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Q39022

- MPK2_ARATH

UniProt

Q39022 - MPK2_ARATH

Protein

Mitogen-activated protein kinase 2

Gene

MPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (06 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611ATPPROSITE-ProRule annotation
    Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC
    3. protein kinase activity Source: TAIR

    GO - Biological processi

    1. protein phosphorylation Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G59580-MONOMER.
    ARA:GQT-1108-MONOMER.
    BRENDAi2.7.11.24. 399.
    ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 2 (EC:2.7.11.24)
    Short name:
    AtMPK2
    Short name:
    MAP kinase 2
    Gene namesi
    Name:MPK2
    Ordered Locus Names:At1g59580
    ORF Names:T30E16.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G59580.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 376376Mitogen-activated protein kinase 2PRO_0000186311Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911PhosphothreonineBy similarity
    Modified residuei193 – 1931PhosphotyrosineBy similarity
    Modified residuei196 – 1961PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity. Phosphorylated on Ser residue.By similarity1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ39022.

    Expressioni

    Tissue specificityi

    Highest levels in the stem. Present in the leaf, root and flower, but not in seeds.1 Publication

    Gene expression databases

    GenevestigatoriQ39022.

    Interactioni

    Subunit structurei

    Interacts with MKK3.1 Publication

    Protein-protein interaction databases

    BioGridi27473. 1 interaction.
    IntActiQ39022. 2 interactions.
    STRINGi3702.AT1G59580.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39022.
    SMRiQ39022. Positions 25-363.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi191 – 1933TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000233024.
    InParanoidiQ39022.
    KOiK04371.
    OMAiNRESNER.
    PhylomeDBiQ39022.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q39022-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPVDPPNG IRNQGKHYFS MWQTLFEIDT KYVPIKPIGR GAYGVVCSSV    50
    NRESNERVAI KKIHNVFENR IDALRTLREL KLLRHLRHEN VVALKDVMMA 100
    NHKRSFKDVY LVYELMDTDL HQIIKSSQVL SNDHCQYFLF QLLRGLKYIH 150
    SANILHRDLK PGNLLVNANC DLKICDFGLA RTSNTKGQFM TEYVVTRWYR 200
    APELLLCCDN YGTSIDVWSV GCIFAELLGR KPVFPGTECL NQIKLIINIL 250
    GSQREEDLEF IDNPKAKRYI ESLPYSPGIS FSRLYPGANV LAIDLLQKML 300
    VLDPSKRISV TEALQHPYMA PLYDPSANPP AQVPIDLDVD EDEDLGAEMI 350
    RELMWKEMIH YHPEAATINN NEVSEF 376
    Length:376
    Mass (Da):43,125
    Last modified:June 6, 2002 - v2
    Checksum:iEDA07145A32CE25D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331V → M in BAA03536. (PubMed:8130795)Curated
    Sequence conflicti299 – 2991M → I in BAA03536. (PubMed:8130795)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14714 mRNA. Translation: BAA03536.1.
    AC009317 Genomic DNA. Translation: AAF79750.1.
    CP002684 Genomic DNA. Translation: AEE33590.1.
    CP002684 Genomic DNA. Translation: AEE33591.1.
    AY035134 mRNA. Translation: AAK59639.1.
    AY113911 mRNA. Translation: AAM44959.1.
    PIRiF96619.
    RefSeqiNP_564746.1. NM_104651.4.
    NP_974049.1. NM_202320.1.
    UniGeneiAt.262.

    Genome annotation databases

    EnsemblPlantsiAT1G59580.1; AT1G59580.1; AT1G59580.
    AT1G59580.2; AT1G59580.2; AT1G59580.
    GeneIDi842248.
    KEGGiath:AT1G59580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14714 mRNA. Translation: BAA03536.1 .
    AC009317 Genomic DNA. Translation: AAF79750.1 .
    CP002684 Genomic DNA. Translation: AEE33590.1 .
    CP002684 Genomic DNA. Translation: AEE33591.1 .
    AY035134 mRNA. Translation: AAK59639.1 .
    AY113911 mRNA. Translation: AAM44959.1 .
    PIRi F96619.
    RefSeqi NP_564746.1. NM_104651.4.
    NP_974049.1. NM_202320.1.
    UniGenei At.262.

    3D structure databases

    ProteinModelPortali Q39022.
    SMRi Q39022. Positions 25-363.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27473. 1 interaction.
    IntActi Q39022. 2 interactions.
    STRINGi 3702.AT1G59580.2-P.

    Proteomic databases

    PRIDEi Q39022.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G59580.1 ; AT1G59580.1 ; AT1G59580 .
    AT1G59580.2 ; AT1G59580.2 ; AT1G59580 .
    GeneIDi 842248.
    KEGGi ath:AT1G59580.

    Organism-specific databases

    GeneFarmi 854. 89.
    TAIRi AT1G59580.

    Phylogenomic databases

    HOGENOMi HOG000233024.
    InParanoidi Q39022.
    KOi K04371.
    OMAi NRESNER.
    PhylomeDBi Q39022.

    Enzyme and pathway databases

    BioCyci ARA:AT1G59580-MONOMER.
    ARA:GQT-1108-MONOMER.
    BRENDAi 2.7.11.24. 399.
    Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Gene expression databases

    Genevestigatori Q39022.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells."
      Mizoguchi T., Gotoh Y., Nishida E., Yamaguchi-Shinozaki K., Hayashida N., Iwasaki T., Kamada H., Shinozaki K.
      Plant J. 5:111-122(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, TISSUE SPECIFICITY, PHOSPHORYLATION.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. Cited for: GENE FAMILY.
    7. "The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling."
      Doczi R., Brader G., Pettko-Szandtner A., Rajh I., Djamei A., Pitzschke A., Teige M., Hirt H.
      Plant Cell 19:3266-3279(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKK3.

    Entry informationi

    Entry nameiMPK2_ARATH
    AccessioniPrimary (citable) accession number: Q39022
    Secondary accession number(s): Q9LQ58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: June 6, 2002
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3