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Q39022 (MPK2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 2

Short name=AtMPK2
Short name=MAP kinase 2
EC=2.7.11.24
Gene names
Name:MPK2
Ordered Locus Names:At1g59580
ORF Names:T30E16.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation Probable. Ref.1

Subunit structure

Interacts with MKK3. Ref.7

Tissue specificity

Highest levels in the stem. Present in the leaf, root and flower, but not in seeds. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity. Phosphorylated on Ser residue. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 376376Mitogen-activated protein kinase 2
PRO_0000186311

Regions

Domain32 – 319288Protein kinase
Nucleotide binding38 – 469ATP By similarity
Motif191 – 1933TXY

Sites

Active site1581Proton acceptor By similarity
Binding site611ATP By similarity

Amino acid modifications

Modified residue1911Phosphothreonine By similarity
Modified residue1931Phosphotyrosine By similarity
Modified residue1961Phosphothreonine By similarity

Experimental info

Sequence conflict331V → M in BAA03536. Ref.1
Sequence conflict2991M → I in BAA03536. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q39022 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: EDA07145A32CE25D

FASTA37643,125
        10         20         30         40         50         60 
MATPVDPPNG IRNQGKHYFS MWQTLFEIDT KYVPIKPIGR GAYGVVCSSV NRESNERVAI 

        70         80         90        100        110        120 
KKIHNVFENR IDALRTLREL KLLRHLRHEN VVALKDVMMA NHKRSFKDVY LVYELMDTDL 

       130        140        150        160        170        180 
HQIIKSSQVL SNDHCQYFLF QLLRGLKYIH SANILHRDLK PGNLLVNANC DLKICDFGLA 

       190        200        210        220        230        240 
RTSNTKGQFM TEYVVTRWYR APELLLCCDN YGTSIDVWSV GCIFAELLGR KPVFPGTECL 

       250        260        270        280        290        300 
NQIKLIINIL GSQREEDLEF IDNPKAKRYI ESLPYSPGIS FSRLYPGANV LAIDLLQKML 

       310        320        330        340        350        360 
VLDPSKRISV TEALQHPYMA PLYDPSANPP AQVPIDLDVD EDEDLGAEMI RELMWKEMIH 

       370 
YHPEAATINN NEVSEF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells."
Mizoguchi T., Gotoh Y., Nishida E., Yamaguchi-Shinozaki K., Hayashida N., Iwasaki T., Kamada H., Shinozaki K.
Plant J. 5:111-122(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, TISSUE SPECIFICITY, PHOSPHORYLATION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[7]"The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling."
Doczi R., Brader G., Pettko-Szandtner A., Rajh I., Djamei A., Pitzschke A., Teige M., Hirt H.
Plant Cell 19:3266-3279(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14714 mRNA. Translation: BAA03536.1.
AC009317 Genomic DNA. Translation: AAF79750.1.
CP002684 Genomic DNA. Translation: AEE33590.1.
CP002684 Genomic DNA. Translation: AEE33591.1.
AY035134 mRNA. Translation: AAK59639.1.
AY113911 mRNA. Translation: AAM44959.1.
PIRF96619.
RefSeqNP_564746.1. NM_104651.4.
NP_974049.1. NM_202320.1.
UniGeneAt.262.

3D structure databases

ProteinModelPortalQ39022.
SMRQ39022. Positions 25-363.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid27473. 1 interaction.
IntActQ39022. 2 interactions.
STRING3702.AT1G59580.2-P.

Proteomic databases

PRIDEQ39022.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G59580.1; AT1G59580.1; AT1G59580.
AT1G59580.2; AT1G59580.2; AT1G59580.
GeneID842248.
KEGGath:AT1G59580.

Organism-specific databases

GeneFarm854. 89.
TAIRAT1G59580.

Phylogenomic databases

HOGENOMHOG000233024.
InParanoidQ39022.
KOK04371.
OMANRESNER.
PhylomeDBQ39022.

Enzyme and pathway databases

BioCycARA:AT1G59580-MONOMER.
ARA:GQT-1108-MONOMER.
BRENDA2.7.11.24. 399.

Gene expression databases

GenevestigatorQ39022.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK2_ARATH
AccessionPrimary (citable) accession number: Q39022
Secondary accession number(s): Q9LQ58
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names