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Q39099 (XTH4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xyloglucan endotransglucosylase/hydrolase protein 4

Short name=At-XTH4
Short name=XTH-4
EC=2.4.1.207
Gene names
Name:XTH4
Synonyms:EXGT-A1, EXT
Ordered Locus Names:At2g06850
ORF Names:T9F8.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan. Ref.8

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Predominantly expressed in young developing tissues. Expressed in 7 day old seedlings, roots, nodes bearing flowers, flower buds and siliques. Ref.2

Induction

By auxin and brassinolide. Ref.7 Ref.9

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

N-glycosylated; not essential for its enzymatic activity. Ref.8

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Sequence caution

The sequence AAK62373.1 differs from that shown. Reason: Frameshift at position 146.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentApoplast
Cell wall
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Transferase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular glucan metabolic process

Inferred from electronic annotation. Source: InterPro

response to auxin

Inferred from expression pattern Ref.7. Source: TAIR

response to low light intensity stimulus

Inferred from expression pattern Ref.7. Source: TAIR

response to mechanical stimulus

Inferred from expression pattern Ref.7. Source: TAIR

unidimensional cell growth

Inferred from mutant phenotype Ref.2. Source: TAIR

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from direct assay PubMed 15593128PubMed 15610358PubMed 16287169. Source: TAIR

chloroplast

Inferred from direct assay PubMed 15028209. Source: TAIR

cytoplasm

Inferred from direct assay PubMed 15610358. Source: TAIR

extracellular region

Inferred from direct assay PubMed 1400418. Source: TAIR

membrane

Inferred from direct assay PubMed 17432890. Source: TAIR

plant-type cell wall

Inferred from direct assay PubMed 16287169PubMed 17526915. Source: TAIR

plasmodesma

Inferred from direct assay PubMed 21533090. Source: TAIR

   Molecular_functionhydrolase activity, hydrolyzing O-glycosyl compounds

Inferred from electronic annotation. Source: InterPro

xyloglucan:xyloglucosyl transferase activity

Inferred from mutant phenotype PubMed 1400418. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 296272Xyloglucan endotransglucosylase/hydrolase protein 4
PRO_0000011804

Sites

Active site1091Nucleophile By similarity
Active site1131Proton donor By similarity

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Probable

Experimental info

Sequence conflict205 – 2062TR → PK in AAK62373. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q39099 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 455F3574BE7751CC

FASTA29634,291
        10         20         30         40         50         60 
MTVSSSPWAL MALFLMVSST MVMAIPPRKA IDVPFGRNYV PTWAFDHQKQ FNGGSELQLI 

        70         80         90        100        110        120 
LDKYTGTGFQ SKGSYLFGHF SMHIKLPAGD TAGVVTAFYL SSTNNEHDEI DFEFLGNRTG 

       130        140        150        160        170        180 
QPAILQTNVF TGGKGNREQR IYLWFDPSKA YHTYSILWNM YQIVFFVDNI PIRTFKNAKD 

       190        200        210        220        230        240 
LGVRFPFNQP MKLYSSLWNA DDWATRGGLE KTNWANAPFV ASYKGFHIDG CQASVEAKYC 

       250        260        270        280        290 
ATQGRMWWDQ KEFRDLDAEQ WRRLKWVRMK WTIYNYCTDR TRFPVMPAEC KRDRDA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
Xu W., Campbell P., Vargheese A.K., Braam J.
Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[8]"In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis."
Campbell P., Braam J.
Plant J. 18:371-382(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
[9]"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
Yokoyama R., Nishitani K.
Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[10]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16454 mRNA. Translation: BAA03921.1.
AF163819 Genomic DNA. Translation: AAD45123.1.
AC005561 Genomic DNA. Translation: AAC98464.1.
CP002685 Genomic DNA. Translation: AEC06021.1.
AF386928 mRNA. Translation: AAK62373.1. Frameshift.
AY054547 mRNA. Translation: AAK96738.1.
AY056201 mRNA. Translation: AAL07050.1.
AY059873 mRNA. Translation: AAL24355.1.
AY064672 mRNA. Translation: AAL47378.1.
AY114644 mRNA. Translation: AAM47963.1.
AY085465 mRNA. Translation: AAM62691.1.
PIRC49539.
RefSeqNP_178708.1. NM_126666.3.
UniGeneAt.24328.
At.74042.

3D structure databases

ProteinModelPortalQ39099.
SMRQ39099. Positions 31-295.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid640. 5 interactions.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbQ39099.
PRIDEQ39099.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G06850.1; AT2G06850.1; AT2G06850.
GeneID815247.
KEGGath:AT2G06850.

Organism-specific databases

TAIRAT2G06850.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ39099.
KOK08235.
OMALYMIAFF.
PhylomeDBQ39099.
ProtClustDBCLSN2683385.

Enzyme and pathway databases

BioCycARA:AT2G06850-MONOMER.

Gene expression databases

ArrayExpressQ39099.
GenevestigatorQ39099.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
PRINTSPR00737. GLHYDRLASE16.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH4_ARATH
AccessionPrimary (citable) accession number: Q39099
Secondary accession number(s): Q94F33
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names