Q39043 (MD37F_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 37f

Alternative name(s):
Heat shock 70 kDa protein 12
Heat shock protein 70-12
Short name=AtHsp70-12
Luminal-binding protein 2
Short name=AtBP2
Short name=BiP2

Gene names

Name:	MED37F
Synonyms:	BIP, BIP2, HSP70-11, MED37_6
Ordered Locus Names:	At5g42020
ORF Names:	MJC20.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.12 Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation. Ref.12 In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity. Ref.12
Subunit structure	Component of the Mediator complex. Interacts with BAG7. Ref.9 Ref.13 Ref.14
Subcellular location	Endoplasmic reticulum lumen By similarity. Nucleus Probable.
Developmental stage	Down-regulated during seed maturation. Up-regulated during germination. Ref.7
Induction	By heat shock, by sugar. Up-regulated by light, DTT and tunicamycin treatment. Ref.1 Ref.7 Ref.8 Ref.10
Post-translational modification	Ubiquitinated.
Disruption phenotype	bip1 bip2 double mutation affects the fusion of polar nuclei during female gametophyte development. Ref.12
Sequence similarities	Belongs to the heat shock protein 70 (TC 1.A.33) family. DnaK subfamily. [View classification]

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Endoplasmic reticulum Nucleus
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	polar nucleus fusion Inferred from genetic interaction Ref.12. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR response to endoplasmic reticulum stress Inferred from expression pattern PubMed 18574595. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from direct assay PubMed 16287169. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 16618929PubMed 22923678. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell mediator complex Inferred from direct assay Ref.9. Source: UniProtKB nucleolus Inferred from direct assay PubMed 15496452. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q39043-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 668	645	Mediator of RNA polymerase II transcription subunit 37f		PRO_0000013589
Regions
	Motif	665 – 668	4	Prevents secretion from ER Potential
Experimental info
	Sequence conflict	163	1	A → T in BAA13948. Ref.2
	Sequence conflict	485	1	R → S in BAA12348. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 584DB253E91E68CA	FASTA	668	73,561
	10 20 30 40 50 60 MARSFGANST VVLAIIFFGC LFAFSTAKEE ATKLGSVIGI DLGTTYSCVG VYKNGHVEII 70 80 90 100 110 120 ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD VKRLIGRKFE DKEVQKDRKL 130 140 150 160 170 180 VPYQIVNKDG KPYIQVKIKD GETKVFSPEE ISAMILTKMK ETAEAYLGKK IKDAVVTVPA 190 200 210 220 230 240 YFNDAQRQAT KDAGVIAGLN VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV 250 260 270 280 290 300 LTIDNGVFEV LSTNGDTHLG GEDFDHRIME YFIKLIKKKH QKDISKDNKA LGKLRRECER 310 320 330 340 350 360 AKRALSSQHQ VRVEIESLFD GVDLSEPLTR ARFEELNNDL FRKTMGPVKK AMDDAGLQKS 370 380 390 400 410 420 QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV AYGAAVQGGI LSGEGGDETK 430 440 450 460 470 480 DILLLDVAPL TLGIETVGGV MTKLIPRNTV IPTKKSQVFT TYQDQQTTVS IQVFEGERSL 490 500 510 520 530 540 TKDCRLLGKF DLTGVPPAPR GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR 550 560 570 580 590 600 LSQEEIDRMV KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVSDKDKL ADKLEGDEKE 610 620 630 640 650 660 KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP GAGGESSTEE EDESHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and responses to stress of a gene that encodes a luminal binding protein in Arabidopsis thaliana." Koizumi N. Plant Cell Physiol. 37:862-865(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: cv. Landsberg erecta.
[2]	"Isolation of two genes encoding luminal binding protein from Arabidopsis thaliana." Koizumi N., Sano H. Plant Gene Register PGR97-028 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana." Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M. Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family." Sung D.Y., Vierling E., Guy C.L. Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DNAK GENE SUBFAMILY, INDUCTION, DEVELOPMENTAL STAGE.
[8]	"Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana." Tajima H., Koizumi N. Biochem. Biophys. Res. Commun. 346:926-930(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY SUGAR.
[9]	"Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit." Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S. Mol. Cell 26:717-729(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, NOMENCLATURE.
[10]	"Light enhances the unfolded protein response as measured by BiP2 gene expression and the secretory GFP-2SC marker in Arabidopsis." Lu D.P., Christopher D.A. Physiol. Plantarum 134:360-368(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY LIGHT; DTT AND TUNICAMYCIN.
[11]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
[12]	"BiP-mediated polar nuclei fusion is essential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana." Maruyama D., Endo T., Nishikawa S. Proc. Natl. Acad. Sci. U.S.A. 107:1684-1689(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[13]	"AtBAG7, an Arabidopsis Bcl-2-associated athanogene, resides in the endoplasmic reticulum and is involved in the unfolded protein response." Williams B., Kabbage M., Britt R., Dickman M.B. Proc. Natl. Acad. Sci. U.S.A. 107:6088-6093(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BAG7.
[14]	"The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress." Mathur S., Vyas S., Kapoor S., Tyagi A.K. Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D84414 mRNA. Translation: BAA12348.1. D89342 Genomic DNA. Translation: BAA13948.1. AB017067 Genomic DNA. Translation: BAB08435.1. CP002688 Genomic DNA. Translation: AED94755.1. BT002392 mRNA. Translation: AAO00752.1. BT008406 mRNA. Translation: AAP37765.1.
IPI	IPI00526247.
PIR	S71171.
RefSeq	NP_851119.1. NM_180788.2.
UniGene	At.23381. At.33073. At.49118. At.49188.
3D structure databases
ProteinModelPortal	Q39043.
SMR	Q39043. Positions 34-652.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39043. 1 interaction.
2D gel databases
SWISS-2DPAGE	Q39043.
Proteomic databases
PaxDb	Q39043.
PRIDE	Q39043.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G42020.1; AT5G42020.1; AT5G42020.
GeneID	834207.
KEGG	ath:AT5G42020.
Organism-specific databases
TAIR	At5g42020.
Phylogenomic databases
eggNOG	COG0443.
HOGENOM	HOG000228135.
InParanoid	Q39043.
KO	K09490.
OMA	DKRAVQK.
PhylomeDB	Q39043.
ProtClustDB	CLSN2713985.
Gene expression databases
Genevestigator	Q39043.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MD37F_ARATH

Accession

Primary (citable) accession number: Q39043
Secondary accession number(s): Q39042

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program