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Protein

Mitogen-activated protein kinase 6

Gene

MPK6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Phosphorylates 1-aminocyclopropane-1-carboxylic acid synthases (ACS2 and ACS6) and may be involved in the regulation of bacterial elicitor flagellin-induced ethylene production. Regulates locally gene-mediated and basal resistance response to certain pathogens. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination. MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. MKK9-MPK6 module positively regulates leaf senescence. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.14 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Activated by the MAP kinase kinases MKK2, MKK3, MKK4, MKK5, MKK7 and MKK9. Activated in response to touch, wounding, low temperature, low humidity, salt stress, hydrogen peroxide, ozone, ACC (an ethylene precursor), jasmonic acid (JA), mastoparan and UVC. Activated in response to elicitors: oligogalacturonides, hexameric chitin fragments, fungal xylanase, and the bacterial flagellin and harpin. Activated upon Pseudomonas syringae pv. tomato DC3000 infection. Repressed by the protein phosphatase 2C AP2C1 and the protein-tyrosine-phosphatases MKP1 and PTP1. Repressed by DSPTP1B/MKP2-mediated dephosphorylation.18 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921ATP
Active sitei189 – 1891Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 779ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR
  • protein kinase activity Source: TAIR

GO - Biological processi

  • camalexin biosynthetic process Source: TAIR
  • cell division Source: TAIR
  • defense response to bacterium Source: TAIR
  • induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • inflorescence development Source: TAIR
  • leaf senescence Source: UniProtKB
  • plant ovule development Source: TAIR
  • plant-type hypersensitive response Source: UniProtKB-KW
  • pollen development Source: TAIR
  • pollen tube guidance Source: TAIR
  • priming of cellular response to stress Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to ethylene Source: TAIR
  • response to fungus Source: UniProtKB
  • response to hydrogen peroxide Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to reactive oxygen species Source: TAIR
  • response to salt stress Source: TAIR
  • response to UV-B Source: TAIR
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Hypersensitive response, Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G43790-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 6 (EC:2.7.11.24)
Short name:
AtMPK6
Short name:
MAP kinase 6
Gene namesi
Name:MPK6
Ordered Locus Names:At2g43790
ORF Names:F18O19.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G43790.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Translocated into the nucleus in response to phosphorylation.Curated

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • phragmoplast Source: TAIR
  • preprophase band Source: TAIR
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced sensitivity to abscisic acid (ABA) during germination. Delayed senescence phenotype.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi92 – 921K → M: Loss of kinase activity. 1 Publication
Mutagenesisi93 – 931K → M: Loss of kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Mitogen-activated protein kinase 6PRO_0000186315Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei221 – 2211Phosphothreonine1 Publication
Modified residuei223 – 2231Phosphotyrosine1 Publication
Modified residuei226 – 2261PhosphothreonineCombined sources

Post-translational modificationi

Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Dephosphorylated by DSPTP1B/MKP2.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39026.
PRIDEiQ39026.

PTM databases

iPTMnetiQ39026.

Expressioni

Inductioni

By Alternaria brassicae pathogen infection.1 Publication

Gene expression databases

GenevisibleiQ39026. AT.

Interactioni

Subunit structurei

Interacts with MEKK1, MKK1 and MKK2. May form a ternary complex with MEKK1 and MKK1 or MKK2. Interacts with NDPK2, AP2C1, MKP1 and PTP1. Interacts with DSPTP1B/MKP2, especially during HR-like responses triggered by fungal elicitors. Interacts with MKK4, MKK5 and MKK6 (PubMed:12506203, PubMed:15225555, PubMed:17630279, PubMed:19513235, PubMed:19789277, PubMed:20626661, PubMed:21057191, PubMed:21575092). Binds to LIP5 (PubMed:25010425). Interacts with VQ4 and IKU1/VQ14 (PubMed:24750137). Interacts with RACK1A, RACK1B and RACK1C (PubMed:25731164).11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
At2g47060O807193EBI-349548,EBI-4436376
ERF104Q9FKG13EBI-349548,EBI-2360943
MKK2Q9S7U95EBI-349548,EBI-994350

Protein-protein interaction databases

BioGridi4318. 32 interactions.
DIPiDIP-31825N.
IntActiQ39026. 12 interactions.
MINTiMINT-8086250.
STRINGi3702.AT2G43790.1.

Structurei

Secondary structure

1
395
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 363Combined sources
Beta strandi38 – 414Combined sources
Turni42 – 454Combined sources
Beta strandi46 – 494Combined sources
Beta strandi56 – 594Combined sources
Beta strandi73 – 753Combined sources
Beta strandi77 – 826Combined sources
Turni83 – 853Combined sources
Beta strandi88 – 947Combined sources
Beta strandi97 – 1004Combined sources
Helixi101 – 11616Combined sources
Beta strandi125 – 1284Combined sources
Helixi134 – 1363Combined sources
Beta strandi140 – 1456Combined sources
Beta strandi148 – 1503Combined sources
Helixi151 – 1566Combined sources
Helixi163 – 18220Combined sources
Helixi192 – 1943Combined sources
Beta strandi195 – 1973Combined sources
Beta strandi203 – 2053Combined sources
Helixi232 – 2354Combined sources
Helixi244 – 25916Combined sources
Helixi269 – 28012Combined sources
Turni285 – 2884Combined sources
Helixi293 – 3019Combined sources
Helixi310 – 3134Combined sources
Helixi319 – 32810Combined sources
Helixi333 – 3353Combined sources
Helixi339 – 3435Combined sources
Helixi346 – 3483Combined sources
Turni349 – 3513Combined sources
Helixi354 – 3563Combined sources
Beta strandi368 – 3714Combined sources
Helixi378 – 38912Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5CI6X-ray3.00A/B29-395[»]
ProteinModelPortaliQ39026.
SMRiQ39026. Positions 5-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 348286Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi221 – 2233TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39026.
KOiK14512.
OMAiMDGGAQP.
OrthoDBiEOG09360BWL.
PhylomeDBiQ39026.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGGSGQPAA DTEMTEAPGG FPAAAPSPQM PGIENIPATL SHGGRFIQYN
60 70 80 90 100
IFGNIFEVTA KYKPPIMPIG KGAYGIVCSA MNSETNESVA IKKIANAFDN
110 120 130 140 150
KIDAKRTLRE IKLLRHMDHE NIVAIRDIIP PPLRNAFNDV YIAYELMDTD
160 170 180 190 200
LHQIIRSNQA LSEEHCQYFL YQILRGLKYI HSANVLHRDL KPSNLLLNAN
210 220 230 240 250
CDLKICDFGL ARVTSESDFM TEYVVTRWYR APELLLNSSD YTAAIDVWSV
260 270 280 290 300
GCIFMELMDR KPLFPGRDHV HQLRLLMELI GTPSEEELEF LNENAKRYIR
310 320 330 340 350
QLPPYPRQSI TDKFPTVHPL AIDLIEKMLT FDPRRRITVL DALAHPYLNS
360 370 380 390
LHDISDEPEC TIPFNFDFEN HALSEEQMKE LIYREALAFN PEYQQ
Length:395
Mass (Da):45,058
Last modified:November 1, 1996 - v1
Checksum:i296D2BD753C6DDA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21842 mRNA. Translation: BAA04869.1.
AC002333 Genomic DNA. Translation: AAB64027.1.
CP002685 Genomic DNA. Translation: AEC10325.1.
AY120737 mRNA. Translation: AAM53295.1.
BT008855 mRNA. Translation: AAP68294.1.
PIRiS40472.
RefSeqiNP_181907.1. NM_129941.3.
UniGeneiAt.22266.
At.53112.

Genome annotation databases

EnsemblPlantsiAT2G43790.1; AT2G43790.1; AT2G43790.
GeneIDi818982.
GrameneiAT2G43790.1; AT2G43790.1; AT2G43790.
KEGGiath:AT2G43790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21842 mRNA. Translation: BAA04869.1.
AC002333 Genomic DNA. Translation: AAB64027.1.
CP002685 Genomic DNA. Translation: AEC10325.1.
AY120737 mRNA. Translation: AAM53295.1.
BT008855 mRNA. Translation: AAP68294.1.
PIRiS40472.
RefSeqiNP_181907.1. NM_129941.3.
UniGeneiAt.22266.
At.53112.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5CI6X-ray3.00A/B29-395[»]
ProteinModelPortaliQ39026.
SMRiQ39026. Positions 5-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4318. 32 interactions.
DIPiDIP-31825N.
IntActiQ39026. 12 interactions.
MINTiMINT-8086250.
STRINGi3702.AT2G43790.1.

PTM databases

iPTMnetiQ39026.

Proteomic databases

PaxDbiQ39026.
PRIDEiQ39026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43790.1; AT2G43790.1; AT2G43790.
GeneIDi818982.
GrameneiAT2G43790.1; AT2G43790.1; AT2G43790.
KEGGiath:AT2G43790.

Organism-specific databases

TAIRiAT2G43790.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39026.
KOiK14512.
OMAiMDGGAQP.
OrthoDBiEOG09360BWL.
PhylomeDBiQ39026.

Enzyme and pathway databases

BioCyciARA:AT2G43790-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Miscellaneous databases

PROiQ39026.

Gene expression databases

GenevisibleiQ39026. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK6_ARATH
AccessioniPrimary (citable) accession number: Q39026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.