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Protein

Acid beta-fructofuranosidase 4, vacuolar

Gene

BFRUCT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible role in the continued mobilization of sucrose to sink organs (PubMed:20207708). Regulates root elongation (PubMed:16481625).2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by C/VIF1 and C/VIF2.1 Publication

Pathwayi: sucrose metabolism

This protein is involved in the pathway sucrose metabolism, which is part of Glycan biosynthesis.Curated
View all proteins of this organism that are known to be involved in the pathway sucrose metabolism and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei135PROSITE-ProRule annotation1
Binding sitei151SubstrateBy similarity1
Binding sitei159SubstrateBy similarity1
Binding sitei313SubstrateBy similarity1
Binding sitei346SubstrateBy similarity1

GO - Molecular functioni

  • beta-fructofuranosidase activity Source: TAIR
  • sucrose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • cellular response to gibberellin stimulus Source: UniProtKB
  • primary root development Source: UniProtKB
  • response to bacterium Source: UniProtKB
  • sucrose metabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processAbscisic acid signaling pathway

Enzyme and pathway databases

BioCyciARA:AT1G12240-MONOMER.
UniPathwayiUPA00238.

Protein family/group databases

CAZyiGH32. Glycoside Hydrolase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Acid beta-fructofuranosidase 4, vacuolar1 Publication (EC:3.2.1.26PROSITE-ProRule annotation)
Short name:
At beta fruct41 Publication
Short name:
AtBETAFRUCT41 Publication
Alternative name(s):
Acid invertase 41 Publication
Short name:
AI 41 Publication
Acid sucrose hydrolase 4Curated
Vacuolar invertase 41 Publication
Short name:
Inv-V41 Publication
Short name:
VAC-INV 41 Publication
Short name:
VI 41 Publication
Gene namesi
Name:BFRUCT41 Publication
Synonyms:BETAFRUCT41 Publication
Ordered Locus Names:At1g12240Imported
ORF Names:T28K15.3Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2201966. AT1G12240.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43CytoplasmicCuratedAdd BLAST43
Transmembranei44 – 64Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini65 – 664LumenalCuratedAdd BLAST600

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: UniProtKB-SubCell
  • plant-type cell wall Source: TAIR
  • plant-type vacuole Source: UniProtKB
  • vacuolar lumen Source: UniProtKB-SubCell
  • vacuolar membrane Source: UniProtKB-SubCell
  • vacuole Source: TAIR

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Reduced carbon fixation rates during the day, but increased respiration during the night (PubMed:20207708). Shorter roots (PubMed:16481625).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2Missing : No effect on localization. 1 Publication1
Mutagenesisi3 – 4Missing : Endoplasmic reticulum localization. 1 Publication2
Mutagenesisi3Missing : Partial retention in the endoplasmic reticulum. 1 Publication1
Mutagenesisi4Missing : Partial retention in the endoplasmic reticulum. 1 Publication1
Mutagenesisi5Missing : Endoplasmic reticulum localization. 1 Publication1
Mutagenesisi6Missing : No effect on localization. 1 Publication1
Mutagenesisi7 – 8LL → AA: Reduced vacuolar trafficking. 1 Publication2
Mutagenesisi7 – 8Missing : Endoplasmic reticulum localization. 1 Publication2
Mutagenesisi7Missing : Partial retention in the endoplasmic reticulum. 1 Publication1
Mutagenesisi8Missing : Partial retention in the endoplasmic reticulum. 1 Publication1
Mutagenesisi9 – 10PI → AA: Reduced vacuolar trafficking. 1 Publication2
Mutagenesisi9P → A: Partial retention in the endoplasmic reticulum. 1 Publication1
Mutagenesisi10Missing : Endoplasmic reticulum localization. 1 Publication1
Mutagenesisi11Missing : Endoplasmic reticulum localization. 1 Publication1
Mutagenesisi12Missing : No effect on localization. 1 Publication1
Mutagenesisi14 – 16EEE → AAA: Reduced vacuolar trafficking. 1 Publication3
Mutagenesisi18 – 21LCPY → AAAA: Reduced vacuolar trafficking. 1 Publication4
Mutagenesisi21 – 24YTRL → AAAA: Reduced vacuolar trafficking. 1 Publication4
Mutagenesisi21 – 24Missing : No effect on localization. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00004170191 – 108Removed in mature formBy similarityAdd BLAST108
ChainiPRO_0000417020109 – 664Acid beta-fructofuranosidase 4, vacuolarAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Glycosylationi280N-linked (GlcNAc...) (complex)Sequence analysis1
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1
Glycosylationi498N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi510 ↔ 558By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ39041.
PRIDEiQ39041.

