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Protein

Gibberellin 20 oxidase 2

Gene

GA20OX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. GA53 is less effectively oxidized than GA12, and GA25 is also formed as a minor product. Involved in the promotion of the floral transition, fertiliy and silique elongation, but plays only a minor role in elongation of seedling organs. Acts redundantly with GA20OX1.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: gibberellin biosynthesis

This protein is involved in the pathway gibberellin biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gibberellin biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi245 – 2451IronPROSITE-ProRule annotation
Metal bindingi247 – 2471IronPROSITE-ProRule annotation
Metal bindingi301 – 3011IronPROSITE-ProRule annotation
Active sitei311 – 3111Sequence analysis

GO - Molecular functioni

  • gibberellin 20-oxidase activity Source: TAIR
  • iron ion binding Source: InterPro

GO - Biological processi

  • flower development Source: TAIR
  • gibberellic acid mediated signaling pathway Source: GO_Central
  • gibberellin biosynthetic process Source: TAIR
  • response to gibberellin Source: TAIR
  • response to karrikin Source: TAIR
  • response to red or far red light Source: TAIR
  • unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G51810-MONOMER.
MetaCyc:AT5G51810-MONOMER.
BRENDAi1.14.11.12. 399.
UniPathwayiUPA00390.

Names & Taxonomyi

Protein namesi
Recommended name:
Gibberellin 20 oxidase 2 (EC:1.14.11.-)
Alternative name(s):
GA 20-oxidase 2
Gibberellin C-20 oxidase 2
Gene namesi
Name:GA20OX2
Synonyms:20ox, At2353
Ordered Locus Names:At5g51810
ORF Names:MIO24.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G51810.

Pathology & Biotechi

Disruption phenotypei

Slightly smaller than the wild type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Gibberellin 20 oxidase 2PRO_0000219515Add
BLAST

Proteomic databases

PaxDbiQ39111.
PRIDEiQ39111.

Expressioni

Tissue specificityi

Expressed in inflorescence and developing siliques. Detected in seeds, roots, cotyledons and leaves. In seeds, specifically detected at the rim of the embryo and the outer integument.4 Publications

Developmental stagei

Expressed in developing siliques 3-13 days after pollination.1 Publication

Inductioni

Negatively controlled by the level of physiologically active gibberellin. Up-regulated by auxin, paclobutrazol, long day exposure and cold treatment.4 Publications

Gene expression databases

GenevisibleiQ39111. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G51810.1.

Structurei

3D structure databases

ProteinModelPortaliQ39111.
SMRiQ39111. Positions 42-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini220 – 320101Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
HOGENOMiHOG000276735.
InParanoidiQ39111.
KOiK05282.
OMAiIDIPELN.
OrthoDBiEOG09360BJH.
PhylomeDBiQ39111.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILCTTTSP AEKEHEPKQD LEKDQTSPLI FNPSLLNLQS QIPNQFIWPD
60 70 80 90 100
EEKPSIDIPE LNVPFIDLSS QDSTLEAPRV IAEACTKHGF FLVVNHGVSE
110 120 130 140 150
SLIADAHRLM ESFFDMPLAG KQKAQRKPGE SCGYASSFTG RFSTKLPWKE
160 170 180 190 200
TLSFQFSNDN SGSRTVQDYF SDTLGQEFEQ FGKVYQDYCE AMSSLSLKIM
210 220 230 240 250
ELLGLSLGVN RDYFRGFFEE NDSIMRLNHY PPCQTPDLTL GTGPHCDPSS
260 270 280 290 300
LTILHQDHVN GLQVFVDNQW QSIRPNPKAF VVNIGDTFMA LSNGIFKSCL
310 320 330 340 350
HRAVVNRESA RKSMAFFLCP KKDKVVKPPS DILEKMKTRK YPDFTWSMFL
360 370
EFTQKHYRAD VNTLDSFSNW VITNNNPI
Length:378
Mass (Da):42,937
Last modified:November 1, 1996 - v1
Checksum:i0B5CF4E6FDBD2B74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83380 mRNA. Translation: CAA58294.1.
AB010074 Genomic DNA. Translation: BAB11250.1.
CP002688 Genomic DNA. Translation: AED96129.1.
RefSeqiNP_199994.1. NM_124560.3.
UniGeneiAt.28053.

Genome annotation databases

EnsemblPlantsiAT5G51810.1; AT5G51810.1; AT5G51810.
GeneIDi835256.
GrameneiAT5G51810.1; AT5G51810.1; AT5G51810.
KEGGiath:AT5G51810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83380 mRNA. Translation: CAA58294.1.
AB010074 Genomic DNA. Translation: BAB11250.1.
CP002688 Genomic DNA. Translation: AED96129.1.
RefSeqiNP_199994.1. NM_124560.3.
UniGeneiAt.28053.

3D structure databases

ProteinModelPortaliQ39111.
SMRiQ39111. Positions 42-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G51810.1.

Proteomic databases

PaxDbiQ39111.
PRIDEiQ39111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G51810.1; AT5G51810.1; AT5G51810.
GeneIDi835256.
GrameneiAT5G51810.1; AT5G51810.1; AT5G51810.
KEGGiath:AT5G51810.

Organism-specific databases

TAIRiAT5G51810.

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
HOGENOMiHOG000276735.
InParanoidiQ39111.
KOiK05282.
OMAiIDIPELN.
OrthoDBiEOG09360BJH.
PhylomeDBiQ39111.

Enzyme and pathway databases

UniPathwayiUPA00390.
BioCyciARA:AT5G51810-MONOMER.
MetaCyc:AT5G51810-MONOMER.
BRENDAi1.14.11.12. 399.

Miscellaneous databases

PROiQ39111.

Gene expression databases

GenevisibleiQ39111. AT.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAOX2_ARATH
AccessioniPrimary (citable) accession number: Q39111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.