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Protein

Mediator of RNA polymerase II transcription subunit 37f

Gene

MED37F

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation.1 Publication
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to cadmium ion Source: TAIR
  • response to cytokinin Source: TAIR
  • response to endoplasmic reticulum stress Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 37f
Alternative name(s):
Heat shock 70 kDa protein 12
Heat shock protein 70-12
Short name:
AtHsp70-12
Luminal-binding protein 2
Short name:
AtBP2
Short name:
BiP2
Gene namesi
Name:MED37F
Synonyms:BIP, BIP2, HSP70-11, MED37_6
Ordered Locus Names:At5g42020
ORF Names:MJC20.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42020.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation
  • Nucleus Curated

GO - Cellular componenti

  • cell wall Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • mediator complex Source: UniProtKB
  • membrane Source: TAIR
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Disruption phenotypei

Bip1 and bip2 double mutation affects the fusion of polar nuclei during female gametophyte development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001358924 – 668Mediator of RNA polymerase II transcription subunit 37fAdd BLAST645

Proteomic databases

PaxDbiQ39043.
PRIDEiQ39043.

2D gel databases

SWISS-2DPAGEQ39043.

PTM databases

iPTMnetiQ39043.
SwissPalmiQ39043.

Expressioni

Developmental stagei

Down-regulated during seed maturation. Up-regulated during germination.1 Publication

Inductioni

By heat shock, by sugar. Up-regulated by light, DTT and tunicamycin treatment.4 Publications

Gene expression databases

ExpressionAtlasiQ39043. baseline and differential.
GenevisibleiQ39043. AT.

Interactioni

Subunit structurei

Component of the Mediator complex. Interacts with BAG7.3 Publications

Protein-protein interaction databases

BioGridi19457. 10 interactors.
IntActiQ39043. 3 interactors.
MINTiMINT-7950442.
STRINGi3702.AT5G42020.1.

Structurei

3D structure databases

ProteinModelPortaliQ39043.
SMRiQ39043.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi665 – 668Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0100. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ39043.
KOiK09490.
OMAiKPYVQVK.
OrthoDBiEOG093604JO.
PhylomeDBiQ39043.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q39043-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARSFGANST VVLAIIFFGC LFAFSTAKEE ATKLGSVIGI DLGTTYSCVG
60 70 80 90 100
VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD
110 120 130 140 150
VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE
160 170 180 190 200
ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN
210 220 230 240 250
VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV
260 270 280 290 300
LSTNGDTHLG GEDFDHRIME YFIKLIKKKH QKDISKDNKA LGKLRRECER
310 320 330 340 350
AKRALSSQHQ VRVEIESLFD GVDLSEPLTR ARFEELNNDL FRKTMGPVKK
360 370 380 390 400
AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV
410 420 430 440 450
AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV
460 470 480 490 500
IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLTGVPPAPR
510 520 530 540 550
GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV
560 570 580 590 600
KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVSDKDKL ADKLEGDEKE
610 620 630 640 650
KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP
660
GAGGESSTEE EDESHDEL
Length:668
Mass (Da):73,561
Last modified:December 1, 2000 - v2
Checksum:i584DB253E91E68CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163A → T in BAA13948 (Ref. 2) Curated1
Sequence conflicti485R → S in BAA12348 (PubMed:8888624).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84414 mRNA. Translation: BAA12348.1.
D89342 Genomic DNA. Translation: BAA13948.1.
AB017067 Genomic DNA. Translation: BAB08435.1.
CP002688 Genomic DNA. Translation: AED94755.1.
BT002392 mRNA. Translation: AAO00752.1.
BT008406 mRNA. Translation: AAP37765.1.
PIRiS71171.
RefSeqiNP_851119.1. NM_180788.3. [Q39043-1]
UniGeneiAt.23381.
At.33073.
At.49118.
At.49188.

Genome annotation databases

EnsemblPlantsiAT5G42020.1; AT5G42020.1; AT5G42020. [Q39043-1]
GeneIDi834207.
GrameneiAT5G42020.1; AT5G42020.1; AT5G42020.
KEGGiath:AT5G42020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84414 mRNA. Translation: BAA12348.1.
D89342 Genomic DNA. Translation: BAA13948.1.
AB017067 Genomic DNA. Translation: BAB08435.1.
CP002688 Genomic DNA. Translation: AED94755.1.
BT002392 mRNA. Translation: AAO00752.1.
BT008406 mRNA. Translation: AAP37765.1.
PIRiS71171.
RefSeqiNP_851119.1. NM_180788.3. [Q39043-1]
UniGeneiAt.23381.
At.33073.
At.49118.
At.49188.

3D structure databases

ProteinModelPortaliQ39043.
SMRiQ39043.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19457. 10 interactors.
IntActiQ39043. 3 interactors.
MINTiMINT-7950442.
STRINGi3702.AT5G42020.1.

PTM databases

iPTMnetiQ39043.
SwissPalmiQ39043.

2D gel databases

SWISS-2DPAGEQ39043.

Proteomic databases

PaxDbiQ39043.
PRIDEiQ39043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42020.1; AT5G42020.1; AT5G42020. [Q39043-1]
GeneIDi834207.
GrameneiAT5G42020.1; AT5G42020.1; AT5G42020.
KEGGiath:AT5G42020.

Organism-specific databases

TAIRiAT5G42020.

Phylogenomic databases

eggNOGiKOG0100. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ39043.
KOiK09490.
OMAiKPYVQVK.
OrthoDBiEOG093604JO.
PhylomeDBiQ39043.

Miscellaneous databases

PROiQ39043.

Gene expression databases

ExpressionAtlasiQ39043. baseline and differential.
GenevisibleiQ39043. AT.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMD37F_ARATH
AccessioniPrimary (citable) accession number: Q39043
Secondary accession number(s): Q39042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.