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Protein

Diacylglycerol kinase 1

Gene

DGK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri79 – 13759Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri149 – 21264Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: TAIR
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G07920-MONOMER.
ReactomeiREACT_329868. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 1 (EC:2.7.1.107)
Short name:
AtDGK1
Short name:
DAG kinase 1
Alternative name(s):
Diglyceride kinase 1
Short name:
DGK 1
Gene namesi
Name:DGK1
Ordered Locus Names:At5g07920
ORF Names:F13G24.120, MXM12.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G07920.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei27 – 4822HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 728728Diacylglycerol kinase 1PRO_0000218475Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki491 – 491Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki500 – 500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ39017.
PRIDEiQ39017.

Expressioni

Tissue specificityi

Expressed in roots, shoots, and leaves.1 Publication

Inductioni

By volatile C6-aldehydes ((E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenol), and allo-ocimene.1 Publication

Gene expression databases

GenevestigatoriQ39017.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G07920.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ39017.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini357 – 496140DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri79 – 13759Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri149 – 21264Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
HOGENOMiHOG000005923.
InParanoidiQ39017.
KOiK00901.
OMAiTISHHGQ.
PhylomeDBiQ39017.

Family and domain databases

InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDDGELGMF FPSWTSKNPI DTVESRGLMF SCFVAALVGI LTIAYTAFQW
60 70 80 90 100
RRNINLSWTK AIARSKKNPK ARHKVPVAPH SWELDPIARA KNLNCCVCLK
110 120 130 140 150
SMSPSQAIVA SESFFHRCTI CGAAAHFNCS SSAPKDCKCV SMVGFEHVVH
160 170 180 190 200
QWAVRWTEGA DQTDDSSFCS YCDESCSSSF LGGSPIWCCL WCQRLVHVDC
210 220 230 240 250
HSNMSNETGD ICDLGPLRRL ILCPLYVKEL TRNPSGGFLS SITHGANELA
260 270 280 290 300
STALASIRIQ SKKYKQTNET SADTGNSGSN CDESTESTAD TGPTVNGAHA
310 320 330 340 350
VLENSISVMN GDSSNGDSDS NGKLEKKPSV KRTGSFGQKE YHALRSKLKY
360 370 380 390 400
ELADLPSDAR PLLVFINKKS GAQRGDSLRQ RLHLHLNPVQ VFELSSVQGP
410 420 430 440 450
EVGLFLFRKV PHFRVLVCGG DGTAGWVLDA IEKQNFISPP AVAILPAGTG
460 470 480 490 500
NDLSRVLNWG GGLGSVERQG GLSTVLQNIE HAAVTVLDRW KVSILNQQGK
510 520 530 540 550
QLQPPKYMNN YIGVGCDAKV ALEIHNLREE NPERFYSQFM NKVLYAREGA
560 570 580 590 600
RSIMDRTFED FPWQVRVEVD GVDIEVPEDA EGILVANIGS YMGGVDLWQN
610 620 630 640 650
EDETYENFDP QSMHDKIVEV VSISGTWHLG KLQVGLSRAR RLAQGSAVKI
660 670 680 690 700
QLCAPLPVQI DGEPWNQQPC TLTISHHGQA FMLKRAAEEP LGHAAAIITD
710 720
VLENAETNQV INASQKRTLL QEMALRLT
Length:728
Mass (Da):79,984
Last modified:March 27, 2002 - v2
Checksum:i9E3190721C083DE9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti509 – 5091N → T in BAA09856 (PubMed:8605313).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63787 mRNA. Translation: BAA09856.1.
AL133421 Genomic DNA. Translation: CAB62604.1.
AB005249 Genomic DNA. Translation: BAB09956.1.
CP002688 Genomic DNA. Translation: AED91221.1.
BT004148 mRNA. Translation: AAO42169.1.
PIRiS71467.
RefSeqiNP_196409.1. NM_120874.2.
UniGeneiAt.260.

Genome annotation databases

EnsemblPlantsiAT5G07920.1; AT5G07920.1; AT5G07920.
GeneIDi830686.
KEGGiath:AT5G07920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63787 mRNA. Translation: BAA09856.1.
AL133421 Genomic DNA. Translation: CAB62604.1.
AB005249 Genomic DNA. Translation: BAB09956.1.
CP002688 Genomic DNA. Translation: AED91221.1.
BT004148 mRNA. Translation: AAO42169.1.
PIRiS71467.
RefSeqiNP_196409.1. NM_120874.2.
UniGeneiAt.260.

3D structure databases

ProteinModelPortaliQ39017.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G07920.1-P.

Proteomic databases

PaxDbiQ39017.
PRIDEiQ39017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G07920.1; AT5G07920.1; AT5G07920.
GeneIDi830686.
KEGGiath:AT5G07920.

Organism-specific databases

TAIRiAT5G07920.

Phylogenomic databases

eggNOGiNOG47311.
HOGENOMiHOG000005923.
InParanoidiQ39017.
KOiK00901.
OMAiTISHHGQ.
PhylomeDBiQ39017.

Enzyme and pathway databases

BioCyciARA:AT5G07920-MONOMER.
ReactomeiREACT_329868. Effects of PIP2 hydrolysis.

Miscellaneous databases

PROiQ39017.

Gene expression databases

GenevestigatoriQ39017.

Family and domain databases

InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana."
    Katagiri T., Mizoguchi T., Shinozaki K.
    Plant Mol. Biol. 30:647-653(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Volatile C6-aldehydes and allo-ocimene activate defense genes and induce resistance against Botrytis cinerea in Arabidopsis thaliana."
    Kishimoto K., Matsui K., Ozawa R., Takabayashi J.
    Plant Cell Physiol. 46:1093-1102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
    Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
    Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-491 AND LYS-500.
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiDGK1_ARATH
AccessioniPrimary (citable) accession number: Q39017
Secondary accession number(s): Q9SD92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 27, 2002
Last modified: April 29, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Treatment with (E)-2-hexenal, (Z)-3-hexenal and allo-ocimene increases plant resistance against the necrotrophic fungal pathogen Botrytis cinerea.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.