Reviewed, UniProtKB/Swiss-Prot Q39080 (DAD1_ARATH)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
      Short name=Oligosaccharyl transferase subunit DAD1
    EC=2.4.1.119
Alternative name(s):
    Defender against cell death 1
      Short name=DAD-1
      Short name=AtDAD1

Gene names

Name:	DAD1
Ordered Locus Names:	At1g32210
ORF Names:	F3C3.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity. Possesses cell death-inhibiting activity By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the DAD/OST2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anti-apoptosis Ref.1 Inferred from genetic interaction. Source: TAIR apoptosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 115	115	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1		PRO_0000124019
Regions
	Transmembrane	32 – 52	21	Potential
	Transmembrane	56 – 76	21	Potential
Experimental info
	Sequence conflict	102	1	C → Y in CAA64837. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q39080-1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: B944C9EA03AF0705	FASTA	115	12,695
	10 20 30 40 50 60 MVKSTSKDAQ DLFRSLRSAY SATPTNLKII DLYVVFAVFT ALIQVVYMAL VGSFPFNSFL 70 80 90 100 110 SGVLSCIGTA VLAVCLRIQV NKENKEFKDL APERAFADFV LCNLVLHLVI INFLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant." Gallois P., Makishima T., Hecht V., Depres B., Laudii M., Nishimoto T., Cooke R. Plant J. 11:1325-1331(1997) [PubMed: 9225471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Differential regulation of two types of the defender against apoptotic cell death 1 (dad1) genes in senescing cotyledons and petals of Arabidopsis thaliana." Sugiura T., Naito K., Asahi T., Suzuki H. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X95585 mRNA. Translation: CAA64837.1. Y17608 Genomic DNA. Translation: CAC80054.1. AC084165 Genomic DNA. Translation: AAG23438.1. BT024553 mRNA. Translation: ABD38892.1.
IPI	IPI00520612.
PIR	F86446. S71269.
RefSeq	NP_174500.1.
UniGene	At.32
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q39080.
Genome annotation databases
GeneID	840113.
GenomeReviews	Gene locus AT1G32210 in contig CT485782_GR.
KEGG	ath:AT1G32210.
Organism-specific databases
TAIR	At1g32210.
Phylogenomic databases
OMA	YCCLVGT.
Enzyme and pathway databases
BRENDA	2.4.1.119. 302.
Gene expression databases
ArrayExpress	Q39080.
Genevestigator	Q39080.
GermOnline	AT1G32210. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003038. DAD. [Graphical view]
PANTHER	PTHR10705. DAD. 1 hit.
Pfam	PF02109. DAD. 1 hit. [Graphical view]
PIRSF	PIRSF005588. DAD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAD1_ARATH

Accession

Primary (citable) accession number: Q39080
Secondary accession number(s): Q53WQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)