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Q39032 (PLCD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoinositide phospholipase C 1

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase PLC1
Short name=AtPLC1
Short name=AtPLC1S
Short name=PI-PLC1
Gene names
Name:PLC1
Synonyms:ATHATPLC1G
Ordered Locus Names:At5g58670
ORF Names:MZN1.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for secondary responses to abscisic acid signals. Ref.1 Ref.5 Ref.7

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Calcium.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed in stems, leaves, roots, flowers and siliques. Predominant in the vascular tissues of roots and leaves. Ref.1 Ref.7

Induction

By abscisic acid, osmotic stress and environmental stresses such as dehydration, salinity and low temperature. Ref.1 Ref.7

Sequence similarities

Contains 1 C2 domain.

Contains 1 EF-hand domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 561561Phosphoinositide phospholipase C 1
PRO_0000324126

Regions

Domain21 – 5434EF-hand
Domain105 – 249145PI-PLC X-box
Domain294 – 410117PI-PLC Y-box
Domain417 – 524108C2

Sites

Active site1201 By similarity
Active site1661 By similarity

Experimental info

Sequence conflict871R → G in AAC05023. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q39032 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A05AD123411352E8

FASTA56164,314
        10         20         30         40         50         60 
MKESFKVCFC CVRNFKVKSS EPPEEIKNLF HDYSQDDRMS ADEMLRFVIQ VQGETHADIN 

        70         80         90        100        110        120 
YVKDIFHRLK HHGVFHPRGI HLEGFYRYLL SDFNSPLPLT REVWQDMNQP LSHYFLYTGH 

       130        140        150        160        170        180 
NSYLTGNQLN SNSSIEPIVK ALRNGVRVIE LDLWPNSSGK EAEVRHGGTL TSREDLQKCL 

       190        200        210        220        230        240 
NVVKENAFQV SAYPVVLTLE DHLTPILQKK VAKMVSKTFG GSLFQCTDET TECFPSPESL 

       250        260        270        280        290        300 
KNKILISTKP PKEYLQTQIS KGSTTDESTR AKKISDAEEQ VQEEDEESVA IEYRDLISIH 

       310        320        330        340        350        360 
AGNRKGGLKN CLNGDPNRVI RLSMSEQWLE TLAKTRGPDL VKFTQRNLLR IFPKTTRFDS 

       370        380        390        400        410        420 
SNYDPLVGWI HGAQMVAFNM QSHGRYLWMM QGMFKANGGC GYVKKPDVLL SNGPEGEIFD 

       430        440        450        460        470        480 
PCSQNLPIKT TLKVKIYTGE GWNMDFPLDH FDRYSPPDFY AKVGIAGVPL DTASYRTEID 

       490        500        510        520        530        540 
KDEWFPIWDK EFEFPLRVPE LSLLCITVKD YDSNTQNDFA GQTCFPLSEV RPGIRAVRLH 

       550        560 
DRAGEVYKHV RLLMRFVLEP R 

« Hide

References

« Hide 'large scale' references
[1]"A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana."
Hirayama T., Ohto C., Mizoguchi T., Shinozaki K.
Proc. Natl. Acad. Sci. U.S.A. 92:3903-3907(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Columbia.
[2]"The Arabidopsis thaliana genome has multiple divergent forms of phosphoinositol-specific phospholipase C1."
Hartweck L.M., Llewellyn D.J., Dennis E.S.
Gene 202:151-156(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. C24.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Arabidopsis PLC1 is required for secondary responses to abscisic acid signals."
Sanchez J.-P., Chua N.-H.
Plant Cell 13:1143-1154(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
Mueller-Roeber B., Pical C.
Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
New Phytol. 162:643-654(2004) [AGRICOLA] [Europe PMC]
Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D38544 mRNA. Translation: BAA07547.1.
U76423 Genomic DNA. Translation: AAC05023.1.
AB020755 Genomic DNA. Translation: BAA97336.1.
CP002688 Genomic DNA. Translation: AED97083.1.
RefSeqNP_568881.1. NM_125254.1.
UniGeneAt.10551.

3D structure databases

ProteinModelPortalQ39032.
SMRQ39032. Positions 78-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21225. 1 interaction.

Proteomic databases

PaxDbQ39032.
PRIDEQ39032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G58670.1; AT5G58670.1; AT5G58670.
GeneID835981.
KEGGath:AT5G58670.

Organism-specific databases

TAIRAT5G58670.

Phylogenomic databases

eggNOGNOG149692.
HOGENOMHOG000244119.
InParanoidQ39032.
KOK05857.
OMATHADINY.
PhylomeDBQ39032.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-1622.

Gene expression databases

GenevestigatorQ39032.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLCD1_ARATH
AccessionPrimary (citable) accession number: Q39032
Secondary accession number(s): O49970
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names