Reviewed, UniProtKB/Swiss-Prot Q39173 (P2_ARATH)

Names and origin

Protein names

Recommended name:
    Probable NADP-dependent oxidoreductase P2
    EC=1.3.1.74

Gene names

Name:	P2
Ordered Locus Names:	At5g16990
ORF Names:	F2K13_140

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a distinct role in plant antioxidant defense and is possibly involved in NAD(P)/NAD(P)h homeostasis.
Catalytic activity	n-alkanal + NAD(P)+ = alk-2-enal + NAD(P)H.
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Ref.1 Inferred from expression pattern. Source: TAIR
Cellular component	plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	2-alkenal reductase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Probable NADP-dependent oxidoreductase P2		PRO_0000218074
Experimental info
	Sequence conflict	15	1	Missing in CAA89262. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q39173-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 491698EF58BA82DA	FASTA	343	37,989
	10 20 30 40 50 60 MTTNKQVIFK DHVSGFPKES DFNFTTTTVE LRVPEGSKSV LVKNLYLSCD PYMRSRMGKP 70 80 90 100 110 120 DPSSALAQAY APGKPIYGYG VSRVIESGHP DYKKGDLLWG IVGWEEYSVI TPMAHMHFKI 130 140 150 160 170 180 QHTDVPLSYY TGLLGMPGMT AYAGFYEVCS PKKGETVYVS AASGAVGQLV GQFAKMMGCY 190 200 210 220 230 240 VVGSAGSKEK VDLLKTKFGF DDAFNYKEES DLSAALKRCF PKGIDMYFEN VGGKMLDAVL 250 260 270 280 290 300 LNMNPHGRIA VCGMISQYNL ENQEGVHNLS NIIYKRIRIQ GFVVADFYDK YPKFLELVLP 310 320 330 340 RIKEGKITYV EDVADGLEKA PEALVGLFHG KNVGKQVVVI ARE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide." Babiychuk E., Kushnir S., Belles-Boix E., van Montagu M., Inze D. J. Biol. Chem. 270:26224-26231(1995) [PubMed: 7592828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49268 mRNA. Translation: CAA89262.1. AL391141 Genomic DNA. Translation: CAC01712.1. AY091320 mRNA. Translation: AAM14259.1. AY065253 mRNA. Translation: AAL38729.1.
IPI	IPI00517835.
PIR	S57612. T51554.
RefSeq	NP_197201.1.
UniGene	At.22439
3D structure databases
SMR	Q39173. Positions 3-343.
ModBase	Search...
Proteomic databases
PRIDE	Q39173.
Genome annotation databases
GeneID	831562.
GenomeReviews	Gene locus AT5G16990 in contig BA000015_GR.
KEGG	ath:AT5G16990.
NMPDR	fig|3702.1.peg.23861.
Organism-specific databases
TAIR	At5g16990.
Phylogenomic databases
eggNOG	KOG1196.
HOGENOM	HBG753318.
InParanoid	Q39173.
OMA	HEHRTIG.
PhylomeDB	Q39173.
Enzyme and pathway databases
BRENDA	1.3.1.74. 302.
Gene expression databases
ArrayExpress	Q39173.
Genevestigator	Q39173.
GermOnline	AT5G16990. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2_ARATH

Accession

Primary (citable) accession number: Q39173
Secondary accession number(s): Q9LFK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 6, 2002
Last modified:	February 9, 2010
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)