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Protein

Peroxidase 59

Gene

PER59

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
  • Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei66 – 661Transition state stabilizer
Active sitei70 – 701Proton acceptor
Metal bindingi71 – 711Calcium 1PROSITE-ProRule annotation1 Publication
Metal bindingi74 – 741Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1 Publication
Metal bindingi76 – 761Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1 Publication
Metal bindingi78 – 781Calcium 1PROSITE-ProRule annotation1 Publication
Metal bindingi80 – 801Calcium 1PROSITE-ProRule annotation1 Publication
Binding sitei163 – 1631Substrate; via carbonyl oxygen
Metal bindingi193 – 1931Iron (heme axial ligand)
Metal bindingi194 – 1941Calcium 2PROSITE-ProRule annotation1 Publication
Metal bindingi245 – 2451Calcium 2PROSITE-ProRule annotation1 Publication
Metal bindingi248 – 2481Calcium 2PROSITE-ProRule annotation1 Publication
Metal bindingi251 – 2511Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation1 Publication
Metal bindingi253 – 2531Calcium 2PROSITE-ProRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G19890-MONOMER.

Protein family/group databases

PeroxiBasei225. AtPrx59.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 59 (EC:1.11.1.7)
Short name:
Atperox P59
Alternative name(s):
ATPN
Peroxidase N
Gene namesi
Name:PER59
Synonyms:P59
Ordered Locus Names:At5g19890
ORF Names:F28I16.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19890.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 328300Peroxidase 59PRO_0000023724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Pyrrolidone carboxylic acidPROSITE-ProRule annotationBy similarity
Disulfide bondi39 ↔ 116PROSITE-ProRule annotation1 Publication
Disulfide bondi72 ↔ 77PROSITE-ProRule annotation1 Publication
Disulfide bondi122 ↔ 323PROSITE-ProRule annotation1 Publication
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence analysis
Disulfide bondi200 ↔ 232PROSITE-ProRule annotation1 Publication
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence analysis
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence analysis
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ39034.
PRIDEiQ39034.

Expressioni

Tissue specificityi

Slightly expressed in roots.

Gene expression databases

GenevisibleiQ39034. AT.

Interactioni

Protein-protein interaction databases

BioGridi17387. 1 interaction.
MINTiMINT-8066504.
STRINGi3702.AT5G19890.1.

Structurei

Secondary structure

1
328
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni32 – 387Combined sources
Helixi42 – 5615Combined sources
Helixi60 – 7213Combined sources
Turni73 – 753Combined sources
Beta strandi76 – 794Combined sources
Helixi80 – 823Combined sources
Helixi89 – 913Combined sources
Turni93 – 986Combined sources
Helixi102 – 11514Combined sources
Turni117 – 1193Combined sources
Helixi122 – 13514Combined sources
Turni136 – 1383Combined sources
Helixi156 – 1594Combined sources
Helixi169 – 1779Combined sources
Turni178 – 1803Combined sources
Helixi183 – 1908Combined sources
Helixi191 – 1944Combined sources
Beta strandi195 – 1995Combined sources
Helixi200 – 2023Combined sources
Helixi204 – 2063Combined sources
Beta strandi210 – 2145Combined sources
Helixi222 – 23110Combined sources
Beta strandi241 – 2477Combined sources
Beta strandi249 – 2513Combined sources
Helixi255 – 2617Combined sources
Helixi268 – 2758Combined sources
Turni277 – 2837Combined sources
Helixi284 – 2929Combined sources
Helixi294 – 30815Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QGJX-ray1.90A/B29-328[»]
ProteinModelPortaliQ39034.
SMRiQ39034. Positions 29-328.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ39034.

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IP2G. Eukaryota.
ENOG410YC4V. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ39034.
KOiK00430.
OMAiFFRDFTC.
OrthoDBiEOG09360GUI.
PhylomeDBiQ39034.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTQTKVMGG HVLLTVFTLC MLCSGVRAQL SPDIYAKSCP NLVQIVRKQV
60 70 80 90 100
AIALKAEIRM AASLIRLHFH DCFVNGCDAS LLLDGADSEK LAIPNINSAR
110 120 130 140 150
GFEVIDTIKA AVENACPGVV SCADILTLAA RDSVVLSGGP GWRVALGRKD
160 170 180 190 200
GLVANQNSAN NLPSPFEPLD AIIAKFVAVN LNITDVVALS GAHTFGQAKC
210 220 230 240 250
AVFSNRLFNF TGLGNPDATL ETSLLSNLQT VCPLGGNSNI TAPLDRSTTD
260 270 280 290 300
TFDNNYFKNL LEGKGLLSSD QILFSSDLAV NTTKKLVEAY SRSQSLFFRD
310 320
FTCAMIRMGN ISNGASGEVR TNCRVINN
Length:328
Mass (Da):35,023
Last modified:December 6, 2002 - v2
Checksum:iDABD3854FB2D3B12
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31T → R in AAM65571 (Ref. 5) Curated
Sequence conflicti213 – 2131L → A (Ref. 1) Curated
Sequence conflicti213 – 2131L → A (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98453 mRNA. Translation: CAA67092.1.
AF296836 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92762.1.
AY123985 mRNA. Translation: AAM74498.1.
BT000582 mRNA. Translation: AAN18151.1.
AY088025 mRNA. Translation: AAM65571.1.
RefSeqiNP_568385.1. NM_121996.2.
UniGeneiAt.143.

Genome annotation databases

EnsemblPlantsiAT5G19890.1; AT5G19890.1; AT5G19890.
GeneIDi832111.
GrameneiAT5G19890.1; AT5G19890.1; AT5G19890.
KEGGiath:AT5G19890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98453 mRNA. Translation: CAA67092.1.
AF296836 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92762.1.
AY123985 mRNA. Translation: AAM74498.1.
BT000582 mRNA. Translation: AAN18151.1.
AY088025 mRNA. Translation: AAM65571.1.
RefSeqiNP_568385.1. NM_121996.2.
UniGeneiAt.143.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QGJX-ray1.90A/B29-328[»]
ProteinModelPortaliQ39034.
SMRiQ39034. Positions 29-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17387. 1 interaction.
MINTiMINT-8066504.
STRINGi3702.AT5G19890.1.

Protein family/group databases

PeroxiBasei225. AtPrx59.

Proteomic databases

PaxDbiQ39034.
PRIDEiQ39034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19890.1; AT5G19890.1; AT5G19890.
GeneIDi832111.
GrameneiAT5G19890.1; AT5G19890.1; AT5G19890.
KEGGiath:AT5G19890.

Organism-specific databases

TAIRiAT5G19890.

Phylogenomic databases

eggNOGiENOG410IP2G. Eukaryota.
ENOG410YC4V. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ39034.
KOiK00430.
OMAiFFRDFTC.
OrthoDBiEOG09360GUI.
PhylomeDBiQ39034.

Enzyme and pathway databases

BioCyciARA:AT5G19890-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ39034.
PROiQ39034.

Gene expression databases

GenevisibleiQ39034. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER59_ARATH
AccessioniPrimary (citable) accession number: Q39034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.