Q39043 (BIP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Luminal-binding protein 2
Short name=AtBP2
Short name=BiP2

Gene names

Ordered Locus Names:	At5g42020
ORF Names:	MJC20.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Post-translational modification	Ubiquitinated. Ref.6
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	polar nucleus fusion Inferred from genetic interaction. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR response to endoplasmic reticulum stress Inferred from expression pattern. Source: TAIR
Cellular component	cell wall Inferred from direct assay. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q39043-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 668	645	Luminal-binding protein 2		PRO_0000013589
Regions
	Motif	665 – 668	4	Prevents secretion from ER Potential
Experimental info
	Sequence conflict	163	1	A → T in BAA13948. Ref.2
	Sequence conflict	485	1	R → S in BAA12348. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 584DB253E91E68CA	FASTA	668	73,561
	10 20 30 40 50 60 MARSFGANST VVLAIIFFGC LFAFSTAKEE ATKLGSVIGI DLGTTYSCVG VYKNGHVEII 70 80 90 100 110 120 ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD VKRLIGRKFE DKEVQKDRKL 130 140 150 160 170 180 VPYQIVNKDG KPYIQVKIKD GETKVFSPEE ISAMILTKMK ETAEAYLGKK IKDAVVTVPA 190 200 210 220 230 240 YFNDAQRQAT KDAGVIAGLN VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV 250 260 270 280 290 300 LTIDNGVFEV LSTNGDTHLG GEDFDHRIME YFIKLIKKKH QKDISKDNKA LGKLRRECER 310 320 330 340 350 360 AKRALSSQHQ VRVEIESLFD GVDLSEPLTR ARFEELNNDL FRKTMGPVKK AMDDAGLQKS 370 380 390 400 410 420 QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV AYGAAVQGGI LSGEGGDETK 430 440 450 460 470 480 DILLLDVAPL TLGIETVGGV MTKLIPRNTV IPTKKSQVFT TYQDQQTTVS IQVFEGERSL 490 500 510 520 530 540 TKDCRLLGKF DLTGVPPAPR GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR 550 560 570 580 590 600 LSQEEIDRMV KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVSDKDKL ADKLEGDEKE 610 620 630 640 650 660 KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP GAGGESSTEE EDESHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and responses to stress of a gene that encodes a luminal binding protein in Arabidopsis thaliana." Koizumi N. Plant Cell Physiol. 37:862-865(1996) [PubMed: 8888624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[2]	"Isolation of two genes encoding luminal binding protein from Arabidopsis thaliana." Koizumi N., Sano H. Plant Gene Register PGR97-028 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed: 10470850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed: 19292762] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D84414 mRNA. Translation: BAA12348.1. D89342 Genomic DNA. Translation: BAA13948.1. AB017067 Genomic DNA. Translation: BAB08435.1. CP002688 Genomic DNA. Translation: AED94755.1. BT002392 mRNA. Translation: AAO00752.1. BT008406 mRNA. Translation: AAP37765.1.
IPI	IPI00526247.
PIR	S71171.
RefSeq	NP_851119.1. NM_180788.2.
UniGene	At.23381. At.49118. At.49188.
3D structure databases
ProteinModelPortal	Q39043.
SMR	Q39043. Positions 34-642.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39043. 1 interaction.
STRING	Q39043.
2D gel databases
SWISS-2DPAGE	Q39043.
Proteomic databases
PRIDE	Q39043.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G42020.1; AT5G42020.1; AT5G42020.
GeneID	834207.
GenomeReviews	Gene locus AT5G42020 in contig BA000015_GR.
KEGG	ath:AT5G42020.
NMPDR	fig|3702.1.peg.25921.
Organism-specific databases
TAIR	At5g42020.
Phylogenomic databases
eggNOG	KOG0100.
GeneTree	EPGT00070000028267.
HOGENOM	HBG334976.
InParanoid	Q39043.
OMA	NQLTTNP.
PhylomeDB	Q39043.
ProtClustDB	CLSN2713985.
Gene expression databases
Genevestigator	Q39043.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view]
KO	K09490.
PANTHER	PTHR19375. Hsp70. 1 hit.
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIP2_ARATH

Accession

Primary (citable) accession number: Q39043
Secondary accession number(s): Q39042

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 16, 2011
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program