PTM databases

iPTMnetiQ39041.

Expressioni

Tissue specificityi

Mostly expressed in stems, roots and flowers, and, to a lower extent, in mature leaves.1 Publication

Developmental stagei

Expressed during germination et seedling growth.1 Publication

Inductioni

Induced by gibberellin (e.g. gibberellic acid GA) that accumulates in seeds after red light treatment (PubMed:15056893). Accumulates upon infection with virulent but not with avirulent P.syringae (PubMed:16807755). Degraded in a VPEgamma-dependent manner during senescence (PubMed:12773619).3 Publications

Gene expression databases

GenevisibleiQ39041. AT.

Interactioni

Subunit structurei

May be present in two forms, a 70 kDa monomer and a heterodimer of the 30 kDa and 38 kDa subunits. The ratio of the levels of the two forms within cells appears to be regulated developmentally (By similarity).By similarity

Protein-protein interaction databases

BioGridi23017. 2 interactors.
IntActiQ39041. 2 interactors.
STRINGi3702.AT1G12240.1.

Structurei

3D structure databases

ProteinModelPortaliQ39041.
SMRiQ39041.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 135Substrate bindingBy similarity4
Regioni194 – 195Substrate bindingBy similarity2
Regioni258 – 259Substrate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 8Critical for endoplasmic reticulum export1 Publication2
Motifi9 – 10Critical for endoplasmic reticulum export1 Publication2
Motifi14 – 16Critical for trafficking from the trans-Golgi network to the prevacuolar compartment and from the prevacuolar compartment to the central vacuole1 Publication3

Domaini

The LCPYTRL domain (18-24) is critical for trafficking from the trans-Golgi network to the prevacuolar compartment and from the prevacuolar compartment to the central vacuole (PubMed:21899678). The PRRRRP domain (36-41) is involved in sorting to the vacuole (PubMed:23737500). At least two Arg are needed for correct delivery and the presence of two neighboring Pro seems to contribute to the sorting efficiency (PubMed:23737500).2 Publications

Sequence similaritiesi

Belongs to the glycosyl hydrolase 32 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0228. Eukaryota.
COG1621. LUCA.
HOGENOMiHOG000181427.
InParanoidiQ39041.
KOiK01193.
OMAiLADEGRC.
OrthoDBiEOG093707IF.
PhylomeDBiQ39041.

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProiView protein in InterPro
IPR021792. Beta-fructofuranosidase.
IPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
PfamiView protein in Pfam
PF11837. DUF3357. 1 hit.
PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
SMARTiView protein in SMART
SM00640. Glyco_32. 1 hit.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF75005. SSF75005. 1 hit.
PROSITEiView protein in PROSITE
PS00609. GLYCOSYL_HYDROL_F32. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSDALLPI SAREEEPLCP YTRLPMADPN QETHGPRRRR PFKGLLAVSF
60 70 80 90 100
GLLFIAFYVA LIATHDGSRS NDEGIDETET ITSRARLAGV SEKRNDGLWK
110 120 130 140 150
LSGDRNTPAF EWNNSMLSWQ RTAFHFQPEQ NWMNDPNGPL FYKGWYHFFY
160 170 180 190 200
QYNPNAAVWG DIVWGHAVSR DLIHWVHLPI AMVADQWYDS NGVWTGSATF
210 220 230 240 250
LPDGSIVMLY TGSTDKAVQV QNLAYPEDPN DPLLLKWVKF PGNPVLVPPP
260 270 280 290 300
GILPKDFRDP TTAWKTSEGK WRITIGSKLN KTGISLVYDT IDFKTYEKLD
310 320 330 340 350
TLLHRVPNTG MWECVDFYPV SKTAGNGLDT SVNGPDVKHI VKASMDDTRF
360 370 380 390 400
DHYAVGTYFD SNGTWIPDDP TIDVGMTASL RYDYGKFYAS KSFYDQNKGR
410 420 430 440 450
RVLWSWIGES DSEASDVQKG WSSLQGIPRT VVLDTKTGKN LVQWPVEEIK
460 470 480 490 500
SLRLSSKQFD LEVGPGSVVP VDVGSAAQLD IEAEFEINKE SLDKIIGNAS
510 520 530 540 550
VVAEAEEFSC EKSGGSTVRG ALGPFGFSVL ATESLSEQTP VYFYVAKGKD
560 570 580 590 600
SELKTFFCTD TSRSSVANDV VKPIYGSVVP VLKGEKLTMR ILVDHSIVEA
610 620 630 640 650
FGQGGRTCIT SRVYPTTAIY GAAKLFLFNN ALDATVTASF TVWQMNSAFI
660
HPYSDEAVRA LSRT
Length:664
Mass (Da):73,844
Last modified:January 1, 1998 - v2
Checksum:i85948869AEDABD8B
GO

