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Q39162

- TGA4_ARATH

UniProt

Q39162 - TGA4_ARATH

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Protein

Transcription factor TGA4

Gene

TGA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters.

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. defense response to bacterium Source: TAIR
  2. response to cold Source: TAIR
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor TGA4
Alternative name(s):
Ocs element-binding factor 4
Short name:
OBF4
bZIP transcription factor 57
Short name:
AtbZIP57
Gene namesi
Name:TGA4
Synonyms:BZIP57, OBF4
Ordered Locus Names:At5g10030
ORF Names:T31P16_20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G10030.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi256 – 2561C → N: Gain of interaction with NPR1; when associated with S-262. 1 Publication
Mutagenesisi262 – 2621C → S: Gain of interaction with NPR1; when associated with N-256. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Transcription factor TGA4PRO_0000076556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi256 ↔ 262Sequence Analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ39162.
PRIDEiQ39162.

Expressioni

Tissue specificityi

Predominantly expressed in roots.1 Publication

Gene expression databases

GenevestigatoriQ39162.

Interactioni

Subunit structurei

Binds DNA as a dimer. Interaction with the Dof domain proteins OBP1, OBP2 or OBP3 enhances the binding to the ocs element. Interacts with RAP2-3/EPB, an ethylene-responsive element binding protein. The reduced form interacts with NPR1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COQ390573EBI-541600,EBI-1639724

Protein-protein interaction databases

BioGridi16144. 2 interactions.
IntActiQ39162. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ39162.
SMRiQ39162. Positions 84-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 14164bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 10021Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni106 – 12015Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili79 – 12749Sequence AnalysisAdd
BLAST
Coiled coili257 – 27721Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the bZIP family.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG298593.
HOGENOMiHOG000116597.
InParanoidiQ39162.
KOiK14431.
OMAiCAMERLE.
PhylomeDBiQ39162.

Family and domain databases

InterProiIPR004827. bZIP.
IPR025422. TGA_domain.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
PF14144. DOG1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39162-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTTSTHFVP PRRFEVYEPL NQIGMWEESF KNNGDMYTPG SIIIPTNEKP
60 70 80 90 100
DSLSEDTSHG TEGTPHKFDQ EASTSRHPDK IQRRLAQNRE AARKSRLRKK
110 120 130 140 150
AYVQQLETSR LKLIHLEQEL DRARQQGFYV GNGVDTNALS FSDNMSSGIV
160 170 180 190 200
AFEMEYGHWV EEQNRQICEL RTVLHGQVSD IELRSLVENA MKHYFQLFRM
210 220 230 240 250
KSAAAKIDVF YVMSGMWKTS AERFFLWIGG FRPSELLKVL LPHFDPLTDQ
260 270 280 290 300
QLLDVCNLRQ SCQQAEDALS QGMEKLQHTL AESVAAGKLG EGSYIPQMTC
310 320 330 340 350
AMERLEALVS FVNQADHLRH ETLQQMHRIL TTRQAARGLL ALGEYFQRLR
360
ALSSSWAARQ REPT
Length:364
Mass (Da):41,751
Last modified:March 1, 2004 - v2
Checksum:i08225A91F4CA20ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651A → S in CAA49524. (PubMed:8252072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69899 mRNA. Translation: CAA49524.1.
AL356332 Genomic DNA. Translation: CAB92044.1.
CP002688 Genomic DNA. Translation: AED91484.1.
CP002688 Genomic DNA. Translation: AED91485.1.
PIRiS48121.
T50007.
RefSeqiNP_001190270.1. NM_001203341.1.
NP_196565.1. NM_121041.4.
UniGeneiAt.24071.

Genome annotation databases

EnsemblPlantsiAT5G10030.1; AT5G10030.1; AT5G10030.
AT5G10030.2; AT5G10030.2; AT5G10030.
GeneIDi830866.
KEGGiath:AT5G10030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69899 mRNA. Translation: CAA49524.1 .
AL356332 Genomic DNA. Translation: CAB92044.1 .
CP002688 Genomic DNA. Translation: AED91484.1 .
CP002688 Genomic DNA. Translation: AED91485.1 .
PIRi S48121.
T50007.
RefSeqi NP_001190270.1. NM_001203341.1.
NP_196565.1. NM_121041.4.
UniGenei At.24071.

3D structure databases

ProteinModelPortali Q39162.
SMRi Q39162. Positions 84-126.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 16144. 2 interactions.
IntActi Q39162. 7 interactions.

Proteomic databases

PaxDbi Q39162.
PRIDEi Q39162.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G10030.1 ; AT5G10030.1 ; AT5G10030 .
AT5G10030.2 ; AT5G10030.2 ; AT5G10030 .
GeneIDi 830866.
KEGGi ath:AT5G10030.

Organism-specific databases

GeneFarmi 2995. 292.
TAIRi AT5G10030.

Phylogenomic databases

eggNOGi NOG298593.
HOGENOMi HOG000116597.
InParanoidi Q39162.
KOi K14431.
OMAi CAMERLE.
PhylomeDBi Q39162.

Gene expression databases

Genevestigatori Q39162.

Family and domain databases

InterProi IPR004827. bZIP.
IPR025422. TGA_domain.
[Graphical view ]
Pfami PF00170. bZIP_1. 1 hit.
PF14144. DOG1. 1 hit.
[Graphical view ]
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
PROSITEi PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of two related Arabidopsis ocs-element bZIP binding proteins."
    Zhang B., Foley R.C., Singh K.B.
    Plant J. 4:711-716(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: cv. Columbia.
    Tissue: Root.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences."
    Zhang B., Chen W., Foley R.C., Buettner M., Singh K.B.
    Plant Cell 7:2241-2252(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OBP1.
  5. "Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2."
    de Pater S., Pham K., Memelink J., Kijne J.
    Mol. Gen. Genet. 250:237-239(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein."
    Buettner M., Singh K.B.
    Proc. Natl. Acad. Sci. U.S.A. 94:5961-5966(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EBP.
  7. "Characterization of salicylic acid-responsive, Arabidopsis Dof domain proteins: overexpression of OBP3 leads to growth defects."
    Kang H.-G., Singh K.B.
    Plant J. 21:329-339(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OBP2 AND OBP3.
  8. Cited for: GENE FAMILY, NOMENCLATURE.
  9. "The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1."
    Despres C., Chubak C., Rochon A., Clark R., Bethune T., Desveaux D., Fobert P.R.
    Plant Cell 15:2181-2191(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NPR1, MUTAGENESIS OF CYS-256 AND CYS-262.

Entry informationi

Entry nameiTGA4_ARATH
AccessioniPrimary (citable) accession number: Q39162
Secondary accession number(s): Q9LX25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 26, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3