Reviewed, UniProtKB/Swiss-Prot Q39019 (KSG10_ARATH)

Names and origin

Protein names

Recommended name:
    Shaggy-related protein kinase kappa
    EC=2.7.11.1
Alternative name(s):
    ASK-kappa
      Short name=AtK-1

Gene names

Name:	ASK10
Ordered Locus Names:	At1g09840
ORF Names:	F21M12.23

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May mediate extracellular signals to regulate transcription in differentiating cells By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Expressed exclusively in inflorescences.
Post-translational modification	Autophosphorylated mainly on threonine and serine residues By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAB60754.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q2V412	1	EBI-1238904,EBI-1238781
CALM	P62157	1	EBI-1238904,EBI-397403	From a different organism.
CAM1	P25854	1	EBI-1238904,EBI-1235664

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Shaggy-related protein kinase kappa		PRO_0000086225
Regions
	Domain	83 – 367	285	Protein kinase
	Nucleotide binding	89 – 97	9	ATP By similarity
Sites
	Active site	208	1	Proton acceptor By similarity
	Binding site	112	1	ATP By similarity
Experimental info
	Sequence conflict	313	1	P → G Ref.1
	Sequence conflict	313	1	P → G Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q39019-1 [UniParc]. Last modified November 21, 2003. Version 2. Checksum: 5810423888792453	FASTA	421	47,669
	10 20 30 40 50 60 MASSGLGNGV GTSRSAKGLK SSSSSVDWLT RDLAETRIRD KVETDDERDS EPDIIDGAGA 70 80 90 100 110 120 EPGHVIRTTL RGRNGQSRQT VSYISEHVVG TGSFGMVFQA KCRETGEVVA IKKVLQDKRY 130 140 150 160 170 180 KNRELQIMQM LDHPNAVALK HSFFSRTDNE EVYLNLVLEF VPETVNRVAR SYSRTNQLMP 190 200 210 220 230 240 LIYVKLYTYQ ICRALAYIHN SFGLCHRDIK PQNLLVNPHT HQLKICDFGS AKVLVKGEPN 250 260 270 280 290 300 VSYICSRYYR APELIFGASE YTTAIDIWST GCVMAELLLG QPLFPGESGV DQLVEIIKVL 310 320 330 340 350 360 GTPTREEIKC MNPNYTEFKF PQIKPHPWHK VFQKRLPPEA VDLLCRFFQY SPNLRCTALE 370 380 390 400 410 420 ACIHPLFDEL RDPNTRLPNG RPLPPLFNFK PQELSGIPPE IVNRLVPEHA RKQNLFMALH S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inflorescence-specific expression of AtK-1, a novel Arabidopsis thaliana homologue of shaggy/glycogen synthase kinase-3." Jonak C., Heberle-Bors E., Hirt H. Plant Mol. Biol. 27:217-221(1995) [PubMed: 7865793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	Carnier M.C., Kreis M., Dornelas M.C. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 47-421. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79279 Genomic DNA. Translation: CAA55866.1. AC000132 Genomic DNA. Translation: AAB60754.1. Sequence problems. AY092987 mRNA. Translation: AAM12986.1. BT000132 mRNA. Translation: AAN15451.1. Y07597 mRNA. Translation: CAA68872.1.
IPI	IPI00539207.
PIR	F86232. S51938.
RefSeq	NP_001031013.1. NP_001077498.1. NP_001077499.1. NP_172455.1. NP_849627.1. NP_973801.1.
UniGene	At.24571
3D structure databases
HSSP	HSSP built from PDB template 1I09 based on UniProtKB P49841.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39019. 3 interactions.
Genome annotation databases
GeneID	837516.
GenomeReviews	Gene locus AT1G09840 in contig CT485782_GR.
KEGG	ath:AT1G09840.
NMPDR	fig|3702.1.peg.1216.
Organism-specific databases
GeneFarm	1535. 93.
TAIR	At1g09840.
Phylogenomic databases
OMA	Q39019. DQQVEIS.
Enzyme and pathway databases
BRENDA	2.7.11.1. 302. 2.7.11.26. 302.
Gene expression databases
ArrayExpress	Q39019.
GermOnline	AT1G09840. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KSG10_ARATH

Accession

Primary (citable) accession number: Q39019
Secondary accession number(s): O04506, Q96244

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 21, 2003
Last modified:	June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)