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Protein

Transcription factor TGA4

Gene

TGA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters.

GO - Molecular functioni

  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • response to cold Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor TGA4
Alternative name(s):
Ocs element-binding factor 4
Short name:
OBF4
bZIP transcription factor 57
Short name:
AtbZIP57
Gene namesi
Name:TGA4
Synonyms:BZIP57, OBF4
Ordered Locus Names:At5g10030
ORF Names:T31P16_20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G10030.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi256C → N: Gain of interaction with NPR1; when associated with S-262. 1 Publication1
Mutagenesisi262C → S: Gain of interaction with NPR1; when associated with N-256. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765561 – 364Transcription factor TGA4Add BLAST364

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi256 ↔ 262Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ39162.
PRIDEiQ39162.

Expressioni

Tissue specificityi

Predominantly expressed in roots.1 Publication

Gene expression databases

ExpressionAtlasiQ39162. baseline and differential.
GenevisibleiQ39162. AT.

Interactioni

Subunit structurei

Binds DNA as a dimer. Interaction with the Dof domain proteins OBP1, OBP2 or OBP3 enhances the binding to the ocs element. Interacts with RAP2-3/EPB, an ethylene-responsive element binding protein. The reduced form interacts with NPR1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COQ390573EBI-541600,EBI-1639724

Protein-protein interaction databases

BioGridi16144. 6 interactors.
IntActiQ39162. 7 interactors.
STRINGi3702.AT5G10030.1.

Structurei

3D structure databases

ProteinModelPortaliQ39162.
SMRiQ39162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 141bZIPPROSITE-ProRule annotationAdd BLAST64
Domaini149 – 359DOG1PROSITE-ProRule annotationAdd BLAST211

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 100Basic motifPROSITE-ProRule annotationAdd BLAST21
Regioni106 – 120Leucine-zipperPROSITE-ProRule annotationAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili79 – 127Sequence analysisAdd BLAST49
Coiled coili257 – 277Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the bZIP family.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation
Contains 1 DOG1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IKSN. Eukaryota.
ENOG410YEGW. LUCA.
HOGENOMiHOG000116597.
InParanoidiQ39162.
KOiK14431.
OMAiCAMERLE.
OrthoDBiEOG09360E5Y.
PhylomeDBiQ39162.

Family and domain databases

InterProiIPR004827. bZIP.
IPR025422. TGA_domain.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
PF14144. DOG1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
PS51806. DOG1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39162-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTTSTHFVP PRRFEVYEPL NQIGMWEESF KNNGDMYTPG SIIIPTNEKP
60 70 80 90 100
DSLSEDTSHG TEGTPHKFDQ EASTSRHPDK IQRRLAQNRE AARKSRLRKK
110 120 130 140 150
AYVQQLETSR LKLIHLEQEL DRARQQGFYV GNGVDTNALS FSDNMSSGIV
160 170 180 190 200
AFEMEYGHWV EEQNRQICEL RTVLHGQVSD IELRSLVENA MKHYFQLFRM
210 220 230 240 250
KSAAAKIDVF YVMSGMWKTS AERFFLWIGG FRPSELLKVL LPHFDPLTDQ
260 270 280 290 300
QLLDVCNLRQ SCQQAEDALS QGMEKLQHTL AESVAAGKLG EGSYIPQMTC
310 320 330 340 350
AMERLEALVS FVNQADHLRH ETLQQMHRIL TTRQAARGLL ALGEYFQRLR
360
ALSSSWAARQ REPT
Length:364
Mass (Da):41,751
Last modified:March 1, 2004 - v2
Checksum:i08225A91F4CA20ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265A → S in CAA49524 (PubMed:8252072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69899 mRNA. Translation: CAA49524.1.
AL356332 Genomic DNA. Translation: CAB92044.1.
CP002688 Genomic DNA. Translation: AED91484.1.
CP002688 Genomic DNA. Translation: AED91485.1.
PIRiS48121.
T50007.
RefSeqiNP_001190270.1. NM_001203341.1.
NP_196565.1. NM_121041.5.
UniGeneiAt.24071.

Genome annotation databases

EnsemblPlantsiAT5G10030.1; AT5G10030.1; AT5G10030.
AT5G10030.2; AT5G10030.2; AT5G10030.
GeneIDi830866.
GrameneiAT5G10030.1; AT5G10030.1; AT5G10030.
AT5G10030.2; AT5G10030.2; AT5G10030.
KEGGiath:AT5G10030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69899 mRNA. Translation: CAA49524.1.
AL356332 Genomic DNA. Translation: CAB92044.1.
CP002688 Genomic DNA. Translation: AED91484.1.
CP002688 Genomic DNA. Translation: AED91485.1.
PIRiS48121.
T50007.
RefSeqiNP_001190270.1. NM_001203341.1.
NP_196565.1. NM_121041.5.
UniGeneiAt.24071.

3D structure databases

ProteinModelPortaliQ39162.
SMRiQ39162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16144. 6 interactors.
IntActiQ39162. 7 interactors.
STRINGi3702.AT5G10030.1.

Proteomic databases

PaxDbiQ39162.
PRIDEiQ39162.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G10030.1; AT5G10030.1; AT5G10030.
AT5G10030.2; AT5G10030.2; AT5G10030.
GeneIDi830866.
GrameneiAT5G10030.1; AT5G10030.1; AT5G10030.
AT5G10030.2; AT5G10030.2; AT5G10030.
KEGGiath:AT5G10030.

Organism-specific databases

TAIRiAT5G10030.

Phylogenomic databases

eggNOGiENOG410IKSN. Eukaryota.
ENOG410YEGW. LUCA.
HOGENOMiHOG000116597.
InParanoidiQ39162.
KOiK14431.
OMAiCAMERLE.
OrthoDBiEOG09360E5Y.
PhylomeDBiQ39162.

Miscellaneous databases

PROiQ39162.

Gene expression databases

ExpressionAtlasiQ39162. baseline and differential.
GenevisibleiQ39162. AT.

Family and domain databases

InterProiIPR004827. bZIP.
IPR025422. TGA_domain.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
PF14144. DOG1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
PS51806. DOG1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGA4_ARATH
AccessioniPrimary (citable) accession number: Q39162
Secondary accession number(s): Q9LX25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.