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Protein

Mitogen-activated protein kinase 5

Gene

MPK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated by the MAP kinase kinase MKK2. Activated by the MAP kinase kinase MKK6 in vitro.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721ATPPROSITE-ProRule annotation
Active sitei169 – 1691Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 579ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G11330-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiREACT_273308. ERK/MAPK targets.
REACT_274021. ERK2 activation.
REACT_290617. Signaling by FGFR.
REACT_310320. CREB phosphorylation through the activation of Ras.
REACT_310958. ERKs are inactivated.
REACT_322522. Signalling to ERK5.
REACT_330337. Signal transduction by L1.
REACT_340308. ERK1 activation.
REACT_340626. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 5 (EC:2.7.11.24)
Short name:
AtMPK5
Short name:
MAP kinase 5
Gene namesi
Name:MPK5
Ordered Locus Names:At4g11330
ORF Names:F8L21.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G11330.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Mitogen-activated protein kinase 5PRO_0000186314Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011PhosphothreonineBy similarity
Modified residuei203 – 2031PhosphotyrosineBy similarity
Modified residuei206 – 2061PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylated on threonine and tyrosine residues.By similarity
Dually phosphorylated on Thr-201 and Tyr-203, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39025.
PRIDEiQ39025.

Expressioni

Gene expression databases

ExpressionAtlasiQ39025. baseline and differential.
GenevestigatoriQ39025.

Interactioni

Protein-protein interaction databases

IntActiQ39025. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ39025.
SMRiQ39025. Positions 49-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 329287Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 2033TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ39025.
KOiK04371.
OMAiSETHEEI.
PhylomeDBiQ39025.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEIESATD LGDTNIKGVL VHGGRYFQYN VYGNLFEVSN KYVPPIRPIG
60 70 80 90 100
RGAYGFVCAA VDSETHEEIA IKKIGKAFDN KVDAKRTLRE IKLLRHLEHE
110 120 130 140 150
NVVVIKDIIR PPKKEDFVDV YIVFELMDTD LHQIIRSNQS LNDDHCQYFL
160 170 180 190 200
YQILRGLKYI HSANVLHRDL KPSNLLLNSN CDLKITDFGL ARTTSETEYM
210 220 230 240 250
TEYVVTRWYR APELLLNSSE YTSAIDVWSV GCIFAEIMTR EPLFPGKDYV
260 270 280 290 300
HQLKLITELI GSPDGASLEF LRSANARKYV KELPKFPRQN FSARFPSMNS
310 320 330 340 350
TAIDLLEKML VFDPVKRITV EEALCYPYLS ALHDLNDEPV CSNHFSFHFE
360 370
DPSSTEEEIK ELVWLESVKF NPLPSI
Length:376
Mass (Da):43,207
Last modified:June 5, 2002 - v2
Checksum:i2E8C0E5FC47685DF
GO

Sequence cautioni

The sequence CAB51417.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB81234.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591A → P in BAA04868 (PubMed:8282107).Curated
Sequence conflicti276 – 2772AR → GG in BAA04868 (PubMed:8282107).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21841 mRNA. Translation: BAA04868.1.
AL096882 Genomic DNA. Translation: CAB51417.1. Sequence problems.
AL161531 Genomic DNA. Translation: CAB81234.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82997.1.
AK176361 mRNA. Translation: BAD44124.1.
PIRiS40471.
T13024.
RefSeqiNP_567378.4. NM_117204.6.
UniGeneiAt.264.

Genome annotation databases

EnsemblPlantsiAT4G11330.1; AT4G11330.1; AT4G11330.
GeneIDi826735.
KEGGiath:AT4G11330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21841 mRNA. Translation: BAA04868.1.
AL096882 Genomic DNA. Translation: CAB51417.1. Sequence problems.
AL161531 Genomic DNA. Translation: CAB81234.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82997.1.
AK176361 mRNA. Translation: BAD44124.1.
PIRiS40471.
T13024.
RefSeqiNP_567378.4. NM_117204.6.
UniGeneiAt.264.

3D structure databases

ProteinModelPortaliQ39025.
SMRiQ39025. Positions 49-366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ39025. 1 interaction.

Proteomic databases

PaxDbiQ39025.
PRIDEiQ39025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G11330.1; AT4G11330.1; AT4G11330.
GeneIDi826735.
KEGGiath:AT4G11330.

Organism-specific databases

GeneFarmi826. 89.
TAIRiAT4G11330.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ39025.
KOiK04371.
OMAiSETHEEI.
PhylomeDBiQ39025.

Enzyme and pathway databases

BioCyciARA:AT4G11330-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiREACT_273308. ERK/MAPK targets.
REACT_274021. ERK2 activation.
REACT_290617. Signaling by FGFR.
REACT_310320. CREB phosphorylation through the activation of Ras.
REACT_310958. ERKs are inactivated.
REACT_322522. Signalling to ERK5.
REACT_330337. Signal transduction by L1.
REACT_340308. ERK1 activation.
REACT_340626. Regulation of HSF1-mediated heat shock response.

Gene expression databases

ExpressionAtlasiQ39025. baseline and differential.
GenevestigatoriQ39025.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana."
    Mizoguchi T., Hayashida N., Yamaguchi-Shinozaki K., Kamada H., Shinozaki K.
    FEBS Lett. 336:440-444(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: ENZYME REGULATION.
  7. Cited for: GENE FAMILY.
  8. "The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana."
    Kosetsu K., Matsunaga S., Nakagami H., Colcombet J., Sasabe M., Soyano T., Takahashi Y., Hirt H., Machida Y.
    Plant Cell 22:3778-3790(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.

Entry informationi

Entry nameiMPK5_ARATH
AccessioniPrimary (citable) accession number: Q39025
Secondary accession number(s): Q67YV7, Q9SUS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: June 5, 2002
Last modified: March 31, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.