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Protein

DEAD-box ATP-dependent RNA helicase 7

Gene

RH7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 147ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • DEAD/H-box RNA helicase binding Source: TAIR
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • RNA metabolic process Source: TAIR
  • RNA secondary structure unwinding Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DEAD-box ATP-dependent RNA helicase 7 (EC:3.6.4.13)
Gene namesi
Name:RH7
Synonyms:PRH75
Ordered Locus Names:At5g62190
ORF Names:MMI9.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G62190.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002391481 – 671DEAD-box ATP-dependent RNA helicase 7Add BLAST671

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40PhosphoserineCombined sources1
Modified residuei42PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39189.
PRIDEiQ39189.

PTM databases

iPTMnetiQ39189.

Expressioni

Gene expression databases

GenevisibleiQ39189. AT.

Interactioni

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: TAIR

Protein-protein interaction databases

BioGridi21584. 4 interactors.
IntActiQ39189. 2 interactors.
STRINGi3702.AT5G62190.1.

Structurei

3D structure databases

ProteinModelPortaliQ39189.
SMRiQ39189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini127 – 309Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST183
Domaini339 – 479Helicase C-terminalPROSITE-ProRule annotationAdd BLAST141

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 124Q motifAdd BLAST29
Motifi255 – 258DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi637 – 668Gly-richAdd BLAST32

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
HOGENOMiHOG000268805.
InParanoidiQ39189.
KOiK16911.
OMAiICITLAK.
OrthoDBiEOG0936065X.
PhylomeDBiQ39189.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR012562. GUCT.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08152. GUCT. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLMLSDKK EEKKMKKKMA LDTPELDSKK GKKEQKLKLS DSDEEESEKK
60 70 80 90 100
KSKKKDKKRK ASEEEDEVKS DSSSEKKKSS KKVKLGVEDV EVDNPNAVSK
110 120 130 140 150
FRISAPLREK LKANGIEALF PIQASTFDMV LDGADLVGRA RTGQGKTLAF
160 170 180 190 200
VLPILESLVN GPAKSKRKMG YGRSPSVLVL LPTRELAKQV AADFDAYGGS
210 220 230 240 250
LGLSSCCLYG GDSYPVQEGK LKRGVDIVVG TPGRIKDHIE RQNLDFSYLQ
260 270 280 290 300
FRVLDEADEM LRMGFVEDVE LILGKVEDST KVQTLLFSAT LPSWVKNISN
310 320 330 340 350
RFLKRDQKTI DLVGNDKMKA SNSVRHIAIP CNKAAMARLI PDIISCYSSG
360 370 380 390 400
GQTIIFAETK VQVSELSGLL DGSRALHGEI PQSQREVTLA GFRNGKFATL
410 420 430 440 450
VATNVAARGL DINDVQLIIQ CEPPREVEAY IHRSGRTGRA GNTGVAVTLY
460 470 480 490 500
DSRKSSVSRI EKEAGIKFEH LAAPQPDEIA RSGGMEAAEK VKQVCDSVVP
510 520 530 540 550
AFLEAAKELL ETSGLSAEVL LAKALAKTAG FTEIKKRSLL TSMENYVTLH
560 570 580 590 600
LEAGKPIYSP SFVYGLLRRV LPDDKVEMIE GLSLTADKTG AVFDVKQSDL
610 620 630 640 650
DLFIAGAQKS AGSMSLEVVK VMPKLQEREP LPQKRFGGGG RGNRFGGGGG
660 670
NRFGGGGGRG RGGSGGRGQR Y
Length:671
Mass (Da):72,891
Last modified:May 30, 2006 - v2
Checksum:iFBFD888C79F7E35C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104S → P in AAK62631 (PubMed:10718197).Curated1
Sequence conflicti196A → E in AAK62631 (PubMed:10718197).Curated1
Sequence conflicti214Y → I in CAA68194 (PubMed:9121476).Curated1
Sequence conflicti249 – 250LQ → FK in CAA68194 (PubMed:9121476).Curated2
Sequence conflicti446A → P in CAA68194 (PubMed:9121476).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99938 mRNA. Translation: CAA68194.1.
AB019235 Genomic DNA. Translation: BAA97183.1.
CP002688 Genomic DNA. Translation: AED97579.1.
AY039576 mRNA. Translation: AAK62631.1.
AY056137 mRNA. Translation: AAL07216.1.
BT008581 mRNA. Translation: AAP40408.1.
RefSeqiNP_201025.1. NM_125613.3.
UniGeneiAt.22598.

Genome annotation databases

EnsemblPlantsiAT5G62190.1; AT5G62190.1; AT5G62190.
GeneIDi836340.
GrameneiAT5G62190.1; AT5G62190.1; AT5G62190.
KEGGiath:AT5G62190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99938 mRNA. Translation: CAA68194.1.
AB019235 Genomic DNA. Translation: BAA97183.1.
CP002688 Genomic DNA. Translation: AED97579.1.
AY039576 mRNA. Translation: AAK62631.1.
AY056137 mRNA. Translation: AAL07216.1.
BT008581 mRNA. Translation: AAP40408.1.
RefSeqiNP_201025.1. NM_125613.3.
UniGeneiAt.22598.

3D structure databases

ProteinModelPortaliQ39189.
SMRiQ39189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21584. 4 interactors.
IntActiQ39189. 2 interactors.
STRINGi3702.AT5G62190.1.

PTM databases

iPTMnetiQ39189.

Proteomic databases

PaxDbiQ39189.
PRIDEiQ39189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G62190.1; AT5G62190.1; AT5G62190.
GeneIDi836340.
GrameneiAT5G62190.1; AT5G62190.1; AT5G62190.
KEGGiath:AT5G62190.

Organism-specific databases

TAIRiAT5G62190.

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
HOGENOMiHOG000268805.
InParanoidiQ39189.
KOiK16911.
OMAiICITLAK.
OrthoDBiEOG0936065X.
PhylomeDBiQ39189.

Miscellaneous databases

PROiQ39189.

Gene expression databases

GenevisibleiQ39189. AT.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR012562. GUCT.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08152. GUCT. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRH7_ARATH
AccessioniPrimary (citable) accession number: Q39189
Secondary accession number(s): Q94BX7, Q9LVB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.