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Q39162

- TGA4_ARATH

UniProt

Q39162 - TGA4_ARATH

Protein

Transcription factor TGA4

Gene

TGA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
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    Functioni

    Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. sequence-specific DNA binding Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. defense response to bacterium Source: TAIR
    2. response to cold Source: TAIR
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor TGA4
    Alternative name(s):
    Ocs element-binding factor 4
    Short name:
    OBF4
    bZIP transcription factor 57
    Short name:
    AtbZIP57
    Gene namesi
    Name:TGA4
    Synonyms:BZIP57, OBF4
    Ordered Locus Names:At5g10030
    ORF Names:T31P16_20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G10030.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi256 – 2561C → N: Gain of interaction with NPR1; when associated with S-262. 1 Publication
    Mutagenesisi262 – 2621C → S: Gain of interaction with NPR1; when associated with N-256. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364Transcription factor TGA4PRO_0000076556Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi256 ↔ 262Sequence Analysis

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ39162.
    PRIDEiQ39162.

    Expressioni

    Tissue specificityi

    Predominantly expressed in roots.1 Publication

    Gene expression databases

    GenevestigatoriQ39162.

    Interactioni

    Subunit structurei

    Binds DNA as a dimer. Interaction with the Dof domain proteins OBP1, OBP2 or OBP3 enhances the binding to the ocs element. Interacts with RAP2-3/EPB, an ethylene-responsive element binding protein. The reduced form interacts with NPR1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    COQ390573EBI-541600,EBI-1639724

    Protein-protein interaction databases

    BioGridi16144. 2 interactions.
    IntActiQ39162. 7 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39162.
    SMRiQ39162. Positions 84-126.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini78 – 14164bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 10021Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni106 – 12015Leucine-zipperPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili79 – 12749Sequence AnalysisAdd
    BLAST
    Coiled coili257 – 27721Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bZIP family.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG298593.
    HOGENOMiHOG000116597.
    InParanoidiQ39162.
    KOiK14431.
    OMAiCAMERLE.
    PhylomeDBiQ39162.

    Family and domain databases

    InterProiIPR004827. bZIP.
    IPR025422. TGA_domain.
    [Graphical view]
    PfamiPF00170. bZIP_1. 1 hit.
    PF14144. DOG1. 1 hit.
    [Graphical view]
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    PROSITEiPS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q39162-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTTSTHFVP PRRFEVYEPL NQIGMWEESF KNNGDMYTPG SIIIPTNEKP    50
    DSLSEDTSHG TEGTPHKFDQ EASTSRHPDK IQRRLAQNRE AARKSRLRKK 100
    AYVQQLETSR LKLIHLEQEL DRARQQGFYV GNGVDTNALS FSDNMSSGIV 150
    AFEMEYGHWV EEQNRQICEL RTVLHGQVSD IELRSLVENA MKHYFQLFRM 200
    KSAAAKIDVF YVMSGMWKTS AERFFLWIGG FRPSELLKVL LPHFDPLTDQ 250
    QLLDVCNLRQ SCQQAEDALS QGMEKLQHTL AESVAAGKLG EGSYIPQMTC 300
    AMERLEALVS FVNQADHLRH ETLQQMHRIL TTRQAARGLL ALGEYFQRLR 350
    ALSSSWAARQ REPT 364
    Length:364
    Mass (Da):41,751
    Last modified:March 1, 2004 - v2
    Checksum:i08225A91F4CA20ED
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti265 – 2651A → S in CAA49524. (PubMed:8252072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69899 mRNA. Translation: CAA49524.1.
    AL356332 Genomic DNA. Translation: CAB92044.1.
    CP002688 Genomic DNA. Translation: AED91484.1.
    CP002688 Genomic DNA. Translation: AED91485.1.
    PIRiS48121.
    T50007.
    RefSeqiNP_001190270.1. NM_001203341.1.
    NP_196565.1. NM_121041.4.
    UniGeneiAt.24071.

    Genome annotation databases

    EnsemblPlantsiAT5G10030.1; AT5G10030.1; AT5G10030.
    AT5G10030.2; AT5G10030.2; AT5G10030.
    GeneIDi830866.
    KEGGiath:AT5G10030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69899 mRNA. Translation: CAA49524.1 .
    AL356332 Genomic DNA. Translation: CAB92044.1 .
    CP002688 Genomic DNA. Translation: AED91484.1 .
    CP002688 Genomic DNA. Translation: AED91485.1 .
    PIRi S48121.
    T50007.
    RefSeqi NP_001190270.1. NM_001203341.1.
    NP_196565.1. NM_121041.4.
    UniGenei At.24071.

    3D structure databases

    ProteinModelPortali Q39162.
    SMRi Q39162. Positions 84-126.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 16144. 2 interactions.
    IntActi Q39162. 7 interactions.

    Proteomic databases

    PaxDbi Q39162.
    PRIDEi Q39162.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G10030.1 ; AT5G10030.1 ; AT5G10030 .
    AT5G10030.2 ; AT5G10030.2 ; AT5G10030 .
    GeneIDi 830866.
    KEGGi ath:AT5G10030.

    Organism-specific databases

    GeneFarmi 2995. 292.
    TAIRi AT5G10030.

    Phylogenomic databases

    eggNOGi NOG298593.
    HOGENOMi HOG000116597.
    InParanoidi Q39162.
    KOi K14431.
    OMAi CAMERLE.
    PhylomeDBi Q39162.

    Gene expression databases

    Genevestigatori Q39162.

    Family and domain databases

    InterProi IPR004827. bZIP.
    IPR025422. TGA_domain.
    [Graphical view ]
    Pfami PF00170. bZIP_1. 1 hit.
    PF14144. DOG1. 1 hit.
    [Graphical view ]
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    PROSITEi PS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of two related Arabidopsis ocs-element bZIP binding proteins."
      Zhang B., Foley R.C., Singh K.B.
      Plant J. 4:711-716(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: cv. Columbia.
      Tissue: Root.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences."
      Zhang B., Chen W., Foley R.C., Buettner M., Singh K.B.
      Plant Cell 7:2241-2252(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OBP1.
    5. "Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2."
      de Pater S., Pham K., Memelink J., Kijne J.
      Mol. Gen. Genet. 250:237-239(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein."
      Buettner M., Singh K.B.
      Proc. Natl. Acad. Sci. U.S.A. 94:5961-5966(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EBP.
    7. "Characterization of salicylic acid-responsive, Arabidopsis Dof domain proteins: overexpression of OBP3 leads to growth defects."
      Kang H.-G., Singh K.B.
      Plant J. 21:329-339(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OBP2 AND OBP3.
    8. Cited for: GENE FAMILY, NOMENCLATURE.
    9. "The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1."
      Despres C., Chubak C., Rochon A., Clark R., Bethune T., Desveaux D., Fobert P.R.
      Plant Cell 15:2181-2191(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NPR1, MUTAGENESIS OF CYS-256 AND CYS-262.

    Entry informationi

    Entry nameiTGA4_ARATH
    AccessioniPrimary (citable) accession number: Q39162
    Secondary accession number(s): Q9LX25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3