Q39196 (PIP14_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable aquaporin PIP1-4

Alternative name(s):
Plasma membrane intrinsic protein 1-4
Short name=AtPIP1;4
Transmembrane protein C
Short name=TMP-C

Gene names

Name:	PIP1.4
Synonyms:	TMPC
Ordered Locus Names:	At4g00430
ORF Names:	A_IG005I10.2, F5I10.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aquaporins facilitate the transport of water and small neutral solutes across cell membranes By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Predominantly expressed in roots and green siliques. Also expressed above ground and in flower buds. Ref.6
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily. [View classification]
Sequence caution	The sequence AAB62824.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAF02782.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80801.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane Transmembrane helix
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to water deprivation Inferred from expression pattern PubMed 16235111. Source: TAIR
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 15060130PubMed 22923678. Source: TAIR
Molecular_function	water channel activity Inferred from sequence or structural similarity PubMed 11500536. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q39196-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Probable aquaporin PIP1-4		PRO_0000064049
Regions
	Topological domain	1 – 55	55	Cytoplasmic Potential
	Transmembrane	56 – 76	21	Helical; Name=1; Potential
	Topological domain	77 – 92	16	Extracellular Potential
	Transmembrane	93 – 113	21	Helical; Name=2; Potential
	Topological domain	114 – 133	20	Cytoplasmic Potential
	Transmembrane	134 – 154	21	Helical; Name=3; Potential
	Topological domain	155 – 175	21	Extracellular Potential
	Transmembrane	176 – 196	21	Helical; Name=4; Potential
	Topological domain	197 – 209	13	Cytoplasmic Potential
	Transmembrane	210 – 230	21	Helical; Name=5; Potential
	Topological domain	231 – 257	27	Extracellular Potential
	Transmembrane	258 – 278	21	Helical; Name=6; Potential
	Topological domain	279 – 287	9	Cytoplasmic Potential
	Motif	115 – 117	3	NPA 1
	Motif	236 – 238	3	NPA 2
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.7
Experimental info
	Sequence conflict	25	1	A → V in BAA22097. Ref.2
	Sequence conflict	39	1	P → S in BAA22097. Ref.2
	Sequence conflict	169	1	V → G in BAA22097. Ref.2
	Sequence conflict	199	1	A → D in BAA22097. Ref.2
	Sequence conflict	203	1	A → V in BAA22097. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8CE76043F389B3B8	FASTA	287	30,693
	10 20 30 40 50 60 MEGKEEDVRV GANKFPERQP IGTSAQSTDK DYKEPPPAPL FEPGELSSWS FYRAGIAEFI 70 80 90 100 110 120 ATFLFLYITV LTVMGVKRAP NMCASVGIQG IAWAFGGMIF ALVYCTAGIS GGHINPAVTF 130 140 150 160 170 180 GLFLARKLSL TRAVFYMIMQ CLGAICGAGV VKGFQPTPYQ TLGGGANTVA HGYTKGSGLG 190 200 210 220 230 240 AEIIGTFVLV YTVFSATDAK RSARDSHVPI LAPLPIGFAV FLVHLATIPI TGTGINPARS 250 260 270 280 LGAAIIYNKD HSWDDHWIFW VGPFIGAALA ALYHQIVIRA IPFKSKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a transmembrane protein (TMP-C) cDNA in Arabidopsis thaliana." Kinoshita T., Hara-Nishimura I., Shiraishi H., Okada K., Shimura Y., Nishimura M. Plant Physiol. 105:1441-1442(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Flower bud.
[2]	"Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana." Utsugi S., Sakamoto W., Ogura Y., Murata M., Motoyoshi F. Plant Mol. Biol. 32:759-765(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Flower bud.
[3]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"From genome to function: the Arabidopsis aquaporins." Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J. Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[7]	"Methylation of aquaporins in plant plasma membrane." Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C. Biochem. J. 400:189-197(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT MET-1, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26609 mRNA. Translation: BAA05654.1. D85192 mRNA. Translation: BAA22097.1. AF013293 Genomic DNA. Translation: AAB62824.1. Sequence problems. AF195115 Genomic DNA. Translation: AAF02782.1. Sequence problems. AL161471 Genomic DNA. Translation: CAB80801.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE81879.1. AY099825 mRNA. Translation: AAM20676.1. AY120785 mRNA. Translation: AAM53343.1. BT000330 mRNA. Translation: AAN15649.1. BT006313 mRNA. Translation: AAP13421.1.
IPI	IPI00521990.
PIR	T01528.
RefSeq	NP_567178.1. NM_116268.3.
UniGene	At.20518. At.20892.
3D structure databases
ProteinModelPortal	Q39196.
SMR	Q39196. Positions 44-277.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39196. 5 interactions.
Proteomic databases
PaxDb	Q39196.
PRIDE	Q39196.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G00430.1; AT4G00430.1; AT4G00430.
GeneID	827956.
KEGG	ath:AT4G00430.
Organism-specific databases
GeneFarm	2265. 182.
TAIR	At4g00430.
Phylogenomic databases
eggNOG	COG0580.
HOGENOM	HOG000288286.
InParanoid	Q39196.
KO	K09872.
OMA	GAAQDNV.
PhylomeDB	Q39196.
ProtClustDB	CLSN2682869.
Gene expression databases
ArrayExpress	Q39196.
Genevestigator	Q39196.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIP14_ARATH

Accession

Primary (citable) accession number: Q39196
Secondary accession number(s): O23059, O23829

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program