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Protein

Disease resistance protein RPM1

Gene

RPM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Disease resistance (R) protein that specifically recognizes the AvrRpm1 type III effector avirulence protein from Pseudomonas syringae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Acts via its interaction with RIN4, and probably triggers the plant resistance when RIN4 is phosphorylated by AvrRpm1. It is then degraded at the onset of the hypersensitive response.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi200 – 207ATPSequence analysis8

GO - Molecular functioni

  • ADP binding Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • nucleotide binding Source: TAIR

GO - Biological processi

  • plant-type hypersensitive response Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Hypersensitive response, Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Disease resistance protein RPM1
Alternative name(s):
Resistance to Pseudomonas syringae protein 3
Gene namesi
Name:RPM1
Synonyms:RPS3
Ordered Locus Names:At3g07040
ORF Names:F17A9.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G07040.

Subcellular locationi

GO - Cellular componenti

  • endomembrane system Source: UniProtKB-SubCell
  • extrinsic component of plasma membrane Source: CACAO
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43S → F: Loss of function. 1 Publication1
Mutagenesisi105P → S: Loss of function. 1 Publication1
Mutagenesisi174G → R: Loss of function. 1 Publication1
Mutagenesisi186L → F: Loss of function. 1 Publication1
Mutagenesisi203G → D or S: Loss of function. 1 Publication1
Mutagenesisi205G → R or E: Loss of function. 1 Publication1
Mutagenesisi211A → V: Loss of function. 1 Publication1
Mutagenesisi219V → M: Loss of function. 1 Publication1
Mutagenesisi247E → K: Loss of function. 1 Publication1
Mutagenesisi269V → M: Loss of function. 1 Publication1
Mutagenesisi272L → F: Loss of function. 1 Publication1
Mutagenesisi290T → I: Loss of function. 1 Publication1
Mutagenesisi301L → F in rps3-2; loss of function. 1 Publication1
Mutagenesisi307G → E: Loss of function. 1 Publication1
Mutagenesisi313T → I: Loss of function. 1 Publication1
Mutagenesisi325G → E: Loss of function. 1 Publication1
Mutagenesisi340E → K: Loss of function. 1 Publication1
Mutagenesisi341A → V: Loss of function. 1
Mutagenesisi344L → F: Loss of function. 1 Publication1
Mutagenesisi379A → V: Loss of function. 1 Publication1
Mutagenesisi384G → R: Loss of function. 1 Publication1
Mutagenesisi395E → K: Loss of function. 1 Publication1
Mutagenesisi439S → F: Loss of function. 1 Publication1
Mutagenesisi442P → L: Loss of function. 1 Publication1
Mutagenesisi464P → L: Loss of function. 1 Publication1
Mutagenesisi467G → R: Loss of function. 1 Publication1
Mutagenesisi471E → K: Loss of function. 1 Publication1
Mutagenesisi474A → T: Loss of function. 1 Publication1
Mutagenesisi494P → L: Loss of function. 1 Publication1
Mutagenesisi497R → Q: Loss of function. 1 Publication1
Mutagenesisi498P → L or S: Loss of function. 1 Publication1
Mutagenesisi515S → F: Loss of function. 1 Publication1
Mutagenesisi620P → S or L: Loss of function. 1 Publication1
Mutagenesisi738S → F: Loss of function. 1 Publication1
Mutagenesisi766G → E in rps3-4; loss of function. 1 Publication1
Mutagenesisi778L → F: Loss of function. 1 Publication1
Mutagenesisi781L → F: Loss of function. 1 Publication1
Mutagenesisi787R → H: Loss of function. 1 Publication1
Mutagenesisi812N → I: Loss of function. 1 Publication1
Mutagenesisi852L → F: Loss of function. 1 Publication1
Mutagenesisi924L → F: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002127171 – 926Disease resistance protein RPM1Add BLAST926

Proteomic databases

PaxDbiQ39214.

PTM databases

iPTMnetiQ39214.

Expressioni

Gene expression databases

GenevisibleiQ39214. AT.

Interactioni

Subunit structurei

Interacts directly with RIN4 via its N-terminal region. Interacts (via N-terminus) with RIN2 and RIN3 (via C-terminus). Interacts with TIP49A, a protein known to interact with the TATA binding protein complex (TBP) (PubMed:11955429, PubMed:12062092, PubMed:16212605). Binds to MORC1/CRT1 (PubMed:20332379).4 Publications

Protein-protein interaction databases

BioGridi5224. 7 interactors.
MINTiMINT-8060368.
STRINGi3702.AT3G07040.1.

Structurei

3D structure databases

ProteinModelPortaliQ39214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 467NB-ARCAdd BLAST315
Repeati560 – 581LRR 1Add BLAST22
Repeati582 – 603LRR 2Add BLAST22
Repeati605 – 626LRR 3Add BLAST22
Repeati628 – 650LRR 4Add BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 45Leucine-zipperAdd BLAST36

Domaini

The LRR repeats probably act as specificity determinant of pathogen recognition.

Sequence similaritiesi

Belongs to the disease resistance NB-LRR family.Curated
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 NB-ARC domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4658. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000237754.
InParanoidiQ39214.
KOiK13457.
OMAiKEDEAWV.
OrthoDBiEOG093601TL.
PhylomeDBiQ39214.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00931. NB-ARC. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATVDFGI GRILSVLENE TLLLSGVHGE IDKMKKELLI MKSFLEDTHK
60 70 80 90 100
HGGNGSTTTT TQLFQTFVAN TRDLAYQIED ILDEFGYHIH GYRSCAKIWR
110 120 130 140 150
AFHFPRYMWA RHSIAQKLGM VNVMIQSISD SMKRYYHSEN YQAALLPPID
160 170 180 190 200
DGDAKWVNNI SESSLFFSEN SLVGIDAPKG KLIGRLLSPE PQRIVVAVVG
210 220 230 240 250
MGGSGKTTLS ANIFKSQSVR RHFESYAWVT ISKSYVIEDV FRTMIKEFYK
260 270 280 290 300
EADTQIPAEL YSLGYRELVE KLVEYLQSKR YIVVLDDVWT TGLWREISIA
310 320 330 340 350
LPDGIYGSRV MMTTRDMNVA SFPYGIGSTK HEIELLKEDE AWVLFSNKAF
360 370 380 390 400
PASLEQCRTQ NLEPIARKLV ERCQGLPLAI ASLGSMMSTK KFESEWKKVY
410 420 430 440 450
STLNWELNNN HELKIVRSIM FLSFNDLPYP LKRCFLYCSL FPVNYRMKRK
460 470 480 490 500
RLIRMWMAQR FVEPIRGVKA EEVADSYLNE LVYRNMLQVI LWNPFGRPKA
510 520 530 540 550
FKMHDVIWEI ALSVSKLERF CDVYNDDSDG DDAAETMENY GSRHLCIQKE
560 570 580 590 600
MTPDSIRATN LHSLLVCSSA KHKMELLPSL NLLRALDLED SSISKLPDCL
610 620 630 640 650
VTMFNLKYLN LSKTQVKELP KNFHKLVNLE TLNTKHSKIE ELPLGMWKLK
660 670 680 690 700
KLRYLITFRR NEGHDSNWNY VLGTRVVPKI WQLKDLQVMD CFNAEDELIK
710 720 730 740 750
NLGCMTQLTR ISLVMVRREH GRDLCDSLNK IKRIRFLSLT SIDEEEPLEI
760 770 780 790 800
DDLIATASIE KLFLAGKLER VPSWFNTLQN LTYLGLRGSQ LQENAILSIQ
810 820 830 840 850
TLPRLVWLSF YNAYMGPRLR FAQGFQNLKI LEIVQMKHLT EVVIEDGAMF
860 870 880 890 900
ELQKLYVRAC RGLEYVPRGI ENLINLQELH LIHVSNQLVE RIRGEGSVDR
910 920
SRVKHIPAIK HYFRTDNGSF YVSLSS
Length:926
Mass (Da):106,883
Last modified:November 1, 1996 - v1
Checksum:iE2B2D6613CB0FA0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87851 Genomic DNA. Translation: CAA61131.1.
AC016827 Genomic DNA. Translation: AAF27008.1.
CP002686 Genomic DNA. Translation: AEE74492.1.
PIRiA57072.
RefSeqiNP_187360.1. NM_111584.3.
UniGeneiAt.40415.

Genome annotation databases

EnsemblPlantsiAT3G07040.1; AT3G07040.1; AT3G07040.
GeneIDi819889.
GrameneiAT3G07040.1; AT3G07040.1; AT3G07040.
KEGGiath:AT3G07040.

Cross-referencesi

Web resourcesi

NIB-LRRS

Functional and comparative genomics of disease resistance gene homologs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87851 Genomic DNA. Translation: CAA61131.1.
AC016827 Genomic DNA. Translation: AAF27008.1.
CP002686 Genomic DNA. Translation: AEE74492.1.
PIRiA57072.
RefSeqiNP_187360.1. NM_111584.3.
UniGeneiAt.40415.

3D structure databases

ProteinModelPortaliQ39214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5224. 7 interactors.
MINTiMINT-8060368.
STRINGi3702.AT3G07040.1.

PTM databases

iPTMnetiQ39214.

Proteomic databases

PaxDbiQ39214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G07040.1; AT3G07040.1; AT3G07040.
GeneIDi819889.
GrameneiAT3G07040.1; AT3G07040.1; AT3G07040.
KEGGiath:AT3G07040.

Organism-specific databases

TAIRiAT3G07040.

Phylogenomic databases

eggNOGiKOG4658. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000237754.
InParanoidiQ39214.
KOiK13457.
OMAiKEDEAWV.
OrthoDBiEOG093601TL.
PhylomeDBiQ39214.

Miscellaneous databases

PROiQ39214.

Gene expression databases

GenevisibleiQ39214. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00931. NB-ARC. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPM1_ARATH
AccessioniPrimary (citable) accession number: Q39214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.