Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q39023 (MPK3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 3

Short name=AtMPK3
Short name=MAP kinase 3
EC=2.7.11.24
Gene names
Name:MPK3
Ordered Locus Names:At3g45640
ORF Names:F9K21.220, T6D9.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade. May phosphorylate regulators of WRKY transcription factors. Mediates the phosphorylation of VIP1 and subsequent stress genes transcription in response to Agrobacterium. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs. Ref.9 Ref.13 Ref.16 Ref.17 Ref.18 Ref.19 Ref.21 Ref.22

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation. Activated by MAP kinase kinases MKK4, MKK5, MKK7 and MKK9. Activated in response to hydrogen peroxide, ozone, salt stress and flagellin bacterial elicitor. Triggered by Agrobacterium upon T-DNA transfer. Repressed by DSPTP1B/MKP2-mediated dephosphorylation. Ref.6 Ref.7 Ref.9 Ref.12 Ref.15 Ref.17 Ref.18 Ref.21

Subunit structure

Interacts with DSPTP1B/MKP2, NDPK2 and VIP1. The interaction with DSPTP1B/MKP2 is repressed by fungal elicitation. Ref.11 Ref.17 Ref.20

Subcellular location

Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation Probable. Ref.12

Induction

By touch, cold, salinity stress and ozone. Ref.5 Ref.6 Ref.7 Ref.9 Ref.12 Ref.15 Ref.17 Ref.18 Ref.21

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-196 and Tyr-198, which activates the enzyme By similarity. Dephosphorylated by DSPTP1B/MKP2. Ref.8 Ref.15

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processHypersensitive response
Plant defense
Stress response
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processabscisic acid-activated signaling pathway

Traceable author statement PubMed 12434021. Source: TAIR

activation of MAPK activity involved in osmosensory signaling pathway

Inferred from direct assay Ref.7. Source: TAIR

camalexin biosynthetic process

Inferred from mutant phenotype Ref.19. Source: TAIR

inflorescence development

Inferred from genetic interaction Ref.22. Source: TAIR

ovule development

Inferred from genetic interaction PubMed 18364464. Source: TAIR

plant-type hypersensitive response

Inferred from electronic annotation. Source: UniProtKB-KW

priming of cellular response to stress

Inferred from mutant phenotype PubMed 19318610. Source: TAIR

regulation of stomatal complex development

Inferred from genetic interaction Ref.16. Source: TAIR

regulation of stomatal complex patterning

Inferred from genetic interaction Ref.16. Source: TAIR

response to UV-B

Inferred from mutant phenotype PubMed 21790814. Source: TAIR

response to bacterium

Inferred from expression pattern PubMed 19318610. Source: TAIR

response to chitin

Inferred from expression pattern PubMed 15923325. Source: TAIR

response to cold

Inferred from expression pattern Ref.5. Source: TAIR

response to osmotic stress

Inferred from direct assay Ref.7. Source: TAIR

response to oxidative stress

Inferred from expression pattern Ref.6. Source: TAIR

response to wounding

Inferred from expression pattern PubMed 17953483. Source: TAIR

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein binding

Inferred from physical interaction Ref.17Ref.20. Source: UniProtKB

protein kinase activity

Inferred from direct assay PubMed 22631074. Source: TAIR

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

At2g47060O807192EBI-349526,EBI-4436376
NDPK2O649034EBI-349526,EBI-349517

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Mitogen-activated protein kinase 3
PRO_0000186312

Regions

Domain38 – 324287Protein kinase
Nucleotide binding44 – 529ATP By similarity
Motif196 – 1983TXY

Sites

Active site1641Proton acceptor By similarity
Binding site671ATP By similarity

Amino acid modifications

Modified residue1961Phosphothreonine By similarity
Modified residue1981Phosphotyrosine By similarity
Modified residue2011Phosphothreonine By similarity

Experimental info

Sequence conflict151E → D in BAA04866. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q39023 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: 6992A7D97F3C9841

FASTA37042,717
        10         20         30         40         50         60 
MNTGGGQYTD FPAVETHGGQ FISYDIFGSL FEITSKYRPP IIPIGRGAYG IVCSVLDTET 

        70         80         90        100        110        120 
NELVAMKKIA NAFDNHMDAK RTLREIKLLR HLDHENIIAI RDVVPPPLRR QFSDVYISTE 

       130        140        150        160        170        180 
LMDTDLHQII RSNQSLSEEH CQYFLYQLLR GLKYIHSANI IHRDLKPSNL LLNANCDLKI 

       190        200        210        220        230        240 
CDFGLARPTS ENDFMTEYVV TRWYRAPELL LNSSDYTAAI DVWSVGCIFM ELMNRKPLFP 

       250        260        270        280        290        300 
GKDHVHQMRL LTELLGTPTE SDLGFTHNED AKRYIRQLPN FPRQPLAKLF SHVNPMAIDL 

       310        320        330        340        350        360 
VDRMLTFDPN RRITVEQALN HQYLAKLHDP NDEPICQKPF SFEFEQQPLD EEQIKEMIYQ 

