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Q39043

- MD37F_ARATH

UniProt

Q39043 - MD37F_ARATH

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Protein
Mediator of RNA polymerase II transcription subunit 37f
Gene
MED37F, BIP, BIP2, HSP70-11, MED37_6, At5g42020, MJC20.12
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation.1 Publication
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity.1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. polar nucleus fusion Source: TAIR
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. response to cadmium ion Source: TAIR
  4. response to endoplasmic reticulum stress Source: TAIR
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1925-MONOMER.
ARA:GQT-1926-MONOMER.
ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 37f
Alternative name(s):
Heat shock 70 kDa protein 12
Heat shock protein 70-12
Short name:
AtHsp70-12
Luminal-binding protein 2
Short name:
AtBP2
Short name:
BiP2
Gene namesi
Name:MED37F
Synonyms:BIP, BIP2, HSP70-11, MED37_6
Ordered Locus Names:At5g42020
ORF Names:MJC20.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G42020.

Subcellular locationi

Endoplasmic reticulum lumen By similarity. Nucleus Inferred

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. endoplasmic reticulum Source: TAIR
  3. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  4. mediator complex Source: UniProtKB
  5. membrane Source: TAIR
  6. nucleolus Source: TAIR
  7. nucleus Source: TAIR
  8. plasma membrane Source: TAIR
  9. plasmodesma Source: TAIR
  10. vacuolar membrane Source: TAIR
  11. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Disruption phenotypei

Bip1 and bip2 double mutation affects the fusion of polar nuclei during female gametophyte development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 668645Mediator of RNA polymerase II transcription subunit 37f
PRO_0000013589Add
BLAST

Post-translational modificationi

Ubiquitinated.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ39043.
PRIDEiQ39043.

2D gel databases

SWISS-2DPAGEQ39043.

Expressioni

Developmental stagei

Down-regulated during seed maturation. Up-regulated during germination.1 Publication

Inductioni

By heat shock, by sugar. Up-regulated by light, DTT and tunicamycin treatment.4 Publications

Gene expression databases

GenevestigatoriQ39043.

Interactioni

Subunit structurei

Component of the Mediator complex. Interacts with BAG7.3 Publications

Protein-protein interaction databases

BioGridi19457. 7 interactions.
IntActiQ39043. 3 interactions.
MINTiMINT-7950442.

Structurei

3D structure databases

ProteinModelPortaliQ39043.
SMRiQ39043. Positions 34-652.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi665 – 6684Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0443.
HOGENOMiHOG000228135.
InParanoidiQ39043.
KOiK09490.
OMAiDKRAVQK.
PhylomeDBiQ39043.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q39043-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MARSFGANST VVLAIIFFGC LFAFSTAKEE ATKLGSVIGI DLGTTYSCVG    50
VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD 100
VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE 150
ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN 200
VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV 250
LSTNGDTHLG GEDFDHRIME YFIKLIKKKH QKDISKDNKA LGKLRRECER 300
AKRALSSQHQ VRVEIESLFD GVDLSEPLTR ARFEELNNDL FRKTMGPVKK 350
AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV 400
AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV 450
IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLTGVPPAPR 500
GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV 550
KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVSDKDKL ADKLEGDEKE 600
KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP 650
GAGGESSTEE EDESHDEL 668
Length:668
Mass (Da):73,561
Last modified:December 1, 2000 - v2
Checksum:i584DB253E91E68CA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti163 – 1631A → T in BAA13948. 1 Publication
Sequence conflicti485 – 4851R → S in BAA12348. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D84414 mRNA. Translation: BAA12348.1.
D89342 Genomic DNA. Translation: BAA13948.1.
AB017067 Genomic DNA. Translation: BAB08435.1.
CP002688 Genomic DNA. Translation: AED94755.1.
BT002392 mRNA. Translation: AAO00752.1.
BT008406 mRNA. Translation: AAP37765.1.
PIRiS71171.
RefSeqiNP_851119.1. NM_180788.2. [Q39043-1]
UniGeneiAt.23381.
At.33073.
At.49118.
At.49188.

