Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoinositide phospholipase C 1

Gene

PLC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for secondary responses to abscisic acid signals.3 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei120PROSITE-ProRule annotation1
Active sitei166PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1622.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC1
Short name:
AtPLC1
Short name:
AtPLC1S
Short name:
PI-PLC1
Gene namesi
Name:PLC1
Synonyms:ATHATPLC1G
Ordered Locus Names:At5g58670
ORF Names:MZN1.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G58670.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241261 – 561Phosphoinositide phospholipase C 1Add BLAST561

Proteomic databases

PaxDbiQ39032.

PTM databases

iPTMnetiQ39032.
SwissPalmiQ39032.

Expressioni

Tissue specificityi

Expressed in stems, leaves, roots, flowers and siliques. Predominant in the vascular tissues of roots and leaves.2 Publications

Inductioni

By abscisic acid, osmotic stress and environmental stresses such as dehydration, salinity and low temperature.2 Publications

Gene expression databases

GenevisibleiQ39032. AT.

Interactioni

Protein-protein interaction databases

BioGridi21225. 1 interactor.
STRINGi3702.AT5G58670.1.

Structurei

3D structure databases

ProteinModelPortaliQ39032.
SMRiQ39032.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 54EF-handAdd BLAST34
Domaini105 – 249PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST145
Domaini294 – 410PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini417 – 524C2PROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.Curated
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ39032.
KOiK05857.
OMAiKVCFCCV.
OrthoDBiEOG0936050V.
PhylomeDBiQ39032.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKESFKVCFC CVRNFKVKSS EPPEEIKNLF HDYSQDDRMS ADEMLRFVIQ
60 70 80 90 100
VQGETHADIN YVKDIFHRLK HHGVFHPRGI HLEGFYRYLL SDFNSPLPLT
110 120 130 140 150
REVWQDMNQP LSHYFLYTGH NSYLTGNQLN SNSSIEPIVK ALRNGVRVIE
160 170 180 190 200
LDLWPNSSGK EAEVRHGGTL TSREDLQKCL NVVKENAFQV SAYPVVLTLE
210 220 230 240 250
DHLTPILQKK VAKMVSKTFG GSLFQCTDET TECFPSPESL KNKILISTKP
260 270 280 290 300
PKEYLQTQIS KGSTTDESTR AKKISDAEEQ VQEEDEESVA IEYRDLISIH
310 320 330 340 350
AGNRKGGLKN CLNGDPNRVI RLSMSEQWLE TLAKTRGPDL VKFTQRNLLR
360 370 380 390 400
IFPKTTRFDS SNYDPLVGWI HGAQMVAFNM QSHGRYLWMM QGMFKANGGC
410 420 430 440 450
GYVKKPDVLL SNGPEGEIFD PCSQNLPIKT TLKVKIYTGE GWNMDFPLDH
460 470 480 490 500
FDRYSPPDFY AKVGIAGVPL DTASYRTEID KDEWFPIWDK EFEFPLRVPE
510 520 530 540 550
LSLLCITVKD YDSNTQNDFA GQTCFPLSEV RPGIRAVRLH DRAGEVYKHV
560
RLLMRFVLEP R
Length:561
Mass (Da):64,314
Last modified:November 1, 1996 - v1
Checksum:iA05AD123411352E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87R → G in AAC05023 (PubMed:9427559).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38544 mRNA. Translation: BAA07547.1.
U76423 Genomic DNA. Translation: AAC05023.1.
AB020755 Genomic DNA. Translation: BAA97336.1.
CP002688 Genomic DNA. Translation: AED97083.1.
RefSeqiNP_568881.1. NM_125254.2.
UniGeneiAt.10551.

Genome annotation databases

EnsemblPlantsiAT5G58670.1; AT5G58670.1; AT5G58670.
GeneIDi835981.
GrameneiAT5G58670.1; AT5G58670.1; AT5G58670.
KEGGiath:AT5G58670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38544 mRNA. Translation: BAA07547.1.
U76423 Genomic DNA. Translation: AAC05023.1.
AB020755 Genomic DNA. Translation: BAA97336.1.
CP002688 Genomic DNA. Translation: AED97083.1.
RefSeqiNP_568881.1. NM_125254.2.
UniGeneiAt.10551.

3D structure databases

ProteinModelPortaliQ39032.
SMRiQ39032.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21225. 1 interactor.
STRINGi3702.AT5G58670.1.

PTM databases

iPTMnetiQ39032.
SwissPalmiQ39032.

Proteomic databases

PaxDbiQ39032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58670.1; AT5G58670.1; AT5G58670.
GeneIDi835981.
GrameneiAT5G58670.1; AT5G58670.1; AT5G58670.
KEGGiath:AT5G58670.

Organism-specific databases

TAIRiAT5G58670.

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ39032.
KOiK05857.
OMAiKVCFCCV.
OrthoDBiEOG0936050V.
PhylomeDBiQ39032.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1622.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

PROiQ39032.

Gene expression databases

GenevisibleiQ39032. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLCD1_ARATH
AccessioniPrimary (citable) accession number: Q39032
Secondary accession number(s): O49970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.