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Protein

Serine/threonine-protein kinase AtPK2/AtPK19

Gene

ATPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector of TOR signaling pathway. May be involved in adaptation of plant to cold or high-salt conditions.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by PDK1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei169 – 1691ATPPROSITE-ProRule annotation
Active sitei263 – 2631Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi146 – 1549ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • positive regulation of translation Source: TAIR
  • protein phosphorylation Source: TAIR
  • response to cold Source: TAIR
  • response to heat Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G08720-MONOMER.
ARA:GQT-1970-MONOMER.
BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-166208. mTORC1-mediated signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase AtPK2/AtPK19 (EC:2.7.11.1)
Alternative name(s):
Ribosomal-protein S6 kinase homolog 2
Gene namesi
Name:ATPK2
Synonyms:ATPK19, S6K2
Ordered Locus Names:At3g08720
ORF Names:F17O14.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G08720.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Serine/threonine-protein kinase AtPK2/AtPK19PRO_0000086162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei296 – 2961Phosphoserine; by PDPK11 Publication
Modified residuei455 – 4551Phosphothreonine; by TOR1 Publication

Post-translational modificationi

Undergoes serine-specific autophosphorylation (By similarity). Phosphorylated at Thr-455 by TOR.By similarity2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39030.
PRIDEiQ39030.

PTM databases

iPTMnetiQ39030.

Expressioni

Gene expression databases

ExpressionAtlasiQ39030. baseline and differential.
GenevisibleiQ39030. AT.

Interactioni

Protein-protein interaction databases

BioGridi5354. 2 interactions.
STRINGi3702.AT3G08720.1.

Structurei

3D structure databases

ProteinModelPortaliQ39030.
SMRiQ39030. Positions 135-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini140 – 395256Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini396 – 46671AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni281 – 30727Activation loopBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation.By similarity

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
InParanoidiQ39030.
KOiK04688.
OMAiVANKNQT.
OrthoDBiEOG093608J6.
PhylomeDBiQ39030.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSQCSVAN KNQTGKPFQK HLSLSISPPK SVLGDNLELQ FSDVFGPMPE
60 70 80 90 100
ANSEEACDVA YDEPAVVYSR SHSLVGPSLV VSHSLKMNKL TLRETEDSVD
110 120 130 140 150
LVECVEGESI KENDEFSGND DTDSEKSPEE VSGVVGIEDF EVLKVVGQGA
160 170 180 190 200
FGKVYQVRKK DTSEIYAMKV MRKDKIVEKN HAEYMKAERD ILTKIDHPFI
210 220 230 240 250
VQLKYSFQTK YRLYLVLDFI NGGHLFFQLY HQGLFREDLA RVYTAEIVSA
260 270 280 290 300
VSHLHEKGIM HRDLKPENIL MDVDGHVMLT DFGLAKEFEE NTRSNSMCGT
310 320 330 340 350
TEYMAPEIVR GKGHDKAADW WSVGILLYEM LTGKPPFLGS KGKIQQKIVK
360 370 380 390 400
DKIKLPQFLS NEAHALLKGL LQKEPERRLG SGPSGAEEIK KHKWFKAINW
410 420 430 440 450
KKLEAREVQP SFKPAVSGRQ CIANFDKCWT DMSVLDSPAS SPNSDAKANP
460 470
FTNFTYVRPP HSFLHRTTSN L
Length:471
Mass (Da):53,037
Last modified:June 6, 2002 - v2
Checksum:i95F007B44B58DFB5
GO

Sequence cautioni

The sequence AY050826 differs from that shown. Reason: Frameshift at position 391. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti250 – 2501A → V in BAA07661 (PubMed:7828736).Curated
Sequence conflicti359 – 3602LS → VF in BAA07661 (PubMed:7828736).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42061 mRNA. Translation: BAA07661.1.
AC012562 Genomic DNA. Translation: AAG51345.1.
CP002686 Genomic DNA. Translation: AEE74669.1.
CP002686 Genomic DNA. Translation: AEE74670.1.
AF325094 mRNA. Translation: AAK17162.1.
AY050826 mRNA. No translation available.
PIRiS68463.
RefSeqiNP_187484.1. NM_111706.3.
NP_850543.1. NM_180212.1.
UniGeneiAt.266.

Genome annotation databases

EnsemblPlantsiAT3G08720.1; AT3G08720.1; AT3G08720.
AT3G08720.2; AT3G08720.2; AT3G08720.
GeneIDi820019.
GrameneiAT3G08720.1; AT3G08720.1; AT3G08720.
AT3G08720.2; AT3G08720.2; AT3G08720.
KEGGiath:AT3G08720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42061 mRNA. Translation: BAA07661.1.
AC012562 Genomic DNA. Translation: AAG51345.1.
CP002686 Genomic DNA. Translation: AEE74669.1.
CP002686 Genomic DNA. Translation: AEE74670.1.
AF325094 mRNA. Translation: AAK17162.1.
AY050826 mRNA. No translation available.
PIRiS68463.
RefSeqiNP_187484.1. NM_111706.3.
NP_850543.1. NM_180212.1.
UniGeneiAt.266.

3D structure databases

ProteinModelPortaliQ39030.
SMRiQ39030. Positions 135-452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5354. 2 interactions.
STRINGi3702.AT3G08720.1.

PTM databases

iPTMnetiQ39030.

Proteomic databases

PaxDbiQ39030.
PRIDEiQ39030.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G08720.1; AT3G08720.1; AT3G08720.
AT3G08720.2; AT3G08720.2; AT3G08720.
GeneIDi820019.
GrameneiAT3G08720.1; AT3G08720.1; AT3G08720.
AT3G08720.2; AT3G08720.2; AT3G08720.
KEGGiath:AT3G08720.

Organism-specific databases

TAIRiAT3G08720.

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
InParanoidiQ39030.
KOiK04688.
OMAiVANKNQT.
OrthoDBiEOG093608J6.
PhylomeDBiQ39030.

Enzyme and pathway databases

BioCyciARA:AT3G08720-MONOMER.
ARA:GQT-1970-MONOMER.
BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-166208. mTORC1-mediated signalling.

Miscellaneous databases

PROiQ39030.

Gene expression databases

ExpressionAtlasiQ39030. baseline and differential.
GenevisibleiQ39030. AT.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPK2_ARATH
AccessioniPrimary (citable) accession number: Q39030
Secondary accession number(s): Q949X5, Q9C5R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 6, 2002
Last modified: September 7, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.