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Protein

Acid beta-fructofuranosidase 4, vacuolar

Gene

BFRUCT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Possible role in the continued mobilization of sucrose to sink organs. Regulates root elongation.2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by C/VIF1 and C/VIF2.1 Publication

Pathwayi: sucrose metabolism

This protein is involved in the pathway sucrose metabolism, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway sucrose metabolism and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei135 – 1351PROSITE-ProRule annotation
Binding sitei151 – 1511SubstrateBy similarity
Binding sitei159 – 1591SubstrateBy similarity
Binding sitei313 – 3131SubstrateBy similarity
Binding sitei346 – 3461SubstrateBy similarity

GO - Molecular functioni

  • beta-fructofuranosidase activity Source: TAIR
  • sucrose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • cellular response to gibberellin stimulus Source: UniProtKB
  • primary root development Source: UniProtKB
  • response to bacterium Source: UniProtKB
  • sucrose metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Abscisic acid signaling pathway

Enzyme and pathway databases

BioCyciARA:AT1G12240-MONOMER.
UniPathwayiUPA00238.

Protein family/group databases

CAZyiGH32. Glycoside Hydrolase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Acid beta-fructofuranosidase 4, vacuolar (EC:3.2.1.26)
Short name:
At beta fruct4
Short name:
AtBETAFRUCT4
Alternative name(s):
Acid invertase 4
Short name:
AI 4
Acid sucrose hydrolase 4
Vacuolar invertase 4
Short name:
Inv-V4
Short name:
VAC-INV 4
Short name:
VI 4
Gene namesi
Name:BFRUCT4
Synonyms:BETAFRUCT4
Ordered Locus Names:At1g12240
ORF Names:T28K15.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G12240.

Subcellular locationi

GO - Cellular componenti

  • plant-type cell wall Source: TAIR
  • plant-type vacuole Source: UniProtKB
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Disruption phenotypei

Reduced carbon fixation rates during the day, but increased respiration during the night. Shorter roots.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 6767Sequence analysisAdd
BLAST
Propeptidei68 – 10841By similarityPRO_0000417019Add
BLAST
Chaini109 – 664556Acid beta-fructofuranosidase 4, vacuolarPRO_0000417020Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...)By similarity
Glycosylationi280 – 2801N-linked (GlcNAc...) (complex)Sequence analysis
Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence analysis
Glycosylationi498 – 4981N-linked (GlcNAc...)Sequence analysis
Disulfide bondi510 ↔ 558By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ39041.
PRIDEiQ39041.

PTM databases

iPTMnetiQ39041.

Expressioni

Tissue specificityi

Mostly expressed in stems, roots and flowers, and, to a lower extent, in mature leaves.1 Publication

Developmental stagei

Expressed during germination et seedling growth.1 Publication

Inductioni

Induced by gibberellin (e.g. gibberellic acid GA) that accumulates in seeds after red light treatment. Accumulates upon infection with virulent but not with avirulent P.syringae.2 Publications

Gene expression databases

GenevisibleiQ39041. AT.

Interactioni

Subunit structurei

Present in two forms, a 70 kDa monomer and a heterodimer of the 30 kDa and 38 kDa subunits. The ratio of the levels of the two forms within cells appears to be regulated developmentally (By similarity).By similarity

Protein-protein interaction databases

BioGridi23017. 2 interactions.
IntActiQ39041. 2 interactions.
STRINGi3702.AT1G12240.1.

Structurei

3D structure databases

ProteinModelPortaliQ39041.
SMRiQ39041. Positions 110-653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1354Substrate bindingBy similarity
Regioni194 – 1952Substrate bindingBy similarity
Regioni258 – 2592Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 32 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0228. Eukaryota.
COG1621. LUCA.
HOGENOMiHOG000181427.
InParanoidiQ39041.
KOiK01193.
OMAiLADEGRC.
OrthoDBiEOG093605WU.
PhylomeDBiQ39041.

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProiIPR021792. Beta-fructofuranosidase.
IPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamiPF11837. DUF3357. 1 hit.
PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTiSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF75005. SSF75005. 1 hit.
PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSDALLPI SAREEEPLCP YTRLPMADPN QETHGPRRRR PFKGLLAVSF
60 70 80 90 100
GLLFIAFYVA LIATHDGSRS NDEGIDETET ITSRARLAGV SEKRNDGLWK
110 120 130 140 150
LSGDRNTPAF EWNNSMLSWQ RTAFHFQPEQ NWMNDPNGPL FYKGWYHFFY
160 170 180 190 200
QYNPNAAVWG DIVWGHAVSR DLIHWVHLPI AMVADQWYDS NGVWTGSATF
210 220 230 240 250
LPDGSIVMLY TGSTDKAVQV QNLAYPEDPN DPLLLKWVKF PGNPVLVPPP
260 270 280 290 300
GILPKDFRDP TTAWKTSEGK WRITIGSKLN KTGISLVYDT IDFKTYEKLD
310 320 330 340 350
TLLHRVPNTG MWECVDFYPV SKTAGNGLDT SVNGPDVKHI VKASMDDTRF
360 370 380 390 400
DHYAVGTYFD SNGTWIPDDP TIDVGMTASL RYDYGKFYAS KSFYDQNKGR
410 420 430 440 450
RVLWSWIGES DSEASDVQKG WSSLQGIPRT VVLDTKTGKN LVQWPVEEIK
460 470 480 490 500
SLRLSSKQFD LEVGPGSVVP VDVGSAAQLD IEAEFEINKE SLDKIIGNAS
510 520 530 540 550
VVAEAEEFSC EKSGGSTVRG ALGPFGFSVL ATESLSEQTP VYFYVAKGKD
560 570 580 590 600
SELKTFFCTD TSRSSVANDV VKPIYGSVVP VLKGEKLTMR ILVDHSIVEA
610 620 630 640 650
FGQGGRTCIT SRVYPTTAIY GAAKLFLFNN ALDATVTASF TVWQMNSAFI
660
HPYSDEAVRA LSRT
Length:664
Mass (Da):73,844
Last modified:January 1, 1998 - v2
Checksum:i85948869AEDABD8B
GO

Sequence cautioni

The sequence BAC43067 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97749 mRNA. Translation: CAA66330.1.
Y11559 Genomic DNA. Translation: CAA72321.1.
AC022522 Genomic DNA. Translation: AAG12569.1.
CP002684 Genomic DNA. Translation: AEE28855.1.
AY046009 mRNA. Translation: AAK76683.1.
AY142666 mRNA. Translation: AAN13204.1.
AK118459 mRNA. Translation: BAC43067.1. Different initiation.
PIRiE86257.
S71276.
RefSeqiNP_563901.1. NM_101096.2.
UniGeneiAt.24440.

Genome annotation databases

EnsemblPlantsiAT1G12240.1; AT1G12240.1; AT1G12240.
GeneIDi837777.
GrameneiAT1G12240.1; AT1G12240.1; AT1G12240.
KEGGiath:AT1G12240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97749 mRNA. Translation: CAA66330.1.
Y11559 Genomic DNA. Translation: CAA72321.1.
AC022522 Genomic DNA. Translation: AAG12569.1.
CP002684 Genomic DNA. Translation: AEE28855.1.
AY046009 mRNA. Translation: AAK76683.1.
AY142666 mRNA. Translation: AAN13204.1.
AK118459 mRNA. Translation: BAC43067.1. Different initiation.
PIRiE86257.
S71276.
RefSeqiNP_563901.1. NM_101096.2.
UniGeneiAt.24440.

3D structure databases

ProteinModelPortaliQ39041.
SMRiQ39041. Positions 110-653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23017. 2 interactions.
IntActiQ39041. 2 interactions.
STRINGi3702.AT1G12240.1.

Protein family/group databases

CAZyiGH32. Glycoside Hydrolase Family 32.

PTM databases

iPTMnetiQ39041.

Proteomic databases

PaxDbiQ39041.
PRIDEiQ39041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G12240.1; AT1G12240.1; AT1G12240.
GeneIDi837777.
GrameneiAT1G12240.1; AT1G12240.1; AT1G12240.
KEGGiath:AT1G12240.

Organism-specific databases

TAIRiAT1G12240.

Phylogenomic databases

eggNOGiKOG0228. Eukaryota.
COG1621. LUCA.
HOGENOMiHOG000181427.
InParanoidiQ39041.
KOiK01193.
OMAiLADEGRC.
OrthoDBiEOG093605WU.
PhylomeDBiQ39041.

Enzyme and pathway databases

UniPathwayiUPA00238.
BioCyciARA:AT1G12240-MONOMER.

Miscellaneous databases

PROiQ39041.

Gene expression databases

GenevisibleiQ39041. AT.

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProiIPR021792. Beta-fructofuranosidase.
IPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamiPF11837. DUF3357. 1 hit.
PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTiSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF75005. SSF75005. 1 hit.
PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiINVA4_ARATH
AccessioniPrimary (citable) accession number: Q39041
Secondary accession number(s): Q7DLW9, Q8GX36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.