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Reviewed, UniProtKB/Swiss-Prot Q39147 (3MG_ARATH)

Last modified November 3, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA-3-methyladenine glycosylase
    EC=3.2.2.21
Alternative name(s):
    3-methyladenine DNA glycosidase
Gene names
Name: MAG
Ordered Locus Names: At3g12040
ORF Names: T21B14.14, MEC18.17, T21B14_115
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions By similarity.

Catalytic activity

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Subcellular location

Nucleus Potential.

Sequence similarities

Belongs to the DNA glycosylase MPG family.

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Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbase-excision repair

Inferred from electronic annotation. Source: InterPro

   Cellular componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

alkylbase DNA N-glycosylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254DNA-3-methyladenine glycosylase
PRO_0000100068

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Sequences

Sequence LengthMass (Da)Tools
Q39147-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0BC880710D1BE1C5

FASTA25428,133
        10         20         30         40         50         60 
MKTPARRSKR VNQEESETNV TTRVVLRTRK TNCSKTRAAR VRPDYPLTRT TSESEMKLMP 

        70         80         90        100        110        120 
PEFFQIDALD LAPRLLGKFM RRDNVVLRIT EVEAYRPNDS ACHGRFGVTP RTAPVFGPGG 

       130        140        150        160        170        180 
HAYVYLCYGL HMMLNIVADK EGVGAAVLIR SCSPVSGMET IQERRGLKTD KPVLLNGPGK 

       190        200        210        220        230        240 
VGQALGLSTE WSHHPLYSPG GLELLDGGED VEKVMVGPRV GIDYALPEHV NALWRFAVAD 

       250 
TPWISAPKNT LKPL

« Hide

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References

« Hide 'large scale' references
[1]"Cloning of a 3-methyladenine-DNA glycosylase from Arabidopsis thaliana."
Santerre A., Britt A.B.
Proc. Natl. Acad. Sci. U.S.A. 91:2240-2244(1994) [PubMed: 8134381] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Aerial part.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

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Cross-references

Sequence databases

X76169 mRNA. Translation: CAA53763.1.
AP002040 Genomic DNA. Translation: BAB03116.1.
AC069473 Genomic DNA. Translation: AAG51039.1.
IPIIPI00517837.
RefSeqNP_187811.1.
UniGeneAt.5389

3D structure databases

HSSPHSSP built from PDB template 1F4R based on UniProtKB P29372.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ39147.

Proteomic databases

PRIDEQ39147.

Genome annotation databases

GeneID820378.
GenomeReviewsGene locus AT3G12040 in contig BA000014_GR.
KEGGath:AT3G12040.

Organism-specific databases

TAIRAt3g12040.

Phylogenomic databases

OMADSTSHAF.

Enzyme and pathway databases

BRENDA3.2.2.21. 302.

Gene expression databases

ArrayExpressQ39147.
GenevestigatorQ39147.
GermOnlineAT3G12040. Arabidopsis thaliana.

Family and domain databases

InterProIPR003180. PurDNA_glycsylse.
[Graphical view]
Gene3DG3DSA:3.10.300.10. PurDNA_glycsylse. 1 hit.
PANTHERPTHR10429. PurDNA_glycsylse. 1 hit.
PfamPF02245. Pur_DNA_glyco. 1 hit.
[Graphical view]
ProDomPD009649. PurDNA_glycsylse. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00567. 3mg. 1 hit.
ProtoNetSearch...

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Entry information

Entry name3MG_ARATH
AccessionPrimary (citable) accession number: Q39147
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents