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Protein

Transcription factor CAULIFLOWER

Gene

CAL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1, FRUITFULL and LEAFY. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Seems to be partially redundant to the function of APETALA1. Positively regulates the APETALA1 and LEAFY expression.2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • floral meristem determinacy Source: TAIR
  • positive regulation of flower development Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor CAULIFLOWER
Short name:
AtCAL
Alternative name(s):
Agamous-like MADS-box protein AGL10
Gene namesi
Name:CAL
Synonyms:AGL10
Ordered Locus Names:At1g26310
ORF Names:F28B23.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G26310.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271G → D in cal-3; no visible phenotype. 'Cauliflower' shape when associated with AP1 disruption. 1 Publication
Mutagenesisi39 – 391C → Y in cal-2; no visible phenotype. 'Cauliflower' shape when associated with AP1 disruption. 1 Publication
Mutagenesisi131 – 1311E → K in cal-4; no visible phenotype. 'Cauliflower' shape when associated with AP1 disruption. 1 Publication
Mutagenesisi151 – 255105Missing in cal-5; 'Cauliflower' shape. 1 PublicationAdd
BLAST
Mutagenesisi176 – 18611ERENILRTKQT → KRENILRTRQI in cal-1; no visible phenotype. 'Cauliflower' shape when associated with AP1 disruption. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Transcription factor CAULIFLOWERPRO_0000199480Add
BLAST

Proteomic databases

PaxDbiQ39081.
PRIDEiQ39081.

Expressioni

Tissue specificityi

Expressed in young flower primordia.

Developmental stagei

Expressed at an early stage of floral initiation.

Gene expression databases

GenevisibleiQ39081. AT.

Interactioni

Subunit structurei

Homodimer capable of binding to CArG-box sequences.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SOC1O646454EBI-592136,EBI-592041

Protein-protein interaction databases

BioGridi24408. 5 interactions.
DIPiDIP-33764N.
IntActiQ39081. 5 interactions.
STRINGi3702.AT1G26310.1.

Structurei

3D structure databases

ProteinModelPortaliQ39081.
SMRiQ39081. Positions 2-73, 96-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6161MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini90 – 18091K-boxPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili90 – 198109Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiQ39081.
KOiK09264.
OMAiLIAPDSH.
PhylomeDBiQ39081.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGRVELKR IENKINRQVT FSKRRTGLLK KAQEISVLCD AEVSLIVFSH
60 70 80 90 100
KGKLFEYSSE SCMEKVLERY ERYSYAERQL IAPDSHVNAQ TNWSMEYSRL
110 120 130 140 150
KAKIELLERN QRHYLGEELE PMSLKDLQNL EQQLETALKH IRSRKNQLMN
160 170 180 190 200
ESLNHLQRKE KEIQEENSML TKQIKERENI LRTKQTQCEQ LNRSVDDVPQ
210 220 230 240 250
PQPFQHPHLY MIAHQTSPFL NMGGLYQEED QTAMRRNNLD LTLEPIYNYL

GCYAA
Length:255
Mass (Da):30,187
Last modified:September 22, 2009 - v3
Checksum:i5F7313A804DF594D
GO

Sequence cautioni

The sequence AAA64789.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67505.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67506.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67507.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67508.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67509.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67510.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67511.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67512.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67513.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67514.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67515.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67516.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67517.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67518.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC67519.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAG50679.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti78 – 781R → S in strain: cv. Kas-0.
Natural varianti114 – 1141Y → H in strain: cv. Bu-0.
Natural varianti120 – 1201E → G in strain: cv. Bretagny.
Natural varianti122 – 1221M → K in strain: cv. Kas-0.
Natural varianti141 – 1411I → T in strain: cv. Bu-0.
Natural varianti161 – 1611K → E in strain: cv. Kent.
Natural varianti176 – 1761E → K in strain: cv. Kent and cv. Wassilewskija.
Natural varianti182 – 1821R → K in strain: cv. Bu-0, cv. Landsberg erecta, cv. Li-8 and cv. NL2.
Natural varianti184 – 1841K → R in strain: cv. NL2.
Natural varianti186 – 1861T → I in strain: cv. Wassilewskija.
Natural varianti195 – 1951V → I in strain: cv. Co-1.
Natural varianti196 – 1961D → N in strain: cv. Kent.
Natural varianti205 – 2051Q → QL in strain: cv. Bu-2.
Natural varianti209 – 2091L → F in strain: cv. Li-3.
Natural varianti228 – 2281E → G in strain: cv. Bla-1, cv. Bretagny, cv. Bs-0, cv. Bu-0, cv. Bu-2, cv. Co-1, cv. Kas-0, cv. Kent, cv. Landsberg erecta, cv. Li-3, cv. Li-8, cv. Nd-0, cv. NL2 and cv. Wassilewskija.
Natural varianti238 – 2381N → D in strain: cv. Bu-2.
Natural varianti249 – 2491Y → C in strain: cv. NL2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36925 Genomic DNA. Translation: AAA64789.1. Sequence problems.
AC079829 Genomic DNA. Translation: AAG50679.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30674.1.
AB493477 mRNA. Translation: BAH30315.1.
AF061401 Genomic DNA. Translation: AAC67505.1. Sequence problems.
AF061402 Genomic DNA. Translation: AAC67506.1. Sequence problems.
AF061403 Genomic DNA. Translation: AAC67507.1. Sequence problems.
AF061404 Genomic DNA. Translation: AAC67508.1. Sequence problems.
AF061405 Genomic DNA. Translation: AAC67509.1. Sequence problems.
AF061406 Genomic DNA. Translation: AAC67510.1. Sequence problems.
AF061407 Genomic DNA. Translation: AAC67511.1. Sequence problems.
AF061408 Genomic DNA. Translation: AAC67512.1. Sequence problems.
AF061409 Genomic DNA. Translation: AAC67513.1. Sequence problems.
AF061410 Genomic DNA. Translation: AAC67514.1. Sequence problems.
AF061411 Genomic DNA. Translation: AAC67515.1. Sequence problems.
AF061412 Genomic DNA. Translation: AAC67516.1. Sequence problems.
AF061413 Genomic DNA. Translation: AAC67517.1. Sequence problems.
AF061414 Genomic DNA. Translation: AAC67518.1. Sequence problems.
AF061415 Genomic DNA. Translation: AAC67519.1. Sequence problems.
PIRiE86389.
RefSeqiNP_564243.1. NM_102395.2.
UniGeneiAt.41269.

Genome annotation databases

EnsemblPlantsiAT1G26310.1; AT1G26310.1; AT1G26310.
GeneIDi839172.
GrameneiAT1G26310.1; AT1G26310.1; AT1G26310.
KEGGiath:AT1G26310.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36925 Genomic DNA. Translation: AAA64789.1. Sequence problems.
AC079829 Genomic DNA. Translation: AAG50679.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30674.1.
AB493477 mRNA. Translation: BAH30315.1.
AF061401 Genomic DNA. Translation: AAC67505.1. Sequence problems.
AF061402 Genomic DNA. Translation: AAC67506.1. Sequence problems.
AF061403 Genomic DNA. Translation: AAC67507.1. Sequence problems.
AF061404 Genomic DNA. Translation: AAC67508.1. Sequence problems.
AF061405 Genomic DNA. Translation: AAC67509.1. Sequence problems.
AF061406 Genomic DNA. Translation: AAC67510.1. Sequence problems.
AF061407 Genomic DNA. Translation: AAC67511.1. Sequence problems.
AF061408 Genomic DNA. Translation: AAC67512.1. Sequence problems.
AF061409 Genomic DNA. Translation: AAC67513.1. Sequence problems.
AF061410 Genomic DNA. Translation: AAC67514.1. Sequence problems.
AF061411 Genomic DNA. Translation: AAC67515.1. Sequence problems.
AF061412 Genomic DNA. Translation: AAC67516.1. Sequence problems.
AF061413 Genomic DNA. Translation: AAC67517.1. Sequence problems.
AF061414 Genomic DNA. Translation: AAC67518.1. Sequence problems.
AF061415 Genomic DNA. Translation: AAC67519.1. Sequence problems.
PIRiE86389.
RefSeqiNP_564243.1. NM_102395.2.
UniGeneiAt.41269.

3D structure databases

ProteinModelPortaliQ39081.
SMRiQ39081. Positions 2-73, 96-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24408. 5 interactions.
DIPiDIP-33764N.
IntActiQ39081. 5 interactions.
STRINGi3702.AT1G26310.1.

Proteomic databases

PaxDbiQ39081.
PRIDEiQ39081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G26310.1; AT1G26310.1; AT1G26310.
GeneIDi839172.
GrameneiAT1G26310.1; AT1G26310.1; AT1G26310.
KEGGiath:AT1G26310.

Organism-specific databases

TAIRiAT1G26310.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiQ39081.
KOiK09264.
OMAiLIAPDSH.
PhylomeDBiQ39081.

Miscellaneous databases

PROiQ39081.

Gene expression databases

GenevisibleiQ39081. AT.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular basis of the cauliflower phenotype in Arabidopsis."
    Kempin S.A., Savidge B., Yanofsky M.F.
    Science 267:522-525(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-27; CYS-39; GLU-131; 151-GLU--ALA-255 AND 176-GLU--THR-186.
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "ORF cloning and analysis of Arabidopsis transcription factor genes."
    Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y., Takagi M.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Molecular population genetics of the Arabidopsis CAULIFLOWER regulatory gene: nonneutral evolution and naturally occurring variation in floral homeotic function."
    Purugganan M.D., Suddith J.I.
    Proc. Natl. Acad. Sci. U.S.A. 95:8130-8134(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 64-255, VARIANTS.
    Strain: cv. Bla-1, cv. Bretagny, cv. Bs-0, cv. Bu-0, cv. Bu-2, cv. Ch-0, cv. Co-1, cv. Columbia, cv. Firenzi, cv. Kas-0, cv. Kent, cv. Landsberg erecta, cv. Li-3, cv. Li-8, cv. Nd-0, cv. NL2 and cv. Wassilewskija.
  6. "Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes."
    Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R.
    Development 119:721-743(1993)
    Cited for: FUNCTION.
  7. "Redundant regulation of meristem identity and plant architecture by FRUITFULL, APETALA1 and CAULIFLOWER."
    Ferrandiz C., Gu Q., Martienssen R., Yanofsky M.F.
    Development 127:725-734(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCAL_ARATH
AccessioniPrimary (citable) accession number: Q39081
Secondary accession number(s): C0SUX4
, Q9C675, Q9S779, Q9S780, Q9SBG8, Q9SBG9, Q9SBH0, Q9SBH1, Q9SBH2, Q9SBH3, Q9SBH4, Q9SBH5, Q9SBH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: September 22, 2009
Last modified: February 17, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in the CAL gene result in a characteristic proliferation of inflorescence meristems in place of floral meristems. The fragment expressed in cal-5 mutant is homolog to the CAL proteins present in Brassica oleracea var. botrytis and may thus explain the 'cauliflower'-shaped floral meristem (PubMed:7824951).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.