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Q39033

- PLCD2_ARATH

UniProt

Q39033 - PLCD2_ARATH

Protein

Phosphoinositide phospholipase C 2

Gene

PLC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. At physiological calcium concentration, the preferred substrate is phosphatidylinositol 4,5-bisphosphate versus phosphatidylinositol.2 Publications

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei118 – 1181PROSITE-ProRule annotation
    Active sitei164 – 1641PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:GQT-1492-MONOMER.
    ARA:GQT-328-MONOMER.
    MetaCyc:MONOMER-1621.
    BRENDAi3.1.4.11. 399.
    ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoinositide phospholipase C 2 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase PLC2
    Short name:
    AtPLC2
    Short name:
    PI-PLC2
    Gene namesi
    Name:PLC2
    Ordered Locus Names:At3g08510
    ORF Names:T8G24.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G08510.

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 581581Phosphoinositide phospholipase C 2PRO_0000324127Add
    BLAST

    Post-translational modificationi

    Phosphorylation level varies significantly during early response to bacterial elicitor.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ39033.
    PRIDEiQ39033.

    Expressioni

    Tissue specificityi

    Expressed in roots, shoots, leaves and flowers.2 Publications

    Inductioni

    Not induced by environmental stresses such as dehydration, salinity and low temperature.

    Gene expression databases

    ArrayExpressiQ39033.
    GenevestigatoriQ39033.

    Interactioni

    Protein-protein interaction databases

    IntActiQ39033. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39033.
    SMRiQ39033. Positions 26-579.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 10277EF-hand-likeAdd
    BLAST
    Domaini103 – 248146PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini317 – 433117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini439 – 546108C2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi291 – 30616Asp-richAdd
    BLAST

    Domaini

    Amino acids 23-36 of the EF-hand-like domain are necessary catalysis but not for binding to lipid vesicles.

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand-like domain.Curated
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000244119.
    InParanoidiQ39033.
    KOiK05857.
    OMAiDRCFSII.
    PhylomeDBiQ39033.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q39033-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSKQTYKVCF CFRRRFRYTA SEAPREIKTI FEKYSENGVM TVDHLHRFLI    50
    DVQKQDKATR EDAQSIINSA SSLLHRNGLH LDAFFKYLFG DNNPPLALHK 100
    VHHDMDAPIS HYFIFTGHNS YLTGNQLSSD CSEVPIIDAL KKGVRVIELD 150
    IWPNSNKDDI DVLHGMTLTT PVGLIKCLKA IRAHAFDVSD YPVVVTLEDH 200
    LTPDLQSKVA EMVTEIFGEI LFTPPVGESL KEFPSPNSLK RRIIISTKPP 250
    KEYKEGKDVE VVQKGKDLGD EEVWGREVPS FIQRNKSEAK DDLDGNDDDD 300
    DDDDEDKSKI NAPPQYKHLI AIHAGKPKGG ITECLKVDPD KVRRLSLSEE 350
    QLEKAAEKYA KQIVRFTQHN LLRIYPKGTR VTSSNYNPLV GWSHGAQMVA 400
    FNMQGYGRSL WLMQGMFRAN GGCGYIKKPD LLLKSGSDSD IFDPKATLPV 450
    KTTLRVTVYM GEGWYFDFRH THFDQYSPPD FYTRVGIAGV PGDTVMKKTK 500
    TLEDNWIPAW DEVFEFPLTV PELALLRLEV HEYDMSEKDD FGGQTCLPVW 550
    ELSEGIRAFP LHSRKGEKYK SVKLLVKVEF V 581
    Length:581
    Mass (Da):66,122
    Last modified:November 1, 1996 - v1
    Checksum:iE337EB95EC16FC0B
    GO

    Sequence cautioni

    The sequence AAM61037.1 differs from that shown. Reason: Erroneous termination at position 335. Translated as Leu.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50804 mRNA. Translation: BAA09432.1.
    AC074395 Genomic DNA. Translation: AAG50827.1.
    CP002686 Genomic DNA. Translation: AEE74640.1.
    CP002686 Genomic DNA. Translation: AEE74641.1.
    AF360206 mRNA. Translation: AAK25916.1.
    AY040054 mRNA. Translation: AAK64112.1.
    AY084465 mRNA. Translation: AAM61037.1. Sequence problems.
    AK221660 mRNA. Translation: BAD95335.1.
    PIRiS71170.
    RefSeqiNP_001030660.1. NM_001035583.1. [Q39033-1]
    NP_187464.1. NM_111686.5. [Q39033-1]
    UniGeneiAt.20554.

    Genome annotation databases

    EnsemblPlantsiAT3G08510.1; AT3G08510.1; AT3G08510. [Q39033-1]
    AT3G08510.2; AT3G08510.2; AT3G08510. [Q39033-1]
    GeneIDi819999.
    KEGGiath:AT3G08510.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50804 mRNA. Translation: BAA09432.1 .
    AC074395 Genomic DNA. Translation: AAG50827.1 .
    CP002686 Genomic DNA. Translation: AEE74640.1 .
    CP002686 Genomic DNA. Translation: AEE74641.1 .
    AF360206 mRNA. Translation: AAK25916.1 .
    AY040054 mRNA. Translation: AAK64112.1 .
    AY084465 mRNA. Translation: AAM61037.1 . Sequence problems.
    AK221660 mRNA. Translation: BAD95335.1 .
    PIRi S71170.
    RefSeqi NP_001030660.1. NM_001035583.1. [Q39033-1 ]
    NP_187464.1. NM_111686.5. [Q39033-1 ]
    UniGenei At.20554.

    3D structure databases

    ProteinModelPortali Q39033.
    SMRi Q39033. Positions 26-579.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q39033. 1 interaction.

    Proteomic databases

    PaxDbi Q39033.
    PRIDEi Q39033.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G08510.1 ; AT3G08510.1 ; AT3G08510 . [Q39033-1 ]
    AT3G08510.2 ; AT3G08510.2 ; AT3G08510 . [Q39033-1 ]
    GeneIDi 819999.
    KEGGi ath:AT3G08510.

    Organism-specific databases

    TAIRi AT3G08510.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000244119.
    InParanoidi Q39033.
    KOi K05857.
    OMAi DRCFSII.
    PhylomeDBi Q39033.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1492-MONOMER.
    ARA:GQT-328-MONOMER.
    MetaCyc:MONOMER-1621.
    BRENDAi 3.1.4.11. 399.
    Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Gene expression databases

    ArrayExpressi Q39033.
    Genevestigatori Q39033.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana."
      Hirayama T., Mitsukawa N., Shibata D., Shinozaki K.
      Plant Mol. Biol. 34:175-180(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, LACK OF INDUCTION.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 309-581.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-581.
      Strain: cv. Columbia.
    7. "N-terminal EF-hand-like domain is required for phosphoinositide-specific phospholipase C activity in Arabidopsis thaliana."
      Otterhag L., Sommarin M., Pical C.
      FEBS Lett. 497:165-170(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    9. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. La-0.
    10. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
      Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
      New Phytol. 162:643-654(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: FUNCTION, LACK OF INDUCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLCD2_ARATH
    AccessioniPrimary (citable) accession number: Q39033
    Secondary accession number(s): Q56XL3, Q8LG47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3