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Protein

Serine acetyltransferase 3, mitochondrial

Gene

SAT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.

Kineticsi

  1. KM=1.68 mM for L-Ser (at pH 8 and 37 degrees Celsius)1 Publication
  2. KM=0.02 mM for acetyl-CoA (at pH 8 and 37 degrees Celsius)1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
    2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    GO - Molecular functioni

    • serine O-acetyltransferase activity Source: TAIR
    • zinc ion binding Source: TAIR

    GO - Biological processi

    • cysteine biosynthetic process Source: TAIR
    • cysteine biosynthetic process from serine Source: InterPro
    • response to cadmium ion Source: TAIR

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processAmino-acid biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT3G13110-MONOMER
    MetaCyc:AT3G13110-MONOMER
    BRENDAi2.3.1.30 399
    UniPathwayiUPA00136; UER00199

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine acetyltransferase 3, mitochondrial (EC:2.3.1.30)
    Short name:
    AtSAT-3
    Short name:
    AtSERAT2;2
    Short name:
    SAT-m
    Gene namesi
    Name:SAT3
    Synonyms:SAT1, SATA
    Ordered Locus Names:At3g13110
    ORF Names:MJG19.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G13110
    TAIRilocus:2089974 AT3G13110

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000045777? – 391Serine acetyltransferase 3, mitochondrial
    Transit peptidei1 – ?MitochondrionSequence analysis

    Proteomic databases

    PaxDbiQ39218
    PRIDEiQ39218

    PTM databases

    iPTMnetiQ39218

    Expressioni

    Tissue specificityi

    Ubiquitous with higher levels in leaves and siliques. Localized in vascular tissues, particularly in phloem.3 Publications

    Inductioni

    By light, cadmium (Cd), and slightly by sulfur deficiency.3 Publications

    Gene expression databases

    ExpressionAtlasiQ39218 baseline and differential
    GenevisibleiQ39218 AT

    Interactioni

    Subunit structurei

    Homomultimer (By similarity). Interacts with OASC. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    OASCQ437256EBI-1633440,EBI-1633616

    Protein-protein interaction databases

    BioGridi5833, 3 interactors
    DIPiDIP-40499N
    IntActiQ39218, 1 interactor
    STRINGi3702.AT3G13110.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ39218
    SMRiQ39218
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiENOG410INAF Eukaryota
    COG1045 LUCA
    InParanoidiQ39218
    KOiK00640
    OMAiDQTSHIS
    OrthoDBiEOG09360EFX
    PhylomeDBiQ39218

    Family and domain databases

    InterProiView protein in InterPro
    IPR001451 Hexapep
    IPR018357 Hexapep_transf_CS
    IPR010493 Ser_AcTrfase_N
    IPR005881 Ser_O-AcTrfase
    IPR011004 Trimer_LpxA-like_sf
    PfamiView protein in Pfam
    PF00132 Hexapep, 1 hit
    PF06426 SATase_N, 1 hit
    SMARTiView protein in SMART
    SM00971 SATase_N, 1 hit
    SUPFAMiSSF51161 SSF51161, 1 hit
    TIGRFAMsiTIGR01172 cysE, 1 hit
    PROSITEiView protein in PROSITE
    PS00101 HEXAPEP_TRANSFERASES, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q39218-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLPVTSRRHF TMSLYMLRSS SPHINHHSFL LPSFVSSKFK HHTLSPPPSP
    60 70 80 90 100
    PPPPPMAACI DTCRTGKPQI SPRDSSKHHD DESGFRYMNY FRYPDRSSFN
    110 120 130 140 150
    GTQTKTLHTR PLLEDLDRDA EVDDVWAKIR EEAKSDIAKE PIVSAYYHAS
    160 170 180 190 200
    IVSQRSLEAA LANTLSVKLS NLNLPSNTLF DLFSGVLQGN PDIVESVKLD
    210 220 230 240 250
    LLAVKERDPA CISYVHCFLH FKGFLACQAH RIAHELWTQD RKILALLIQN
    260 270 280 290 300
    RVSEAFAVDF HPGAKIGTGI LLDHATAIVI GETAVVGNNV SILHNVTLGG
    310 320 330 340 350
    TGKQCGDRHP KIGDGVLIGA GTCILGNITI GEGAKIGAGS VVLKDVPPRT
    360 370 380 390
    TAVGNPARLL GGKDNPKTHD KIPGLTMDQT SHISEWSDYV I
    Length:391
    Mass (Da):42,721
    Last modified:February 10, 2009 - v3
    Checksum:iA3ED09DEA53C10E2
    GO

    Sequence cautioni

    The sequence AAB02050 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA56913 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti186V → G in AAB02050 (Ref. 5) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U22964 mRNA Translation: AAB07778.1
    AP000375 Genomic DNA Translation: BAB01402.1
    CP002686 Genomic DNA Translation: AEE75297.1
    BT026471 mRNA Translation: ABH04578.1
    L78443 Genomic DNA Translation: AAB02050.1 Different initiation.
    AK226230 mRNA Translation: BAE98394.1
    X80938 mRNA Translation: CAA56913.1 Different initiation.
    X82888 mRNA Translation: CAA58061.1
    PIRiS68469
    S69192
    RefSeqiNP_187918.1, NM_112150.4
    UniGeneiAt.8123

    Genome annotation databases

    EnsemblPlantsiAT3G13110.1; AT3G13110.1; AT3G13110
    GeneIDi820499
    GrameneiAT3G13110.1; AT3G13110.1; AT3G13110
    KEGGiath:AT3G13110

    Similar proteinsi

    Entry informationi

    Entry nameiSAT3_ARATH
    AccessioniPrimary (citable) accession number: Q39218
    Secondary accession number(s): Q0V7U9
    , Q0WWV4, Q42532, Q43739, Q43740, Q7DLZ8, Q7DM01
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: February 10, 2009
    Last modified: April 25, 2018
    This is version 123 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health