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Protein

Mitogen-activated protein kinase 4

Gene

MPK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The ANPs-MKK6-MPK4 module is involved in the regulation of plant cytokinesis during meiosis and mitosis. Essential to promote the progression of cytokinesis and for cellularization (formation of the cell plate) during male-specific meiotic. Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1. Involved in root hair development process. Negative regulator of systemic acquired resistance (SAR) and salicylic acid- (SA) mediated defense response. Required for jasmonic acid- (JA) mediated defense gene expression. May regulate activity of transcription factor controlling pathogenesis-related (PR) gene expression. Seems to act independently of the SAR regulatory protein NPR1 (Nonexpresser of PR1). Phosphorylates MKS1 and transcription factors WRKY25 and WRKY33. The MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity (PubMed:11163186, PubMed:15225555, PubMed:15358537, PubMed:15990873, PubMed:18982020, PubMed:20215588, PubMed:20802223, PubMed:21098735, PubMed:21575092, PubMed:25770109). Phosphorylates MEKK2 upon treatment with flg22 (PubMed:22643122).11 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Activated by the MAP kinase kinases MKK1 and MKK2. Activated in response to touch, wounding, low temperature, low humidity, salt stress and the bacterial elicitors flagellin and harpin. Activated upon Pseudomonas syringae pv. tomato DC3000 infection. Repressed by the protein phosphatase 2C AP2C1. Repressed by DSPTP1-mediated dephosphorylation. Activated by the MAP kinase kinase MKK6 in vitro.12 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72ATPPROSITE-ProRule annotation1
Active sitei169Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 57ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB
  • MAP kinase activity Source: TAIR
  • protein kinase activity Source: UniProtKB

GO - Biological processi

  • cortical microtubule organization Source: UniProtKB
  • cytokinesis by cell plate formation Source: UniProtKB
  • hyperosmotic response Source: TAIR
  • hypotonic salinity response Source: TAIR
  • jasmonic acid and ethylene-dependent systemic resistance Source: TAIR
  • jasmonic acid and ethylene-dependent systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • male meiosis cytokinesis Source: TAIR
  • phosphorylation Source: TAIR
  • pollen development Source: TAIR
  • protein phosphorylation Source: UniProtKB
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to fungus Source: TAIR
  • response to salt stress Source: TAIR
  • systemic acquired resistance, salicylic acid mediated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Immunity, Innate immunity, Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 4 (EC:2.7.11.24)
Short name:
AtMPK4
Short name:
MAP kinase 4
Gene namesi
Name:MPK4
Ordered Locus Names:At4g01370
ORF Names:F2N1.1, F2N1_2-t
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G01370.

Subcellular locationi

GO - Cellular componenti

  • cell plate Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • microtubule Source: UniProtKB-KW
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Disruption phenotypei

Dwarf plants with cytokinetic defects in leaf epidermal cells. Abnormally developed and branched root hairs. Retarded root growth with the protrusion of many epidermal cells from roots. Heavily bundled and disoriented cortical microtubules resistant to oryzalin. Exhibits a seedling-lethality phenotype. Defects in the formation of the cell plate. Abnormal mature pollen grains.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187D → A: Loss of kinase activity. 1 Publication1
Mutagenesisi201T → A: Loss of kinase activity; when associated with Y-203. 1 Publication1
Mutagenesisi203Y → F: Loss of kinase activity; when associated with T-201. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863131 – 376Mitogen-activated protein kinase 4Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201Phosphothreonine1 Publication1
Modified residuei203Phosphotyrosine1 Publication1
Modified residuei206PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-201 and Tyr-203, which activates the enzyme. Autophosphorylated on serine and tyrosine residues. Dephosphorylated by DSPTP1. Phosphorylated by MKK6 in vitro.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39024.
PRIDEiQ39024.

PTM databases

iPTMnetiQ39024.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in the veins and stomatal guard cells of leaf plates, petioles, stem, roots and flowers.3 Publications

Developmental stagei

Observed in root epidermal cells and root hairs, especially in the root hair formation zone. During root hair development, both observed in root hair bulges and in young outgrowing root hairs.1 Publication

Gene expression databases

GenevisibleiQ39024. AT.

