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Protein

Gibberellin 20 oxidase 1

Gene

GA20OX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. GA53 is less effectively oxidized than GA12, and GA25 is also formed as a minor product. Involved in the promotion of the floral transition, fertility and silique elongation, but plays only a minor role in elongation of seedling organs. Acts redundantly with GA20OX2.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: gibberellin biosynthesis

This protein is involved in the pathway gibberellin biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gibberellin biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi247IronPROSITE-ProRule annotation1
Metal bindingi249IronPROSITE-ProRule annotation1
Metal bindingi303IronPROSITE-ProRule annotation1
Active sitei313Sequence analysis1

GO - Molecular functioni

  • gibberellin 20-oxidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • flower development Source: TAIR
  • gibberellic acid mediated signaling pathway Source: TAIR
  • gibberellin biosynthetic process Source: TAIR
  • leaf development Source: TAIR
  • response to gibberellin Source: TAIR
  • short-day photoperiodism, flowering Source: TAIR
  • unidimensional cell growth Source: TAIR

Keywordsi

Molecular functionOxidoreductase
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G25420-MONOMER.
MetaCyc:AT4G25420-MONOMER.
UniPathwayiUPA00390.

Names & Taxonomyi

Protein namesi
Recommended name:
Gibberellin 20 oxidase 1 (EC:1.14.11.-)
Alternative name(s):
GA 20-oxidase 1
Short name:
AtGA20ox1
Gibberellin C-20 oxidase 1
Gene namesi
Name:GA20OX1
Synonyms:20ox1, At2301, GA5
Ordered Locus Names:At4g25420
ORF Names:T30C3.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G25420.
TAIRilocus:2005511. AT4G25420.

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Semi-dwarf.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002195141 – 377Gibberellin 20 oxidase 1Add BLAST377

Proteomic databases

PaxDbiQ39110.

Expressioni

Tissue specificityi

Highly expressed in stems and inflorescence tissues. Detected in seeds, roots, leaves and siliques.2 Publications

Inductioni

Circadian-regulation. Up-regulated by auxin, paclobutrazol and cold treatment. Negatively controlled by the level of physiologically active gibberellin.5 Publications

Gene expression databases

ExpressionAtlasiQ39110. baseline and differential.
GenevisibleiQ39110. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G25420.1.

Structurei

3D structure databases

ProteinModelPortaliQ39110.
SMRiQ39110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 322Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
HOGENOMiHOG000276735.
InParanoidiQ39110.
KOiK05282.
OMAiLISEACY.
OrthoDBiEOG09360BJH.
PhylomeDBiQ39110.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiView protein in InterPro
IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
PROSITEiView protein in PROSITE
PS51471. FE2OG_OXY. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSFVTTSP EEEDKPKLGL GNIQTPLIFN PSMLNLQANI PNQFIWPDDE
60 70 80 90 100
KPSINVLELD VPLIDLQNLL SDPSSTLDAS RLISEACKKH GFFLVVNHGI
110 120 130 140 150
SEELISDAHE YTSRFFDMPL SEKQRVLRKS GESVGYASSF TGRFSTKLPW
160 170 180 190 200
KETLSFRFCD DMSRSKSVQD YFCDALGHGF QPFGKVYQEY CEAMSSLSLK
210 220 230 240 250
IMELLGLSLG VKRDYFREFF EENDSIMRLN YYPPCIKPDL TLGTGPHCDP
260 270 280 290 300
TSLTILHQDH VNGLQVFVEN QWRSIRPNPK AFVVNIGDTF MALSNDRYKS
310 320 330 340 350
CLHRAVVNSE SERKSLAFFL CPKKDRVVTP PRELLDSITS RRYPDFTWSM
360 370
FLEFTQKHYR ADMNTLQAFS DWLTKPI
Length:377
Mass (Da):43,224
Last modified:March 1, 2005 - v2
Checksum:i35F4EAD40AE67E7F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7T → R in AAC39314 (PubMed:7604047).Curated1
Sequence conflicti35N → D in AAC39314 (PubMed:7604047).Curated1
Sequence conflicti40 – 41IP → MA in AAC39314 (PubMed:7604047).Curated2
Sequence conflicti45I → H in AAC39314 (PubMed:7604047).Curated1
Sequence conflicti54 – 56INV → TLQ in AAC39314 (PubMed:7604047).Curated3
Sequence conflicti236I → M in AAA76864 (PubMed:7604047).Curated1
Sequence conflicti236I → M in AAC39313 (PubMed:7604047).Curated1
Sequence conflicti311 – 312SE → RM in AAC39314 (PubMed:7604047).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti272 – 377Missing in strain: cv. Landsberg erecta; allele ga5; semidwarfing. 1 PublicationAdd BLAST106
Natural varianti310E → K in strain: cv Landsberg erecta. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83379 mRNA. Translation: CAA58293.1.
U20872 Genomic DNA. Translation: AAC39313.2.
U20873 Genomic DNA. Translation: AAC39314.2.
U20901 Genomic DNA. Translation: AAA76864.2.
AL079350 Genomic DNA. Translation: CAB45519.1.
AL161563 Genomic DNA. Translation: CAB81353.1.
CP002687 Genomic DNA. Translation: AEE85055.1.
PIRiT10222.
RefSeqiNP_194272.1. NM_118674.5.
UniGeneiAt.32258.

Genome annotation databases

EnsemblPlantsiAT4G25420.1; AT4G25420.1; AT4G25420.
GeneIDi828645.
GrameneiAT4G25420.1; AT4G25420.1; AT4G25420.
KEGGiath:AT4G25420.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGAOX1_ARATH
AccessioniPrimary (citable) accession number: Q39110
Secondary accession number(s): Q38844
, Q9FYN4, Q9FYN5, Q9STJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: March 28, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome