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Protein

Phosphoinositide phospholipase C 2

Gene

PLC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. At physiological calcium concentration, the preferred substrate is phosphatidylinositol 4,5-bisphosphate versus phosphatidylinositol.2 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei118PROSITE-ProRule annotation1
Active sitei164PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • embryo sac development Source: TAIR
  • floral organ development Source: TAIR
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • microsporogenesis Source: TAIR
  • positive regulation of auxin biosynthetic process Source: TAIR

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1621.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-112043. PLC beta mediated events.
R-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC2
Short name:
AtPLC2
Short name:
PI-PLC2
Gene namesi
Name:PLC2
Ordered Locus Names:At3g08510
ORF Names:T8G24.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G08510.
TAIRilocus:2103437. AT3G08510.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241271 – 581Phosphoinositide phospholipase C 2Add BLAST581

Post-translational modificationi

Phosphorylation level varies significantly during early response to bacterial elicitor.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39033.
PRIDEiQ39033.

PTM databases

iPTMnetiQ39033.
SwissPalmiQ39033.

Expressioni

Tissue specificityi

Expressed in roots, shoots, leaves and flowers.2 Publications

Inductioni

Not induced by environmental stresses such as dehydration, salinity and low temperature.

Gene expression databases

ExpressionAtlasiQ39033. baseline and differential.
GenevisibleiQ39033. AT.

Interactioni

Protein-protein interaction databases

BioGridi5334. 32 interactors.
IntActiQ39033. 1 interactor.
STRINGi3702.AT3G08510.1.

Structurei

3D structure databases

ProteinModelPortaliQ39033.
SMRiQ39033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 102EF-hand-likeAdd BLAST77
Domaini103 – 248PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST146
Domaini317 – 433PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini439 – 546C2PROSITE-ProRule annotationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi291 – 306Asp-richAdd BLAST16

Domaini

Amino acids 23-36 of the EF-hand-like domain are necessary catalysis but not for binding to lipid vesicles.

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ39033.
KOiK05857.
OMAiHELMTLD.
OrthoDBiEOG0936050V.
PhylomeDBiQ39033.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR035892. C2_domain_sf.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q39033-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKQTYKVCF CFRRRFRYTA SEAPREIKTI FEKYSENGVM TVDHLHRFLI
60 70 80 90 100
DVQKQDKATR EDAQSIINSA SSLLHRNGLH LDAFFKYLFG DNNPPLALHK
110 120 130 140 150
VHHDMDAPIS HYFIFTGHNS YLTGNQLSSD CSEVPIIDAL KKGVRVIELD
160 170 180 190 200
IWPNSNKDDI DVLHGMTLTT PVGLIKCLKA IRAHAFDVSD YPVVVTLEDH
210 220 230 240 250
LTPDLQSKVA EMVTEIFGEI LFTPPVGESL KEFPSPNSLK RRIIISTKPP
260 270 280 290 300
KEYKEGKDVE VVQKGKDLGD EEVWGREVPS FIQRNKSEAK DDLDGNDDDD
310 320 330 340 350
DDDDEDKSKI NAPPQYKHLI AIHAGKPKGG ITECLKVDPD KVRRLSLSEE
360 370 380 390 400
QLEKAAEKYA KQIVRFTQHN LLRIYPKGTR VTSSNYNPLV GWSHGAQMVA
410 420 430 440 450
FNMQGYGRSL WLMQGMFRAN GGCGYIKKPD LLLKSGSDSD IFDPKATLPV
460 470 480 490 500
KTTLRVTVYM GEGWYFDFRH THFDQYSPPD FYTRVGIAGV PGDTVMKKTK
510 520 530 540 550
TLEDNWIPAW DEVFEFPLTV PELALLRLEV HEYDMSEKDD FGGQTCLPVW
560 570 580
ELSEGIRAFP LHSRKGEKYK SVKLLVKVEF V
Length:581
Mass (Da):66,122
Last modified:November 1, 1996 - v1
Checksum:iE337EB95EC16FC0B
GO

Sequence cautioni

The sequence AAM61037 differs from that shown. Reason: Erroneous termination at position 335. Translated as Leu.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50804 mRNA. Translation: BAA09432.1.
AC074395 Genomic DNA. Translation: AAG50827.1.
CP002686 Genomic DNA. Translation: AEE74640.1.
CP002686 Genomic DNA. Translation: AEE74641.1.
AF360206 mRNA. Translation: AAK25916.1.
AY040054 mRNA. Translation: AAK64112.1.
AY084465 mRNA. Translation: AAM61037.1. Sequence problems.
AK221660 mRNA. Translation: BAD95335.1.
PIRiS71170.
RefSeqiNP_001030660.1. NM_001035583.1. [Q39033-1]
NP_187464.1. NM_111686.6. [Q39033-1]
UniGeneiAt.20554.

Genome annotation databases

EnsemblPlantsiAT3G08510.1; AT3G08510.1; AT3G08510. [Q39033-1]
AT3G08510.2; AT3G08510.2; AT3G08510. [Q39033-1]
GeneIDi819999.
GrameneiAT3G08510.1; AT3G08510.1; AT3G08510.
AT3G08510.2; AT3G08510.2; AT3G08510.
KEGGiath:AT3G08510.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPLCD2_ARATH
AccessioniPrimary (citable) accession number: Q39033
Secondary accession number(s): Q56XL3, Q8LG47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names