Alternative oxidase 1a, mitochondrial precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q39219 (AOX1A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alternative oxidase 1a, mitochondrial
EC=1.-.-.-

Gene names

Name:	AOX1A
Synonyms:	AOX1
Ordered Locus Names:	At3g22370
ORF Names:	MCB17.11

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures By similarity.
Cofactor	Binds 2 iron ions per subunit.
Subunit structure	Homodimer; disulfide-linked Probable.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Probable. Note: Mitochondrial, possibly in the inner surface of the inner mitochondrial membrane. Ref.10
Sequence similarities	Belongs to the alternative oxidase family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular respiration Inferred from direct assay. Source: TAIR electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW mitochondria-nucleus signaling pathway Inferred from expression pattern. Source: TAIR respiratory gaseous exchange Inferred from electronic annotation. Source: InterPro response to cold Inferred from mutant phenotype. Source: TAIR transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alternative oxidase activity Inferred from direct assay. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 62

Mitochondrion Potential

Chain

63 – 354

292

Alternative oxidase 1a, mitochondrial

PRO_0000001731

Regions

Transmembrane

179 – 199

Helical; Potential

Transmembrane

241 – 261

Helical; Potential

Sites

Metal binding

183

Iron Potential

Metal binding

222

Iron Potential

Metal binding

225

Iron Potential

Metal binding

274

Iron Potential

Metal binding

324

Iron Potential

Metal binding

327

Iron Potential

Amino acid modifications

Disulfide bond

127

Interchain Potential

Experimental info

Sequence conflict

50 – 51

IW → MD in AAA32870. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q39219 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 6DA19881E8BC9382
Show »

FASTA

354

39,980

        10         20         30         40         50         60 
MMITRGGAKA AKSLLVAAGP RLFSTVRTVS SHEALSASHI LKPGVTSAWI WTRAPTIGGM 

        70         80         90        100        110        120 
RFASTITLGE KTPMKEEDAN QKKTENESTG GDAAGGNNKG DKGIASYWGV EPNKITKEDG 

       130        140        150        160        170        180 
SEWKWNCFRP WETYKADITI DLKKHHVPTT FLDRIAYWTV KSLRWPTDLF FQRRYGCRAM 

       190        200        210        220        230        240 
MLETVAAVPG MVGGMLLHCK SLRRFEQSGG WIKALLEEAE NERMHLMTFM EVAKPKWYER 

       250        260        270        280        290        300 
ALVITVQGVF FNAYFLGYLI SPKFAHRMVG YLEEEAIHSY TEFLKELDKG NIENVPAPAI 

       310        320        330        340        350 
AIDYWRLPAD ATLRDVVMVV RADEAHHRDV NHFASDIHYQ GRELKEAPAP IGYH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the gene family for alternative oxidase from Arabidopsis thaliana." Saisho D., Nambara E., Naito S., Tsutsumi N., Hirai A., Nakazono M. Plant Mol. Biol. 35:585-596(1997) [PubMed: 9349280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Leaf and Stem.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis alternative oxidase sustains Escherichia coli respiration." Kumar A.M., Soell D. Proc. Natl. Acad. Sci. U.S.A. 89:10842-10846(1992) [PubMed: 1438286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-354.
[6]	Johnson Potter F., Wiskich J.T. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-354.
[7]	"A revised model of the active site of alternative oxidase." Andersson M.E., Nordlund P. FEBS Lett. 449:17-22(1999) [PubMed: 10225419] [Abstract] Cited for: IRON-BINDING SITES.
[8]	"New insight into the structure and function of the alternative oxidase." Berthold D.A., Andersson M.E., Nordlund P. Biochim. Biophys. Acta 1460:241-254(2000) [PubMed: 11106766] [Abstract] Cited for: IRON-BINDING SITES.
[9]	"EPR studies of the mitochondrial alternative oxidase. Evidence for a diiron carboxylate center." Berthold D.A., Voevodskaya N., Stenmark P., Graslund A., Nordlund P. J. Biol. Chem. 277:43608-43614(2002) [PubMed: 12215444] [Abstract] Cited for: EPR SPECTROSCOPY.
[10]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89875 Genomic DNA. Translation: BAA22625.1. AB022215 Genomic DNA. Translation: BAB01775.1. CP002686 Genomic DNA. Translation: AEE76627.1. AF370166 mRNA. Translation: AAK43981.1. AY059128 mRNA. Translation: AAL15234.1. M96417 mRNA. Translation: AAA32870.1. U85244 Genomic DNA. Translation: AAB49302.1.
IPI	IPI00546366.
PIR	A46364.
RefSeq	NP_188876.1. NM_113135.3.
UniGene	At.23475.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q39219.
Proteomic databases
PRIDE	Q39219.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G22370.1; AT3G22370.1; AT3G22370.
GeneID	821806.
GenomeReviews	Gene locus AT3G22370 in contig BA000014_GR.
KEGG	ath:AT3G22370.
NMPDR	fig\|3702.1.peg.14480.
Organism-specific databases
GeneFarm	1749. 131.
TAIR	At3g22370.
Phylogenomic databases
eggNOG	euNOG04325.
HOGENOM	HBG597538.
InParanoid	Q39219.
OMA	KEEDANQ.
PhylomeDB	Q39219.
ProtClustDB	PLN02478.
Enzyme and pathway databases
BioCyc	MetaCyc:AT3G22370-MONOMER.
Gene expression databases
ArrayExpress	Q39219.
Genevestigator	Q39219.
GermOnline	AT3G22370. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002680. AOX. [Graphical view]
KO	K00540.
Pfam	PF01786. AOX. 1 hit. [Graphical view]
PIRSF	PIRSF005229. AOX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AOX1A_ARATH

Accession

Primary (citable) accession number: Q39219
Secondary accession number(s): O23914, P93734

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 15, 1998
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents