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Protein

Mitogen-activated protein kinase 4

Gene

MPK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The ANPs-MKK6-MPK4 module is involved in the regulation of plant cytokinesis during meiosis and mitosis. Essential to promote the progression of cytokinesis and for cellularization (formation of the cell plate) during male-specific meiosis. Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1. Involved in root hair development process. Negative regulator of systemic acquired resistance (SAR) and salicylic acid- (SA) mediated defense response. Required for jasmonic acid- (JA) mediated defense gene expression. May regulate activity of transcription factor controlling pathogenesis-related (PR) gene expression. Seems to act independently of the SAR regulatory protein NPR1 (Nonexpresser of PR1). Phosphorylates MKS1 and transcription factors WRKY25 and WRKY33. The MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity (PubMed:11163186, PubMed:15225555, PubMed:15358537, PubMed:15990873, PubMed:18982020, PubMed:20215588, PubMed:20802223, PubMed:21098735, PubMed:21575092, PubMed:25770109). Phosphorylates MEKK2 upon treatment with flg22 (PubMed:22643122).11 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Activated by the MAP kinase kinases MKK1 and MKK2. Activated in response to touch, wounding, low temperature, low humidity, salt stress and the bacterial elicitors flagellin and harpin. Activated upon Pseudomonas syringae pv. tomato DC3000 infection. Repressed by the protein phosphatase 2C AP2C1. Repressed by DSPTP1-mediated dephosphorylation. Activated by the MAP kinase kinase MKK6 in vitro.12 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72ATPPROSITE-ProRule annotation1
Active sitei169Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 57ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB
  • MAP kinase activity Source: TAIR
  • protein kinase activity Source: UniProtKB

GO - Biological processi

  • cortical microtubule organization Source: UniProtKB
  • cytokinesis by cell plate formation Source: UniProtKB
  • hyperosmotic response Source: TAIR
  • hypotonic salinity response Source: TAIR
  • jasmonic acid and ethylene-dependent systemic resistance Source: TAIR
  • jasmonic acid and ethylene-dependent systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • male meiosis cytokinesis Source: TAIR
  • phosphorylation Source: TAIR
  • pollen development Source: TAIR
  • protein phosphorylation Source: UniProtKB
  • regulation of gene expression Source: GO_Central
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to fungus Source: TAIR
  • response to salt stress Source: TAIR
  • systemic acquired resistance, salicylic acid mediated signaling pathway Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processImmunity, Innate immunity, Plant defense, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 399
ReactomeiR-ATH-198753 ERK/MAPK targets
R-ATH-198765 Signalling to ERK5
R-ATH-202670 ERKs are inactivated
R-ATH-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-ATH-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 4 (EC:2.7.11.24)
Short name:
AtMPK4
Short name:
MAP kinase 4
Gene namesi
Name:MPK4
Ordered Locus Names:At4g01370
ORF Names:F2N1.1, F2N1_2-t
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G01370
TAIRilocus:2124943 AT4G01370

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Disruption phenotypei

Dwarf plants with cytokinetic defects in leaf epidermal cells. Abnormally developed and branched root hairs. Retarded root growth with the protrusion of many epidermal cells from roots. Heavily bundled and disoriented cortical microtubules resistant to oryzalin. Exhibits a seedling-lethality phenotype. Defects in the formation of the cell plate. Abnormal mature pollen grains.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187D → A: Loss of kinase activity. 1 Publication1
Mutagenesisi201T → A: Loss of kinase activity; when associated with Y-203. 1 Publication1
Mutagenesisi203Y → F: Loss of kinase activity; when associated with T-201. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863131 – 376Mitogen-activated protein kinase 4Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201Phosphothreonine1 Publication1
Modified residuei203Phosphotyrosine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-201 and Tyr-203, which activates the enzyme. Autophosphorylated on serine and tyrosine residues. Dephosphorylated by DSPTP1. Phosphorylated by MKK6 in vitro.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39024
PRIDEiQ39024

PTM databases

iPTMnetiQ39024

Expressioni

Tissue specificityi

Ubiquitous. Expressed in the veins and stomatal guard cells of leaf plates, petioles, stem, roots and flowers.3 Publications

Developmental stagei

Observed in root epidermal cells and root hairs, especially in the root hair formation zone. During root hair development, both observed in root hair bulges and in young outgrowing root hairs.1 Publication

Gene expression databases

ExpressionAtlasiQ39024 baseline and differential
GenevisibleiQ39024 AT

Interactioni

Subunit structurei

Interacts with MEKK1, MKK1, MKK2 and MKK6. May form a ternary complex composed of MEKK1 and MKK1/MKK2 and MPK4. Interacts with MKS1 and AP2C1. May form a ternary or larger complex with MKS1 and WRKY25 and/or WRKY33. Interacts with MAP65-1 (PubMed:15225555, PubMed:15990873, PubMed:17630279, PubMed:18982020, PubMed:19513235, PubMed:20215588, PubMed:20802223, PubMed:21575092, PubMed:9878570). No interactions with RACK1A, RACK1B or RACK1C (PubMed:25731164). Interacts directly with ASR3 and mediates its phosphorylation (PubMed:25770109). Binds to MEKK2 (PubMed:22643122). Interacts with PAT1 (PubMed:25603932).13 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi13440, 14 interactors
IntActiQ39024, 7 interactors
MINTiQ39024
STRINGi3702.AT4G01370.1

Structurei

3D structure databases

ProteinModelPortaliQ39024
SMRiQ39024
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 329Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 203TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
InParanoidiQ39024
KOiK20600
OMAiTVCSAWD
OrthoDBiEOG09360BXQ
PhylomeDBiQ39024

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q39024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAESCFGSS GDQSSSKGVA THGGSYVQYN VYGNLFEVSR KYVPPLRPIG
60 70 80 90 100
RGAYGIVCAA TNSETGEEVA IKKIGNAFDN IIDAKRTLRE IKLLKHMDHE
110 120 130 140 150
NVIAVKDIIK PPQRENFNDV YIVYELMDTD LHQIIRSNQP LTDDHCRFFL
160 170 180 190 200
YQLLRGLKYV HSANVLHRDL KPSNLLLNAN CDLKLGDFGL ARTKSETDFM
210 220 230 240 250
TEYVVTRWYR APELLLNCSE YTAAIDIWSV GCILGETMTR EPLFPGKDYV
260 270 280 290 300
HQLRLITELI GSPDDSSLGF LRSDNARRYV RQLPQYPRQN FAARFPNMSA
310 320 330 340 350
GAVDLLEKML VFDPSRRITV DEALCHPYLA PLHDINEEPV CVRPFNFDFE
360 370
QPTLTEENIK ELIYRETVKF NPQDSV
Length:376
Mass (Da):42,852
Last modified:June 6, 2002 - v2
Checksum:i448F65C25C95AAEB
GO

Sequence cautioni

The sequence AAB61033 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313D → E in BAA04867 (PubMed:8282107).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti115E → Q in strain: cv. C24, cv. Lz-0, cv. Wei-0, cv. Yo-0. 1 Publication1
Natural varianti293A → V in strain: cv. Ak-1, cv. Bay-0, cv. Di-0, cv. Landsberg erecta, cv. Tsu-0, cv. Wei-0. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21840 mRNA Translation: BAA04867.1
EF470667 Genomic DNA Translation: ABR46145.1
EF470668 Genomic DNA Translation: ABR46146.1
EF470669 Genomic DNA Translation: ABR46147.1
EF470670 Genomic DNA Translation: ABR46148.1
EF470671 Genomic DNA Translation: ABR46149.1
EF470672 Genomic DNA Translation: ABR46150.1
EF470673 Genomic DNA Translation: ABR46151.1
EF470674 Genomic DNA Translation: ABR46152.1
EF470675 Genomic DNA Translation: ABR46153.1
EF470676 Genomic DNA Translation: ABR46154.1
EF470677 Genomic DNA Translation: ABR46155.1
EF470678 Genomic DNA Translation: ABR46156.1
EF470679 Genomic DNA Translation: ABR46157.1
EF470680 Genomic DNA Translation: ABR46158.1
EF470681 Genomic DNA Translation: ABR46159.1
EF470682 Genomic DNA Translation: ABR46160.1
EF470683 Genomic DNA Translation: ABR46161.1
EF470684 Genomic DNA Translation: ABR46162.1
EF470685 Genomic DNA Translation: ABR46163.1
EF470686 Genomic DNA Translation: ABR46164.1
DQ112072 Genomic DNA Translation: AAZ20637.1
AF007269 Genomic DNA Translation: AAB61033.1 Sequence problems.
AL161491 Genomic DNA Translation: CAB80946.1
CP002687 Genomic DNA Translation: AEE82016.1
AF360231 mRNA Translation: AAK25941.1
AY040031 mRNA Translation: AAK64089.1
AY088537 mRNA Translation: AAM66070.1
PIRiS40470
RefSeqiNP_192046.1, NM_116367.3
UniGeneiAt.19915

Genome annotation databases

EnsemblPlantsiAT4G01370.1; AT4G01370.1; AT4G01370
GeneIDi828151
GrameneiAT4G01370.1; AT4G01370.1; AT4G01370
KEGGiath:AT4G01370

Similar proteinsi

Entry informationi

Entry nameiMPK4_ARATH
AccessioniPrimary (citable) accession number: Q39024
Secondary accession number(s): B2BDE5
, B2BDE6, B2BDE8, B2BDG2, O04597, Q45V20, Q9M136
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: May 23, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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