Interactioni

Subunit structurei

Interacts with MEKK1, MKK1, MKK2 and MKK6. May form a ternary complex composed of MEKK1 and MKK1/MKK2 and MPK4. Interacts with MKS1 and AP2C1. May form a ternary or larger complex with MKS1 and WRKY25 and/or WRKY33. Interacts with MAP65-1 (PubMed:15225555, PubMed:15990873, PubMed:17630279, PubMed:18982020, PubMed:19513235, PubMed:20215588, PubMed:20802223, PubMed:21575092, PubMed:9878570). No interactions with RACK1A, RACK1B or RACK1C (PubMed:25731164). Interacts directly with ASR3 and mediates its phosphorylation (PubMed:25770109). Binds to MEKK2 (PubMed:22643122).12 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MEKK2O814723EBI-994375,EBI-6271434
MKK1Q94A066EBI-994375,EBI-994464
MKK2Q9S7U95EBI-994375,EBI-994350
MKS1Q8LGD59EBI-994375,EBI-1392198
WRKY25O229212EBI-994375,EBI-1392386
WRKY33Q8S8P52EBI-994375,EBI-1392374

Protein-protein interaction databases

BioGridi13440. 13 interactors.
IntActiQ39024. 7 interactors.
MINTiMINT-7232331.
STRINGi3702.AT4G01370.1.

Structurei

3D structure databases

ProteinModelPortaliQ39024.
SMRiQ39024.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 329Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 203TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
InParanoidiQ39024.
KOiK20600.
OMAiIKGMATH.
OrthoDBiEOG09360BXQ.
PhylomeDBiQ39024.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAESCFGSS GDQSSSKGVA THGGSYVQYN VYGNLFEVSR KYVPPLRPIG
60 70 80 90 100
RGAYGIVCAA TNSETGEEVA IKKIGNAFDN IIDAKRTLRE IKLLKHMDHE
110 120 130 140 150
NVIAVKDIIK PPQRENFNDV YIVYELMDTD LHQIIRSNQP LTDDHCRFFL
160 170 180 190 200
YQLLRGLKYV HSANVLHRDL KPSNLLLNAN CDLKLGDFGL ARTKSETDFM
210 220 230 240 250
TEYVVTRWYR APELLLNCSE YTAAIDIWSV GCILGETMTR EPLFPGKDYV
260 270 280 290 300
HQLRLITELI GSPDDSSLGF LRSDNARRYV RQLPQYPRQN FAARFPNMSA
310 320 330 340 350
GAVDLLEKML VFDPSRRITV DEALCHPYLA PLHDINEEPV CVRPFNFDFE
360 370
QPTLTEENIK ELIYRETVKF NPQDSV
Length:376
Mass (Da):42,852
Last modified:June 6, 2002 - v2
Checksum:i448F65C25C95AAEB
GO

Sequence cautioni

The sequence AAB61033 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313D → E in BAA04867 (PubMed:8282107).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti115E → Q in strain: cv. C24, cv. Lz-0, cv. Wei-0, cv. Yo-0. 1 Publication1
Natural varianti293A → V in strain: cv. Ak-1, cv. Bay-0, cv. Di-0, cv. Landsberg erecta, cv. Tsu-0, cv. Wei-0. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21840 mRNA. Translation: BAA04867.1.
EF470667 Genomic DNA. Translation: ABR46145.1.
EF470668 Genomic DNA. Translation: ABR46146.1.
EF470669 Genomic DNA. Translation: ABR46147.1.
EF470670 Genomic DNA. Translation: ABR46148.1.
EF470671 Genomic DNA. Translation: ABR46149.1.
EF470672 Genomic DNA. Translation: ABR46150.1.
EF470673 Genomic DNA. Translation: ABR46151.1.
EF470674 Genomic DNA. Translation: ABR46152.1.
EF470675 Genomic DNA. Translation: ABR46153.1.
EF470676 Genomic DNA. Translation: ABR46154.1.
EF470677 Genomic DNA. Translation: ABR46155.1.
EF470678 Genomic DNA. Translation: ABR46156.1.
EF470679 Genomic DNA. Translation: ABR46157.1.
EF470680 Genomic DNA. Translation: ABR46158.1.
EF470681 Genomic DNA. Translation: ABR46159.1.
EF470682 Genomic DNA. Translation: ABR46160.1.
EF470683 Genomic DNA. Translation: ABR46161.1.
EF470684 Genomic DNA. Translation: ABR46162.1.
EF470685 Genomic DNA. Translation: ABR46163.1.
EF470686 Genomic DNA. Translation: ABR46164.1.
DQ112072 Genomic DNA. Translation: AAZ20637.1.
AF007269 Genomic DNA. Translation: AAB61033.1. Sequence problems.
AL161491 Genomic DNA. Translation: CAB80946.1.
CP002687 Genomic DNA. Translation: AEE82016.1.
AF360231 mRNA. Translation: AAK25941.1.
AY040031 mRNA. Translation: AAK64089.1.
AY088537 mRNA. Translation: AAM66070.1.
PIRiS40470.
RefSeqiNP_192046.1. NM_116367.3.
UniGeneiAt.19915.

