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Q39027

- MPK7_ARATH

UniProt

Q39027 - MPK7_ARATH

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Protein
Mitogen-activated protein kinase 7
Gene
MPK7, At2g18170, F8D23
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

MKK3-MPK7 module acts as a positive regulator of PR1 gene expression.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to hydrogen peroxide.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ATP By similarity
Active sitei158 – 1581Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR
  3. protein binding Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. MAPK cascade Source: GOC
  2. defense response Source: UniProtKB-KW
  3. response to hydrogen peroxide Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G18170-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 7 (EC:2.7.11.24)
Short name:
AtMPK7
Short name:
MAP kinase 7
Gene namesi
Name:MPK7
Ordered Locus Names:At2g18170
ORF Names:F8D23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G18170.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Mitogen-activated protein kinase 7
PRO_0000186316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911Phosphothreonine By similarity
Modified residuei193 – 1931Phosphotyrosine By similarity
Modified residuei196 – 1961Phosphothreonine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39027.
PRIDEiQ39027.

Expressioni

Gene expression databases

GenevestigatoriQ39027.

Interactioni

Subunit structurei

Interacts with MKK3.2 Publications

Protein-protein interaction databases

IntActiQ39027. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ39027.
SMRiQ39027. Positions 15-365.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 319288Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ39027.
KOiK08293.
OMAiMYPQAHP.
PhylomeDBiQ39027.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39027-1 [UniParc]FASTAAdd to Basket

« Hide

MAMLVEPPNG IKQQGKHYYS MWQTLFEIDT KYVPIKPIGR GAYGVVCSSI    50
NRETNERVAI KKIHNVFENR VDALRTLREL KLLRHVRHEN VIALKDVMLP 100
ANRSSFKDVY LVYELMDTDL HQIIKSSQSL SDDHCKYFLF QLLRGLKYLH 150
SANILHRDLK PGNLLVNANC DLKICDFGLA RTSQGNEQFM TEYVVTRWYR 200
APELLLCCDN YGTSIDVWSV GCIFAEILGR KPIFPGTECL NQLKLIINVV 250
GSQQESDIRF IDNPKARRFI KSLPYSRGTH LSNLYPQANP LAIDLLQRML 300
VFDPTKRISV TDALLHPYMA GLFDPGSNPP AHVPISLDID ENMEEPVIRE 350
MMWNEMLYYH PEAEISNA 368
Length:368
Mass (Da):42,299
Last modified:June 6, 2002 - v2
Checksum:i64587E4CA40C1418
GO

Sequence cautioni

The sequence BAD95376.1 differs from that shown. Reason: Frameshift at position 50.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041S → T in BAA04870. 1 Publication
Sequence conflicti303 – 3031D → E in BAA04870. 1 Publication
Sequence conflicti324 – 3241D → E in BAA04870. 1 Publication
Sequence conflicti327 – 3271S → T in BAA04870. 1 Publication
Sequence conflicti366 – 3661S → L in BAA04870. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D21843 mRNA. Translation: BAA04870.1.
AC007212 Genomic DNA. Translation: AAD31349.1.
CP002685 Genomic DNA. Translation: AEC06734.1.
AK222214 mRNA. Translation: BAD95376.1. Frameshift.
PIRiB84561.
S40473.
RefSeqiNP_179409.1. NM_127374.3.
UniGeneiAt.265.
At.68138.

Genome annotation databases

EnsemblPlantsiAT2G18170.1; AT2G18170.1; AT2G18170.
GeneIDi816330.
KEGGiath:AT2G18170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D21843 mRNA. Translation: BAA04870.1 .
AC007212 Genomic DNA. Translation: AAD31349.1 .
CP002685 Genomic DNA. Translation: AEC06734.1 .
AK222214 mRNA. Translation: BAD95376.1 . Frameshift.
PIRi B84561.
S40473.
RefSeqi NP_179409.1. NM_127374.3.
UniGenei At.265.
At.68138.

3D structure databases

ProteinModelPortali Q39027.
SMRi Q39027. Positions 15-365.
ModBasei Search...

Protein-protein interaction databases

IntActi Q39027. 2 interactions.

Proteomic databases

PaxDbi Q39027.
PRIDEi Q39027.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G18170.1 ; AT2G18170.1 ; AT2G18170 .
GeneIDi 816330.
KEGGi ath:AT2G18170.

Organism-specific databases

GeneFarmi 812. 89.
TAIRi AT2G18170.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q39027.
KOi K08293.
OMAi MYPQAHP.
PhylomeDBi Q39027.

Enzyme and pathway databases

BioCyci ARA:AT2G18170-MONOMER.
BRENDAi 2.7.11.24. 399.
Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Gene expression databases

Genevestigatori Q39027.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana."
    Mizoguchi T., Hayashida N., Yamaguchi-Shinozaki K., Kamada H., Shinozaki K.
    FEBS Lett. 336:440-444(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: GENE FAMILY.
  7. "The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling."
    Doczi R., Brader G., Pettko-Szandtner A., Rajh I., Djamei A., Pitzschke A., Teige M., Hirt H.
    Plant Cell 19:3266-3279(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK3, ENZYME REGULATION, FUNCTION.
  8. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
    Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
    Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK3.

Entry informationi

Entry nameiMPK7_ARATH
AccessioniPrimary (citable) accession number: Q39027
Secondary accession number(s): Q56W33, Q9SI14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: September 3, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi