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Q39043

- MD37F_ARATH

UniProt

Q39043 - MD37F_ARATH

Protein

Mediator of RNA polymerase II transcription subunit 37f

Gene

MED37F

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation.1 Publication
    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. polar nucleus fusion Source: TAIR
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. response to cadmium ion Source: TAIR
    4. response to endoplasmic reticulum stress Source: TAIR
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-1925-MONOMER.
    ARA:GQT-1926-MONOMER.
    ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 37f
    Alternative name(s):
    Heat shock 70 kDa protein 12
    Heat shock protein 70-12
    Short name:
    AtHsp70-12
    Luminal-binding protein 2
    Short name:
    AtBP2
    Short name:
    BiP2
    Gene namesi
    Name:MED37F
    Synonyms:BIP, BIP2, HSP70-11, MED37_6
    Ordered Locus Names:At5g42020
    ORF Names:MJC20.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G42020.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation. Nucleus Curated

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. endoplasmic reticulum Source: TAIR
    3. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    4. mediator complex Source: UniProtKB
    5. membrane Source: TAIR
    6. nucleolus Source: TAIR
    7. nucleus Source: TAIR
    8. plasma membrane Source: TAIR
    9. plasmodesma Source: TAIR
    10. vacuolar membrane Source: TAIR
    11. vacuole Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Bip1 and bip2 double mutation affects the fusion of polar nuclei during female gametophyte development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 668645Mediator of RNA polymerase II transcription subunit 37fPRO_0000013589Add
    BLAST

    Post-translational modificationi

    Ubiquitinated.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ39043.
    PRIDEiQ39043.

    2D gel databases

    SWISS-2DPAGEQ39043.

    Expressioni

    Developmental stagei

    Down-regulated during seed maturation. Up-regulated during germination.1 Publication

    Inductioni

    By heat shock, by sugar. Up-regulated by light, DTT and tunicamycin treatment.4 Publications

    Gene expression databases

    GenevestigatoriQ39043.

    Interactioni

    Subunit structurei

    Component of the Mediator complex. Interacts with BAG7.3 Publications

    Protein-protein interaction databases

    BioGridi19457. 7 interactions.
    IntActiQ39043. 3 interactions.
    MINTiMINT-7950442.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39043.
    SMRiQ39043. Positions 34-652.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi665 – 6684Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0443.
    HOGENOMiHOG000228135.
    InParanoidiQ39043.
    KOiK09490.
    OMAiDKRAVQK.
    PhylomeDBiQ39043.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q39043-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MARSFGANST VVLAIIFFGC LFAFSTAKEE ATKLGSVIGI DLGTTYSCVG    50
    VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD 100
    VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE 150
    ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN 200
    VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV 250
    LSTNGDTHLG GEDFDHRIME YFIKLIKKKH QKDISKDNKA LGKLRRECER 300
    AKRALSSQHQ VRVEIESLFD GVDLSEPLTR ARFEELNNDL FRKTMGPVKK 350
    AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV 400
    AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV 450
    IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLTGVPPAPR 500
    GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV 550
    KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVSDKDKL ADKLEGDEKE 600
    KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP 650
    GAGGESSTEE EDESHDEL 668
    Length:668
    Mass (Da):73,561
    Last modified:December 1, 2000 - v2
    Checksum:i584DB253E91E68CA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti163 – 1631A → T in BAA13948. 1 PublicationCurated
    Sequence conflicti485 – 4851R → S in BAA12348. (PubMed:8888624)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84414 mRNA. Translation: BAA12348.1.
    D89342 Genomic DNA. Translation: BAA13948.1.
    AB017067 Genomic DNA. Translation: BAB08435.1.
    CP002688 Genomic DNA. Translation: AED94755.1.
    BT002392 mRNA. Translation: AAO00752.1.
    BT008406 mRNA. Translation: AAP37765.1.
    PIRiS71171.
    RefSeqiNP_851119.1. NM_180788.2. [Q39043-1]
    UniGeneiAt.23381.
    At.33073.
    At.49118.
    At.49188.