Genome annotation databases

EnsemblPlantsiAT4G01370.1; AT4G01370.1; AT4G01370.
GeneIDi828151.
GrameneiAT4G01370.1; AT4G01370.1; AT4G01370.
KEGGiath:AT4G01370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21840 mRNA. Translation: BAA04867.1.
EF470667 Genomic DNA. Translation: ABR46145.1.
EF470668 Genomic DNA. Translation: ABR46146.1.
EF470669 Genomic DNA. Translation: ABR46147.1.
EF470670 Genomic DNA. Translation: ABR46148.1.
EF470671 Genomic DNA. Translation: ABR46149.1.
EF470672 Genomic DNA. Translation: ABR46150.1.
EF470673 Genomic DNA. Translation: ABR46151.1.
EF470674 Genomic DNA. Translation: ABR46152.1.
EF470675 Genomic DNA. Translation: ABR46153.1.
EF470676 Genomic DNA. Translation: ABR46154.1.
EF470677 Genomic DNA. Translation: ABR46155.1.
EF470678 Genomic DNA. Translation: ABR46156.1.
EF470679 Genomic DNA. Translation: ABR46157.1.
EF470680 Genomic DNA. Translation: ABR46158.1.
EF470681 Genomic DNA. Translation: ABR46159.1.
EF470682 Genomic DNA. Translation: ABR46160.1.
EF470683 Genomic DNA. Translation: ABR46161.1.
EF470684 Genomic DNA. Translation: ABR46162.1.
EF470685 Genomic DNA. Translation: ABR46163.1.
EF470686 Genomic DNA. Translation: ABR46164.1.
DQ112072 Genomic DNA. Translation: AAZ20637.1.
AF007269 Genomic DNA. Translation: AAB61033.1. Sequence problems.
AL161491 Genomic DNA. Translation: CAB80946.1.
CP002687 Genomic DNA. Translation: AEE82016.1.
AF360231 mRNA. Translation: AAK25941.1.
AY040031 mRNA. Translation: AAK64089.1.
AY088537 mRNA. Translation: AAM66070.1.
PIRiS40470.
RefSeqiNP_192046.1. NM_116367.3.
UniGeneiAt.19915.

3D structure databases

ProteinModelPortaliQ39024.
SMRiQ39024.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13440. 13 interactors.
IntActiQ39024. 7 interactors.
MINTiMINT-7232331.
STRINGi3702.AT4G01370.1.

PTM databases

iPTMnetiQ39024.

Proteomic databases

PaxDbiQ39024.
PRIDEiQ39024.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G01370.1; AT4G01370.1; AT4G01370.
GeneIDi828151.
GrameneiAT4G01370.1; AT4G01370.1; AT4G01370.
KEGGiath:AT4G01370.

Organism-specific databases

TAIRiAT4G01370.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
InParanoidiQ39024.
KOiK20600.
OMAiIKGMATH.
OrthoDBiEOG09360BXQ.
PhylomeDBiQ39024.

Enzyme and pathway databases

BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ39024.

Gene expression databases

GenevisibleiQ39024. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK4_ARATH
AccessioniPrimary (citable) accession number: Q39024
Secondary accession number(s): B2BDE5
, B2BDE6, B2BDE8, B2BDG2, O04597, Q45V20, Q9M136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: November 30, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.