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Q39162 (TGA4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor TGA4
Alternative name(s):
Ocs element-binding factor 4
Short name=OBF4
bZIP transcription factor 57
Short name=AtbZIP57
Gene names
Name:TGA4
Synonyms:BZIP57, OBF4
Ordered Locus Names:At5g10030
ORF Names:T31P16_20
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters.

Subunit structure

Binds DNA as a dimer. Interaction with the Dof domain proteins OBP1, OBP2 or OBP3 enhances the binding to the ocs element. Interacts with RAP2-3/EPB, an ethylene-responsive element binding protein. The reduced form interacts with NPR1. Ref.4 Ref.6 Ref.7 Ref.9

Subcellular location

Nucleus.

Tissue specificity

Predominantly expressed in roots. Ref.5

Sequence similarities

Belongs to the bZIP family.

Contains 1 bZIP (basic-leucine zipper) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

COQ390573EBI-541600,EBI-1639724

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 364364Transcription factor TGA4
PRO_0000076556

Regions

Domain78 – 14164bZIP
Region80 – 10021Basic motif By similarity
Region106 – 12015Leucine-zipper By similarity
Coiled coil79 – 12749 Potential
Coiled coil257 – 27721 Potential

Amino acid modifications

Disulfide bond256 ↔ 262 Potential

Experimental info

Mutagenesis2561C → N: Gain of interaction with NPR1; when associated with S-262. Ref.9
Mutagenesis2621C → S: Gain of interaction with NPR1; when associated with N-256. Ref.9
Sequence conflict2651A → S in CAA49524. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q39162 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: 08225A91F4CA20ED

FASTA36441,751
        10         20         30         40         50         60 
MNTTSTHFVP PRRFEVYEPL NQIGMWEESF KNNGDMYTPG SIIIPTNEKP DSLSEDTSHG 

        70         80         90        100        110        120 
TEGTPHKFDQ EASTSRHPDK IQRRLAQNRE AARKSRLRKK AYVQQLETSR LKLIHLEQEL 

       130        140        150        160        170        180 
DRARQQGFYV GNGVDTNALS FSDNMSSGIV AFEMEYGHWV EEQNRQICEL RTVLHGQVSD 

       190        200        210        220        230        240 
IELRSLVENA MKHYFQLFRM KSAAAKIDVF YVMSGMWKTS AERFFLWIGG FRPSELLKVL 

       250        260        270        280        290        300 
LPHFDPLTDQ QLLDVCNLRQ SCQQAEDALS QGMEKLQHTL AESVAAGKLG EGSYIPQMTC 

       310        320        330        340        350        360 
AMERLEALVS FVNQADHLRH ETLQQMHRIL TTRQAARGLL ALGEYFQRLR ALSSSWAARQ 


REPT 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of two related Arabidopsis ocs-element bZIP binding proteins."
Zhang B., Foley R.C., Singh K.B.
Plant J. 4:711-716(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: cv. Columbia.
Tissue: Root.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences."
Zhang B., Chen W., Foley R.C., Buettner M., Singh K.B.
Plant Cell 7:2241-2252(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OBP1.
[5]"Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2."
de Pater S., Pham K., Memelink J., Kijne J.
Mol. Gen. Genet. 250:237-239(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein."
Buettner M., Singh K.B.
Proc. Natl. Acad. Sci. U.S.A. 94:5961-5966(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EBP.
[7]"Characterization of salicylic acid-responsive, Arabidopsis Dof domain proteins: overexpression of OBP3 leads to growth defects."
Kang H.-G., Singh K.B.
Plant J. 21:329-339(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OBP2 AND OBP3.
[8]"bZIP transcription factors in Arabidopsis."
Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J., Tiedemann J., Kroj T., Parcy F.
Trends Plant Sci. 7:106-111(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1."
Despres C., Chubak C., Rochon A., Clark R., Bethune T., Desveaux D., Fobert P.R.
Plant Cell 15:2181-2191(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NPR1, MUTAGENESIS OF CYS-256 AND CYS-262.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69899 mRNA. Translation: CAA49524.1.
AL356332 Genomic DNA. Translation: CAB92044.1.
CP002688 Genomic DNA. Translation: AED91484.1.
CP002688 Genomic DNA. Translation: AED91485.1.
PIRS48121.
T50007.
RefSeqNP_001190270.1. NM_001203341.1.
NP_196565.1. NM_121041.4.
UniGeneAt.24071.

3D structure databases

ProteinModelPortalQ39162.
SMRQ39162. Positions 84-126.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid16144. 2 interactions.
IntActQ39162. 7 interactions.

Proteomic databases

PaxDbQ39162.
PRIDEQ39162.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G10030.1; AT5G10030.1; AT5G10030.
AT5G10030.2; AT5G10030.2; AT5G10030.
GeneID830866.
KEGGath:AT5G10030.

Organism-specific databases

GeneFarm2995. 292.
TAIRAT5G10030.

Phylogenomic databases

eggNOGNOG298593.
HOGENOMHOG000116597.
InParanoidQ39162.
KOK14431.
OMACAMERLE.
PhylomeDBQ39162.

Gene expression databases

GenevestigatorQ39162.

Family and domain databases

InterProIPR004827. bZIP.
IPR025422. TGA_domain.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
PF14144. DOG1. 1 hit.
[Graphical view]
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTGA4_ARATH
AccessionPrimary (citable) accession number: Q39162
Secondary accession number(s): Q9LX25
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names