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Protein

Mitogen-activated protein kinase 3

Gene

MPK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Mediates the phosphorylation of VIP1 and subsequent stress genes transcription in response to Agrobacterium. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.9 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Activated by MAP kinase kinases MKK4, MKK5, MKK7 and MKK9. Activated in response to hydrogen peroxide, ozone, salt stress and flagellin bacterial elicitor. Triggered by Agrobacterium upon T-DNA transfer. Repressed by DSPTP1B/MKP2-mediated dephosphorylation.8 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67ATPPROSITE-ProRule annotation1
Active sitei164Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 52ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: GO_Central
  • protein kinase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • camalexin biosynthetic process Source: TAIR
  • defense response to bacterium Source: EnsemblPlants
  • defense response to fungus Source: EnsemblPlants
  • inflorescence development Source: TAIR
  • plant ovule development Source: TAIR
  • plant-type hypersensitive response Source: UniProtKB-KW
  • pollen development Source: TAIR
  • pollen tube guidance Source: TAIR
  • positive regulation of response to salt stress Source: EnsemblPlants
  • priming of cellular response to stress Source: TAIR
  • protein phosphorylation Source: TAIR
  • regulation of gene expression Source: GO_Central
  • response to bacterium Source: TAIR
  • response to chitin Source: TAIR
  • response to cold Source: TAIR
  • response to freezing Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to UV-B Source: TAIR
  • response to water deprivation Source: EnsemblPlants
  • response to wounding Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processHypersensitive response, Plant defense, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 399
ReactomeiR-ATH-198753 ERK/MAPK targets
R-ATH-198765 Signalling to ERK5
R-ATH-202670 ERKs are inactivated
R-ATH-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-ATH-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 3 (EC:2.7.11.24)
Short name:
AtMPK3
Short name:
MAP kinase 3
Gene namesi
Name:MPK3
Ordered Locus Names:At3g45640
ORF Names:F9K21.220, T6D9.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G45640
TAIRilocus:2085632 AT3G45640

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863121 – 370Mitogen-activated protein kinase 3Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196PhosphothreonineBy similarity1
Modified residuei198PhosphotyrosineBy similarity1
Modified residuei201PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-196 and Tyr-198, which activates the enzyme (By similarity). Dephosphorylated by DSPTP1B/MKP2.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39023
PRIDEiQ39023

PTM databases

iPTMnetiQ39023

Expressioni

Inductioni

By touch, cold, salinity stress and ozone.2 Publications

Gene expression databases

ExpressionAtlasiQ39023 baseline and differential
GenevisibleiQ39023 AT

Interactioni

Subunit structurei

Interacts with DSPTP1B/MKP2, NDPK2 and VIP1. The interaction with DSPTP1B/MKP2 is repressed by fungal elicitation (PubMed:12506203, PubMed:17947581, PubMed:20626661). Binds to LIP5 (PubMed:25010425). Interacts with VQ4 (PubMed:24750137). Interacts with RACK1A, RACK1B and RACK1C (PubMed:25731164).6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi9026, 39 interactors
DIPiDIP-768N
ELMiQ39023
IntActiQ39023, 18 interactors
MINTiQ39023
STRINGi3702.AT3G45640.1

Structurei

3D structure databases

ProteinModelPortaliQ39023
SMRiQ39023
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 324Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi196 – 198TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ39023
KOiK20536
OMAiDVIDTIC
OrthoDBiEOG09360BWL
PhylomeDBiQ39023

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q39023-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTGGGQYTD FPAVETHGGQ FISYDIFGSL FEITSKYRPP IIPIGRGAYG
60 70 80 90 100
IVCSVLDTET NELVAMKKIA NAFDNHMDAK RTLREIKLLR HLDHENIIAI
110 120 130 140 150
RDVVPPPLRR QFSDVYISTE LMDTDLHQII RSNQSLSEEH CQYFLYQLLR
160 170 180 190 200
GLKYIHSANI IHRDLKPSNL LLNANCDLKI CDFGLARPTS ENDFMTEYVV
210 220 230 240 250
TRWYRAPELL LNSSDYTAAI DVWSVGCIFM ELMNRKPLFP GKDHVHQMRL
260 270 280 290 300
LTELLGTPTE SDLGFTHNED AKRYIRQLPN FPRQPLAKLF SHVNPMAIDL
310 320 330 340 350
VDRMLTFDPN RRITVEQALN HQYLAKLHDP NDEPICQKPF SFEFEQQPLD
360 370
EEQIKEMIYQ EAIALNPTYG
Length:370
Mass (Da):42,717
Last modified:June 6, 2002 - v2
Checksum:i6992A7D97F3C9841
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15E → D in BAA04866 (PubMed:8282107).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21839 mRNA Translation: BAA04866.1
AL138657 Genomic DNA Translation: CAB75493.1
AL157735 Genomic DNA No translation available.
CP002686 Genomic DNA Translation: AEE78054.1
AF386961 mRNA Translation: AAK62406.1
BT000007 mRNA Translation: AAN15326.1
PIRiS40469
T47504
RefSeqiNP_190150.1, NM_114433.3
UniGeneiAt.263

Genome annotation databases

EnsemblPlantsiAT3G45640.1; AT3G45640.1; AT3G45640
GeneIDi823706
GrameneiAT3G45640.1; AT3G45640.1; AT3G45640
KEGGiath:AT3G45640

Similar proteinsi

Entry informationi

Entry nameiMPK3_ARATH
AccessioniPrimary (citable) accession number: Q39023
Secondary accession number(s): Q9M1E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: May 23, 2018
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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