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Q39032

- PLCD1_ARATH

UniProt

Q39032 - PLCD1_ARATH

Protein

Phosphoinositide phospholipase C 1

Gene

PLC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for secondary responses to abscisic acid signals.3 Publications

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei120 – 1201PROSITE-ProRule annotation
    Active sitei166 – 1661PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. phospholipase C activity Source: TAIR
    4. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. abscisic acid-activated signaling pathway Source: TAIR
    2. intracellular signal transduction Source: InterPro
    3. lipid catabolic process Source: UniProtKB-KW
    4. response to abscisic acid Source: TAIR
    5. response to cold Source: TAIR
    6. response to salt stress Source: TAIR
    7. response to water deprivation Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-1622.
    ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoinositide phospholipase C 1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase PLC1
    Short name:
    AtPLC1
    Short name:
    AtPLC1S
    Short name:
    PI-PLC1
    Gene namesi
    Name:PLC1
    Synonyms:ATHATPLC1G
    Ordered Locus Names:At5g58670
    ORF Names:MZN1.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G58670.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 561561Phosphoinositide phospholipase C 1PRO_0000324126Add
    BLAST

    Proteomic databases

    PaxDbiQ39032.
    PRIDEiQ39032.

    Expressioni

    Tissue specificityi

    Expressed in stems, leaves, roots, flowers and siliques. Predominant in the vascular tissues of roots and leaves.2 Publications

    Inductioni

    By abscisic acid, osmotic stress and environmental stresses such as dehydration, salinity and low temperature.2 Publications

    Gene expression databases

    GenevestigatoriQ39032.

    Interactioni

    Protein-protein interaction databases

    BioGridi21225. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39032.
    SMRiQ39032. Positions 78-559.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 5434EF-handAdd
    BLAST
    Domaini105 – 249145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini294 – 410117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini417 – 524108C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.Curated
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000244119.
    InParanoidiQ39032.
    KOiK05857.
    OMAiTHADINY.
    PhylomeDBiQ39032.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q39032-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKESFKVCFC CVRNFKVKSS EPPEEIKNLF HDYSQDDRMS ADEMLRFVIQ    50
    VQGETHADIN YVKDIFHRLK HHGVFHPRGI HLEGFYRYLL SDFNSPLPLT 100
    REVWQDMNQP LSHYFLYTGH NSYLTGNQLN SNSSIEPIVK ALRNGVRVIE 150
    LDLWPNSSGK EAEVRHGGTL TSREDLQKCL NVVKENAFQV SAYPVVLTLE 200
    DHLTPILQKK VAKMVSKTFG GSLFQCTDET TECFPSPESL KNKILISTKP 250
    PKEYLQTQIS KGSTTDESTR AKKISDAEEQ VQEEDEESVA IEYRDLISIH 300
    AGNRKGGLKN CLNGDPNRVI RLSMSEQWLE TLAKTRGPDL VKFTQRNLLR 350
    IFPKTTRFDS SNYDPLVGWI HGAQMVAFNM QSHGRYLWMM QGMFKANGGC 400
    GYVKKPDVLL SNGPEGEIFD PCSQNLPIKT TLKVKIYTGE GWNMDFPLDH 450
    FDRYSPPDFY AKVGIAGVPL DTASYRTEID KDEWFPIWDK EFEFPLRVPE 500
    LSLLCITVKD YDSNTQNDFA GQTCFPLSEV RPGIRAVRLH DRAGEVYKHV 550
    RLLMRFVLEP R 561
    Length:561
    Mass (Da):64,314
    Last modified:November 1, 1996 - v1
    Checksum:iA05AD123411352E8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti87 – 871R → G in AAC05023. (PubMed:9427559)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38544 mRNA. Translation: BAA07547.1.
    U76423 Genomic DNA. Translation: AAC05023.1.
    AB020755 Genomic DNA. Translation: BAA97336.1.
    CP002688 Genomic DNA. Translation: AED97083.1.
    RefSeqiNP_568881.1. NM_125254.1.
    UniGeneiAt.10551.

    Genome annotation databases

    EnsemblPlantsiAT5G58670.1; AT5G58670.1; AT5G58670.
    GeneIDi835981.
    KEGGiath:AT5G58670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38544 mRNA. Translation: BAA07547.1 .
    U76423 Genomic DNA. Translation: AAC05023.1 .
    AB020755 Genomic DNA. Translation: BAA97336.1 .
    CP002688 Genomic DNA. Translation: AED97083.1 .
    RefSeqi NP_568881.1. NM_125254.1.
    UniGenei At.10551.

    3D structure databases

    ProteinModelPortali Q39032.
    SMRi Q39032. Positions 78-559.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21225. 1 interaction.

    Proteomic databases

    PaxDbi Q39032.
    PRIDEi Q39032.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G58670.1 ; AT5G58670.1 ; AT5G58670 .
    GeneIDi 835981.
    KEGGi ath:AT5G58670.

    Organism-specific databases

    TAIRi AT5G58670.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000244119.
    InParanoidi Q39032.
    KOi K05857.
    OMAi THADINY.
    PhylomeDBi Q39032.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-1622.
    Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Gene expression databases

    Genevestigatori Q39032.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana."
      Hirayama T., Ohto C., Mizoguchi T., Shinozaki K.
      Proc. Natl. Acad. Sci. U.S.A. 92:3903-3907(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Columbia.
    2. "The Arabidopsis thaliana genome has multiple divergent forms of phosphoinositol-specific phospholipase C1."
      Hartweck L.M., Llewellyn D.J., Dennis E.S.
      Gene 202:151-156(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. C24.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Arabidopsis PLC1 is required for secondary responses to abscisic acid signals."
      Sanchez J.-P., Chua N.-H.
      Plant Cell 13:1143-1154(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
      Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
      New Phytol. 162:643-654(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLCD1_ARATH
    AccessioniPrimary (citable) accession number: Q39032
    Secondary accession number(s): O49970
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3