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Protein

Mitogen-activated protein kinase 6

Gene

MPK6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Phosphorylates 1-aminocyclopropane-1-carboxylic acid synthases (ACS2 and ACS6) and may be involved in the regulation of bacterial elicitor flagellin-induced ethylene production. Regulates locally gene-mediated and basal resistance response to certain pathogens. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination. MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. MKK9-MPK6 module positively regulates leaf senescence. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.14 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Activated by the MAP kinase kinases MKK2, MKK3, MKK4, MKK5, MKK7 and MKK9. Activated in response to touch, wounding, low temperature, low humidity, salt stress, hydrogen peroxide, ozone, ACC (an ethylene precursor), jasmonic acid (JA), mastoparan and UVC. Activated in response to elicitors: oligogalacturonides, hexameric chitin fragments, fungal xylanase, and the bacterial flagellin and harpin. Activated upon Pseudomonas syringae pv. tomato DC3000 infection. Repressed by the protein phosphatase 2C AP2C1 and the protein-tyrosine-phosphatases MKP1 and PTP1. Repressed by DSPTP1B/MKP2-mediated dephosphorylation.18 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92ATP1
Active sitei189Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 77ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR
  • protein kinase activity Source: TAIR

GO - Biological processi

  • camalexin biosynthetic process Source: TAIR
  • cell division Source: TAIR
  • defense response to bacterium Source: TAIR
  • induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • inflorescence development Source: TAIR
  • leaf senescence Source: UniProtKB
  • plant ovule development Source: TAIR
  • plant-type hypersensitive response Source: UniProtKB-KW
  • pollen development Source: TAIR
  • pollen tube guidance Source: TAIR
  • priming of cellular response to stress Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to ethylene Source: TAIR
  • response to fungus Source: UniProtKB
  • response to hydrogen peroxide Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to reactive oxygen species Source: TAIR
  • response to salt stress Source: TAIR
  • response to UV-B Source: TAIR
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Hypersensitive response, Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 6 (EC:2.7.11.24)
Short name:
AtMPK6
Short name:
MAP kinase 6
Gene namesi
Name:MPK6
Ordered Locus Names:At2g43790
ORF Names:F18O19.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G43790.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Translocated into the nucleus in response to phosphorylation.Curated

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • phragmoplast Source: TAIR
  • preprophase band Source: TAIR
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced sensitivity to abscisic acid (ABA) during germination. Delayed senescence phenotype.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92K → M: Loss of kinase activity. 1 Publication1
Mutagenesisi93K → M: Loss of kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863151 – 395Mitogen-activated protein kinase 6Add BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei221Phosphothreonine1 Publication1
Modified residuei223Phosphotyrosine1 Publication1
Modified residuei226PhosphothreonineCombined sources1

Post-translational modificationi

Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Dephosphorylated by DSPTP1B/MKP2.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39026.

PTM databases

iPTMnetiQ39026.

Expressioni

Inductioni

By Alternaria brassicae pathogen infection.1 Publication

Gene expression databases

GenevisibleiQ39026. AT.

Interactioni

Subunit structurei

Interacts with MEKK1, MKK1 and MKK2. May form a ternary complex with MEKK1 and MKK1 or MKK2. Interacts with NDPK2, AP2C1, MKP1 and PTP1. Interacts with DSPTP1B/MKP2, especially during HR-like responses triggered by fungal elicitors. Interacts with MKK4, MKK5 and MKK6 (PubMed:12506203, PubMed:15225555, PubMed:17630279, PubMed:19513235, PubMed:19789277, PubMed:20626661, PubMed:21057191, PubMed:21575092). Binds to LIP5 (PubMed:25010425). Interacts with VQ4 and IKU1/VQ14 (PubMed:24750137). Interacts with RACK1A, RACK1B and RACK1C (PubMed:25731164).11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
At2g47060O807193EBI-349548,EBI-4436376
ERF104Q9FKG13EBI-349548,EBI-2360943
MKK2Q9S7U95EBI-349548,EBI-994350

Protein-protein interaction databases

BioGridi4318. 32 interactors.
DIPiDIP-31825N.
IntActiQ39026. 12 interactors.
MINTiMINT-8086250.
STRINGi3702.AT2G43790.1.

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 36Combined sources3
Beta strandi38 – 41Combined sources4
Turni42 – 45Combined sources4
Beta strandi46 – 49Combined sources4
Beta strandi56 – 59Combined sources4
Beta strandi73 – 75Combined sources3
Beta strandi77 – 82Combined sources6
Turni83 – 85Combined sources3
Beta strandi88 – 94Combined sources7
Beta strandi97 – 100Combined sources4
Helixi101 – 116Combined sources16
Beta strandi125 – 128Combined sources4
Helixi134 – 136Combined sources3
Beta strandi140 – 145Combined sources6
Beta strandi148 – 150Combined sources3
Helixi151 – 156Combined sources6
Helixi163 – 182Combined sources20
Helixi192 – 194Combined sources3
Beta strandi195 – 197Combined sources3
Beta strandi203 – 205Combined sources3
Helixi232 – 235Combined sources4
Helixi244 – 259Combined sources16
Helixi269 – 280Combined sources12
Turni285 – 288Combined sources4
Helixi293 – 301Combined sources9
Helixi310 – 313Combined sources4
Helixi319 – 328Combined sources10
Helixi333 – 335Combined sources3
Helixi339 – 343Combined sources5
Helixi346 – 348Combined sources3
Turni349 – 351Combined sources3
Helixi354 – 356Combined sources3
Beta strandi368 – 371Combined sources4
Helixi378 – 389Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CI6X-ray3.00A/B29-395[»]
ProteinModelPortaliQ39026.
SMRiQ39026.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 348Protein kinasePROSITE-ProRule annotationAdd BLAST286

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi221 – 223TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39026.
KOiK14512.
OMAiMDGGAQP.
OrthoDBiEOG09360BWL.
PhylomeDBiQ39026.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGGSGQPAA DTEMTEAPGG FPAAAPSPQM PGIENIPATL SHGGRFIQYN
60 70 80 90 100
IFGNIFEVTA KYKPPIMPIG KGAYGIVCSA MNSETNESVA IKKIANAFDN
110 120 130 140 150
KIDAKRTLRE IKLLRHMDHE NIVAIRDIIP PPLRNAFNDV YIAYELMDTD
160 170 180 190 200
LHQIIRSNQA LSEEHCQYFL YQILRGLKYI HSANVLHRDL KPSNLLLNAN
210 220 230 240 250
CDLKICDFGL ARVTSESDFM TEYVVTRWYR APELLLNSSD YTAAIDVWSV
260 270 280 290 300
GCIFMELMDR KPLFPGRDHV HQLRLLMELI GTPSEEELEF LNENAKRYIR
310 320 330 340 350
QLPPYPRQSI TDKFPTVHPL AIDLIEKMLT FDPRRRITVL DALAHPYLNS
360 370 380 390
LHDISDEPEC TIPFNFDFEN HALSEEQMKE LIYREALAFN PEYQQ
Length:395
Mass (Da):45,058
Last modified:November 1, 1996 - v1
Checksum:i296D2BD753C6DDA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21842 mRNA. Translation: BAA04869.1.
AC002333 Genomic DNA. Translation: AAB64027.1.
CP002685 Genomic DNA. Translation: AEC10325.1.
AY120737 mRNA. Translation: AAM53295.1.
BT008855 mRNA. Translation: AAP68294.1.
PIRiS40472.
RefSeqiNP_181907.1. NM_129941.4.
UniGeneiAt.22266.
At.53112.

Genome annotation databases

EnsemblPlantsiAT2G43790.1; AT2G43790.1; AT2G43790.
GeneIDi818982.
GrameneiAT2G43790.1; AT2G43790.1; AT2G43790.
KEGGiath:AT2G43790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21842 mRNA. Translation: BAA04869.1.
AC002333 Genomic DNA. Translation: AAB64027.1.
CP002685 Genomic DNA. Translation: AEC10325.1.
AY120737 mRNA. Translation: AAM53295.1.
BT008855 mRNA. Translation: AAP68294.1.
PIRiS40472.
RefSeqiNP_181907.1. NM_129941.4.
UniGeneiAt.22266.
At.53112.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CI6X-ray3.00A/B29-395[»]
ProteinModelPortaliQ39026.
SMRiQ39026.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4318. 32 interactors.
DIPiDIP-31825N.
IntActiQ39026. 12 interactors.
MINTiMINT-8086250.
STRINGi3702.AT2G43790.1.

PTM databases

iPTMnetiQ39026.

Proteomic databases

PaxDbiQ39026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43790.1; AT2G43790.1; AT2G43790.
GeneIDi818982.
GrameneiAT2G43790.1; AT2G43790.1; AT2G43790.
KEGGiath:AT2G43790.

Organism-specific databases

TAIRiAT2G43790.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39026.
KOiK14512.
OMAiMDGGAQP.
OrthoDBiEOG09360BWL.
PhylomeDBiQ39026.

Enzyme and pathway databases

BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ39026.

Gene expression databases

GenevisibleiQ39026. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK6_ARATH
AccessioniPrimary (citable) accession number: Q39026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.