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Q39017

- DGK1_ARATH

UniProt

Q39017 - DGK1_ARATH

Protein

Diacylglycerol kinase 1

Gene

DGK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (27 Mar 2002)
      Previous versions | rss
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    Functioni

    Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri79 – 13759Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri149 – 21264Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: TAIR
    3. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. intracellular signal transduction Source: InterPro
    3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Plant defense, Stress response

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT5G07920-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase 1 (EC:2.7.1.107)
    Short name:
    AtDGK1
    Short name:
    DAG kinase 1
    Alternative name(s):
    Diglyceride kinase 1
    Short name:
    DGK 1
    Gene namesi
    Name:DGK1
    Ordered Locus Names:At5g07920
    ORF Names:F13G24.120, MXM12.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G07920.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 728728Diacylglycerol kinase 1PRO_0000218475Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki491 – 491Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki500 – 500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiQ39017.
    PRIDEiQ39017.

    Expressioni

    Tissue specificityi

    Expressed in roots, shoots, and leaves.1 Publication

    Inductioni

    By volatile C6-aldehydes ((E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenol), and allo-ocimene.1 Publication

    Gene expression databases

    GenevestigatoriQ39017.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT5G07920.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39017.
    SMRiQ39017. Positions 78-137, 145-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei27 – 4822HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini357 – 496140DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri79 – 13759Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri149 – 21264Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    HOGENOMiHOG000005923.
    InParanoidiQ39017.
    KOiK00901.
    OMAiTISHHGQ.
    PhylomeDBiQ39017.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q39017-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDDGELGMF FPSWTSKNPI DTVESRGLMF SCFVAALVGI LTIAYTAFQW    50
    RRNINLSWTK AIARSKKNPK ARHKVPVAPH SWELDPIARA KNLNCCVCLK 100
    SMSPSQAIVA SESFFHRCTI CGAAAHFNCS SSAPKDCKCV SMVGFEHVVH 150
    QWAVRWTEGA DQTDDSSFCS YCDESCSSSF LGGSPIWCCL WCQRLVHVDC 200
    HSNMSNETGD ICDLGPLRRL ILCPLYVKEL TRNPSGGFLS SITHGANELA 250
    STALASIRIQ SKKYKQTNET SADTGNSGSN CDESTESTAD TGPTVNGAHA 300
    VLENSISVMN GDSSNGDSDS NGKLEKKPSV KRTGSFGQKE YHALRSKLKY 350
    ELADLPSDAR PLLVFINKKS GAQRGDSLRQ RLHLHLNPVQ VFELSSVQGP 400
    EVGLFLFRKV PHFRVLVCGG DGTAGWVLDA IEKQNFISPP AVAILPAGTG 450
    NDLSRVLNWG GGLGSVERQG GLSTVLQNIE HAAVTVLDRW KVSILNQQGK 500
    QLQPPKYMNN YIGVGCDAKV ALEIHNLREE NPERFYSQFM NKVLYAREGA 550
    RSIMDRTFED FPWQVRVEVD GVDIEVPEDA EGILVANIGS YMGGVDLWQN 600
    EDETYENFDP QSMHDKIVEV VSISGTWHLG KLQVGLSRAR RLAQGSAVKI 650
    QLCAPLPVQI DGEPWNQQPC TLTISHHGQA FMLKRAAEEP LGHAAAIITD 700
    VLENAETNQV INASQKRTLL QEMALRLT 728
    Length:728
    Mass (Da):79,984
    Last modified:March 27, 2002 - v2
    Checksum:i9E3190721C083DE9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti509 – 5091N → T in BAA09856. (PubMed:8605313)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63787 mRNA. Translation: BAA09856.1.
    AL133421 Genomic DNA. Translation: CAB62604.1.
    AB005249 Genomic DNA. Translation: BAB09956.1.
    CP002688 Genomic DNA. Translation: AED91221.1.
    BT004148 mRNA. Translation: AAO42169.1.
    PIRiS71467.
    RefSeqiNP_196409.1. NM_120874.2.
    UniGeneiAt.260.

    Genome annotation databases

    EnsemblPlantsiAT5G07920.1; AT5G07920.1; AT5G07920.
    GeneIDi830686.
    KEGGiath:AT5G07920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63787 mRNA. Translation: BAA09856.1 .
    AL133421 Genomic DNA. Translation: CAB62604.1 .
    AB005249 Genomic DNA. Translation: BAB09956.1 .
    CP002688 Genomic DNA. Translation: AED91221.1 .
    BT004148 mRNA. Translation: AAO42169.1 .
    PIRi S71467.
    RefSeqi NP_196409.1. NM_120874.2.
    UniGenei At.260.

    3D structure databases

    ProteinModelPortali Q39017.
    SMRi Q39017. Positions 78-137, 145-217.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G07920.1-P.

    Proteomic databases

    PaxDbi Q39017.
    PRIDEi Q39017.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G07920.1 ; AT5G07920.1 ; AT5G07920 .
    GeneIDi 830686.
    KEGGi ath:AT5G07920.

    Organism-specific databases

    TAIRi AT5G07920.

    Phylogenomic databases

    eggNOGi NOG47311.
    HOGENOMi HOG000005923.
    InParanoidi Q39017.
    KOi K00901.
    OMAi TISHHGQ.
    PhylomeDBi Q39017.

    Enzyme and pathway databases

    BioCyci ARA:AT5G07920-MONOMER.

    Gene expression databases

    Genevestigatori Q39017.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana."
      Katagiri T., Mizoguchi T., Shinozaki K.
      Plant Mol. Biol. 30:647-653(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Volatile C6-aldehydes and allo-ocimene activate defense genes and induce resistance against Botrytis cinerea in Arabidopsis thaliana."
      Kishimoto K., Matsui K., Ozawa R., Takabayashi J.
      Plant Cell Physiol. 46:1093-1102(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
      Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
      Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-491 AND LYS-500.
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiDGK1_ARATH
    AccessioniPrimary (citable) accession number: Q39017
    Secondary accession number(s): Q9SD92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 27, 2002
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Treatment with (E)-2-hexenal, (Z)-3-hexenal and allo-ocimene increases plant resistance against the necrotrophic fungal pathogen Botrytis cinerea.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3