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Q39021

- MPK1_ARATH

UniProt

Q39021 - MPK1_ARATH

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Protein
Mitogen-activated protein kinase 1
Gene
MPK1, At1g10210, F14N23.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation Inferred.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ATP By similarity
Active sitei158 – 1581Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. auxin-activated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G10210-MONOMER.
ARA:GQT-430-MONOMER.
BRENDAi2.7.11.24. 399.
ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 1 (EC:2.7.11.24)
Short name:
AtMPK1
Short name:
MAP kinase 1
Gene namesi
Name:MPK1
Ordered Locus Names:At1g10210
ORF Names:F14N23.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G10210.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Mitogen-activated protein kinase 1
PRO_0000186310Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911Phosphothreonine By similarity
Modified residuei193 – 1931Phosphotyrosine By similarity
Modified residuei196 – 1961Phosphothreonine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity. Autophosphorylated on threonine and tyrosine residues Inferred. Phosphorylated on Ser residue.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39021.
PRIDEiQ39021.

Expressioni

Tissue specificityi

Highest levels in the stem. Present in the leaf, root and flower, but not in seeds.1 Publication

Gene expression databases

ArrayExpressiQ39021.
GenevestigatoriQ39021.

Interactioni

Subunit structurei

Interacts with MKK3.1 Publication

Protein-protein interaction databases

BioGridi22799. 3 interactions.
IntActiQ39021. 3 interactions.
STRINGi3702.AT1G10210.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ39021.
SMRiQ39021. Positions 25-362.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 319288Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ39021.
KOiK04371.
OMAiQGLSNDH.
PhylomeDBiQ39021.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39021-1 [UniParc]FASTAAdd to Basket

« Hide

MATLVDPPNG IRNEGKHYFS MWQTLFEIDT KYMPIKPIGR GAYGVVCSSV    50
NSDTNEKVAI KKIHNVYENR IDALRTLREL KLLRHLRHEN VIALKDVMMP 100
IHKMSFKDVY LVYELMDTDL HQIIKSSQVL SNDHCQYFLF QLLRGLKYIH 150
SANILHRDLK PGNLLVNANC DLKICDFGLA RASNTKGQFM TEYVVTRWYR 200
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFQGTECL NQLKLIVNIL 250
GSQREEDLEF IDNPKAKRYI RSLPYSPGMS LSRLYPGAHV LAIDLLQKML 300
VFDPSKRISV SEALQHPYMA PLYDPNANPP AQVPIDLDVD EDLREEMIRE 350
MMWNEMLHYH PQASTLNTEL 370
Length:370
Mass (Da):42,644
Last modified:June 6, 2002 - v2
Checksum:iF2962D7881C87A1B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291V → R in BAA03535. 1 Publication
Sequence conflicti250 – 2501L → I in BAA03535. 1 Publication
Sequence conflicti262 – 2621D → V in BAA03535. 1 Publication
Sequence conflicti287 – 2871G → C in BAA03535. 1 Publication
Sequence conflicti310 – 3101V → A in BAA03535. 1 Publication
Sequence conflicti352 – 3521M → I in BAA03535. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14713 mRNA. Translation: BAA03535.1.
AC005489 Genomic DNA. Translation: AAD32871.1.
CP002684 Genomic DNA. Translation: AEE28553.1.
CP002684 Genomic DNA. Translation: AEE28554.1.
AY059937 mRNA. Translation: AAL24419.1.
BT000062 mRNA. Translation: AAN15381.1.
PIRiF86236.
RefSeqiNP_001031017.1. NM_001035940.1.
NP_172492.1. NM_100895.2.
UniGeneiAt.261.

Genome annotation databases

EnsemblPlantsiAT1G10210.1; AT1G10210.1; AT1G10210.
AT1G10210.2; AT1G10210.2; AT1G10210.
GeneIDi837559.
KEGGiath:AT1G10210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14713 mRNA. Translation: BAA03535.1 .
AC005489 Genomic DNA. Translation: AAD32871.1 .
CP002684 Genomic DNA. Translation: AEE28553.1 .
CP002684 Genomic DNA. Translation: AEE28554.1 .
AY059937 mRNA. Translation: AAL24419.1 .
BT000062 mRNA. Translation: AAN15381.1 .
PIRi F86236.
RefSeqi NP_001031017.1. NM_001035940.1.
NP_172492.1. NM_100895.2.
UniGenei At.261.

3D structure databases

ProteinModelPortali Q39021.
SMRi Q39021. Positions 25-362.
ModBasei Search...

Protein-protein interaction databases

BioGridi 22799. 3 interactions.
IntActi Q39021. 3 interactions.
STRINGi 3702.AT1G10210.1-P.

Proteomic databases

PaxDbi Q39021.
PRIDEi Q39021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G10210.1 ; AT1G10210.1 ; AT1G10210 .
AT1G10210.2 ; AT1G10210.2 ; AT1G10210 .
GeneIDi 837559.
KEGGi ath:AT1G10210.

Organism-specific databases

GeneFarmi 855. 89.
TAIRi AT1G10210.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q39021.
KOi K04371.
OMAi QGLSNDH.
PhylomeDBi Q39021.

Enzyme and pathway databases

BioCyci ARA:AT1G10210-MONOMER.
ARA:GQT-430-MONOMER.
BRENDAi 2.7.11.24. 399.
Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Gene expression databases

ArrayExpressi Q39021.
Genevestigatori Q39021.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells."
    Mizoguchi T., Gotoh Y., Nishida E., Yamaguchi-Shinozaki K., Hayashida N., Iwasaki T., Kamada H., Shinozaki K.
    Plant J. 5:111-122(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, TISSUE SPECIFICITY, PHOSPHORYLATION.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: GENE FAMILY.
  7. "The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling."
    Doczi R., Brader G., Pettko-Szandtner A., Rajh I., Djamei A., Pitzschke A., Teige M., Hirt H.
    Plant Cell 19:3266-3279(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK3.

Entry informationi

Entry nameiMPK1_ARATH
AccessioniPrimary (citable) accession number: Q39021
Secondary accession number(s): Q9SY63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: September 3, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi