Reviewed, UniProtKB/Swiss-Prot Q39218 (SAT3_ARATH)

Names and origin

Protein names

Recommended name:
    Serine acetyltransferase 3, mitochondrial
      Short name=AtSAT-3
      Short name=SAT-m
      Short name=AtSERAT2;2
    EC=2.3.1.30

Gene names

Name:	SAT3
Synonyms:	SAT1, SATA
Ordered Locus Names:	At3g13110
ORF Names:	MJG19.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
Subunit structure	Homomultimer By similarity.
Subcellular location	Mitochondrion. Ref.7
Tissue specificity	Ubiquitous with higher levels in leaves and siliques. Localized in vascular tissues, particularly in phloem. Ref.6 Ref.8 Ref.9
Induction	By light, cadmium (Cd), and slightly by sulfur deficiency. Ref.6 Ref.8 Ref.9
Sequence similarities	Belongs to the transferase hexapeptide repeat family.
Biophysicochemical properties	Kinetic parameters: KM=1.68 mM for L-Ser (at pH 8 and 37 degrees Celsius) KM=0.02 mM for acetyl-CoA (at pH 8 and 37 degrees Celsius)

Ontologies

Keywords
Biological process	Amino-acid biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	mitochondrion Ref.7 Inferred from direct assay. Source: TAIR
Molecular function	serine O-acetyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 391		Serine acetyltransferase 3, mitochondrial		PRO_0000045777
Experimental info
	Sequence conflict	186	1	V → G in AAB02050. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q39218-1 [UniParc]. Last modified February 10, 2009. Version 3. Checksum: A3ED09DEA53C10E2	FASTA	391	42,721
	10 20 30 40 50 60 MLPVTSRRHF TMSLYMLRSS SPHINHHSFL LPSFVSSKFK HHTLSPPPSP PPPPPMAACI 70 80 90 100 110 120 DTCRTGKPQI SPRDSSKHHD DESGFRYMNY FRYPDRSSFN GTQTKTLHTR PLLEDLDRDA 130 140 150 160 170 180 EVDDVWAKIR EEAKSDIAKE PIVSAYYHAS IVSQRSLEAA LANTLSVKLS NLNLPSNTLF 190 200 210 220 230 240 DLFSGVLQGN PDIVESVKLD LLAVKERDPA CISYVHCFLH FKGFLACQAH RIAHELWTQD 250 260 270 280 290 300 RKILALLIQN RVSEAFAVDF HPGAKIGTGI LLDHATAIVI GETAVVGNNV SILHNVTLGG 310 320 330 340 350 360 TGKQCGDRHP KIGDGVLIGA GTCILGNITI GEGAKIGAGS VVLKDVPPRT TAVGNPARLL 370 380 390 GGKDNPKTHD KIPGLTMDQT SHISEWSDYV I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of an Arabidopsis thaliana cDNA clone encoding an organellar isoform of serine acetyltransferase." Roberts M.A., Wray J.L. Plant Mol. Biol. 30:1041-1049(1996) [PubMed: 8639741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Gene sequence of serine acetyltranferase 1 from A. thaliana." Ruffet M.-L., Lebrun M., Droux M., Douce R. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-391.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-391. Strain: cv. Columbia.
[6]	"Cysteine biosynthesis in plants: isolation and functional identification of a cDNA encoding a serine acetyltransferase from Arabidopsis thaliana." Bogdanova N., Bork C., Hell R. FEBS Lett. 358:43-47(1995) [PubMed: 7821427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-391 AND 80-391, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia. Tissue: Leaf.
[7]	"Isoform-dependent differences in feedback regulation and subcellular localization of serine acetyltransferase involved in cysteine biosynthesis from Arabidopsis thaliana." Noji M., Inoue K., Kimura N., Gouda A., Saito K. J. Biol. Chem. 273:32739-32745(1998) [PubMed: 9830017] [Abstract] Cited for: SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
[8]	"The serine acetyltransferase gene family in Arabidopsis thaliana and the regulation of its expression by cadmium." Howarth J.R., Dominguez-Solis J.R., Gutierrez-Alcala G., Wray J.L., Romero L.C., Gotor C. Plant Mol. Biol. 51:589-598(2003) [PubMed: 12650624] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[9]	"Characterization and expression analysis of a serine acetyltransferase gene family involved in a key step of the sulfur assimilation pathway in Arabidopsis." Kawashima C.G., Berkowitz O., Hell R., Noji M., Saito K. Plant Physiol. 137:220-230(2005) [PubMed: 15579666] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U22964 mRNA. Translation: AAB07778.1. AP000375 Genomic DNA. Translation: BAB01402.1. BT026471 mRNA. Translation: ABH04578.1. L78443 Genomic DNA. Translation: AAB02050.1. Different initiation. AK226230 mRNA. Translation: BAE98394.1. X80938 mRNA. Translation: CAA56913.1. Different initiation. X82888 mRNA. Translation: CAA58061.1.
IPI	IPI00546531.
PIR	S68469. S69192.
RefSeq	NP_187918.1.
UniGene	At.8123
3D structure databases
HSSP	HSSP built from PDB template 1MR9 based on UniProtKB P50870.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39218. 5 interactions.
STRING	Q39218.
Proteomic databases
PRIDE	Q39218.
Genome annotation databases
GeneID	820499.
GenomeReviews	Gene locus AT3G13110 in contig BA000014_GR.
KEGG	ath:AT3G13110.
NMPDR	fig|3702.1.peg.13402.
Organism-specific databases
GeneFarm	5179. 495.
TAIR	At3g13110.
Enzyme and pathway databases
BRENDA	2.3.1.30. 302.
Gene expression databases
Genevestigator	Q39218.
GermOnline	AT3G13110. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010493. Ser_AcTrfase_N. IPR005881. Ser_O-AcTrfase. [Graphical view]
Pfam	PF00132. Hexapep. 3 hits. PF06426. SATase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01172. cysE. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT3_ARATH

Accession

Primary (citable) accession number: Q39218
Secondary accession number(s): Q0V7U9 , Q0WWV4, Q42532, Q43739, Q43740, Q7DLZ8, Q7DM01

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)