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Reviewed, UniProtKB/Swiss-Prot Q39034 (PER59_ARATH)

Last modified November 25, 2008. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Peroxidase 59
      Short name=Atperox P59
    EC=1.11.1.7
Alternative name(s):
    Peroxidase N
    ATPN
Gene names
Name: PER59
Synonyms: P59
Ordered Locus Names: At5g19890
ORF Names: F28I16.40
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activity

Donor + H(2)O(2) = oxidized donor + 2 H(2)O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit.

Binds 2 calcium ions per subunit.

Subcellular location

SecretedBy similarity.

Tissue specificity

Slightly expressed in roots.

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Sequence similarities

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 328300Peroxidase 59
PRO_0000023724

Sites

Active site701Proton acceptor
Metal binding711Calcium 1
Metal binding741Calcium 1; via carbonyl oxygen
Metal binding761Calcium 1; via carbonyl oxygen
Metal binding781Calcium 1
Metal binding801Calcium 1
Metal binding1931Iron (heme axial ligand)
Metal binding1941Calcium 2
Metal binding2451Calcium 2
Metal binding2481Calcium 2
Metal binding2511Calcium 2; via carbonyl oxygen
Metal binding2531Calcium 2
Binding site1631Substrate; via carbonyl oxygen
Site661Transition state stabilizer

Amino acid modifications

Modified residue291Pyrrolidone carboxylic acid By similarity
Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation2091N-linked (GlcNAc...) Potential
Glycosylation2391N-linked (GlcNAc...) Potential
Glycosylation2811N-linked (GlcNAc...) Potential
Glycosylation3101N-linked (GlcNAc...) Potential
Disulfide bond39 ↔ 116
Disulfide bond72 ↔ 77
Disulfide bond122 ↔ 323
Disulfide bond200 ↔ 232

Experimental info

Sequence conflict31T → R in AAM65571. Ref.4
Sequence conflict2131L → A Ref.1 Ref.4

Secondary structure

.................................................. 328
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q39034-1 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: DABD3854FB2D3B12

FASTA32835,023
        10         20         30         40         50         60 
MKTQTKVMGG HVLLTVFTLC MLCSGVRAQL SPDIYAKSCP NLVQIVRKQV AIALKAEIRM 

        70         80         90        100        110        120 
AASLIRLHFH DCFVNGCDAS LLLDGADSEK LAIPNINSAR GFEVIDTIKA AVENACPGVV 

       130        140        150        160        170        180 
SCADILTLAA RDSVVLSGGP GWRVALGRKD GLVANQNSAN NLPSPFEPLD AIIAKFVAVN 

       190        200        210        220        230        240 
LNITDVVALS GAHTFGQAKC AVFSNRLFNF TGLGNPDATL ETSLLSNLQT VCPLGGNSNI 

       250        260        270        280        290        300 
TAPLDRSTTD TFDNNYFKNL LEGKGLLSSD QILFSSDLAV NTTKKLVEAY SRSQSLFFRD 

       310        320 
FTCAMIRMGN ISNGASGEVR TNCRVINN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis and expression of two peroxidase encoding mRNAs from Arabidopsis thaliana."
Oestergaard L., Welinder K.G.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Computational analyses and annotations of the Arabidopsis peroxidase gene family."
Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G.
FEBS Lett. 433:98-102(1998) [PubMed: 9738941] [Abstract]
Cited for: CHARACTERIZATION.
Strain: cv. Columbia.
[6]"Arabidopsis thaliana peroxidase N: structure of a novel neutral peroxidase."
Mirza O., Henriksen A., Oestergaard L., Welinder K.G., Gajhede M.
Acta Crystallogr. D 56:372-375(2000) [PubMed: 10713531] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH CALCIUM AND HEME, DISULFIDE BONDS.
Strain: cv. Columbia.
[7]"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
Tognolli M., Penel C., Greppin H., Simon P.
Gene 288:129-138(2002) [PubMed: 12034502] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.

Cross-references

Sequence databases

X98453 mRNA. Translation: CAA67092.1.
AF296836 Genomic DNA. No translation available.
AY123985 mRNA. Translation: AAM74498.1.
BT000582 mRNA. Translation: AAN18151.1.
AY088025 mRNA. Translation: AAM65571.1.
RefSeqNP_568385.1.
UniGeneAt.143

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1QGJX-ray1.90A/B29-328[»]
ModBaseSearch...

Protein family/group databases

PeroxiBase225. AtPrx59.

Genome annotation databases

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