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Protein

Serine acetyltransferase 3, mitochondrial

Gene

SAT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.

Kineticsi

  1. KM=1.68 mM for L-Ser (at pH 8 and 37 degrees Celsius)1 Publication
  2. KM=0.02 mM for acetyl-CoA (at pH 8 and 37 degrees Celsius)1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
    2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    GO - Molecular functioni

    • serine O-acetyltransferase activity Source: TAIR
    • zinc ion binding Source: TAIR

    GO - Biological processi

    • cellular amino acid biosynthetic process Source: UniProtKB-KW
    • cysteine biosynthetic process Source: TAIR
    • cysteine biosynthetic process from serine Source: InterPro
    • response to cadmium ion Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT3G13110-MONOMER.
    MetaCyc:AT3G13110-MONOMER.
    BRENDAi2.3.1.30. 399.
    UniPathwayiUPA00136; UER00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine acetyltransferase 3, mitochondrial (EC:2.3.1.30)
    Short name:
    AtSAT-3
    Short name:
    AtSERAT2;2
    Short name:
    SAT-m
    Gene namesi
    Name:SAT3
    Synonyms:SAT1, SATA
    Ordered Locus Names:At3g13110
    ORF Names:MJG19.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G13110.

    Subcellular locationi

    • Mitochondrion 1 Publication

    GO - Cellular componenti

    • mitochondrion Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000045777? – 391Serine acetyltransferase 3, mitochondrial
    Transit peptidei1 – ?MitochondrionSequence analysis

    Proteomic databases

    PaxDbiQ39218.
    PRIDEiQ39218.

    PTM databases

    iPTMnetiQ39218.

    Expressioni

    Tissue specificityi

    Ubiquitous with higher levels in leaves and siliques. Localized in vascular tissues, particularly in phloem.3 Publications

    Inductioni

    By light, cadmium (Cd), and slightly by sulfur deficiency.3 Publications

    Gene expression databases

    GenevisibleiQ39218. AT.

    Interactioni

    Subunit structurei

    Homomultimer (By similarity). Interacts with OASC. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    OASCQ437254EBI-1633440,EBI-1633616

    Protein-protein interaction databases

    BioGridi5833. 3 interactors.
    DIPiDIP-40499N.
    IntActiQ39218. 1 interactor.
    STRINGi3702.AT3G13110.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39218.
    SMRiQ39218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiENOG410INAF. Eukaryota.
    COG1045. LUCA.
    InParanoidiQ39218.
    KOiK00640.
    OMAiYHASIVS.
    OrthoDBiEOG09360EFX.
    PhylomeDBiQ39218.

    Family and domain databases

    InterProiIPR001451. Hexapep.
    IPR018357. Hexapep_transf_CS.
    IPR010493. Ser_AcTrfase_N.
    IPR005881. Ser_O-AcTrfase.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 1 hit.
    PF06426. SATase_N. 1 hit.
    [Graphical view]
    SMARTiSM00971. SATase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    TIGRFAMsiTIGR01172. cysE. 1 hit.
    PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q39218-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLPVTSRRHF TMSLYMLRSS SPHINHHSFL LPSFVSSKFK HHTLSPPPSP
    60 70 80 90 100
    PPPPPMAACI DTCRTGKPQI SPRDSSKHHD DESGFRYMNY FRYPDRSSFN
    110 120 130 140 150
    GTQTKTLHTR PLLEDLDRDA EVDDVWAKIR EEAKSDIAKE PIVSAYYHAS
    160 170 180 190 200
    IVSQRSLEAA LANTLSVKLS NLNLPSNTLF DLFSGVLQGN PDIVESVKLD
    210 220 230 240 250
    LLAVKERDPA CISYVHCFLH FKGFLACQAH RIAHELWTQD RKILALLIQN
    260 270 280 290 300
    RVSEAFAVDF HPGAKIGTGI LLDHATAIVI GETAVVGNNV SILHNVTLGG
    310 320 330 340 350
    TGKQCGDRHP KIGDGVLIGA GTCILGNITI GEGAKIGAGS VVLKDVPPRT
    360 370 380 390
    TAVGNPARLL GGKDNPKTHD KIPGLTMDQT SHISEWSDYV I
    Length:391
    Mass (Da):42,721
    Last modified:February 10, 2009 - v3
    Checksum:iA3ED09DEA53C10E2
    GO

    Sequence cautioni

    The sequence AAB02050 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA56913 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti186V → G in AAB02050 (Ref. 5) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U22964 mRNA. Translation: AAB07778.1.
    AP000375 Genomic DNA. Translation: BAB01402.1.
    CP002686 Genomic DNA. Translation: AEE75297.1.
    BT026471 mRNA. Translation: ABH04578.1.
    L78443 Genomic DNA. Translation: AAB02050.1. Different initiation.
    AK226230 mRNA. Translation: BAE98394.1.
    X80938 mRNA. Translation: CAA56913.1. Different initiation.
    X82888 mRNA. Translation: CAA58061.1.
    PIRiS68469.
    S69192.
    RefSeqiNP_187918.1. NM_112150.4.
    UniGeneiAt.8123.

    Genome annotation databases

    EnsemblPlantsiAT3G13110.1; AT3G13110.1; AT3G13110.
    GeneIDi820499.
    GrameneiAT3G13110.1; AT3G13110.1; AT3G13110.
    KEGGiath:AT3G13110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U22964 mRNA. Translation: AAB07778.1.
    AP000375 Genomic DNA. Translation: BAB01402.1.
    CP002686 Genomic DNA. Translation: AEE75297.1.
    BT026471 mRNA. Translation: ABH04578.1.
    L78443 Genomic DNA. Translation: AAB02050.1. Different initiation.
    AK226230 mRNA. Translation: BAE98394.1.
    X80938 mRNA. Translation: CAA56913.1. Different initiation.
    X82888 mRNA. Translation: CAA58061.1.
    PIRiS68469.
    S69192.
    RefSeqiNP_187918.1. NM_112150.4.
    UniGeneiAt.8123.

    3D structure databases

    ProteinModelPortaliQ39218.
    SMRiQ39218.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi5833. 3 interactors.
    DIPiDIP-40499N.
    IntActiQ39218. 1 interactor.
    STRINGi3702.AT3G13110.1.

    PTM databases

    iPTMnetiQ39218.

    Proteomic databases

    PaxDbiQ39218.
    PRIDEiQ39218.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G13110.1; AT3G13110.1; AT3G13110.
    GeneIDi820499.
    GrameneiAT3G13110.1; AT3G13110.1; AT3G13110.
    KEGGiath:AT3G13110.

    Organism-specific databases

    TAIRiAT3G13110.

    Phylogenomic databases

    eggNOGiENOG410INAF. Eukaryota.
    COG1045. LUCA.
    InParanoidiQ39218.
    KOiK00640.
    OMAiYHASIVS.
    OrthoDBiEOG09360EFX.
    PhylomeDBiQ39218.

    Enzyme and pathway databases

    UniPathwayiUPA00136; UER00199.
    BioCyciARA:AT3G13110-MONOMER.
    MetaCyc:AT3G13110-MONOMER.
    BRENDAi2.3.1.30. 399.

    Miscellaneous databases

    PROiQ39218.

    Gene expression databases

    GenevisibleiQ39218. AT.

    Family and domain databases

    InterProiIPR001451. Hexapep.
    IPR018357. Hexapep_transf_CS.
    IPR010493. Ser_AcTrfase_N.
    IPR005881. Ser_O-AcTrfase.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 1 hit.
    PF06426. SATase_N. 1 hit.
    [Graphical view]
    SMARTiSM00971. SATase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    TIGRFAMsiTIGR01172. cysE. 1 hit.
    PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSAT3_ARATH
    AccessioniPrimary (citable) accession number: Q39218
    Secondary accession number(s): Q0V7U9
    , Q0WWV4, Q42532, Q43739, Q43740, Q7DLZ8, Q7DM01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: February 10, 2009
    Last modified: November 30, 2016
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.