       370 
EAIALNPTYG 

« Hide

References

« Hide 'large scale' references
[1]"ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana."
Mizoguchi T., Hayashida N., Yamaguchi-Shinozaki K., Kamada H., Shinozaki K.
FEBS Lett. 336:440-444(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana."
Mizoguchi T., Irie K., Hirayama T., Hayashida N., Yamaguchi-Shinozaki K., Matsumoto K., Shinozaki K.
Proc. Natl. Acad. Sci. U.S.A. 93:765-769(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants."
Kovtun Y., Chiu W.-L., Tena G., Sheen J.
Proc. Natl. Acad. Sci. U.S.A. 97:2940-2945(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[7]"Different protein kinase families are activated by osmotic stresses in Arabidopsis thaliana cell suspensions. Involvement of the MAP kinases AtMPK3 and AtMPK6."
Droillard M.-J., Boudsocq M., Barbier-Brygoo H., Lauriere C.
FEBS Lett. 527:43-50(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[8]"Activation of AtMEK1, an Arabidopsis mitogen-activated protein kinase kinase, in vitro and in vivo: analysis of active mutants expressed in E. coli and generation of the active form in stress response in seedlings."
Matsuoka D., Nanmori T., Sato K., Fukami Y., Kikkawa U., Yasuda T.
Plant J. 29:637-647(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[9]"MAP kinase signalling cascade in Arabidopsis innate immunity."
Asai T., Tena G., Plotnikova J., Willmann M.R., Chiu W.-L., Gomez-Gomez L., Boller T., Ausubel F.M., Sheen J.
Nature 415:977-983(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[10]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[11]"NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants."
Moon H., Lee B., Choi G., Shin D., Prasad D.T., Lee O., Kwak S.-S., Kim D.H., Nam J., Bahk J., Hong J.C., Lee S.Y., Cho M.J., Lim C.O., Yun D.-J.
Proc. Natl. Acad. Sci. U.S.A. 100:358-363(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NDPK2.
[12]"Stress hormone-independent activation and nuclear translocation of mitogen-activated protein kinases in Arabidopsis thaliana during ozone exposure."
Ahlfors R., Macioszek V., Rudd J., Brosche M., Schlichting R., Scheel D., Kangasjarvi J.
Plant J. 40:512-522(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, SUBCELLULAR LOCATION, INDUCTION.
[13]"RNA interference-based (RNAi) suppression of AtMPK6, an Arabidopsis mitogen-activated protein kinase, results in hypersensitivity to ozone and misregulation of AtMPK3."
Miles G.P., Samuel M.A., Zhang Y., Ellis B.E.
Environ. Pollut. 138:230-237(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[15]"Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs."
Lee J.S., Ellis B.E.
J. Biol. Chem. 282:25020-25029(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, DEPHOSPHORYLATION.
[16]"Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis."
Wang H., Ngwenyama N., Liu Y., Walker J.C., Zhang S.
Plant Cell 19:63-73(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"Trojan horse strategy in Agrobacterium transformation: abusing MAPK defense signaling."
Djamei A., Pitzschke A., Nakagami H., Rajh I., Hirt H.
Science 318:453-456(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH VIP1.
[18]"Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling."
Yoo S.D., Cho Y.H., Tena G., Xiong Y., Sheen J.
Nature 451:789-795(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[19]"A fungal-responsive MAPK cascade regulates phytoalexin biosynthesis in Arabidopsis."
Ren D., Liu Y., Yang K.Y., Han L., Mao G., Glazebrook J., Zhang S.
Proc. Natl. Acad. Sci. U.S.A. 105:5638-5643(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[20]"MAPK phosphatase MKP2 mediates disease responses in Arabidopsis and functionally interacts with MPK3 and MPK6."
Lumbreras V., Vilela B., Irar S., Sole M., Capellades M., Valls M., Coca M., Pages M.
Plant J. 63:1017-1030(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DSPTP1B/MKP2.
[21]"Arabidopsis MKK4 mediates osmotic-stress response via its regulation of MPK3 activity."
Kim S.H., Woo D.H., Kim J.M., Lee S.Y., Chung W.S., Moon Y.H.
Biochem. Biophys. Res. Commun. 412:150-154(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[22]"A MAPK cascade downstream of ERECTA receptor-like protein kinase regulates Arabidopsis inflorescence architecture by promoting localized cell proliferation."
Meng X., Wang H., He Y., Liu Y., Walker J.C., Torii K.U., Zhang S.
Plant Cell 24:4948-4960(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D21839 mRNA. Translation: BAA04866.1.
AL138657 Genomic DNA. Translation: CAB75493.1.
AL157735 Genomic DNA. No translation available.
CP002686 Genomic DNA. Translation: AEE78054.1.
AF386961 mRNA. Translation: AAK62406.1.
BT000007 mRNA. Translation: AAN15326.1.
PIRS40469.
T47504.
RefSeqNP_190150.1. NM_114433.2.
UniGeneAt.263.

3D structure databases

ProteinModelPortalQ39023.
SMRQ39023. Positions 31-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid9026. 29 interactions.
DIPDIP-768N.
IntActQ39023. 18 interactions.
MINTMINT-8086190.

Proteomic databases

PaxDbQ39023.
PRIDEQ39023.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G45640.1; AT3G45640.1; AT3G45640.
GeneID823706.
KEGGath:AT3G45640.

Organism-specific databases

GeneFarm828. 89.
TAIRAT3G45640.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ39023.
KOK04371.
OMAEAYHDPQ.
PhylomeDBQ39023.

Enzyme and pathway databases

BioCycARA:AT3G45640-MONOMER.
BRENDA2.7.11.24. 399.

Gene expression databases

ArrayExpressQ39023.
GenevestigatorQ39023.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ39023.

Entry information

Entry nameMPK3_ARATH
AccessionPrimary (citable) accession number: Q39023
Secondary accession number(s): Q9M1E3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: June 11, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names