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Protein

Vacuolar-processing enzyme gamma-isozyme

Gene

At4g32940

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Asparagine-specific endopeptidase involved in the processing of vacuolar seed protein precursors into the mature forms (PubMed:10417725). Probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:14688293).2 Publications

Catalytic activityi

Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa-bonds.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei177Sequence analysis1
Active sitei219Sequence analysis1
Sitei266Required for post-translational maturation and enzyme activityBy similarity1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: TAIR
  • endopeptidase activity Source: UniProtKB

GO - Biological processi

  • leaf senescence Source: UniProtKB
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • response to ethylene Source: UniProtKB
  • response to jasmonic acid Source: UniProtKB
  • response to salicylic acid Source: UniProtKB
  • response to wounding Source: UniProtKB
  • vacuolar protein processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC13.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-processing enzyme gamma-isozyme (EC:3.4.22.34By similarity)
Alternative name(s):
Asparaginyl endopeptidase gamma-VPECurated
Gamma-VPE
Gene namesi
Ordered Locus Names:At4g32940
ORF Names:F26P21.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G32940.

Subcellular locationi

  • Vacuole 1 Publication
  • Lytic vacuole 1 Publication

  • Note: Localized in lytic vacuoles of wounded leaves.1 Publication

GO - Cellular componenti

  • lytic vacuole Source: TAIR
  • protein storage vacuole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Disruption phenotypei

No macroscopic phenotype, probably due to functional redundancy. In plants lacking all vacuolar-processing enzyme isozymes (e.g. alpha, beta, gamma and delta) shift of storage protein accumulation from normally processed polypeptides to a finite number of prominent alternatively processed polypeptides cleaved at sites other than the conserved Asn residues targeted by VPE.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000002652830 – 494Vacuolar-processing enzyme gamma-isozymeAdd BLAST465

Post-translational modificationi

Auto-catalytic activation.By similarity

Proteomic databases

PaxDbiQ39119.

Expressioni

Tissue specificityi

Specific to vegetative organs, especially in senescent tissues. Also expressed in seeds and root tips.2 Publications

Inductioni

By senescence, wounding, jasmonic acid (JA), ethylene and salicylic acid (SA).1 Publication

Gene expression databases

GenevisibleiQ39119. AT.

Interactioni

Protein-protein interaction databases

BioGridi14716. 1 interactor.
STRINGi3702.AT4G32940.1.

Structurei

3D structure databases

ProteinModelPortaliQ39119.
SMRiQ39119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1348. Eukaryota.
COG5206. LUCA.
HOGENOMiHOG000236335.
InParanoidiQ39119.
KOiK01369.
OMAiPANDNYT.
OrthoDBiEOG093608MJ.
PhylomeDBiQ39119.

Family and domain databases

InterProiIPR001096. Peptidase_C13.
[Graphical view]
PANTHERiPTHR12000. PTHR12000. 1 hit.
PfamiPF01650. Peptidase_C13. 1 hit.
[Graphical view]
PIRSFiPIRSF019663. Legumain. 1 hit.
PRINTSiPR00776. HEMOGLOBNASE.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTMTRVSV GVVLFVLLVS LVAVSAARSG PDDVIKLPSQ ASRFFRPAEN
60 70 80 90 100
DDDSNSGTRW AVLVAGSSGY WNYRHQADIC HAYQLLRKGG LKEENIVVFM
110 120 130 140 150
YDDIANNYEN PRPGTIINSP HGKDVYQGVP KDYTGDDVNV DNLFAVILGD
160 170 180 190 200
KTAVKGGSGK VVDSGPNDHI FIFYSDHGGP GVLGMPTSPY LYANDLNDVL
210 220 230 240 250
KKKHALGTYK SLVFYLEACE SGSIFEGLLP EGLNIYATTA SNAEESSWGT
260 270 280 290 300
YCPGEEPSPP PEYETCLGDL YSVAWMEDSG MHNLQTETLH QQYELVKRRT
310 320 330 340 350
APVGYSYGSH VMQYGDVGIS KDNLDLYMGT NPANDNFTFA DANSLKPPSR
360 370 380 390 400
VTNQRDADLV HFWEKYRKAP EGSARKTEAQ KQVLEAMSHR LHIDNSVILV
410 420 430 440 450
GKILFGISRG PEVLNKVRSA GQPLVDDWNC LKNQVRAFER HCGSLSQYGI
460 470 480 490
KHMRSFANIC NAGIQMEQME EAASQACTTL PTGPWSSLNR GFSA
Length:494
Mass (Da):54,336
Last modified:May 29, 2007 - v2
Checksum:i9C8C47E644490984
GO

Sequence cautioni

The sequence BAA18924 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D61395 Genomic DNA. Translation: BAA18924.1. Different initiation.
AL031804 Genomic DNA. Translation: CAA21203.1.
AL161582 Genomic DNA. Translation: CAB80011.1.
CP002687 Genomic DNA. Translation: AEE86150.1.
AF370160 mRNA. Translation: AAK43975.1.
AF424619 mRNA. Translation: AAL11612.1.
AY059104 mRNA. Translation: AAL15210.1.
AY133531 mRNA. Translation: AAM91361.1.
PIRiT05302.
RefSeqiNP_195020.1. NM_119448.4.
UniGeneiAt.21567.
At.48929.
At.75634.

Genome annotation databases

EnsemblPlantsiAT4G32940.1; AT4G32940.1; AT4G32940.
GeneIDi829431.
GrameneiAT4G32940.1; AT4G32940.1; AT4G32940.
KEGGiath:AT4G32940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D61395 Genomic DNA. Translation: BAA18924.1. Different initiation.
AL031804 Genomic DNA. Translation: CAA21203.1.
AL161582 Genomic DNA. Translation: CAB80011.1.
CP002687 Genomic DNA. Translation: AEE86150.1.
AF370160 mRNA. Translation: AAK43975.1.
AF424619 mRNA. Translation: AAL11612.1.
AY059104 mRNA. Translation: AAL15210.1.
AY133531 mRNA. Translation: AAM91361.1.
PIRiT05302.
RefSeqiNP_195020.1. NM_119448.4.
UniGeneiAt.21567.
At.48929.
At.75634.

3D structure databases

ProteinModelPortaliQ39119.
SMRiQ39119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14716. 1 interactor.
STRINGi3702.AT4G32940.1.

Protein family/group databases

MEROPSiC13.006.

Proteomic databases

PaxDbiQ39119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G32940.1; AT4G32940.1; AT4G32940.
GeneIDi829431.
GrameneiAT4G32940.1; AT4G32940.1; AT4G32940.
KEGGiath:AT4G32940.

Organism-specific databases

TAIRiAT4G32940.

Phylogenomic databases

eggNOGiKOG1348. Eukaryota.
COG5206. LUCA.
HOGENOMiHOG000236335.
InParanoidiQ39119.
KOiK01369.
OMAiPANDNYT.
OrthoDBiEOG093608MJ.
PhylomeDBiQ39119.

Miscellaneous databases

PROiQ39119.

Gene expression databases

GenevisibleiQ39119. AT.

Family and domain databases

InterProiIPR001096. Peptidase_C13.
[Graphical view]
PANTHERiPTHR12000. PTHR12000. 1 hit.
PfamiPF01650. Peptidase_C13. 1 hit.
[Graphical view]
PIRSFiPIRSF019663. Legumain. 1 hit.
PRINTSiPR00776. HEMOGLOBNASE.
ProtoNetiSearch...

Entry informationi

Entry nameiVPEG_ARATH
AccessioniPrimary (citable) accession number: Q39119
Secondary accession number(s): Q93VM0, Q9SB73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: May 29, 2007
Last modified: November 30, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.