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Q39027 (MPK7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 7

Short name=AtMPK7
Short name=MAP kinase 7
EC=2.7.11.24
Gene names
Name:MPK7
Ordered Locus Names:At2g18170
ORF Names:F8D23
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

MKK3-MPK7 module acts as a positive regulator of PR1 gene expression. Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to hydrogen peroxide. Ref.7

Subunit structure

Interacts with MKK3. Ref.7 Ref.8

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAD95376.1 differs from that shown. Reason: Frameshift at position 50.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368Mitogen-activated protein kinase 7
PRO_0000186316

Regions

Domain32 – 319288Protein kinase
Nucleotide binding38 – 469ATP By similarity
Motif191 – 1933TXY

Sites

Active site1581Proton acceptor By similarity
Binding site611ATP By similarity

Amino acid modifications

Modified residue1911Phosphothreonine By similarity
Modified residue1931Phosphotyrosine By similarity
Modified residue1961Phosphothreonine By similarity

Experimental info

Sequence conflict1041S → T in BAA04870. Ref.1
Sequence conflict3031D → E in BAA04870. Ref.1
Sequence conflict3241D → E in BAA04870. Ref.1
Sequence conflict3271S → T in BAA04870. Ref.1
Sequence conflict3661S → L in BAA04870. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q39027 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: 64587E4CA40C1418

FASTA36842,299
        10         20         30         40         50         60 
MAMLVEPPNG IKQQGKHYYS MWQTLFEIDT KYVPIKPIGR GAYGVVCSSI NRETNERVAI 

        70         80         90        100        110        120 
KKIHNVFENR VDALRTLREL KLLRHVRHEN VIALKDVMLP ANRSSFKDVY LVYELMDTDL 

       130        140        150        160        170        180 
HQIIKSSQSL SDDHCKYFLF QLLRGLKYLH SANILHRDLK PGNLLVNANC DLKICDFGLA 

       190        200        210        220        230        240 
RTSQGNEQFM TEYVVTRWYR APELLLCCDN YGTSIDVWSV GCIFAEILGR KPIFPGTECL 

       250        260        270        280        290        300 
NQLKLIINVV GSQQESDIRF IDNPKARRFI KSLPYSRGTH LSNLYPQANP LAIDLLQRML 

       310        320        330        340        350        360 
VFDPTKRISV TDALLHPYMA GLFDPGSNPP AHVPISLDID ENMEEPVIRE MMWNEMLYYH 


PEAEISNA 

« Hide

References

« Hide 'large scale' references
[1]"ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana."
Mizoguchi T., Hayashida N., Yamaguchi-Shinozaki K., Kamada H., Shinozaki K.
FEBS Lett. 336:440-444(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[7]"The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling."
Doczi R., Brader G., Pettko-Szandtner A., Rajh I., Djamei A., Pitzschke A., Teige M., Hirt H.
Plant Cell 19:3266-3279(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK3, ENZYME REGULATION, FUNCTION.
[8]"Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D21843 mRNA. Translation: BAA04870.1.
AC007212 Genomic DNA. Translation: AAD31349.1.
CP002685 Genomic DNA. Translation: AEC06734.1.
AK222214 mRNA. Translation: BAD95376.1. Frameshift.
PIRB84561.
S40473.
RefSeqNP_179409.1. NM_127374.3.
UniGeneAt.265.
At.68138.

3D structure databases

ProteinModelPortalQ39027.
SMRQ39027. Positions 15-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ39027. 2 interactions.

Proteomic databases

PaxDbQ39027.
PRIDEQ39027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G18170.1; AT2G18170.1; AT2G18170.
GeneID816330.
KEGGath:AT2G18170.

Organism-specific databases

GeneFarm812. 89.
TAIRAT2G18170.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ39027.
KOK08293.
OMAMYPQAHP.
PhylomeDBQ39027.

Enzyme and pathway databases

BioCycARA:AT2G18170-MONOMER.
BRENDA2.7.11.24. 399.

Gene expression databases

GenevestigatorQ39027.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK7_ARATH
AccessionPrimary (citable) accession number: Q39027
Secondary accession number(s): Q56W33, Q9SI14
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names