Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q39027

- MPK7_ARATH

UniProt

Q39027 - MPK7_ARATH

Protein

Mitogen-activated protein kinase 7

Gene

MPK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (06 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    MKK3-MPK7 module acts as a positive regulator of PR1 gene expression.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to hydrogen peroxide.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611ATPPROSITE-ProRule annotation
    Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: TAIR
    3. protein binding Source: TAIR

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. MAPK cascade Source: GOC
    3. response to hydrogen peroxide Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Plant defense

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G18170-MONOMER.
    BRENDAi2.7.11.24. 399.
    ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 7 (EC:2.7.11.24)
    Short name:
    AtMPK7
    Short name:
    MAP kinase 7
    Gene namesi
    Name:MPK7
    Ordered Locus Names:At2g18170
    ORF Names:F8D23
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G18170.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 368368Mitogen-activated protein kinase 7PRO_0000186316Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911PhosphothreonineBy similarity
    Modified residuei193 – 1931PhosphotyrosineBy similarity
    Modified residuei196 – 1961PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ39027.
    PRIDEiQ39027.

    Expressioni

    Gene expression databases

    GenevestigatoriQ39027.

    Interactioni

    Subunit structurei

    Interacts with MKK3.2 Publications

    Protein-protein interaction databases

    IntActiQ39027. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39027.
    SMRiQ39027. Positions 15-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi191 – 1933TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ39027.
    KOiK08293.
    OMAiMYPQAHP.
    PhylomeDBiQ39027.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q39027-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMLVEPPNG IKQQGKHYYS MWQTLFEIDT KYVPIKPIGR GAYGVVCSSI    50
    NRETNERVAI KKIHNVFENR VDALRTLREL KLLRHVRHEN VIALKDVMLP 100
    ANRSSFKDVY LVYELMDTDL HQIIKSSQSL SDDHCKYFLF QLLRGLKYLH 150
    SANILHRDLK PGNLLVNANC DLKICDFGLA RTSQGNEQFM TEYVVTRWYR 200
    APELLLCCDN YGTSIDVWSV GCIFAEILGR KPIFPGTECL NQLKLIINVV 250
    GSQQESDIRF IDNPKARRFI KSLPYSRGTH LSNLYPQANP LAIDLLQRML 300
    VFDPTKRISV TDALLHPYMA GLFDPGSNPP AHVPISLDID ENMEEPVIRE 350
    MMWNEMLYYH PEAEISNA 368
    Length:368
    Mass (Da):42,299
    Last modified:June 6, 2002 - v2
    Checksum:i64587E4CA40C1418
    GO

    Sequence cautioni

    The sequence BAD95376.1 differs from that shown. Reason: Frameshift at position 50.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti104 – 1041S → T in BAA04870. (PubMed:8282107)Curated
    Sequence conflicti303 – 3031D → E in BAA04870. (PubMed:8282107)Curated
    Sequence conflicti324 – 3241D → E in BAA04870. (PubMed:8282107)Curated
    Sequence conflicti327 – 3271S → T in BAA04870. (PubMed:8282107)Curated
    Sequence conflicti366 – 3661S → L in BAA04870. (PubMed:8282107)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21843 mRNA. Translation: BAA04870.1.
    AC007212 Genomic DNA. Translation: AAD31349.1.
    CP002685 Genomic DNA. Translation: AEC06734.1.
    AK222214 mRNA. Translation: BAD95376.1. Frameshift.
    PIRiB84561.
    S40473.
    RefSeqiNP_179409.1. NM_127374.3.
    UniGeneiAt.265.
    At.68138.

    Genome annotation databases

    EnsemblPlantsiAT2G18170.1; AT2G18170.1; AT2G18170.
    GeneIDi816330.
    KEGGiath:AT2G18170.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21843 mRNA. Translation: BAA04870.1 .
    AC007212 Genomic DNA. Translation: AAD31349.1 .
    CP002685 Genomic DNA. Translation: AEC06734.1 .
    AK222214 mRNA. Translation: BAD95376.1 . Frameshift.
    PIRi B84561.
    S40473.
    RefSeqi NP_179409.1. NM_127374.3.
    UniGenei At.265.
    At.68138.

    3D structure databases

    ProteinModelPortali Q39027.
    SMRi Q39027. Positions 15-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q39027. 2 interactions.

    Proteomic databases

    PaxDbi Q39027.
    PRIDEi Q39027.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G18170.1 ; AT2G18170.1 ; AT2G18170 .
    GeneIDi 816330.
    KEGGi ath:AT2G18170.

    Organism-specific databases

    GeneFarmi 812. 89.
    TAIRi AT2G18170.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q39027.
    KOi K08293.
    OMAi MYPQAHP.
    PhylomeDBi Q39027.

    Enzyme and pathway databases

    BioCyci ARA:AT2G18170-MONOMER.
    BRENDAi 2.7.11.24. 399.
    Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Gene expression databases

    Genevestigatori Q39027.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana."
      Mizoguchi T., Hayashida N., Yamaguchi-Shinozaki K., Kamada H., Shinozaki K.
      FEBS Lett. 336:440-444(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. Cited for: GENE FAMILY.
    7. "The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling."
      Doczi R., Brader G., Pettko-Szandtner A., Rajh I., Djamei A., Pitzschke A., Teige M., Hirt H.
      Plant Cell 19:3266-3279(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKK3, ENZYME REGULATION, FUNCTION.
    8. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
      Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
      Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKK3.

    Entry informationi

    Entry nameiMPK7_ARATH
    AccessioniPrimary (citable) accession number: Q39027
    Secondary accession number(s): Q56W33, Q9SI14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: June 6, 2002
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3