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Q39099

- XTH4_ARATH

UniProt

Q39099 - XTH4_ARATH

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Protein
Xyloglucan endotransglucosylase/hydrolase protein 4
Gene
XTH4, EXGT-A1, EXT, At2g06850, T9F8.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Nucleophile By similarity
Active sitei113 – 1131Proton donor By similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. response to auxin Source: TAIR
  3. response to low light intensity stimulus Source: TAIR
  4. response to mechanical stimulus Source: TAIR
  5. unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G06850-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 4 (EC:2.4.1.207)
Short name:
At-XTH4
Short name:
XTH-4
Gene namesi
Name:XTH4
Synonyms:EXGT-A1, EXT
Ordered Locus Names:At2g06850
ORF Names:T9F8.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G06850.

Subcellular locationi

Secretedcell wall Inferred. Secretedextracellular spaceapoplast Inferred

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: TAIR
  3. chloroplast Source: TAIR
  4. cytoplasm Source: TAIR
  5. extracellular region Source: TAIR
  6. membrane Source: TAIR
  7. plant-type cell wall Source: TAIR
  8. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 296272Xyloglucan endotransglucosylase/hydrolase protein 4
PRO_0000011804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi117 – 1171N-linked (GlcNAc...) Inferred

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
N-glycosylated; not essential for its enzymatic activity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ39099.
PRIDEiQ39099.

Expressioni

Tissue specificityi

Predominantly expressed in young developing tissues. Expressed in 7 day old seedlings, roots, nodes bearing flowers, flower buds and siliques.1 Publication

Inductioni

By auxin and brassinolide.2 Publications

Gene expression databases

ArrayExpressiQ39099.
GenevestigatoriQ39099.

Interactioni

Protein-protein interaction databases

BioGridi640. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ39099.
SMRiQ39099. Positions 31-295.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ39099.
KOiK08235.
OMAiNWANAPF.
PhylomeDBiQ39099.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39099-1 [UniParc]FASTAAdd to Basket

« Hide

MTVSSSPWAL MALFLMVSST MVMAIPPRKA IDVPFGRNYV PTWAFDHQKQ    50
FNGGSELQLI LDKYTGTGFQ SKGSYLFGHF SMHIKLPAGD TAGVVTAFYL 100
SSTNNEHDEI DFEFLGNRTG QPAILQTNVF TGGKGNREQR IYLWFDPSKA 150
YHTYSILWNM YQIVFFVDNI PIRTFKNAKD LGVRFPFNQP MKLYSSLWNA 200
DDWATRGGLE KTNWANAPFV ASYKGFHIDG CQASVEAKYC ATQGRMWWDQ 250
KEFRDLDAEQ WRRLKWVRMK WTIYNYCTDR TRFPVMPAEC KRDRDA 296
Length:296
Mass (Da):34,291
Last modified:November 1, 1996 - v1
Checksum:i455F3574BE7751CC
GO

Sequence cautioni

The sequence AAK62373.1 differs from that shown. Reason: Frameshift at position 146.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti205 – 2062TR → PK in AAK62373. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16454 mRNA. Translation: BAA03921.1.
AF163819 Genomic DNA. Translation: AAD45123.1.
AC005561 Genomic DNA. Translation: AAC98464.1.
CP002685 Genomic DNA. Translation: AEC06021.1.
AF386928 mRNA. Translation: AAK62373.1. Frameshift.
AY054547 mRNA. Translation: AAK96738.1.
AY056201 mRNA. Translation: AAL07050.1.
AY059873 mRNA. Translation: AAL24355.1.
AY064672 mRNA. Translation: AAL47378.1.
AY114644 mRNA. Translation: AAM47963.1.
AY085465 mRNA. Translation: AAM62691.1.
PIRiC49539.
RefSeqiNP_178708.1. NM_126666.3.
UniGeneiAt.24328.
At.74042.

Genome annotation databases

EnsemblPlantsiAT2G06850.1; AT2G06850.1; AT2G06850.
GeneIDi815247.
KEGGiath:AT2G06850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16454 mRNA. Translation: BAA03921.1 .
AF163819 Genomic DNA. Translation: AAD45123.1 .
AC005561 Genomic DNA. Translation: AAC98464.1 .
CP002685 Genomic DNA. Translation: AEC06021.1 .
AF386928 mRNA. Translation: AAK62373.1 . Frameshift.
AY054547 mRNA. Translation: AAK96738.1 .
AY056201 mRNA. Translation: AAL07050.1 .
AY059873 mRNA. Translation: AAL24355.1 .
AY064672 mRNA. Translation: AAL47378.1 .
AY114644 mRNA. Translation: AAM47963.1 .
AY085465 mRNA. Translation: AAM62691.1 .
PIRi C49539.
RefSeqi NP_178708.1. NM_126666.3.
UniGenei At.24328.
At.74042.

3D structure databases

ProteinModelPortali Q39099.
SMRi Q39099. Positions 31-295.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 640. 5 interactions.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi Q39099.
PRIDEi Q39099.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G06850.1 ; AT2G06850.1 ; AT2G06850 .
GeneIDi 815247.
KEGGi ath:AT2G06850.

Organism-specific databases

TAIRi AT2G06850.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000236368.
InParanoidi Q39099.
KOi K08235.
OMAi NWANAPF.
PhylomeDBi Q39099.

Enzyme and pathway databases

BioCyci ARA:AT2G06850-MONOMER.

Gene expression databases

ArrayExpressi Q39099.
Genevestigatori Q39099.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
PRINTSi PR00737. GLHYDRLASE16.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
    Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
    J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
    Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
    Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
    Xu W., Campbell P., Vargheese A.K., Braam J.
    Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis."
    Campbell P., Braam J.
    Plant J. 18:371-382(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
  9. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH4_ARATH
AccessioniPrimary (citable) accession number: Q39099
Secondary accession number(s): Q94F33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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