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Protein

Peroxidase 59

Gene

PER59

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
  • Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei66Transition state stabilizer1
Active sitei70Proton acceptor1
Metal bindingi71Calcium 1PROSITE-ProRule annotation1 Publication1
Metal bindingi74Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1 Publication1
Metal bindingi76Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1 Publication1
Metal bindingi78Calcium 1PROSITE-ProRule annotation1 Publication1
Metal bindingi80Calcium 1PROSITE-ProRule annotation1 Publication1
Binding sitei163Substrate; via carbonyl oxygen1
Metal bindingi193Iron (heme axial ligand)1
Metal bindingi194Calcium 2PROSITE-ProRule annotation1 Publication1
Metal bindingi245Calcium 2PROSITE-ProRule annotation1 Publication1
Metal bindingi248Calcium 2PROSITE-ProRule annotation1 Publication1
Metal bindingi251Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation1 Publication1
Metal bindingi253Calcium 2PROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G19890-MONOMER.

Protein family/group databases

PeroxiBasei225. AtPrx59.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 59 (EC:1.11.1.7)
Short name:
Atperox P59
Alternative name(s):
ATPN
Peroxidase N
Gene namesi
Name:PER59
Synonyms:P59
Ordered Locus Names:At5g19890
ORF Names:F28I16.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19890.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000002372429 – 328Peroxidase 59Add BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29Pyrrolidone carboxylic acidPROSITE-ProRule annotationBy similarity1
Disulfide bondi39 ↔ 116PROSITE-ProRule annotation1 Publication
Disulfide bondi72 ↔ 77PROSITE-ProRule annotation1 Publication
Disulfide bondi122 ↔ 323PROSITE-ProRule annotation1 Publication
Glycosylationi182N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi200 ↔ 232PROSITE-ProRule annotation1 Publication
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ39034.
PRIDEiQ39034.

Expressioni

Tissue specificityi

Slightly expressed in roots.

Gene expression databases

GenevisibleiQ39034. AT.

Interactioni

Protein-protein interaction databases

BioGridi17387. 1 interactor.
MINTiMINT-8066504.
STRINGi3702.AT5G19890.1.

Structurei

Secondary structure

1328
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni32 – 38Combined sources7
Helixi42 – 56Combined sources15
Helixi60 – 72Combined sources13
Turni73 – 75Combined sources3
Beta strandi76 – 79Combined sources4
Helixi80 – 82Combined sources3
Helixi89 – 91Combined sources3
Turni93 – 98Combined sources6
Helixi102 – 115Combined sources14
Turni117 – 119Combined sources3
Helixi122 – 135Combined sources14
Turni136 – 138Combined sources3
Helixi156 – 159Combined sources4
Helixi169 – 177Combined sources9
Turni178 – 180Combined sources3
Helixi183 – 190Combined sources8
Helixi191 – 194Combined sources4
Beta strandi195 – 199Combined sources5
Helixi200 – 202Combined sources3
Helixi204 – 206Combined sources3
Beta strandi210 – 214Combined sources5
Helixi222 – 231Combined sources10
Beta strandi241 – 247Combined sources7
Beta strandi249 – 251Combined sources3
Helixi255 – 261Combined sources7
Helixi268 – 275Combined sources8
Turni277 – 283Combined sources7
Helixi284 – 292Combined sources9
Helixi294 – 308Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QGJX-ray1.90A/B29-328[»]
ProteinModelPortaliQ39034.
SMRiQ39034.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ39034.

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IP2G. Eukaryota.
ENOG410YC4V. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ39034.
KOiK00430.
OMAiFFRDFTC.
OrthoDBiEOG09360GUI.
PhylomeDBiQ39034.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTQTKVMGG HVLLTVFTLC MLCSGVRAQL SPDIYAKSCP NLVQIVRKQV
60 70 80 90 100
AIALKAEIRM AASLIRLHFH DCFVNGCDAS LLLDGADSEK LAIPNINSAR
110 120 130 140 150
GFEVIDTIKA AVENACPGVV SCADILTLAA RDSVVLSGGP GWRVALGRKD
160 170 180 190 200
GLVANQNSAN NLPSPFEPLD AIIAKFVAVN LNITDVVALS GAHTFGQAKC
210 220 230 240 250
AVFSNRLFNF TGLGNPDATL ETSLLSNLQT VCPLGGNSNI TAPLDRSTTD
260 270 280 290 300
TFDNNYFKNL LEGKGLLSSD QILFSSDLAV NTTKKLVEAY SRSQSLFFRD
310 320
FTCAMIRMGN ISNGASGEVR TNCRVINN
Length:328
Mass (Da):35,023
Last modified:December 6, 2002 - v2
Checksum:iDABD3854FB2D3B12
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3T → R in AAM65571 (Ref. 5) Curated1
Sequence conflicti213L → A (Ref. 1) Curated1
Sequence conflicti213L → A (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98453 mRNA. Translation: CAA67092.1.
AF296836 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92762.1.
AY123985 mRNA. Translation: AAM74498.1.
BT000582 mRNA. Translation: AAN18151.1.
AY088025 mRNA. Translation: AAM65571.1.
RefSeqiNP_568385.1. NM_121996.3.
UniGeneiAt.143.

Genome annotation databases

EnsemblPlantsiAT5G19890.1; AT5G19890.1; AT5G19890.
GeneIDi832111.
GrameneiAT5G19890.1; AT5G19890.1; AT5G19890.
KEGGiath:AT5G19890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98453 mRNA. Translation: CAA67092.1.
AF296836 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92762.1.
AY123985 mRNA. Translation: AAM74498.1.
BT000582 mRNA. Translation: AAN18151.1.
AY088025 mRNA. Translation: AAM65571.1.
RefSeqiNP_568385.1. NM_121996.3.
UniGeneiAt.143.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QGJX-ray1.90A/B29-328[»]
ProteinModelPortaliQ39034.
SMRiQ39034.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17387. 1 interactor.
MINTiMINT-8066504.
STRINGi3702.AT5G19890.1.

Protein family/group databases

PeroxiBasei225. AtPrx59.

Proteomic databases

PaxDbiQ39034.
PRIDEiQ39034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19890.1; AT5G19890.1; AT5G19890.
GeneIDi832111.
GrameneiAT5G19890.1; AT5G19890.1; AT5G19890.
KEGGiath:AT5G19890.

Organism-specific databases

TAIRiAT5G19890.

Phylogenomic databases

eggNOGiENOG410IP2G. Eukaryota.
ENOG410YC4V. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ39034.
KOiK00430.
OMAiFFRDFTC.
OrthoDBiEOG09360GUI.
PhylomeDBiQ39034.

Enzyme and pathway databases

BioCyciARA:AT5G19890-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ39034.
PROiQ39034.

Gene expression databases

GenevisibleiQ39034. AT.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER59_ARATH
AccessioniPrimary (citable) accession number: Q39034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.