UniProtKB - Q39023 (MPK3_ARATH)
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Protein
Mitogen-activated protein kinase 3
Gene
MPK3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Mediates the phosphorylation of VIP1 and subsequent stress genes transcription in response to Agrobacterium. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.9 Publications
Catalytic activityi
ATP + a protein = ADP + a phosphoprotein.
Enzyme regulationi
Activated by threonine and tyrosine phosphorylation. Activated by MAP kinase kinases MKK4, MKK5, MKK7 and MKK9. Activated in response to hydrogen peroxide, ozone, salt stress and flagellin bacterial elicitor. Triggered by Agrobacterium upon T-DNA transfer. Repressed by DSPTP1B/MKP2-mediated dephosphorylation.8 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 67 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 164 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 44 – 52 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase activity Source: GO_Central
- protein kinase activity Source: TAIR
GO - Biological processi
- abscisic acid-activated signaling pathway Source: TAIR
- camalexin biosynthetic process Source: TAIR
- defense response to bacterium Source: EnsemblPlants
- defense response to fungus Source: EnsemblPlants
- inflorescence development Source: TAIR
- plant ovule development Source: TAIR
- plant-type hypersensitive response Source: UniProtKB-KW
- pollen development Source: TAIR
- pollen tube guidance Source: TAIR
- positive regulation of response to salt stress Source: EnsemblPlants
- priming of cellular response to stress Source: TAIR
- protein phosphorylation Source: TAIR
- regulation of gene expression Source: GO_Central
- response to bacterium Source: TAIR
- response to chitin Source: TAIR
- response to cold Source: TAIR
- response to freezing Source: TAIR
- response to osmotic stress Source: TAIR
- response to oxidative stress Source: TAIR
- response to UV-B Source: TAIR
- response to water deprivation Source: EnsemblPlants
- response to wounding Source: TAIR
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Hypersensitive response, Plant defense, Stress response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24. 399. |
Reactomei | R-ATH-110056. MAPK3 (ERK1) activation. R-ATH-112409. RAF-independent MAPK1/3 activation. R-ATH-112411. MAPK1 (ERK2) activation. R-ATH-198753. ERK/MAPK targets. R-ATH-198765. Signalling to ERK5. R-ATH-202670. ERKs are inactivated. R-ATH-2559582. Senescence-Associated Secretory Phenotype (SASP). R-ATH-3371453. Regulation of HSF1-mediated heat shock response. R-ATH-375165. NCAM signaling for neurite out-growth. R-ATH-442742. CREB phosphorylation through the activation of Ras. R-ATH-444257. RSK activation. R-ATH-445144. Signal transduction by L1. R-ATH-5673001. RAF/MAP kinase cascade. R-ATH-5674135. MAP2K and MAPK activation. R-ATH-5674499. Negative feedback regulation of MAPK pathway. R-ATH-6798695. Neutrophil degranulation. R-ATH-881907. Gastrin-CREB signalling pathway via PKC and MAPK. |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 3 (EC:2.7.11.24)Short name: AtMPK3 Short name: MAP kinase 3 |
Gene namesi | Name:MPK3 Ordered Locus Names:At3g45640 ORF Names:F9K21.220, T6D9.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G45640. |
TAIRi | locus:2085632. AT3G45640. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186312 | 1 – 370 | Mitogen-activated protein kinase 3Add BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 196 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 198 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 201 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Dually phosphorylated on Thr-196 and Tyr-198, which activates the enzyme (By similarity). Dephosphorylated by DSPTP1B/MKP2.By similarity1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q39023. |
PTM databases
iPTMneti | Q39023. |
Expressioni
Inductioni
By touch, cold, salinity stress and ozone.2 Publications
Gene expression databases
ExpressionAtlasi | Q39023. baseline and differential. |
Genevisiblei | Q39023. AT. |
Interactioni
Subunit structurei
Interacts with DSPTP1B/MKP2, NDPK2 and VIP1. The interaction with DSPTP1B/MKP2 is repressed by fungal elicitation (PubMed:12506203, PubMed:17947581, PubMed:20626661). Binds to LIP5 (PubMed:25010425). Interacts with VQ4 (PubMed:24750137). Interacts with RACK1A, RACK1B and RACK1C (PubMed:25731164).6 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 9026. 39 interactors. |
DIPi | DIP-768N. |
ELMi | Q39023. |
IntActi | Q39023. 18 interactors. |
MINTi | Q39023. |
STRINGi | 3702.AT3G45640.1. |
Structurei
3D structure databases
ProteinModelPortali | Q39023. |
SMRi | Q39023. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 324 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 287 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 196 – 198 | TXY | 3 |
Domaini
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Sequence similaritiesi
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0660. Eukaryota. ENOG410XNY0. LUCA. |
HOGENOMi | HOG000233024. |
InParanoidi | Q39023. |
KOi | K20536. |
OMAi | DVIDTIC. |
OrthoDBi | EOG09360BWL. |
PhylomeDBi | Q39023. |
Family and domain databases
InterProi | View protein in InterPro IPR011009. Kinase-like_dom_sf. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. |
Pfami | View protein in Pfam PF00069. Pkinase. 1 hit. |
SMARTi | View protein in SMART SM00220. S_TKc. 1 hit. |
SUPFAMi | SSF56112. SSF56112. 1 hit. |
PROSITEi | View protein in PROSITE PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q39023-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTGGGQYTD FPAVETHGGQ FISYDIFGSL FEITSKYRPP IIPIGRGAYG
60 70 80 90 100
IVCSVLDTET NELVAMKKIA NAFDNHMDAK RTLREIKLLR HLDHENIIAI
110 120 130 140 150
RDVVPPPLRR QFSDVYISTE LMDTDLHQII RSNQSLSEEH CQYFLYQLLR
160 170 180 190 200
GLKYIHSANI IHRDLKPSNL LLNANCDLKI CDFGLARPTS ENDFMTEYVV
210 220 230 240 250
TRWYRAPELL LNSSDYTAAI DVWSVGCIFM ELMNRKPLFP GKDHVHQMRL
260 270 280 290 300
LTELLGTPTE SDLGFTHNED AKRYIRQLPN FPRQPLAKLF SHVNPMAIDL
310 320 330 340 350
VDRMLTFDPN RRITVEQALN HQYLAKLHDP NDEPICQKPF SFEFEQQPLD
360 370
EEQIKEMIYQ EAIALNPTYG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | E → D in BAA04866 (PubMed:8282107).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D21839 mRNA. Translation: BAA04866.1. AL138657 Genomic DNA. Translation: CAB75493.1. AL157735 Genomic DNA. No translation available. CP002686 Genomic DNA. Translation: AEE78054.1. AF386961 mRNA. Translation: AAK62406.1. BT000007 mRNA. Translation: AAN15326.1. |
PIRi | S40469. T47504. |
RefSeqi | NP_190150.1. NM_114433.3. |
UniGenei | At.263. |
Genome annotation databases
EnsemblPlantsi | AT3G45640.1; AT3G45640.1; AT3G45640. |
GeneIDi | 823706. |
Gramenei | AT3G45640.1; AT3G45640.1; AT3G45640. |
KEGGi | ath:AT3G45640. |
Similar proteinsi
Entry informationi
Entry namei | MPK3_ARATH | |
Accessioni | Q39023Primary (citable) accession number: Q39023 Secondary accession number(s): Q9M1E3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | June 6, 2002 | |
Last modified: | March 28, 2018 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |