Phosphoinositide phospholipase C 1 - Arabidopsis thaliana (Mouse-ear cress)

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Q39032 (PLCD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoinositide phospholipase C 1
EC=3.1.4.11

Alternative name(s):
Phosphoinositide phospholipase PLC1
Short name=AtPLC1
Short name=AtPLC1S
Short name=PI-PLC1

Gene names

Name:	PLC1
Synonyms:	ATHATPLC1G
Ordered Locus Names:	At5g58670
ORF Names:	MZN1.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for secondary responses to abscisic acid signals. Ref.1 Ref.5 Ref.7
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in stems, leaves, roots, flowers and siliques. Predominant in the vascular tissues of roots and leaves. Ref.1 Ref.7
Induction	By abscisic acid, osmotic stress and environmental stresses such as dehydration, salinity and low temperature. Ref.1 Ref.7
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cell membrane Membrane
Molecular function	Hydrolase Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid mediated signaling pathway Traceable author statement Ref.5. Source: TAIR intracellular signal transduction Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to cold Inferred from expression pattern Ref.1. Source: TAIR response to salt stress Inferred from expression pattern Ref.1. Source: TAIR response to water deprivation Inferred from expression pattern Ref.1. Source: TAIR
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphatidylinositol phospholipase C activity Inferred from electronic annotation. Source: EC phospholipase C activity Inferred from direct assay Ref.1. Source: TAIR signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 561

561

Phosphoinositide phospholipase C 1

PRO_0000324126

Regions

Domain

21 – 54

EF-hand

Domain

105 – 249

145

PI-PLC X-box

Domain

294 – 410

117

PI-PLC Y-box

Domain

417 – 524

108

Sites

Active site

120

By similarity

Active site

166

By similarity

Experimental info

Sequence conflict

R → G in AAC05023. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q39032 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A05AD123411352E8
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FASTA

561

64,314

        10         20         30         40         50         60 
MKESFKVCFC CVRNFKVKSS EPPEEIKNLF HDYSQDDRMS ADEMLRFVIQ VQGETHADIN 

        70         80         90        100        110        120 
YVKDIFHRLK HHGVFHPRGI HLEGFYRYLL SDFNSPLPLT REVWQDMNQP LSHYFLYTGH 

       130        140        150        160        170        180 
NSYLTGNQLN SNSSIEPIVK ALRNGVRVIE LDLWPNSSGK EAEVRHGGTL TSREDLQKCL 

       190        200        210        220        230        240 
NVVKENAFQV SAYPVVLTLE DHLTPILQKK VAKMVSKTFG GSLFQCTDET TECFPSPESL 

       250        260        270        280        290        300 
KNKILISTKP PKEYLQTQIS KGSTTDESTR AKKISDAEEQ VQEEDEESVA IEYRDLISIH 

       310        320        330        340        350        360 
AGNRKGGLKN CLNGDPNRVI RLSMSEQWLE TLAKTRGPDL VKFTQRNLLR IFPKTTRFDS 

       370        380        390        400        410        420 
SNYDPLVGWI HGAQMVAFNM QSHGRYLWMM QGMFKANGGC GYVKKPDVLL SNGPEGEIFD 

       430        440        450        460        470        480 
PCSQNLPIKT TLKVKIYTGE GWNMDFPLDH FDRYSPPDFY AKVGIAGVPL DTASYRTEID 

       490        500        510        520        530        540 
KDEWFPIWDK EFEFPLRVPE LSLLCITVKD YDSNTQNDFA GQTCFPLSEV RPGIRAVRLH 

       550        560 
DRAGEVYKHV RLLMRFVLEP R

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana." Hirayama T., Ohto C., Mizoguchi T., Shinozaki K. Proc. Natl. Acad. Sci. U.S.A. 92:3903-3907(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[2]	"The Arabidopsis thaliana genome has multiple divergent forms of phosphoinositol-specific phospholipase C1." Hartweck L.M., Llewellyn D.J., Dennis E.S. Gene 202:151-156(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. C24.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Arabidopsis PLC1 is required for secondary responses to abscisic acid signals." Sanchez J.-P., Chua N.-H. Plant Cell 13:1143-1154(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms." Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E. New Phytol. 162:643-654(2004) [AGRICOLA] [Europe PMC] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38544 mRNA. Translation: BAA07547.1. U76423 Genomic DNA. Translation: AAC05023.1. AB020755 Genomic DNA. Translation: BAA97336.1. CP002688 Genomic DNA. Translation: AED97083.1.
IPI	IPI00529107.
RefSeq	NP_568881.1. NM_125254.1.
UniGene	At.10551.
3D structure databases
HSSP	HSSP built from PDB template 1DJH based on UniProtKB P10688.
ProteinModelPortal	Q39032.
SMR	Q39032. Positions 78-559.
ModBase	Search...
Proteomic databases
PaxDb	Q39032.
PRIDE	Q39032.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G58670.1; AT5G58670.1; AT5G58670.
GeneID	835981.
KEGG	ath:AT5G58670.
Organism-specific databases
TAIR	At5g58670.
Phylogenomic databases
eggNOG	NOG149692.
HOGENOM	HOG000244119.
InParanoid	Q39032.
KO	K05857.
OMA	TIWHESH.
PhylomeDB	Q39032.
ProtClustDB	PLN02228.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-1622.
Gene expression databases
Genevestigator	Q39032.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 3.20.20.190. 2 hits.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR001192. Pinositol_PLipase_C. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR015359. PLipase_C_EF-hand-like. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR001711. PLipase_C_Pinositol-sp_Y. [Graphical view]
PANTHER	PTHR10336. PTHR10336. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. False negative. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCD1_ARATH

Accession

Primary (citable) accession number: Q39032
Secondary accession number(s): O49970

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents