Q39099 (XTH4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 4
Short name=At-XTH4
Short name=XTH-4
EC=2.4.1.207

Gene names

Name:	XTH4
Synonyms:	EXGT-A1, EXT
Ordered Locus Names:	At2g06850
ORF Names:	T9F8.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan. Ref.8
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Predominantly expressed in young developing tissues. Expressed in 7 day old seedlings, roots, nodes bearing flowers, flower buds and siliques. Ref.2
Induction	By auxin and brassinolide. Ref.7 Ref.9
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity. N-glycosylated; not essential for its enzymatic activity. Ref.8
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.
Sequence caution	The sequence AAK62373.1 differs from that shown. Reason: Frameshift at position 146.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro response to auxin stimulus Inferred from expression pattern Ref.7. Source: TAIR response to low light intensity stimulus Inferred from expression pattern Ref.7. Source: TAIR response to mechanical stimulus Inferred from expression pattern Ref.7. Source: TAIR unidimensional cell growth Inferred from mutant phenotype Ref.2. Source: TAIR
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast Inferred from direct assay PubMed 15028209. Source: TAIR extracellular region Inferred from direct assay PubMed 1400418. Source: TAIR membrane Inferred from direct assay PubMed 17432890. Source: TAIR plant-type cell wall Inferred from direct assay PubMed 16287169PubMed 17526915. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from mutant phenotype PubMed 1400418. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 296	272	Xyloglucan endotransglucosylase/hydrolase protein 4		PRO_0000011804
Sites
	Active site	109	1	Nucleophile By similarity
	Active site	113	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	117	1	N-linked (GlcNAc...) Probable
Experimental info
	Sequence conflict	205 – 206	2	TR → PK in AAK62373. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q39099 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 455F3574BE7751CC	FASTA	296	34,291
	10 20 30 40 50 60 MTVSSSPWAL MALFLMVSST MVMAIPPRKA IDVPFGRNYV PTWAFDHQKQ FNGGSELQLI 70 80 90 100 110 120 LDKYTGTGFQ SKGSYLFGHF SMHIKLPAGD TAGVVTAFYL SSTNNEHDEI DFEFLGNRTG 130 140 150 160 170 180 QPAILQTNVF TGGKGNREQR IYLWFDPSKA YHTYSILWNM YQIVFFVDNI PIRTFKNAKD 190 200 210 220 230 240 LGVRFPFNQP MKLYSSLWNA DDWATRGGLE KTNWANAPFV ASYKGFHIDG CQASVEAKYC 250 260 270 280 290 ATQGRMWWDQ KEFRDLDAEQ WRRLKWVRMK WTIYNYCTDR TRFPVMPAEC KRDRDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls." Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K. J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis." Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y. Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression." Xu W., Campbell P., Vargheese A.K., Braam J. Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[8]	"In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis." Campbell P., Braam J. Plant J. 18:371-382(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
[9]	"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis." Yokoyama R., Nishitani K. Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[10]	"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature." Rose J.K.C., Braam J., Fry S.C., Nishitani K. Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

XTH-World

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16454 mRNA. Translation: BAA03921.1. AF163819 Genomic DNA. Translation: AAD45123.1. AC005561 Genomic DNA. Translation: AAC98464.1. CP002685 Genomic DNA. Translation: AEC06021.1. AF386928 mRNA. Translation: AAK62373.1. Frameshift. AY054547 mRNA. Translation: AAK96738.1. AY056201 mRNA. Translation: AAL07050.1. AY059873 mRNA. Translation: AAL24355.1. AY064672 mRNA. Translation: AAL47378.1. AY114644 mRNA. Translation: AAM47963.1. AY085465 mRNA. Translation: AAM62691.1.
IPI	IPI00528839.
PIR	C49539.
RefSeq	NP_178708.1. NM_126666.3.
UniGene	At.24328. At.74042.
3D structure databases
ProteinModelPortal	Q39099.
SMR	Q39099. Positions 31-295.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Proteomic databases
PaxDb	Q39099.
PRIDE	Q39099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G06850.1; AT2G06850.1; AT2G06850.
GeneID	815247.
KEGG	ath:AT2G06850.
Organism-specific databases
TAIR	At2g06850.
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HOG000236368.
InParanoid	Q39099.
KO	K08235.
OMA	QGARWWD.
PhylomeDB	Q39099.
ProtClustDB	CLSN2683385.
Gene expression databases
ArrayExpress	Q39099.
Genevestigator	Q39099.
GermOnline	AT2G06850. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008264. Beta_glucanase. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
PRINTS	PR00737. GLHYDRLASE16.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH4_ARATH

Accession

Primary (citable) accession number: Q39099
Secondary accession number(s): Q94F33

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program