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Protein

Phosphoinositide phospholipase C 1

Gene

PLC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for secondary responses to abscisic acid signals.3 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei120 – 1201PROSITE-ProRule annotation
Active sitei166 – 1661PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1622.
BRENDAi3.1.4.11. 399.
ReactomeiREACT_278077. Role of phospholipids in phagocytosis.
REACT_278842. Role of second messengers in netrin-1 signaling.
REACT_279237. VEGFR2 mediated cell proliferation.
REACT_284166. Generation of second messenger molecules.
REACT_286162. PLC beta mediated events.
REACT_306790. GPVI-mediated activation cascade.
REACT_328347. DAG and IP3 signaling.
REACT_350143. Synthesis of IP3 and IP4 in the cytosol.
REACT_361272. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC1
Short name:
AtPLC1
Short name:
AtPLC1S
Short name:
PI-PLC1
Gene namesi
Name:PLC1
Synonyms:ATHATPLC1G
Ordered Locus Names:At5g58670
ORF Names:MZN1.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G58670.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Phosphoinositide phospholipase C 1PRO_0000324126Add
BLAST

Proteomic databases

PaxDbiQ39032.
PRIDEiQ39032.

Expressioni

Tissue specificityi

Expressed in stems, leaves, roots, flowers and siliques. Predominant in the vascular tissues of roots and leaves.2 Publications

Inductioni

By abscisic acid, osmotic stress and environmental stresses such as dehydration, salinity and low temperature.2 Publications

Gene expression databases

GenevestigatoriQ39032.

Interactioni

Protein-protein interaction databases

BioGridi21225. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ39032.
SMRiQ39032. Positions 78-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 5434EF-handAdd
BLAST
Domaini105 – 249145PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini294 – 410117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini417 – 524108C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.Curated
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ39032.
KOiK05857.
OMAiTHADINY.
PhylomeDBiQ39032.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKESFKVCFC CVRNFKVKSS EPPEEIKNLF HDYSQDDRMS ADEMLRFVIQ
60 70 80 90 100
VQGETHADIN YVKDIFHRLK HHGVFHPRGI HLEGFYRYLL SDFNSPLPLT
110 120 130 140 150
REVWQDMNQP LSHYFLYTGH NSYLTGNQLN SNSSIEPIVK ALRNGVRVIE
160 170 180 190 200
LDLWPNSSGK EAEVRHGGTL TSREDLQKCL NVVKENAFQV SAYPVVLTLE
210 220 230 240 250
DHLTPILQKK VAKMVSKTFG GSLFQCTDET TECFPSPESL KNKILISTKP
260 270 280 290 300
PKEYLQTQIS KGSTTDESTR AKKISDAEEQ VQEEDEESVA IEYRDLISIH
310 320 330 340 350
AGNRKGGLKN CLNGDPNRVI RLSMSEQWLE TLAKTRGPDL VKFTQRNLLR
360 370 380 390 400
IFPKTTRFDS SNYDPLVGWI HGAQMVAFNM QSHGRYLWMM QGMFKANGGC
410 420 430 440 450
GYVKKPDVLL SNGPEGEIFD PCSQNLPIKT TLKVKIYTGE GWNMDFPLDH
460 470 480 490 500
FDRYSPPDFY AKVGIAGVPL DTASYRTEID KDEWFPIWDK EFEFPLRVPE
510 520 530 540 550
LSLLCITVKD YDSNTQNDFA GQTCFPLSEV RPGIRAVRLH DRAGEVYKHV
560
RLLMRFVLEP R
Length:561
Mass (Da):64,314
Last modified:November 1, 1996 - v1
Checksum:iA05AD123411352E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871R → G in AAC05023 (PubMed:9427559).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38544 mRNA. Translation: BAA07547.1.
U76423 Genomic DNA. Translation: AAC05023.1.
AB020755 Genomic DNA. Translation: BAA97336.1.
CP002688 Genomic DNA. Translation: AED97083.1.
RefSeqiNP_568881.1. NM_125254.1.
UniGeneiAt.10551.

Genome annotation databases

EnsemblPlantsiAT5G58670.1; AT5G58670.1; AT5G58670.
GeneIDi835981.
KEGGiath:AT5G58670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38544 mRNA. Translation: BAA07547.1.
U76423 Genomic DNA. Translation: AAC05023.1.
AB020755 Genomic DNA. Translation: BAA97336.1.
CP002688 Genomic DNA. Translation: AED97083.1.
RefSeqiNP_568881.1. NM_125254.1.
UniGeneiAt.10551.

3D structure databases

ProteinModelPortaliQ39032.
SMRiQ39032. Positions 78-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21225. 1 interaction.

Proteomic databases

PaxDbiQ39032.
PRIDEiQ39032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58670.1; AT5G58670.1; AT5G58670.
GeneIDi835981.
KEGGiath:AT5G58670.

Organism-specific databases

TAIRiAT5G58670.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ39032.
KOiK05857.
OMAiTHADINY.
PhylomeDBiQ39032.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1622.
BRENDAi3.1.4.11. 399.
ReactomeiREACT_278077. Role of phospholipids in phagocytosis.
REACT_278842. Role of second messengers in netrin-1 signaling.
REACT_279237. VEGFR2 mediated cell proliferation.
REACT_284166. Generation of second messenger molecules.
REACT_286162. PLC beta mediated events.
REACT_306790. GPVI-mediated activation cascade.
REACT_328347. DAG and IP3 signaling.
REACT_350143. Synthesis of IP3 and IP4 in the cytosol.
REACT_361272. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

PROiQ39032.

Gene expression databases

GenevestigatoriQ39032.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana."
    Hirayama T., Ohto C., Mizoguchi T., Shinozaki K.
    Proc. Natl. Acad. Sci. U.S.A. 92:3903-3907(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Columbia.
  2. "The Arabidopsis thaliana genome has multiple divergent forms of phosphoinositol-specific phospholipase C1."
    Hartweck L.M., Llewellyn D.J., Dennis E.S.
    Gene 202:151-156(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. C24.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis PLC1 is required for secondary responses to abscisic acid signals."
    Sanchez J.-P., Chua N.-H.
    Plant Cell 13:1143-1154(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
    Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
    New Phytol. 162:643-654(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCD1_ARATH
AccessioniPrimary (citable) accession number: Q39032
Secondary accession number(s): O49970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.