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Protein

Phosphoinositide phospholipase C 2

Gene

PLC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. At physiological calcium concentration, the preferred substrate is phosphatidylinositol 4,5-bisphosphate versus phosphatidylinositol.2 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei118PROSITE-ProRule annotation1
Active sitei164PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • embryo sac development Source: TAIR
  • floral organ development Source: TAIR
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • microsporogenesis Source: TAIR
  • positive regulation of auxin biosynthetic process Source: TAIR

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:MONOMER-1621
MetaCyc:MONOMER-1621
BRENDAi3.1.4.11 399
ReactomeiR-ATH-112043 PLC beta mediated events
R-ATH-114604 GPVI-mediated activation cascade
R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol
R-ATH-202433 Generation of second messenger molecules
R-ATH-399997 Acetylcholine regulates insulin secretion
R-ATH-416476 G alpha (q) signalling events
R-ATH-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-ATH-5607764 CLEC7A (Dectin-1) signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC2
Short name:
AtPLC2
Short name:
PI-PLC2
Gene namesi
Name:PLC2
Ordered Locus Names:At3g08510
ORF Names:T8G24.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G08510
TAIRilocus:2103437 AT3G08510

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241271 – 581Phosphoinositide phospholipase C 2Add BLAST581

Post-translational modificationi

Phosphorylation level varies significantly during early response to bacterial elicitor.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39033
PRIDEiQ39033

PTM databases

iPTMnetiQ39033
SwissPalmiQ39033

Expressioni

Tissue specificityi

Expressed in roots, shoots, leaves and flowers.2 Publications

Inductioni

Not induced by environmental stresses such as dehydration, salinity and low temperature.

Gene expression databases

ExpressionAtlasiQ39033 differential
GenevisibleiQ39033 AT

Interactioni

Protein-protein interaction databases

BioGridi5334, 32 interactors
IntActiQ39033, 1 interactor
MINTiQ39033
STRINGi3702.AT3G08510.1

Structurei

3D structure databases

ProteinModelPortaliQ39033
SMRiQ39033
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 102EF-hand-likeAdd BLAST77
Domaini103 – 248PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST146
Domaini317 – 433PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini439 – 546C2PROSITE-ProRule annotationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi291 – 306Asp-richAdd BLAST16

Domaini

Amino acids 23-36 of the EF-hand-like domain are necessary catalysis but not for binding to lipid vesicles.

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
HOGENOMiHOG000244119
InParanoidiQ39033
KOiK05857
OMAiHELMTLD
OrthoDBiEOG0936050V
PhylomeDBiQ39033

Family and domain databases

Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q39033-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKQTYKVCF CFRRRFRYTA SEAPREIKTI FEKYSENGVM TVDHLHRFLI
60 70 80 90 100
DVQKQDKATR EDAQSIINSA SSLLHRNGLH LDAFFKYLFG DNNPPLALHK
110 120 130 140 150
VHHDMDAPIS HYFIFTGHNS YLTGNQLSSD CSEVPIIDAL KKGVRVIELD
160 170 180 190 200
IWPNSNKDDI DVLHGMTLTT PVGLIKCLKA IRAHAFDVSD YPVVVTLEDH
210 220 230 240 250
LTPDLQSKVA EMVTEIFGEI LFTPPVGESL KEFPSPNSLK RRIIISTKPP
260 270 280 290 300
KEYKEGKDVE VVQKGKDLGD EEVWGREVPS FIQRNKSEAK DDLDGNDDDD
310 320 330 340 350
DDDDEDKSKI NAPPQYKHLI AIHAGKPKGG ITECLKVDPD KVRRLSLSEE
360 370 380 390 400
QLEKAAEKYA KQIVRFTQHN LLRIYPKGTR VTSSNYNPLV GWSHGAQMVA
410 420 430 440 450
FNMQGYGRSL WLMQGMFRAN GGCGYIKKPD LLLKSGSDSD IFDPKATLPV
460 470 480 490 500
KTTLRVTVYM GEGWYFDFRH THFDQYSPPD FYTRVGIAGV PGDTVMKKTK
510 520 530 540 550
TLEDNWIPAW DEVFEFPLTV PELALLRLEV HEYDMSEKDD FGGQTCLPVW
560 570 580
ELSEGIRAFP LHSRKGEKYK SVKLLVKVEF V
Length:581
Mass (Da):66,122
Last modified:November 1, 1996 - v1
Checksum:iE337EB95EC16FC0B
GO

Sequence cautioni

The sequence AAM61037 differs from that shown. Reason: Erroneous termination at position 335. Translated as Leu.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50804 mRNA Translation: BAA09432.1
AC074395 Genomic DNA Translation: AAG50827.1
CP002686 Genomic DNA Translation: AEE74640.1
CP002686 Genomic DNA Translation: AEE74641.1
AF360206 mRNA Translation: AAK25916.1
AY040054 mRNA Translation: AAK64112.1
AY084465 mRNA Translation: AAM61037.1 Sequence problems.
AK221660 mRNA Translation: BAD95335.1
PIRiS71170
RefSeqiNP_001030660.1, NM_001035583.1 [Q39033-1]
NP_187464.1, NM_111686.6 [Q39033-1]
UniGeneiAt.20554

Genome annotation databases

EnsemblPlantsiAT3G08510.1; AT3G08510.1; AT3G08510 [Q39033-1]
AT3G08510.2; AT3G08510.2; AT3G08510 [Q39033-1]
GeneIDi819999
GrameneiAT3G08510.1; AT3G08510.1; AT3G08510 [Q39033-1]
AT3G08510.2; AT3G08510.2; AT3G08510 [Q39033-1]
KEGGiath:AT3G08510

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPLCD2_ARATH
AccessioniPrimary (citable) accession number: Q39033
Secondary accession number(s): Q56XL3, Q8LG47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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