Genome annotation databases

EnsemblPlantsiAT5G42020.1; AT5G42020.1; AT5G42020. [Q39043-1]
GeneIDi834207.
KEGGiath:AT5G42020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D84414 mRNA. Translation: BAA12348.1 .
D89342 Genomic DNA. Translation: BAA13948.1 .
AB017067 Genomic DNA. Translation: BAB08435.1 .
CP002688 Genomic DNA. Translation: AED94755.1 .
BT002392 mRNA. Translation: AAO00752.1 .
BT008406 mRNA. Translation: AAP37765.1 .
PIRi S71171.
RefSeqi NP_851119.1. NM_180788.2. [Q39043-1 ]
UniGenei At.23381.
At.33073.
At.49118.
At.49188.

3D structure databases

ProteinModelPortali Q39043.
SMRi Q39043. Positions 34-652.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 19457. 7 interactions.
IntActi Q39043. 3 interactions.
MINTi MINT-7950442.

2D gel databases

SWISS-2DPAGE Q39043.

Proteomic databases

PaxDbi Q39043.
PRIDEi Q39043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G42020.1 ; AT5G42020.1 ; AT5G42020 . [Q39043-1 ]
GeneIDi 834207.
KEGGi ath:AT5G42020.

Organism-specific databases

TAIRi AT5G42020.

Phylogenomic databases

eggNOGi COG0443.
HOGENOMi HOG000228135.
InParanoidi Q39043.
KOi K09490.
OMAi DKRAVQK.
PhylomeDBi Q39043.

Enzyme and pathway databases

BioCyci ARA:GQT-1925-MONOMER.
ARA:GQT-1926-MONOMER.
Reactomei REACT_108190. ATF6-alpha activates chaperone genes.

Gene expression databases

Genevestigatori Q39043.

Family and domain databases

Gene3Di 1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProi IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view ]
Pfami PF00012. HSP70. 1 hit.
[Graphical view ]
PRINTSi PR00301. HEATSHOCK70.
SUPFAMi SSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and responses to stress of a gene that encodes a luminal binding protein in Arabidopsis thaliana."
    Koizumi N.
    Plant Cell Physiol. 37:862-865(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: cv. Landsberg erecta.
  2. "Isolation of two genes encoding luminal binding protein from Arabidopsis thaliana."
    Koizumi N., Sano H.
    Plant Gene Register PGR97-028
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
    Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
    Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
    Sung D.Y., Vierling E., Guy C.L.
    Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNAK GENE SUBFAMILY, INDUCTION, DEVELOPMENTAL STAGE.
  8. "Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana."
    Tajima H., Koizumi N.
    Biochem. Biophys. Res. Commun. 346:926-930(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY SUGAR.
  9. "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit."
    Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.
    Mol. Cell 26:717-729(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, NOMENCLATURE.
  10. "Light enhances the unfolded protein response as measured by BiP2 gene expression and the secretory GFP-2SC marker in Arabidopsis."
    Lu D.P., Christopher D.A.
    Physiol. Plantarum 134:360-368(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY LIGHT; DTT AND TUNICAMYCIN.
  11. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  12. "BiP-mediated polar nuclei fusion is essential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana."
    Maruyama D., Endo T., Nishikawa S.
    Proc. Natl. Acad. Sci. U.S.A. 107:1684-1689(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  13. "AtBAG7, an Arabidopsis Bcl-2-associated athanogene, resides in the endoplasmic reticulum and is involved in the unfolded protein response."
    Williams B., Kabbage M., Britt R., Dickman M.B.
    Proc. Natl. Acad. Sci. U.S.A. 107:6088-6093(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BAG7.
  14. "The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress."
    Mathur S., Vyas S., Kapoor S., Tyagi A.K.
    Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.

Entry informationi

Entry nameiMD37F_ARATH
AccessioniPrimary (citable) accession number: Q39043
Secondary accession number(s): Q39042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi