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Q39017 (DGK1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol kinase 1
Short name=DAG kinase 1
EC=2.7.1.107
Alternative name(s):
Diglyceride kinase 1
Short name=DGK 1
Gene names
Name:DGK1
Ordered Locus Names:At5g07920
ORF Names:F13G24.120, MXM12.16
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length728 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Subunit structure

Monomer By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Detected in roots, shoots, and leaves.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 1 DAGKc domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 728728Diacylglycerol kinase 1
PRO_0000218475

Regions

Transmembrane27 – 4822Helical; Potential
Domain357 – 496140DAGKc
Zinc finger79 – 13759Phorbol-ester/DAG-type 1
Zinc finger149 – 21264Phorbol-ester/DAG-type 2

Amino acid modifications

Cross-link491Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.6
Cross-link500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.6

Experimental info

Sequence conflict5091N → T in BAA09856. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q39017 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: 9E3190721C083DE9

FASTA72879,984
        10         20         30         40         50         60 
MDDDGELGMF FPSWTSKNPI DTVESRGLMF SCFVAALVGI LTIAYTAFQW RRNINLSWTK 

        70         80         90        100        110        120 
AIARSKKNPK ARHKVPVAPH SWELDPIARA KNLNCCVCLK SMSPSQAIVA SESFFHRCTI 

       130        140        150        160        170        180 
CGAAAHFNCS SSAPKDCKCV SMVGFEHVVH QWAVRWTEGA DQTDDSSFCS YCDESCSSSF 

       190        200        210        220        230        240 
LGGSPIWCCL WCQRLVHVDC HSNMSNETGD ICDLGPLRRL ILCPLYVKEL TRNPSGGFLS 

       250        260        270        280        290        300 
SITHGANELA STALASIRIQ SKKYKQTNET SADTGNSGSN CDESTESTAD TGPTVNGAHA 

       310        320        330        340        350        360 
VLENSISVMN GDSSNGDSDS NGKLEKKPSV KRTGSFGQKE YHALRSKLKY ELADLPSDAR 

       370        380        390        400        410        420 
PLLVFINKKS GAQRGDSLRQ RLHLHLNPVQ VFELSSVQGP EVGLFLFRKV PHFRVLVCGG 

       430        440        450        460        470        480 
DGTAGWVLDA IEKQNFISPP AVAILPAGTG NDLSRVLNWG GGLGSVERQG GLSTVLQNIE 

       490        500        510        520        530        540 
HAAVTVLDRW KVSILNQQGK QLQPPKYMNN YIGVGCDAKV ALEIHNLREE NPERFYSQFM 

       550        560        570        580        590        600 
NKVLYAREGA RSIMDRTFED FPWQVRVEVD GVDIEVPEDA EGILVANIGS YMGGVDLWQN 

       610        620        630        640        650        660 
EDETYENFDP QSMHDKIVEV VSISGTWHLG KLQVGLSRAR RLAQGSAVKI QLCAPLPVQI 

       670        680        690        700        710        720 
DGEPWNQQPC TLTISHHGQA FMLKRAAEEP LGHAAAIITD VLENAETNQV INASQKRTLL 


QEMALRLT

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana."
Katagiri T., Mizoguchi T., Shinozaki K.
Plant Mol. Biol. 30:647-653(1996) [PubMed: 8605313] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
Mol. Cell. Proteomics 6:601-610(2007) [PubMed: 17272265] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-491 AND LYS-500, MASS SPECTROMETRY.
Strain: cv. Landsberg erecta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D63787 mRNA. Translation: BAA09856.1.
AL133421 Genomic DNA. Translation: CAB62604.1.
AB005249 Genomic DNA. Translation: BAB09956.1.
CP002688 Genomic DNA. Translation: AED91221.1.
BT004148 mRNA. Translation: AAO42169.1.
IPIIPI00527797.
PIRS71467.
RefSeqNP_196409.1. NM_120874.2.
UniGeneAt.260.

3D structure databases

ProteinModelPortalQ39017.
SMRQ39017. Positions 78-137, 145-217.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ39017.

Proteomic databases

PRIDEQ39017.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G07920.1; AT5G07920.1; AT5G07920.
GeneID830686.
GenomeReviewsGene locus AT5G07920 in contig BA000015_GR.
KEGGath:AT5G07920.
NMPDRfig|3702.1.peg.22933.

Organism-specific databases

TAIRAt5g07920.

Phylogenomic databases

eggNOGKOG1169.
GeneTreeEPGT00050000001166.
HOGENOMHBG317349.
InParanoidQ39017.
OMAVDLWQNE.
PhylomeDBQ39017.
ProtClustDBCLSN2687236.

Gene expression databases

ArrayExpressQ39017.
GenevestigatorQ39017.
GermOnlineAT5G07920. Arabidopsis thaliana.

Family and domain databases

InterProIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
Gene3DG3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
KOK00901.
PfamPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
PROSITEPS50146. DAGK. 1 hit.
PS00479. ZF_DAG_PE_1. False negative.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDGK1_ARATH
AccessionPrimary (citable) accession number: Q39017
Secondary accession number(s): Q9SD92
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 27, 2002
Last modified: November 16, 2011
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents