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Protein

Transcription factor MYC2

Gene

MYC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Common transcription factor of light, abscisic acid (ABA), and jasmonic acid (JA) signaling pathways. With MYC3 and MYC4, controls additively subsets of JA-dependent responses. In cooperation with MYB2 is involved in the regulation of ABA-inducible genes under drought stress conditions. Can form complexes with all known glucosinolate-related MYBs to regulate glucosinolate biosynthesis. Binds to the MYC recognition site (5'-CACATG-3'), and to the G-box (5'-CACNTG-3') and Z-box (5'-ATACGTGT-3') of promoters. Binds directly to the promoters of the transcription factors PLETHORA1 (PLT1) and PLT2 and represses their expression. Negative regulator of blue light-mediated photomorphogenic growth and blue- and far-red-light regulated gene expression. Activates multiple TIFY/JAZ promoters. Positive regulator of lateral root formation. Regulates sesquiterpene biosynthesis. Subjected to proteasome-dependent proteolysis. The presence of the destruction element (DE) involved in turnover is required for the function to regulate gene transcription.11 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • positive regulation of flavonoid biosynthetic process Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • regulation of defense response to insect Source: TAIR
  • regulation of sequence-specific DNA binding transcription factor activity Source: TAIR
  • regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to chitin Source: TAIR
  • response to desiccation Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Abscisic acid signaling pathway, Jasmonic acid signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MYC2
Short name:
AtMYC2
Alternative name(s):
Basic helix-loop-helix protein 6
Short name:
AtbHLH6
Short name:
bHLH 6
Protein JASMONATE INSENSITIVE 1
R-homologous Arabidopsis protein 1
Short name:
RAP-1
Transcription factor EN 38
Z-box binding factor 1 protein
bHLH transcription factor bHLH006
rd22BP1
Gene namesi
Name:MYC2
Synonyms:BHLH6, EN38, JAI1, JIN1, RAP1, RD22BP1, ZBF1
Ordered Locus Names:At1g32640
ORF Names:F6N18.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G32640.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Minor effect on jasmonic acid response and no effect on glucosinolate biosynthesis, but decreased abscisic acid sensitivity. Myc2 and myc3 double mutant has an increased insensitivity to jasmonic acid. Myc2 and myc4 double mutant has an increased insensitivity to jasmonic acid. Myc2, myc3 and myc4 triple mutant has no jasmonate-related defense response, is devoid of glucosinolates and is extremely susceptible to generalist herbivores.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi328 – 3281T → A: Jasmonate-insensitive phenotype and increased stability of the protein. 1 Publication
Mutagenesisi330 – 3301S → A: No effect. 1 Publication
Mutagenesisi334 – 3341S → A: No effect. 1 Publication
Mutagenesisi336 – 3361T → A: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Transcription factor MYC2PRO_0000127427Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei328 – 3281Phosphothreonine1 Publication

Post-translational modificationi

The phosphorylation at Thr-328 is increased by methyl jasmonate treatment, facilitates the proteolysis of the protein and is coupled to transcription activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39204.
PRIDEiQ39204.

PTM databases

iPTMnetiQ39204.

Expressioni

Tissue specificityi

Widely expressed in the whole plant with the highest expression in stem. Constitutively expressed in dark- and light-grown seedlings.2 Publications

Inductioni

Detected early after abscisic acid (ABA) treatment or after dehydration and high-salt stresses. Induced by UV treatment. Up-regulated by methyl jasmonate and herbivory.5 Publications

Gene expression databases

GenevisibleiQ39204. AT.

Interactioni

Subunit structurei

Homo- and heterodimer. Efficient DNA binding requires dimerization with another bHLH protein (By similarity). Interacts (via N-terminus) with RGA, (via TAD domain) with the mediator subunit MED25, with TIC, MYC3, AFPH2/NINJA and the JAZ repressors TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY6A/JAZ4, TIFY11A/JAZ5, TIFY11B/JAZ6, TIFY5A/JAZ8, TIFY7/JAZ9, TIFY9/JAZ10, TIFY3A/JAZ11 and TIFY3B/JAZ12. Interacts with JAZ13 (via jas motif) (PubMed:25846245). Interacts with MYB28, MYB29, MYB34, MYB51, MYB76, MYB122 and mybe MYB2.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIFY10AQ9LMA83EBI-1792336,EBI-1388539
TIFY10BQ9S7M22EBI-1792336,EBI-1792563
TIFY11AQ9LDU52EBI-1792336,EBI-2312095
TIFY11BQ9C9E32EBI-1792336,EBI-2312120
TIFY3AQ9M2462EBI-1792336,EBI-2312209
TIFY3BQ9C5K82EBI-1792336,EBI-2312231
TIFY5AQ8LBM22EBI-1792336,EBI-2312143
TIFY6BQ9LVI45EBI-1792336,EBI-1792431
TIFY7Q8W4J83EBI-1792336,EBI-1792583
TIFY9Q93ZM92EBI-1792336,EBI-2312172

Protein-protein interaction databases

BioGridi25392. 46 interactions.
DIPiDIP-40533N.
IntActiQ39204. 11 interactions.
STRINGi3702.AT1G32640.1.

Structurei

3D structure databases

ProteinModelPortaliQ39204.
SMRiQ39204. Positions 66-253, 451-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini448 – 49750bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 16068JAZ-interaction domainAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi434 – 4429Nuclear localization signalSequence analysis

Domaini

The transcriptional activation domain (TAD) (149-188) overlaps with the destruction element (DE) (154-165).1 Publication
The JAZ-interaction domain (JID) (93-160) is sufficient for interaction with MYB proteins and most of the TIFY/JAZ proteins (PubMed:21335373 and PubMed:23943862).
The C-terminal half contains an active nuclear localization signal (NLS).1 Publication

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGFU. Eukaryota.
ENOG410YCJJ. LUCA.
HOGENOMiHOG000238207.
InParanoidiQ39204.
KOiK13422.
OMAiTQSFPNG.
OrthoDBiEOG093607T9.
PhylomeDBiQ39204.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR025610. MYC/MYB_N.
[Graphical view]
PfamiPF14215. bHLH-MYC_N. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYRLQPTM NLWTTDDNAS MMEAFMSSSD ISTLWPPAST TTTTATTETT
60 70 80 90 100
PTPAMEIPAQ AGFNQETLQQ RLQALIEGTH EGWTYAIFWQ PSYDFSGASV
110 120 130 140 150
LGWGDGYYKG EEDKANPRRR SSSPPFSTPA DQEYRKKVLR ELNSLISGGV
160 170 180 190 200
APSDDAVDEE VTDTEWFFLV SMTQSFACGA GLAGKAFATG NAVWVSGSDQ
210 220 230 240 250
LSGSGCERAK QGGVFGMHTI ACIPSANGVV EVGSTEPIRQ SSDLINKVRI
260 270 280 290 300
LFNFDGGAGD LSGLNWNLDP DQGENDPSMW INDPIGTPGS NEPGNGAPSS
310 320 330 340 350
SSQLFSKSIQ FENGSSSTIT ENPNLDPTPS PVHSQTQNPK FNNTFSRELN
360 370 380 390 400
FSTSSSTLVK PRSGEILNFG DEGKRSSGNP DPSSYSGQTQ FENKRKRSMV
410 420 430 440 450
LNEDKVLSFG DKTAGESDHS DLEASVVKEV AVEKRPKKRG RKPANGREEP
460 470 480 490 500
LNHVEAERQR REKLNQRFYA LRAVVPNVSK MDKASLLGDA IAYINELKSK
510 520 530 540 550
VVKTESEKLQ IKNQLEEVKL ELAGRKASAS GGDMSSSCSS IKPVGMEIEV
560 570 580 590 600
KIIGWDAMIR VESSKRNHPA ARLMSALMDL ELEVNHASMS VVNDLMIQQA
610 620
TVKMGFRIYT QEQLRASLIS KIG
Length:623
Mass (Da):67,950
Last modified:August 22, 2003 - v2
Checksum:i4A8D4DE34C5928F6
GO

Sequence cautioni

The sequence BAA25078 differs from that shown. Reason: Frameshift at positions 353 and 360. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti258 – 2581A → D in BAA25078 (PubMed:9368419).Curated
Sequence conflicti526 – 5261K → R in AAL55713 (PubMed:11130712).Curated
Sequence conflicti529 – 5291A → P in BAA25078 (PubMed:9368419).Curated
Sequence conflicti612 – 6121E → D in BAA25078 (PubMed:9368419).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99548 mRNA. Translation: CAA67885.2.
AB000875 mRNA. Translation: BAA25078.1. Frameshift.
AJ843256 mRNA. Translation: CAH58735.1.
AC017118 Genomic DNA. Translation: AAF25980.1.
CP002684 Genomic DNA. Translation: AEE31513.1.
AY037203 mRNA. Translation: AAK59788.1.
BT003042 mRNA. Translation: AAO23607.1.
AF251691 mRNA. Translation: AAL55713.1.
PIRiT52293.
RefSeqiNP_174541.1. NM_102998.3.
UniGeneiAt.22648.

Genome annotation databases

EnsemblPlantsiAT1G32640.1; AT1G32640.1; AT1G32640.
GeneIDi840158.
GrameneiAT1G32640.1; AT1G32640.1; AT1G32640.
KEGGiath:AT1G32640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99548 mRNA. Translation: CAA67885.2.
AB000875 mRNA. Translation: BAA25078.1. Frameshift.
AJ843256 mRNA. Translation: CAH58735.1.
AC017118 Genomic DNA. Translation: AAF25980.1.
CP002684 Genomic DNA. Translation: AEE31513.1.
AY037203 mRNA. Translation: AAK59788.1.
BT003042 mRNA. Translation: AAO23607.1.
AF251691 mRNA. Translation: AAL55713.1.
PIRiT52293.
RefSeqiNP_174541.1. NM_102998.3.
UniGeneiAt.22648.

3D structure databases

ProteinModelPortaliQ39204.
SMRiQ39204. Positions 66-253, 451-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25392. 46 interactions.
DIPiDIP-40533N.
IntActiQ39204. 11 interactions.
STRINGi3702.AT1G32640.1.

PTM databases

iPTMnetiQ39204.

Proteomic databases

PaxDbiQ39204.
PRIDEiQ39204.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G32640.1; AT1G32640.1; AT1G32640.
GeneIDi840158.
GrameneiAT1G32640.1; AT1G32640.1; AT1G32640.
KEGGiath:AT1G32640.

Organism-specific databases

TAIRiAT1G32640.

Phylogenomic databases

eggNOGiENOG410IGFU. Eukaryota.
ENOG410YCJJ. LUCA.
HOGENOMiHOG000238207.
InParanoidiQ39204.
KOiK13422.
OMAiTQSFPNG.
OrthoDBiEOG093607T9.
PhylomeDBiQ39204.

Miscellaneous databases

PROiQ39204.

Gene expression databases

GenevisibleiQ39204. AT.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR025610. MYC/MYB_N.
[Graphical view]
PfamiPF14215. bHLH-MYC_N. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYC2_ARATH
AccessioniPrimary (citable) accession number: Q39204
Secondary accession number(s): O48644
, Q5K1L7, Q8W2F4, Q9LPJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: August 22, 2003
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.