Sequence cautioni

The sequence BAC43067 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97749 mRNA. Translation: CAA66330.1.
Y11559 Genomic DNA. Translation: CAA72321.1.
AC022522 Genomic DNA. Translation: AAG12569.1.
CP002684 Genomic DNA. Translation: AEE28855.1.
AY046009 mRNA. Translation: AAK76683.1.
AY142666 mRNA. Translation: AAN13204.1.
AK118459 mRNA. Translation: BAC43067.1. Different initiation.
PIRiE86257.
S71276.
RefSeqiNP_563901.1. NM_101096.3.
UniGeneiAt.24440.

Genome annotation databases

EnsemblPlantsiAT1G12240.1; AT1G12240.1; AT1G12240.
GeneIDi837777.
GrameneiAT1G12240.1; AT1G12240.1; AT1G12240.
KEGGiath:AT1G12240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97749 mRNA. Translation: CAA66330.1.
Y11559 Genomic DNA. Translation: CAA72321.1.
AC022522 Genomic DNA. Translation: AAG12569.1.
CP002684 Genomic DNA. Translation: AEE28855.1.
AY046009 mRNA. Translation: AAK76683.1.
AY142666 mRNA. Translation: AAN13204.1.
AK118459 mRNA. Translation: BAC43067.1. Different initiation.
PIRiE86257.
S71276.
RefSeqiNP_563901.1. NM_101096.3.
UniGeneiAt.24440.

3D structure databases

ProteinModelPortaliQ39041.
SMRiQ39041.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23017. 2 interactors.
IntActiQ39041. 2 interactors.
STRINGi3702.AT1G12240.1.

Protein family/group databases

CAZyiGH32. Glycoside Hydrolase Family 32.

PTM databases

iPTMnetiQ39041.

Proteomic databases

PaxDbiQ39041.
PRIDEiQ39041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G12240.1; AT1G12240.1; AT1G12240.
GeneIDi837777.
GrameneiAT1G12240.1; AT1G12240.1; AT1G12240.
KEGGiath:AT1G12240.

Organism-specific databases

AraportiAT1G12240.
TAIRilocus:2201966. AT1G12240.

Phylogenomic databases

eggNOGiKOG0228. Eukaryota.
COG1621. LUCA.
HOGENOMiHOG000181427.
InParanoidiQ39041.
KOiK01193.
OMAiLADEGRC.
OrthoDBiEOG093707IF.
PhylomeDBiQ39041.

Enzyme and pathway databases

UniPathwayiUPA00238.
BioCyciARA:AT1G12240-MONOMER.

Miscellaneous databases

PROiQ39041.

Gene expression databases

GenevisibleiQ39041. AT.

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProiView protein in InterPro
IPR021792. Beta-fructofuranosidase.
IPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
PfamiView protein in Pfam
PF11837. DUF3357. 1 hit.
PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
SMARTiView protein in SMART
SM00640. Glyco_32. 1 hit.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF75005. SSF75005. 1 hit.
PROSITEiView protein in PROSITE
PS00609. GLYCOSYL_HYDROL_F32. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiINVA4_ARATH
AccessioniPrimary (citable) accession number: Q39041
Secondary accession number(s): Q7DLW9, Q8GX36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: January 1, 1998
Last modified: February 15, 2017
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.