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Protein

Thiosulfate sulfurtransferase 16, chloroplastic

Gene

STR16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thought to act during the early stages of leaf senescence. Catalyzes the transfer of a sulfur ion from a donor to cyanide or to other thiol compounds. Substrate preference is thiosulfate > 3-mercaptopyruvate.1 Publication

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.1 Publication

Kineticsi

  1. KM=7.4 mM for thiosulfate1 Publication
  2. KM=51.7 mM for 3-mercaptopyruvate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei80Cysteine persulfide intermediatePROSITE-ProRule annotation1
    Binding sitei85SubstrateBy similarity1

    GO - Molecular functioni

    • thiosulfate sulfurtransferase activity Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciARA:AT5G66040-MONOMER.
    SABIO-RKQ39129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiosulfate sulfurtransferase 16, chloroplastic (EC:2.8.1.1)
    Alternative name(s):
    Rhodanese
    Senescence-associated protein
    Sulfurtransferase 16
    Short name:
    AtStr16
    Gene namesi
    Name:STR16
    Ordered Locus Names:At5g66040
    ORF Names:K2A18.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G66040.

    Subcellular locationi

    GO - Cellular componenti

    • chloroplast Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001394051 – 120Thiosulfate sulfurtransferase 16, chloroplasticAdd BLAST120

    Proteomic databases

    PaxDbiQ39129.

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ39129. baseline and differential.
    GenevisibleiQ39129. AT.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi21976. 1 interactor.
    MINTiMINT-8071292.
    STRINGi3702.AT5G66040.1.

    Structurei

    Secondary structure

    1120
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi9 – 12Combined sources4
    Helixi13 – 22Combined sources10
    Beta strandi26 – 30Combined sources5
    Helixi32 – 37Combined sources6
    Beta strandi43 – 45Combined sources3
    Turni53 – 55Combined sources3
    Turni59 – 61Combined sources3
    Helixi62 – 66Combined sources5
    Turni67 – 69Combined sources3
    Beta strandi74 – 82Combined sources9
    Helixi85 – 97Combined sources13
    Beta strandi100 – 105Combined sources6
    Helixi108 – 114Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1TQ1NMR-A2-120[»]
    ProteinModelPortaliQ39129.
    SMRiQ39129.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ39129.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini20 – 120RhodanesePROSITE-ProRule annotationAdd BLAST101

    Sequence similaritiesi

    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiKOG1530. Eukaryota.
    COG0607. LUCA.
    HOGENOMiHOG000247776.
    InParanoidiQ39129.
    OMAiSAWRENE.
    OrthoDBiEOG09360OE7.
    PhylomeDBiQ39129.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q39129-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MAEESRVPSS VSVTVAHDLL LAGHRYLDVR TPEEFSQGHA CGAINVPYMN
    60 70 80 90 100
    RGASGMSKNP DFLEQVSSHF GQSDNIIVGC QSGGRSIKAT TDLLHAGFTG
    110 120
    VKDIVGGYSA WAKNGLPTKA
    Length:120
    Mass (Da):12,676
    Last modified:February 1, 2005 - v2
    Checksum:i81CAE9D3C2DFCAFE
    GO

    Sequence cautioni

    The sequence CAA61433 differs from that shown. Reason: Frameshift at positions 40, 43 and 116.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti3E → D in CAA61433 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X89036 mRNA. Translation: CAA61433.1. Frameshift.
    AB011474 Genomic DNA. Translation: BAB10409.1.
    CP002688 Genomic DNA. Translation: AED98146.1.
    AY049302 mRNA. Translation: AAK83644.1.
    BT000864 mRNA. Translation: AAN38701.1.
    AY084763 mRNA. Translation: AAM61332.1.
    PIRiS58275.
    RefSeqiNP_851278.1. NM_180947.3. [Q39129-1]
    UniGeneiAt.23333.

    Genome annotation databases

    EnsemblPlantsiAT5G66040.1; AT5G66040.1; AT5G66040. [Q39129-1]
    GeneIDi836734.
    GrameneiAT5G66040.1; AT5G66040.1; AT5G66040.
    KEGGiath:AT5G66040.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X89036 mRNA. Translation: CAA61433.1. Frameshift.
    AB011474 Genomic DNA. Translation: BAB10409.1.
    CP002688 Genomic DNA. Translation: AED98146.1.
    AY049302 mRNA. Translation: AAK83644.1.
    BT000864 mRNA. Translation: AAN38701.1.
    AY084763 mRNA. Translation: AAM61332.1.
    PIRiS58275.
    RefSeqiNP_851278.1. NM_180947.3. [Q39129-1]
    UniGeneiAt.23333.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1TQ1NMR-A2-120[»]
    ProteinModelPortaliQ39129.
    SMRiQ39129.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi21976. 1 interactor.
    MINTiMINT-8071292.
    STRINGi3702.AT5G66040.1.

    Proteomic databases

    PaxDbiQ39129.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G66040.1; AT5G66040.1; AT5G66040. [Q39129-1]
    GeneIDi836734.
    GrameneiAT5G66040.1; AT5G66040.1; AT5G66040.
    KEGGiath:AT5G66040.

    Organism-specific databases

    TAIRiAT5G66040.

    Phylogenomic databases

    eggNOGiKOG1530. Eukaryota.
    COG0607. LUCA.
    HOGENOMiHOG000247776.
    InParanoidiQ39129.
    OMAiSAWRENE.
    OrthoDBiEOG09360OE7.
    PhylomeDBiQ39129.

    Enzyme and pathway databases

    BioCyciARA:AT5G66040-MONOMER.
    SABIO-RKQ39129.

    Miscellaneous databases

    EvolutionaryTraceiQ39129.
    PROiQ39129.

    Gene expression databases

    ExpressionAtlasiQ39129. baseline and differential.
    GenevisibleiQ39129. AT.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSTR16_ARATH
    AccessioniPrimary (citable) accession number: Q39129
    Secondary accession number(s): Q9FKX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: February 1, 2005
    Last modified: November 30, 2016
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.