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Protein

Xyloglucan endotransglucosylase/hydrolase protein 4

Gene

XTH4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei109NucleophilePROSITE-ProRule annotation1
Active sitei113Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

  • hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  • xyloglucan:xyloglucosyl transferase activity Source: TAIR

GO - Biological processi

  • cell wall biogenesis Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • response to auxin Source: TAIR
  • response to low light intensity stimulus Source: TAIR
  • response to mechanical stimulus Source: TAIR
  • unidimensional cell growth Source: TAIR
  • xyloglucan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G06850-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 4 (EC:2.4.1.207)
Short name:
At-XTH4
Short name:
XTH-4
Gene namesi
Name:XTH4
Synonyms:EXGT-A1, EXT
Ordered Locus Names:At2g06850
ORF Names:T9F8.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G06850.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB-SubCell
  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • cytoplasm Source: TAIR
  • extracellular region Source: TAIR
  • membrane Source: TAIR
  • plant-type cell wall Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001180425 – 296Xyloglucan endotransglucosylase/hydrolase protein 4Add BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi117N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
N-glycosylated; not essential for its enzymatic activity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ39099.
PRIDEiQ39099.

Expressioni

Tissue specificityi

Predominantly expressed in young developing tissues. Expressed in 7 day old seedlings, roots, nodes bearing flowers, flower buds and siliques.1 Publication

Inductioni

By auxin and brassinolide.2 Publications

Gene expression databases

GenevisibleiQ39099. AT.

Interactioni

Protein-protein interaction databases

BioGridi640. 5 interactors.
STRINGi3702.AT2G06850.1.

Structurei

3D structure databases

ProteinModelPortaliQ39099.
SMRiQ39099.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 223GH16PROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JVP5. Eukaryota.
ENOG41105R2. LUCA.
HOGENOMiHOG000236368.
InParanoidiQ39099.
KOiK08235.
OMAiEKTNWAN.
OrthoDBiEOG09360HPS.
PhylomeDBiQ39099.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVSSSPWAL MALFLMVSST MVMAIPPRKA IDVPFGRNYV PTWAFDHQKQ
60 70 80 90 100
FNGGSELQLI LDKYTGTGFQ SKGSYLFGHF SMHIKLPAGD TAGVVTAFYL
110 120 130 140 150
SSTNNEHDEI DFEFLGNRTG QPAILQTNVF TGGKGNREQR IYLWFDPSKA
160 170 180 190 200
YHTYSILWNM YQIVFFVDNI PIRTFKNAKD LGVRFPFNQP MKLYSSLWNA
210 220 230 240 250
DDWATRGGLE KTNWANAPFV ASYKGFHIDG CQASVEAKYC ATQGRMWWDQ
260 270 280 290
KEFRDLDAEQ WRRLKWVRMK WTIYNYCTDR TRFPVMPAEC KRDRDA
Length:296
Mass (Da):34,291
Last modified:November 1, 1996 - v1
Checksum:i455F3574BE7751CC
GO

Sequence cautioni

The sequence AAK62373 differs from that shown. Reason: Frameshift at position 146.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti205 – 206TR → PK in AAK62373 (PubMed:14593172).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16454 mRNA. Translation: BAA03921.1.
AF163819 Genomic DNA. Translation: AAD45123.1.
AC005561 Genomic DNA. Translation: AAC98464.1.
CP002685 Genomic DNA. Translation: AEC06021.1.
AF386928 mRNA. Translation: AAK62373.1. Frameshift.
AY054547 mRNA. Translation: AAK96738.1.
AY056201 mRNA. Translation: AAL07050.1.
AY059873 mRNA. Translation: AAL24355.1.
AY064672 mRNA. Translation: AAL47378.1.
AY114644 mRNA. Translation: AAM47963.1.
AY085465 mRNA. Translation: AAM62691.1.
PIRiC49539.
RefSeqiNP_178708.1. NM_126666.4.
UniGeneiAt.24328.
At.74042.

Genome annotation databases

EnsemblPlantsiAT2G06850.1; AT2G06850.1; AT2G06850.
GeneIDi815247.
GrameneiAT2G06850.1; AT2G06850.1; AT2G06850.
KEGGiath:AT2G06850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16454 mRNA. Translation: BAA03921.1.
AF163819 Genomic DNA. Translation: AAD45123.1.
AC005561 Genomic DNA. Translation: AAC98464.1.
CP002685 Genomic DNA. Translation: AEC06021.1.
AF386928 mRNA. Translation: AAK62373.1. Frameshift.
AY054547 mRNA. Translation: AAK96738.1.
AY056201 mRNA. Translation: AAL07050.1.
AY059873 mRNA. Translation: AAL24355.1.
AY064672 mRNA. Translation: AAL47378.1.
AY114644 mRNA. Translation: AAM47963.1.
AY085465 mRNA. Translation: AAM62691.1.
PIRiC49539.
RefSeqiNP_178708.1. NM_126666.4.
UniGeneiAt.24328.
At.74042.

3D structure databases

ProteinModelPortaliQ39099.
SMRiQ39099.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi640. 5 interactors.
STRINGi3702.AT2G06850.1.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbiQ39099.
PRIDEiQ39099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G06850.1; AT2G06850.1; AT2G06850.
GeneIDi815247.
GrameneiAT2G06850.1; AT2G06850.1; AT2G06850.
KEGGiath:AT2G06850.

Organism-specific databases

TAIRiAT2G06850.

Phylogenomic databases

eggNOGiENOG410JVP5. Eukaryota.
ENOG41105R2. LUCA.
HOGENOMiHOG000236368.
InParanoidiQ39099.
KOiK08235.
OMAiEKTNWAN.
OrthoDBiEOG09360HPS.
PhylomeDBiQ39099.

Enzyme and pathway databases

BioCyciARA:AT2G06850-MONOMER.

Miscellaneous databases

PROiQ39099.

Gene expression databases

GenevisibleiQ39099. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXTH4_ARATH
AccessioniPrimary (citable) accession number: Q39099
Secondary accession number(s): Q94F33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.