Q39172 (P1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-dependent alkenal double bond reductase P1
Short name=DBR1
EC=1.3.1.74

Gene names

Name:	P1
Ordered Locus Names:	At5g16970
ORF Names:	F2K13_120

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro). Has activity towards toxic substrates, such as 4-hydroxy-(2E)-nonenal (in vitro). May play a distinct role in plant antioxidant defense and is possibly involved in NAD(P)/NAD(P)H homeostasis. Ref.4
Catalytic activity	n-alkanal + NAD(P)+ = alk-2-enal + NAD(P)H. Ref.4
Subunit structure	Homodimer. Ref.4
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.
Biophysicochemical properties	Kinetic parameters: KM=0.53 mM for p-coumaryl aldehyde Ref.4 KM=0.41 mM for coniferyl aldehyde KM=0.28 mM for 4-hydroxy-(2E)-nonenal

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to cadmium ion Inferred from expression pattern. Source: TAIR response to oxidative stress Inferred from mutant phenotype. Source: TAIR
Cellular component	cytosol Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	2-alkenal reductase [NAD(P)] activity Inferred from direct assay. Source: TAIR nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 345

345

NADP-dependent alkenal double bond reductase P1

PRO_0000218073

Regions

Nucleotide binding

166 – 169

4

NADP

Nucleotide binding

254 – 260

7

NADP

Nucleotide binding

284 – 286

3

NADP

Sites

Binding site

53

1

Substrate

Binding site

81

1

Substrate

Binding site

192

1

NADP

Binding site

208

1

NADP

Binding site

232

1

NADP

Binding site

334

1

NADP

Secondary structure

1

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345

Helix Strand Turn

Details...

Beta strand

2 – 10

9

Beta strand

14 – 17

4

Helix

20 – 22

3

Beta strand

23 – 31

9

Beta strand

36 – 39

4

Beta strand

41 – 45

5

Beta strand

47 – 49

3

Helix

54 – 58

5

Beta strand

79 – 89

11

Beta strand

99 – 112

14

Turn

116 – 118

3

Beta strand

120 – 122

3

Helix

131 – 133

3

Turn

134 – 136

3

Helix

138 – 148

11

Beta strand

158 – 163

6

Helix

167 – 178

12

Beta strand

182 – 189

8

Helix

190 – 198

9

Beta strand

203 – 207

5

Helix

215 – 221

7

Beta strand

226 – 233

8

Helix

235 – 242

8

Beta strand

245 – 253

9

Helix

257 – 259

3

Helix

273 – 277

5

Beta strand

280 – 283

4

Helix

286 – 292

7

Helix

293 – 305

13

Beta strand

313 – 318

6

Helix

319 – 321

3

Helix

323 – 330

8

Beta strand

335 – 343

9

Sequences

	Sequence		Length	Mass (Da)
	Q39172 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5AFCEBB2948B2680	FASTA	345	38,134
	10 20 30 40 50 60 MTATNKQVIL KDYVSGFPTE SDFDFTTTTV ELRVPEGTNS VLVKNLYLSC DPYMRIRMGK 70 80 90 100 110 120 PDPSTAALAQ AYTPGQPIQG YGVSRIIESG HPDYKKGDLL WGIVAWEEYS VITPMTHAHF 130 140 150 160 170 180 KIQHTDVPLS YYTGLLGMPG MTAYAGFYEV CSPKEGETVY VSAASGAVGQ LVGQLAKMMG 190 200 210 220 230 240 CYVVGSAGSK EKVDLLKTKF GFDDAFNYKE ESDLTAALKR CFPNGIDIYF ENVGGKMLDA 250 260 270 280 290 300 VLVNMNMHGR IAVCGMISQY NLENQEGVHN LSNIIYKRIR IQGFVVSDFY DKYSKFLEFV 310 320 330 340 LPHIREGKIT YVEDVADGLE KAPEALVGLF HGKNVGKQVV VVARE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide." Babiychuk E., Kushnir S., Belles-Boix E., van Montagu M., Inze D. J. Biol. Chem. 270:26224-26231(1995) [PubMed: 7592828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Mechanistic and structural studies of apoform, binary, and ternary complexes of the Arabidopsis alkenal double bond reductase At5g16970." Youn B., Kim S.J., Moinuddin S.G., Lee C., Bedgar D.L., Harper A.R., Davin L.B., Lewis N.G., Kang C. J. Biol. Chem. 281:40076-40088(2006) [PubMed: 17028190] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEXES WITH SUBSTRATES AND NADP, CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49768 mRNA. Translation: CAA89838.1.
AL391141 Genomic DNA. Translation: CAC01710.1.
CP002688 Genomic DNA. Translation: AED92364.1.

IPI

IPI00542017.

PIR

S57611.

RefSeq

NP_197199.1. NM_121703.3.

UniGene

At.22432.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J3H	X-ray	2.50	A/B	1-345	[»]
2J3I	X-ray	2.80	A/B	1-345	[»]
2J3J	X-ray	2.80	A/B	1-345	[»]
2J3K	X-ray	2.80	A/B	1-345	[»]

ProteinModelPortal

Q39172.

SMR

Q39172. Positions 1-345.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q39172. 1 interaction.

STRING

Q39172.

Proteomic databases

PRIDE

Q39172.

ProMEX

Q39172.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT5G16970.1; AT5G16970.1; AT5G16970.

GeneID

831560.

GenomeReviews

Gene locus AT5G16970 in contig BA000015_GR.

KEGG

ath:AT5G16970.

Organism-specific databases

TAIR

At5g16970.

Phylogenomic databases

eggNOG

KOG1196.

HOGENOM

HBG753318.

InParanoid

Q39172.

OMA

VINYRET.

PhylomeDB

Q39172.

ProtClustDB

CLSN2682646.

Enzyme and pathway databases

BioCyc

MetaCyc:AT5G16970-MONOMER.

Gene expression databases

Genevestigator

Q39172.

GermOnline

AT5G16970. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

KO

K08070.

PANTHER

PTHR11695. ADH_Sf_Zn. 1 hit.

Pfam

PF00107. ADH_zinc_N. 1 hit.
[Graphical view]

SUPFAM

SSF50129. GroES_like. 2 hits.

ProtoNet

Search...

Entry information

Entry name

P1_ARATH

Accession

Primary (citable) accession number: Q39172

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program