	SNAP receptor activity Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VTI11	Q9SEL6	3	EBI-2352544,EBI-1162795

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Syntaxin-21		PRO_0000210253
Regions
	Topological domain	1 – 258	258	Cytoplasmic Potential
	Transmembrane	259 – 279	21	Helical; Anchor for type IV membrane protein; Potential
	Domain	186 – 248	63	t-SNARE coiled-coil homology
	Coiled coil	65 – 94	30	Potential
Experimental info
	Sequence conflict	14	1	A → T in AAM66128. Ref.5
	Sequence conflict	263	1	I → T in AAM66128. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q39233 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EDAB4B07CC377CE3	FASTA	279	31,061
	10 20 30 40 50 60 MSFQDLEAGT RSPAPNRFTG GRQQRPSSRG DPSQEVAAGI FRISTAVNSF FRLVNSIGTP 70 80 90 100 110 120 KDTLELRDKL QKTRLQISEL VKNTSAKLKE ASEADLHGSA SQIKKIADAK LAKDFQSVLK 130 140 150 160 170 180 EFQKAQRLAA EREITYTPVV TKEIPTSYNA PELDTESLRI SQQQALLLQS RRQEVVFLDN 190 200 210 220 230 240 EITFNEAIIE EREQGIREIE DQIRDVNGMF KDLALMVNHQ GNIVDDISSN LDNSHAATTQ 250 260 270 ATVQLRKAAK TQRSNSSLTC LLILIFGIVL LIVIIVVLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant." Bassham D.C., Gal S., da Silva Conceicao A., Raikhel N.V. Proc. Natl. Acad. Sci. U.S.A. 92:7262-7266(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. RLD.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The syntaxin homolog AtPEP12p resides on a late post-Golgi compartment in plants." da Silva Conceicao A., Marty-Mazars D., Bassham D.C., Sanderfoot A.A., Marty F., Raikhel N.V. Plant Cell 9:571-582(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"The pre-vacuolar t-SNARE AtPEP12p forms a 20S complex that dissociates in the presence of ATP." Bassham D.C., Raikhel N.V. Plant J. 19:599-603(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ALPHA-SNAP.
[8]	"Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell." Sanderfoot A.A., Kovaleva V., Bassham D.C., Raikhel N.V. Mol. Biol. Cell 12:3733-3743(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VTI11 AND SYP51.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L41651 mRNA. Translation: AAA87296.1. AL391147 Genomic DNA. Translation: CAC01847.1. CP002688 Genomic DNA. Translation: AED92345.1. AF411797 mRNA. Translation: AAL06486.1. AY088599 mRNA. Translation: AAM66128.1.
IPI	IPI00545765.
PIR	T51515.
RefSeq	NP_197185.1. NM_121689.3.
UniGene	At.1639. At.5364.
3D structure databases
ProteinModelPortal	Q39233.
SMR	Q39233. Positions 191-248.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39233. 3 interactions.
Proteomic databases
PaxDb	Q39233.
PRIDE	Q39233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G16830.1; AT5G16830.1; AT5G16830.
GeneID	831546.
KEGG	ath:AT5G16830.
Organism-specific databases
GeneFarm	2178. 383.
TAIR	At5g16830.
Phylogenomic databases
eggNOG	NOG283133.
HOGENOM	HOG000188453.
InParanoid	Q39233.
KO	K08488.
OMA	REQGIRE.
PhylomeDB	Q39233.
ProtClustDB	CLSN2686175.
Gene expression databases
ArrayExpress	Q39233.
Genevestigator	Q39233.
GermOnline	AT5G16830. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
Pfam	PF05739. SNARE. 1 hit. PF00804. Syntaxin. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP21_ARATH

Accession

Primary (citable) accession number: Q39233

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program