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Protein

Mitogen-activated protein kinase 1

Gene

MPK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: TAIR
  • regulation of gene expression Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 399
ReactomeiR-ATH-110056 MAPK3 (ERK1) activation
R-ATH-112409 RAF-independent MAPK1/3 activation
R-ATH-112411 MAPK1 (ERK2) activation
R-ATH-198753 ERK/MAPK targets
R-ATH-198765 Signalling to ERK5
R-ATH-202670 ERKs are inactivated
R-ATH-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-375165 NCAM signaling for neurite out-growth
R-ATH-442742 CREB phosphorylation through the activation of Ras
R-ATH-444257 RSK activation
R-ATH-445144 Signal transduction by L1
R-ATH-5673001 RAF/MAP kinase cascade
R-ATH-5674135 MAP2K and MAPK activation
R-ATH-5674499 Negative feedback regulation of MAPK pathway
R-ATH-6798695 Neutrophil degranulation
R-ATH-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 1 (EC:2.7.11.24)
Short name:
AtMPK1
Short name:
MAP kinase 1
Gene namesi
Name:MPK1
Ordered Locus Names:At1g10210
ORF Names:F14N23.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G10210
TAIRilocus:2012808 AT1G10210

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863101 – 370Mitogen-activated protein kinase 1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphothreonineBy similarity1
Modified residuei193PhosphotyrosineBy similarity1
Modified residuei196PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme (By similarity). Autophosphorylated on threonine and tyrosine residues (Probable). Phosphorylated on Ser residue.By similarityCurated1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39021
PRIDEiQ39021

PTM databases

iPTMnetiQ39021

Expressioni

Tissue specificityi

Highest levels in the stem. Present in the leaf, root and flower, but not in seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ39021 baseline and differential
GenevisibleiQ39021 AT

Interactioni

Subunit structurei

Interacts with MKK3.2 Publications

Protein-protein interaction databases

BioGridi22799, 4 interactors
IntActiQ39021, 3 interactors
STRINGi3702.AT1G10210.1

Structurei

3D structure databases

ProteinModelPortaliQ39021
SMRiQ39021
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ39021
KOiK20535
OMAiAYGMVSS
OrthoDBiEOG09360CTC
PhylomeDBiQ39021

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q39021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLVDPPNG IRNEGKHYFS MWQTLFEIDT KYMPIKPIGR GAYGVVCSSV
60 70 80 90 100
NSDTNEKVAI KKIHNVYENR IDALRTLREL KLLRHLRHEN VIALKDVMMP
110 120 130 140 150
IHKMSFKDVY LVYELMDTDL HQIIKSSQVL SNDHCQYFLF QLLRGLKYIH
160 170 180 190 200
SANILHRDLK PGNLLVNANC DLKICDFGLA RASNTKGQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFQGTECL NQLKLIVNIL
260 270 280 290 300
GSQREEDLEF IDNPKAKRYI RSLPYSPGMS LSRLYPGAHV LAIDLLQKML
310 320 330 340 350
VFDPSKRISV SEALQHPYMA PLYDPNANPP AQVPIDLDVD EDLREEMIRE
360 370
MMWNEMLHYH PQASTLNTEL
Length:370
Mass (Da):42,644
Last modified:June 6, 2002 - v2
Checksum:iF2962D7881C87A1B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129V → R in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti250L → I in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti262D → V in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti287G → C in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti310V → A in BAA03535 (PubMed:8130795).Curated1
Sequence conflicti352M → I in BAA03535 (PubMed:8130795).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14713 mRNA Translation: BAA03535.1
AC005489 Genomic DNA Translation: AAD32871.1
CP002684 Genomic DNA Translation: AEE28553.1
CP002684 Genomic DNA Translation: AEE28554.1
CP002684 Genomic DNA Translation: ANM58066.1
AY059937 mRNA Translation: AAL24419.1
BT000062 mRNA Translation: AAN15381.1
PIRiF86236
RefSeqiNP_001031017.1, NM_001035940.2
NP_001320530.1, NM_001331892.1
NP_172492.1, NM_100895.3
UniGeneiAt.261

Genome annotation databases

EnsemblPlantsiAT1G10210.1; AT1G10210.1; AT1G10210
AT1G10210.2; AT1G10210.2; AT1G10210
AT1G10210.3; AT1G10210.3; AT1G10210
GeneIDi837559
GrameneiAT1G10210.1; AT1G10210.1; AT1G10210
AT1G10210.2; AT1G10210.2; AT1G10210
AT1G10210.3; AT1G10210.3; AT1G10210
KEGGiath:AT1G10210

Similar proteinsi

Entry informationi

Entry nameiMPK1_ARATH
AccessioniPrimary (citable) accession number: Q39021
Secondary accession number(s): Q9SY63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: April 25, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health