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Protein

Mitogen-activated protein kinase 1

Gene

MPK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ATPPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G10210-MONOMER.
ARA:GQT-430-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 1 (EC:2.7.11.24)
Short name:
AtMPK1
Short name:
MAP kinase 1
Gene namesi
Name:MPK1
Ordered Locus Names:At1g10210
ORF Names:F14N23.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G10210.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Mitogen-activated protein kinase 1PRO_0000186310Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphothreonineBy similarity
Modified residuei193 – 1931PhosphotyrosineBy similarity
Modified residuei196 – 1961PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme (By similarity). Autophosphorylated on threonine and tyrosine residues (Probable). Phosphorylated on Ser residue.By similarityCurated1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39021.
PRIDEiQ39021.

PTM databases

iPTMnetiQ39021.

Expressioni

Tissue specificityi

Highest levels in the stem. Present in the leaf, root and flower, but not in seeds.1 Publication

Gene expression databases

GenevisibleiQ39021. AT.

Interactioni

Subunit structurei

Interacts with MKK3.1 Publication

Protein-protein interaction databases

BioGridi22799. 4 interactions.
IntActiQ39021. 3 interactions.
STRINGi3702.AT1G10210.1.

Structurei

3D structure databases

ProteinModelPortaliQ39021.
SMRiQ39021. Positions 23-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39021.
KOiK04371.
OMAiCEEALEH.
OrthoDBiEOG09360CTC.
PhylomeDBiQ39021.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLVDPPNG IRNEGKHYFS MWQTLFEIDT KYMPIKPIGR GAYGVVCSSV
60 70 80 90 100
NSDTNEKVAI KKIHNVYENR IDALRTLREL KLLRHLRHEN VIALKDVMMP
110 120 130 140 150
IHKMSFKDVY LVYELMDTDL HQIIKSSQVL SNDHCQYFLF QLLRGLKYIH
160 170 180 190 200
SANILHRDLK PGNLLVNANC DLKICDFGLA RASNTKGQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFQGTECL NQLKLIVNIL
260 270 280 290 300
GSQREEDLEF IDNPKAKRYI RSLPYSPGMS LSRLYPGAHV LAIDLLQKML
310 320 330 340 350
VFDPSKRISV SEALQHPYMA PLYDPNANPP AQVPIDLDVD EDLREEMIRE
360 370
MMWNEMLHYH PQASTLNTEL
Length:370
Mass (Da):42,644
Last modified:June 6, 2002 - v2
Checksum:iF2962D7881C87A1B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291V → R in BAA03535 (PubMed:8130795).Curated
Sequence conflicti250 – 2501L → I in BAA03535 (PubMed:8130795).Curated
Sequence conflicti262 – 2621D → V in BAA03535 (PubMed:8130795).Curated
Sequence conflicti287 – 2871G → C in BAA03535 (PubMed:8130795).Curated
Sequence conflicti310 – 3101V → A in BAA03535 (PubMed:8130795).Curated
Sequence conflicti352 – 3521M → I in BAA03535 (PubMed:8130795).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14713 mRNA. Translation: BAA03535.1.
AC005489 Genomic DNA. Translation: AAD32871.1.
CP002684 Genomic DNA. Translation: AEE28553.1.
CP002684 Genomic DNA. Translation: AEE28554.1.
AY059937 mRNA. Translation: AAL24419.1.
BT000062 mRNA. Translation: AAN15381.1.
PIRiF86236.
RefSeqiNP_001031017.1. NM_001035940.1.
NP_172492.1. NM_100895.2.
UniGeneiAt.261.

Genome annotation databases

EnsemblPlantsiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
GeneIDi837559.
GrameneiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
KEGGiath:AT1G10210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14713 mRNA. Translation: BAA03535.1.
AC005489 Genomic DNA. Translation: AAD32871.1.
CP002684 Genomic DNA. Translation: AEE28553.1.
CP002684 Genomic DNA. Translation: AEE28554.1.
AY059937 mRNA. Translation: AAL24419.1.
BT000062 mRNA. Translation: AAN15381.1.
PIRiF86236.
RefSeqiNP_001031017.1. NM_001035940.1.
NP_172492.1. NM_100895.2.
UniGeneiAt.261.

3D structure databases

ProteinModelPortaliQ39021.
SMRiQ39021. Positions 23-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22799. 4 interactions.
IntActiQ39021. 3 interactions.
STRINGi3702.AT1G10210.1.

PTM databases

iPTMnetiQ39021.

Proteomic databases

PaxDbiQ39021.
PRIDEiQ39021.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
GeneIDi837559.
GrameneiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
KEGGiath:AT1G10210.

Organism-specific databases

TAIRiAT1G10210.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ39021.
KOiK04371.
OMAiCEEALEH.
OrthoDBiEOG09360CTC.
PhylomeDBiQ39021.

Enzyme and pathway databases

BioCyciARA:AT1G10210-MONOMER.
ARA:GQT-430-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Miscellaneous databases

PROiQ39021.

Gene expression databases

GenevisibleiQ39021. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK1_ARATH
AccessioniPrimary (citable) accession number: Q39021
Secondary accession number(s): Q9SY63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: September 7, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.