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Protein

Mitogen-activated protein kinase 1

Gene

MPK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: TAIR
  • regulation of gene expression Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-375165. NCAM signaling for neurite out-growth.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-444257. RSK activation.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-5675221. Negative regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-881907. Gastrin-CREB signalling pathway via PKC and MAPK.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 1 (EC:2.7.11.24)
Short name:
AtMPK1
Short name:
MAP kinase 1
Gene namesi
Name:MPK1
Ordered Locus Names:At1g10210
ORF Names:F14N23.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2012808. AT1G10210.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863101 – 370Mitogen-activated protein kinase 1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphothreonineBy similarity1
Modified residuei193PhosphotyrosineBy similarity1
Modified residuei196PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme (By similarity). Autophosphorylated on threonine and tyrosine residues (Probable). Phosphorylated on Ser residue.By similarityCurated1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39021.
PRIDEiQ39021.

PTM databases

iPTMnetiQ39021.

Expressioni

Tissue specificityi

Highest levels in the stem. Present in the leaf, root and flower, but not in seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ39021. baseline and differential.
GenevisibleiQ39021. AT.

Interactioni

Subunit structurei

Interacts with MKK3.1 Publication

Protein-protein interaction databases

BioGridi22799. 4 interactors.
IntActiQ39021. 3 interactors.
STRINGi3702.AT1G10210.1.

Structurei

3D structure databases

ProteinModelPortaliQ39021.
SMRiQ39021.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39021.
KOiK20535.
OMAiCEEALEH.
OrthoDBiEOG09360CTC.
PhylomeDBiQ39021.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLVDPPNG IRNEGKHYFS MWQTLFEIDT KYMPIKPIGR GAYGVVCSSV
60 70 80 90 100
NSDTNEKVAI KKIHNVYENR IDALRTLREL KLLRHLRHEN VIALKDVMMP
110 120 130 140 150
IHKMSFKDVY LVYELMDTDL HQIIKSSQVL SNDHCQYFLF QLLRGLKYIH
160 170 180 190 200
SANILHRDLK PGNLLVNANC DLKICDFGLA RASNTKGQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFQGTECL NQLKLIVNIL
260 270 280 290 300
GSQREEDLEF IDNPKAKRYI RSLPYSPGMS LSRLYPGAHV LAIDLLQKML
310 320 330 340 350
VFDPSKRISV SEALQHPYMA PLYDPNANPP AQVPIDLDVD EDLREEMIRE
360 370
MMWNEMLHYH PQASTLNTEL
Length:370
Mass (Da):42,644
Last modified:June 6, 2002 - v2
Checksum:iF2962D7881C87A1B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129V → R in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti250L → I in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti262D → V in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti287G → C in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti310V → A in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti352M → I in BAA03535 (PubMed:8130795).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14713 mRNA. Translation: BAA03535.1.
AC005489 Genomic DNA. Translation: AAD32871.1.
CP002684 Genomic DNA. Translation: AEE28553.1.
CP002684 Genomic DNA. Translation: AEE28554.1.
CP002684 Genomic DNA. Translation: ANM58066.1.
AY059937 mRNA. Translation: AAL24419.1.
BT000062 mRNA. Translation: AAN15381.1.
PIRiF86236.
RefSeqiNP_001031017.1. NM_001035940.2.
NP_001320530.1. NM_001331892.1.
NP_172492.1. NM_100895.3.
UniGeneiAt.261.

Genome annotation databases

EnsemblPlantsiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
AT1G10210.3; AT1G10210.3; AT1G10210.
GeneIDi837559.
GrameneiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
AT1G10210.3; AT1G10210.3; AT1G10210.
KEGGiath:AT1G10210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14713 mRNA. Translation: BAA03535.1.
AC005489 Genomic DNA. Translation: AAD32871.1.
CP002684 Genomic DNA. Translation: AEE28553.1.
CP002684 Genomic DNA. Translation: AEE28554.1.
CP002684 Genomic DNA. Translation: ANM58066.1.
AY059937 mRNA. Translation: AAL24419.1.
BT000062 mRNA. Translation: AAN15381.1.
PIRiF86236.
RefSeqiNP_001031017.1. NM_001035940.2.
NP_001320530.1. NM_001331892.1.
NP_172492.1. NM_100895.3.
UniGeneiAt.261.

3D structure databases

ProteinModelPortaliQ39021.
SMRiQ39021.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22799. 4 interactors.
IntActiQ39021. 3 interactors.
STRINGi3702.AT1G10210.1.

PTM databases

iPTMnetiQ39021.

Proteomic databases

PaxDbiQ39021.
PRIDEiQ39021.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
AT1G10210.3; AT1G10210.3; AT1G10210.
GeneIDi837559.
GrameneiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
AT1G10210.3; AT1G10210.3; AT1G10210.
KEGGiath:AT1G10210.

Organism-specific databases

AraportiAT1G10210.
TAIRilocus:2012808. AT1G10210.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39021.
KOiK20535.
OMAiCEEALEH.
OrthoDBiEOG09360CTC.
PhylomeDBiQ39021.

Enzyme and pathway databases

BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-375165. NCAM signaling for neurite out-growth.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-444257. RSK activation.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-5675221. Negative regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-881907. Gastrin-CREB signalling pathway via PKC and MAPK.

Miscellaneous databases

PROiPR:Q39021.

Gene expression databases

ExpressionAtlasiQ39021. baseline and differential.
GenevisibleiQ39021. AT.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMPK1_ARATH
AccessioniPrimary (citable) accession number: Q39021
Secondary accession number(s): Q9SY63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: April 12, 2017
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.