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Q39099

- XTH4_ARATH

UniProt

Q39099 - XTH4_ARATH

Protein

Xyloglucan endotransglucosylase/hydrolase protein 4

Gene

XTH4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei109 – 1091NucleophilePROSITE-ProRule annotation
    Active sitei113 – 1131Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro
    2. response to auxin Source: TAIR
    3. response to low light intensity stimulus Source: TAIR
    4. response to mechanical stimulus Source: TAIR
    5. unidimensional cell growth Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT2G06850-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase protein 4 (EC:2.4.1.207)
    Short name:
    At-XTH4
    Short name:
    XTH-4
    Gene namesi
    Name:XTH4
    Synonyms:EXGT-A1, EXT
    Ordered Locus Names:At2g06850
    ORF Names:T9F8.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G06850.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: TAIR
    3. chloroplast Source: TAIR
    4. cytoplasm Source: TAIR
    5. extracellular region Source: TAIR
    6. membrane Source: TAIR
    7. plant-type cell wall Source: TAIR
    8. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 296272Xyloglucan endotransglucosylase/hydrolase protein 4PRO_0000011804Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi117 – 1171N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
    N-glycosylated; not essential for its enzymatic activity.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ39099.
    PRIDEiQ39099.

    Expressioni

    Tissue specificityi

    Predominantly expressed in young developing tissues. Expressed in 7 day old seedlings, roots, nodes bearing flowers, flower buds and siliques.1 Publication

    Inductioni

    By auxin and brassinolide.2 Publications

    Gene expression databases

    ArrayExpressiQ39099.
    GenevestigatoriQ39099.

    Interactioni

    Protein-protein interaction databases

    BioGridi640. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39099.
    SMRiQ39099. Positions 31-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    InParanoidiQ39099.
    KOiK08235.
    OMAiNWANAPF.
    PhylomeDBiQ39099.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q39099-1 [UniParc]FASTAAdd to Basket

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    MTVSSSPWAL MALFLMVSST MVMAIPPRKA IDVPFGRNYV PTWAFDHQKQ    50
    FNGGSELQLI LDKYTGTGFQ SKGSYLFGHF SMHIKLPAGD TAGVVTAFYL 100
    SSTNNEHDEI DFEFLGNRTG QPAILQTNVF TGGKGNREQR IYLWFDPSKA 150
    YHTYSILWNM YQIVFFVDNI PIRTFKNAKD LGVRFPFNQP MKLYSSLWNA 200
    DDWATRGGLE KTNWANAPFV ASYKGFHIDG CQASVEAKYC ATQGRMWWDQ 250
    KEFRDLDAEQ WRRLKWVRMK WTIYNYCTDR TRFPVMPAEC KRDRDA 296
    Length:296
    Mass (Da):34,291
    Last modified:November 1, 1996 - v1
    Checksum:i455F3574BE7751CC
    GO

    Sequence cautioni

    The sequence AAK62373.1 differs from that shown. Reason: Frameshift at position 146.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti205 – 2062TR → PK in AAK62373. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16454 mRNA. Translation: BAA03921.1.
    AF163819 Genomic DNA. Translation: AAD45123.1.
    AC005561 Genomic DNA. Translation: AAC98464.1.
    CP002685 Genomic DNA. Translation: AEC06021.1.
    AF386928 mRNA. Translation: AAK62373.1. Frameshift.
    AY054547 mRNA. Translation: AAK96738.1.
    AY056201 mRNA. Translation: AAL07050.1.
    AY059873 mRNA. Translation: AAL24355.1.
    AY064672 mRNA. Translation: AAL47378.1.
    AY114644 mRNA. Translation: AAM47963.1.
    AY085465 mRNA. Translation: AAM62691.1.
    PIRiC49539.
    RefSeqiNP_178708.1. NM_126666.3.
    UniGeneiAt.24328.
    At.74042.

    Genome annotation databases

    EnsemblPlantsiAT2G06850.1; AT2G06850.1; AT2G06850.
    GeneIDi815247.
    KEGGiath:AT2G06850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16454 mRNA. Translation: BAA03921.1 .
    AF163819 Genomic DNA. Translation: AAD45123.1 .
    AC005561 Genomic DNA. Translation: AAC98464.1 .
    CP002685 Genomic DNA. Translation: AEC06021.1 .
    AF386928 mRNA. Translation: AAK62373.1 . Frameshift.
    AY054547 mRNA. Translation: AAK96738.1 .
    AY056201 mRNA. Translation: AAL07050.1 .
    AY059873 mRNA. Translation: AAL24355.1 .
    AY064672 mRNA. Translation: AAL47378.1 .
    AY114644 mRNA. Translation: AAM47963.1 .
    AY085465 mRNA. Translation: AAM62691.1 .
    PIRi C49539.
    RefSeqi NP_178708.1. NM_126666.3.
    UniGenei At.24328.
    At.74042.

    3D structure databases

    ProteinModelPortali Q39099.
    SMRi Q39099. Positions 31-295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 640. 5 interactions.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi Q39099.
    PRIDEi Q39099.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G06850.1 ; AT2G06850.1 ; AT2G06850 .
    GeneIDi 815247.
    KEGGi ath:AT2G06850.

    Organism-specific databases

    TAIRi AT2G06850.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    InParanoidi Q39099.
    KOi K08235.
    OMAi NWANAPF.
    PhylomeDBi Q39099.

    Enzyme and pathway databases

    BioCyci ARA:AT2G06850-MONOMER.

    Gene expression databases

    ArrayExpressi Q39099.
    Genevestigatori Q39099.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
      Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
      J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
      Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
      Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
      Xu W., Campbell P., Vargheese A.K., Braam J.
      Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis."
      Campbell P., Braam J.
      Plant J. 18:371-382(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
    9. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH4_ARATH
    AccessioniPrimary (citable) accession number: Q39099
    Secondary accession number(s): Q94F33
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3