    Genome annotation databases

    EnsemblPlantsiAT5G42020.1; AT5G42020.1; AT5G42020. [Q39043-1]
    GeneIDi834207.
    KEGGiath:AT5G42020.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84414 mRNA. Translation: BAA12348.1 .
    D89342 Genomic DNA. Translation: BAA13948.1 .
    AB017067 Genomic DNA. Translation: BAB08435.1 .
    CP002688 Genomic DNA. Translation: AED94755.1 .
    BT002392 mRNA. Translation: AAO00752.1 .
    BT008406 mRNA. Translation: AAP37765.1 .
    PIRi S71171.
    RefSeqi NP_851119.1. NM_180788.2. [Q39043-1 ]
    UniGenei At.23381.
    At.33073.
    At.49118.
    At.49188.

    3D structure databases

    ProteinModelPortali Q39043.
    SMRi Q39043. Positions 34-652.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19457. 7 interactions.
    IntActi Q39043. 3 interactions.
    MINTi MINT-7950442.

    2D gel databases

    SWISS-2DPAGE Q39043.

    Proteomic databases

    PaxDbi Q39043.
    PRIDEi Q39043.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G42020.1 ; AT5G42020.1 ; AT5G42020 . [Q39043-1 ]
    GeneIDi 834207.
    KEGGi ath:AT5G42020.

    Organism-specific databases

    TAIRi AT5G42020.

    Phylogenomic databases

    eggNOGi COG0443.
    HOGENOMi HOG000228135.
    InParanoidi Q39043.
    KOi K09490.
    OMAi DKRAVQK.
    PhylomeDBi Q39043.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1925-MONOMER.
    ARA:GQT-1926-MONOMER.
    Reactomei REACT_108190. ATF6-alpha activates chaperone genes.

    Gene expression databases

    Genevestigatori Q39043.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and responses to stress of a gene that encodes a luminal binding protein in Arabidopsis thaliana."
      Koizumi N.
      Plant Cell Physiol. 37:862-865(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
      Strain: cv. Landsberg erecta.
    2. "Isolation of two genes encoding luminal binding protein from Arabidopsis thaliana."
      Koizumi N., Sano H.
      Plant Gene Register PGR97-028
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
      DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
      Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
      Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
      Sung D.Y., Vierling E., Guy C.L.
      Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNAK GENE SUBFAMILY, INDUCTION, DEVELOPMENTAL STAGE.
    8. "Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana."
      Tajima H., Koizumi N.
      Biochem. Biophys. Res. Commun. 346:926-930(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY SUGAR.
    9. "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit."
      Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.
      Mol. Cell 26:717-729(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, NOMENCLATURE.
    10. "Light enhances the unfolded protein response as measured by BiP2 gene expression and the secretory GFP-2SC marker in Arabidopsis."
      Lu D.P., Christopher D.A.
      Physiol. Plantarum 134:360-368(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY LIGHT; DTT AND TUNICAMYCIN.
    11. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
    12. "BiP-mediated polar nuclei fusion is essential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana."
      Maruyama D., Endo T., Nishikawa S.
      Proc. Natl. Acad. Sci. U.S.A. 107:1684-1689(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    13. "AtBAG7, an Arabidopsis Bcl-2-associated athanogene, resides in the endoplasmic reticulum and is involved in the unfolded protein response."
      Williams B., Kabbage M., Britt R., Dickman M.B.
      Proc. Natl. Acad. Sci. U.S.A. 107:6088-6093(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BAG7.
    14. "The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress."
      Mathur S., Vyas S., Kapoor S., Tyagi A.K.
      Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.

    Entry informationi

    Entry nameiMD37F_ARATH
    AccessioniPrimary (citable) accession number: Q39043
    Secondary accession number(s): Q39042
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3