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Q39033

- PLCD2_ARATH

UniProt

Q39033 - PLCD2_ARATH

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Protein
Phosphoinositide phospholipase C 2
Gene
PLC2, At3g08510, T8G24.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. At physiological calcium concentration, the preferred substrate is phosphatidylinositol 4,5-bisphosphate versus phosphatidylinositol.2 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei118 – 1181 By similarity
Active sitei164 – 1641 By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:GQT-1492-MONOMER.
ARA:GQT-328-MONOMER.
MetaCyc:MONOMER-1621.
BRENDAi3.1.4.11. 399.
ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC2
Short name:
AtPLC2
Short name:
PI-PLC2
Gene namesi
Name:PLC2
Ordered Locus Names:At3g08510
ORF Names:T8G24.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G08510.

Subcellular locationi

Cell membrane; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Phosphoinositide phospholipase C 2
PRO_0000324127Add
BLAST

Post-translational modificationi

Phosphorylation level varies significantly during early response to bacterial elicitor.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39033.
PRIDEiQ39033.

Expressioni

Tissue specificityi

Expressed in roots, shoots, leaves and flowers.2 Publications

Inductioni

Not induced by environmental stresses such as dehydration, salinity and low temperature.2 Publications

Gene expression databases

ArrayExpressiQ39033.
GenevestigatoriQ39033.

Interactioni

Protein-protein interaction databases

IntActiQ39033. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ39033.
SMRiQ39033. Positions 26-579.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 10277EF-hand-like
Add
BLAST
Domaini103 – 248146PI-PLC X-box
Add
BLAST
Domaini317 – 433117PI-PLC Y-box
Add
BLAST
Domaini439 – 546108C2
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi291 – 30616Asp-rich
Add
BLAST

Domaini

Amino acids 23-36 of the EF-hand-like domain are necessary catalysis but not for binding to lipid vesicles.

Sequence similaritiesi

Contains 1 C2 domain.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ39033.
KOiK05857.
OMAiDRCFSII.
PhylomeDBiQ39033.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q39033-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSKQTYKVCF CFRRRFRYTA SEAPREIKTI FEKYSENGVM TVDHLHRFLI    50
DVQKQDKATR EDAQSIINSA SSLLHRNGLH LDAFFKYLFG DNNPPLALHK 100
VHHDMDAPIS HYFIFTGHNS YLTGNQLSSD CSEVPIIDAL KKGVRVIELD 150
IWPNSNKDDI DVLHGMTLTT PVGLIKCLKA IRAHAFDVSD YPVVVTLEDH 200
LTPDLQSKVA EMVTEIFGEI LFTPPVGESL KEFPSPNSLK RRIIISTKPP 250
KEYKEGKDVE VVQKGKDLGD EEVWGREVPS FIQRNKSEAK DDLDGNDDDD 300
DDDDEDKSKI NAPPQYKHLI AIHAGKPKGG ITECLKVDPD KVRRLSLSEE 350
QLEKAAEKYA KQIVRFTQHN LLRIYPKGTR VTSSNYNPLV GWSHGAQMVA 400
FNMQGYGRSL WLMQGMFRAN GGCGYIKKPD LLLKSGSDSD IFDPKATLPV 450
KTTLRVTVYM GEGWYFDFRH THFDQYSPPD FYTRVGIAGV PGDTVMKKTK 500
TLEDNWIPAW DEVFEFPLTV PELALLRLEV HEYDMSEKDD FGGQTCLPVW 550
ELSEGIRAFP LHSRKGEKYK SVKLLVKVEF V 581
Length:581
Mass (Da):66,122
Last modified:November 1, 1996 - v1
Checksum:iE337EB95EC16FC0B
GO

Sequence cautioni

The sequence AAM61037.1 differs from that shown. Reason: Erroneous termination at position 335. Translated as Leu.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50804 mRNA. Translation: BAA09432.1.
AC074395 Genomic DNA. Translation: AAG50827.1.
CP002686 Genomic DNA. Translation: AEE74640.1.
CP002686 Genomic DNA. Translation: AEE74641.1.
AF360206 mRNA. Translation: AAK25916.1.
AY040054 mRNA. Translation: AAK64112.1.
AY084465 mRNA. Translation: AAM61037.1. Sequence problems.
AK221660 mRNA. Translation: BAD95335.1.
PIRiS71170.
RefSeqiNP_001030660.1. NM_001035583.1. [Q39033-1]
NP_187464.1. NM_111686.5. [Q39033-1]
UniGeneiAt.20554.

Genome annotation databases

EnsemblPlantsiAT3G08510.1; AT3G08510.1; AT3G08510. [Q39033-1]
AT3G08510.2; AT3G08510.2; AT3G08510. [Q39033-1]
GeneIDi819999.
KEGGiath:AT3G08510.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50804 mRNA. Translation: BAA09432.1 .
AC074395 Genomic DNA. Translation: AAG50827.1 .
CP002686 Genomic DNA. Translation: AEE74640.1 .
CP002686 Genomic DNA. Translation: AEE74641.1 .
AF360206 mRNA. Translation: AAK25916.1 .
AY040054 mRNA. Translation: AAK64112.1 .
AY084465 mRNA. Translation: AAM61037.1 . Sequence problems.
AK221660 mRNA. Translation: BAD95335.1 .
PIRi S71170.
RefSeqi NP_001030660.1. NM_001035583.1. [Q39033-1 ]
NP_187464.1. NM_111686.5. [Q39033-1 ]
UniGenei At.20554.

3D structure databases

ProteinModelPortali Q39033.
SMRi Q39033. Positions 26-579.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q39033. 1 interaction.

Proteomic databases

PaxDbi Q39033.
PRIDEi Q39033.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G08510.1 ; AT3G08510.1 ; AT3G08510 . [Q39033-1 ]
AT3G08510.2 ; AT3G08510.2 ; AT3G08510 . [Q39033-1 ]
GeneIDi 819999.
KEGGi ath:AT3G08510.

Organism-specific databases

TAIRi AT3G08510.

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000244119.
InParanoidi Q39033.
KOi K05857.
OMAi DRCFSII.
PhylomeDBi Q39033.

Enzyme and pathway databases

BioCyci ARA:GQT-1492-MONOMER.
ARA:GQT-328-MONOMER.
MetaCyc:MONOMER-1621.
BRENDAi 3.1.4.11. 399.
Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

Gene expression databases

ArrayExpressi Q39033.
Genevestigatori Q39033.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana."
    Hirayama T., Mitsukawa N., Shibata D., Shinozaki K.
    Plant Mol. Biol. 34:175-180(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, LACK OF INDUCTION.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 309-581.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-581.
    Strain: cv. Columbia.
  7. "N-terminal EF-hand-like domain is required for phosphoinositide-specific phospholipase C activity in Arabidopsis thaliana."
    Otterhag L., Sommarin M., Pical C.
    FEBS Lett. 497:165-170(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  10. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
    Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
    New Phytol. 162:643-654(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: FUNCTION, LACK OF INDUCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCD2_ARATH
AccessioniPrimary (citable) accession number: Q39033
Secondary accession number(s): Q56XL3, Q8LG47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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