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Protein

Mitogen-activated protein kinase 7

Gene

MPK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MKK3-MPK7 module acts as a positive regulator of PR1 gene expression.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated in response to hydrogen peroxide.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ATPPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. MAPK cascade Source: GOC
  3. response to hydrogen peroxide Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G18170-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiREACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 7 (EC:2.7.11.24)
Short name:
AtMPK7
Short name:
MAP kinase 7
Gene namesi
Name:MPK7
Ordered Locus Names:At2g18170
ORF Names:F8D23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G18170.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Mitogen-activated protein kinase 7PRO_0000186316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphothreonineBy similarity
Modified residuei193 – 1931PhosphotyrosineBy similarity
Modified residuei196 – 1961PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39027.
PRIDEiQ39027.

Expressioni

Gene expression databases

ExpressionAtlasiQ39027. baseline and differential.
GenevestigatoriQ39027.

Interactioni

Subunit structurei

Interacts with MKK3.2 Publications

Protein-protein interaction databases

IntActiQ39027. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ39027.
SMRiQ39027. Positions 26-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ39027.
KOiK08293.
OMAiMLVEPPN.
PhylomeDBiQ39027.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39027-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMLVEPPNG IKQQGKHYYS MWQTLFEIDT KYVPIKPIGR GAYGVVCSSI
60 70 80 90 100
NRETNERVAI KKIHNVFENR VDALRTLREL KLLRHVRHEN VIALKDVMLP
110 120 130 140 150
ANRSSFKDVY LVYELMDTDL HQIIKSSQSL SDDHCKYFLF QLLRGLKYLH
160 170 180 190 200
SANILHRDLK PGNLLVNANC DLKICDFGLA RTSQGNEQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAEILGR KPIFPGTECL NQLKLIINVV
260 270 280 290 300
GSQQESDIRF IDNPKARRFI KSLPYSRGTH LSNLYPQANP LAIDLLQRML
310 320 330 340 350
VFDPTKRISV TDALLHPYMA GLFDPGSNPP AHVPISLDID ENMEEPVIRE
360
MMWNEMLYYH PEAEISNA
Length:368
Mass (Da):42,299
Last modified:June 6, 2002 - v2
Checksum:i64587E4CA40C1418
GO

Sequence cautioni

The sequence BAD95376.1 differs from that shown. Reason: Frameshift at position 50. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041S → T in BAA04870 (PubMed:8282107).Curated
Sequence conflicti303 – 3031D → E in BAA04870 (PubMed:8282107).Curated
Sequence conflicti324 – 3241D → E in BAA04870 (PubMed:8282107).Curated
Sequence conflicti327 – 3271S → T in BAA04870 (PubMed:8282107).Curated
Sequence conflicti366 – 3661S → L in BAA04870 (PubMed:8282107).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21843 mRNA. Translation: BAA04870.1.
AC007212 Genomic DNA. Translation: AAD31349.1.
CP002685 Genomic DNA. Translation: AEC06734.1.
AK222214 mRNA. Translation: BAD95376.1. Frameshift.
PIRiB84561.
S40473.
RefSeqiNP_179409.1. NM_127374.3.
UniGeneiAt.265.
At.68138.

Genome annotation databases

EnsemblPlantsiAT2G18170.1; AT2G18170.1; AT2G18170.
GeneIDi816330.
KEGGiath:AT2G18170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21843 mRNA. Translation: BAA04870.1.
AC007212 Genomic DNA. Translation: AAD31349.1.
CP002685 Genomic DNA. Translation: AEC06734.1.
AK222214 mRNA. Translation: BAD95376.1. Frameshift.
PIRiB84561.
S40473.
RefSeqiNP_179409.1. NM_127374.3.
UniGeneiAt.265.
At.68138.

3D structure databases

ProteinModelPortaliQ39027.
SMRiQ39027. Positions 26-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ39027. 2 interactions.

Proteomic databases

PaxDbiQ39027.
PRIDEiQ39027.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G18170.1; AT2G18170.1; AT2G18170.
GeneIDi816330.
KEGGiath:AT2G18170.

Organism-specific databases

GeneFarmi812. 89.
TAIRiAT2G18170.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ39027.
KOiK08293.
OMAiMLVEPPN.
PhylomeDBiQ39027.

Enzyme and pathway databases

BioCyciARA:AT2G18170-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiREACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Gene expression databases

ExpressionAtlasiQ39027. baseline and differential.
GenevestigatoriQ39027.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana."
    Mizoguchi T., Hayashida N., Yamaguchi-Shinozaki K., Kamada H., Shinozaki K.
    FEBS Lett. 336:440-444(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: GENE FAMILY.
  7. "The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling."
    Doczi R., Brader G., Pettko-Szandtner A., Rajh I., Djamei A., Pitzschke A., Teige M., Hirt H.
    Plant Cell 19:3266-3279(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK3, ENZYME REGULATION, FUNCTION.
  8. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
    Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
    Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK3.

Entry informationi

Entry nameiMPK7_ARATH
AccessioniPrimary (citable) accession number: Q39027
Secondary accession number(s): Q56W33, Q9SI14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: March 4, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.