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Protein

Probable aquaporin PIP1-4

Gene

PIP1.4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.By similarity

GO - Molecular functioni

  • water channel activity Source: TAIR

GO - Biological processi

  • ion transmembrane transport Source: GO_Central
  • response to water deprivation Source: TAIR
  • water transport Source: TAIR

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aquaporin PIP1-4
Alternative name(s):
Plasma membrane intrinsic protein 1-4
Short name:
AtPIP1;4
Transmembrane protein C
Short name:
TMP-C
Gene namesi
Name:PIP1.4
Synonyms:TMPC
Ordered Locus Names:At4g00430
ORF Names:A_IG005I10.2, F5I10.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G00430.
TAIRilocus:2126026. AT4G00430.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55CytoplasmicSequence analysisAdd BLAST55
Transmembranei56 – 76Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini77 – 92ExtracellularSequence analysisAdd BLAST16
Transmembranei93 – 113Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini114 – 133CytoplasmicSequence analysisAdd BLAST20
Transmembranei134 – 154Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini155 – 175ExtracellularSequence analysisAdd BLAST21
Transmembranei176 – 196Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini197 – 209CytoplasmicSequence analysisAdd BLAST13
Transmembranei210 – 230Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini231 – 257ExtracellularSequence analysisAdd BLAST27
Transmembranei258 – 278Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini279 – 287CytoplasmicSequence analysis9

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • membrane Source: TAIR
  • plasma membrane Source: TAIR

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000640491 – 287Probable aquaporin PIP1-4Add BLAST287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei285PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ39196.
PRIDEiQ39196.

PTM databases

iPTMnetiQ39196.
SwissPalmiQ39196.

Expressioni

Tissue specificityi

Predominantly expressed in roots and green siliques. Also expressed above ground and in flower buds.1 Publication

Gene expression databases

ExpressionAtlasiQ39196. baseline and differential.
GenevisibleiQ39196. AT.

Interactioni

Protein-protein interaction databases

BioGridi13247. 12 interactors.
IntActiQ39196. 5 interactors.
STRINGi3702.AT4G00430.1.

Structurei

3D structure databases

ProteinModelPortaliQ39196.
SMRiQ39196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi115 – 117NPA 13
Motifi236 – 238NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiQ39196.
KOiK09872.
OMAiELFGTFW.
OrthoDBiEOG09360H22.
PhylomeDBiQ39196.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiView protein in InterPro
IPR023271. Aquaporin-like.
IPR034294. Aquaporin_transptr.
IPR000425. MIP.
IPR022357. MIP_CS.
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiView protein in Pfam
PF00230. MIP. 1 hit.
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiView protein in PROSITE
PS00221. MIP. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q39196-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGKEEDVRV GANKFPERQP IGTSAQSTDK DYKEPPPAPL FEPGELSSWS
60 70 80 90 100
FYRAGIAEFI ATFLFLYITV LTVMGVKRAP NMCASVGIQG IAWAFGGMIF
110 120 130 140 150
ALVYCTAGIS GGHINPAVTF GLFLARKLSL TRAVFYMIMQ CLGAICGAGV
160 170 180 190 200
VKGFQPTPYQ TLGGGANTVA HGYTKGSGLG AEIIGTFVLV YTVFSATDAK
210 220 230 240 250
RSARDSHVPI LAPLPIGFAV FLVHLATIPI TGTGINPARS LGAAIIYNKD
260 270 280
HSWDDHWIFW VGPFIGAALA ALYHQIVIRA IPFKSKS
Length:287
Mass (Da):30,693
Last modified:November 1, 1996 - v1
Checksum:i8CE76043F389B3B8
GO

Sequence cautioni

The sequence AAB62824 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF02782 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80801 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25A → V in BAA22097 (PubMed:8980528).Curated1
Sequence conflicti39P → S in BAA22097 (PubMed:8980528).Curated1
Sequence conflicti169V → G in BAA22097 (PubMed:8980528).Curated1
Sequence conflicti199A → D in BAA22097 (PubMed:8980528).Curated1
Sequence conflicti203A → V in BAA22097 (PubMed:8980528).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26609 mRNA. Translation: BAA05654.1.
D85192 mRNA. Translation: BAA22097.1.
AF013293 Genomic DNA. Translation: AAB62824.1. Sequence problems.
AF195115 Genomic DNA. Translation: AAF02782.1. Sequence problems.
AL161471 Genomic DNA. Translation: CAB80801.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE81879.1.
AY099825 mRNA. Translation: AAM20676.1.
AY120785 mRNA. Translation: AAM53343.1.
BT000330 mRNA. Translation: AAN15649.1.
BT006313 mRNA. Translation: AAP13421.1.
PIRiT01528.
RefSeqiNP_567178.1. NM_116268.4. [Q39196-1]
UniGeneiAt.20518.
At.20892.

Genome annotation databases

EnsemblPlantsiAT4G00430.1; AT4G00430.1; AT4G00430. [Q39196-1]
GeneIDi827956.
GrameneiAT4G00430.1; AT4G00430.1; AT4G00430. [Q39196-1]
KEGGiath:AT4G00430.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPIP14_ARATH
AccessioniPrimary (citable) accession number: Q39196
Secondary accession number(s): O23059, O23829
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families