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Protein

Diacylglycerol kinase 1

Gene

DGK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress (By similarity).By similarity

Miscellaneous

Treatment with (E)-2-hexenal, (Z)-3-hexenal and allo-ocimene increases plant resistance against the necrotrophic fungal pathogen Botrytis cinerea.1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri79 – 137Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST59
Zinc fingeri149 – 212Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST64

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: TAIR
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPlant defense, Stress response
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G07920-MONOMER
ReactomeiR-ATH-114508 Effects of PIP2 hydrolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 1 (EC:2.7.1.107)
Short name:
AtDGK1
Short name:
DAG kinase 1
Alternative name(s):
Diglyceride kinase 1
Short name:
DGK 1
Gene namesi
Name:DGK1
Ordered Locus Names:At5g07920
ORF Names:F13G24.120, MXM12.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G07920
TAIRilocus:2142788 AT5G07920

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei27 – 48HelicalSequence analysisAdd BLAST22

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184751 – 728Diacylglycerol kinase 1Add BLAST728

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki491Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ39017
PRIDEiQ39017

PTM databases

iPTMnetiQ39017

Expressioni

Tissue specificityi

Expressed in roots, shoots, and leaves.1 Publication

Inductioni

By volatile C6-aldehydes ((E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenol), and allo-ocimene.1 Publication

Gene expression databases

ExpressionAtlasiQ39017 baseline and differential
GenevisibleiQ39017 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G07920.1

Structurei

3D structure databases

ProteinModelPortaliQ39017
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini357 – 496DAGKcPROSITE-ProRule annotationAdd BLAST140

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri79 – 137Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST59
Zinc fingeri149 – 212Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST64

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
HOGENOMiHOG000005923
InParanoidiQ39017
KOiK00901
OMAiFHRCTIC
OrthoDBiEOG09360446
PhylomeDBiQ39017

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50081 ZF_DAG_PE_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q39017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDDGELGMF FPSWTSKNPI DTVESRGLMF SCFVAALVGI LTIAYTAFQW
60 70 80 90 100
RRNINLSWTK AIARSKKNPK ARHKVPVAPH SWELDPIARA KNLNCCVCLK
110 120 130 140 150
SMSPSQAIVA SESFFHRCTI CGAAAHFNCS SSAPKDCKCV SMVGFEHVVH
160 170 180 190 200
QWAVRWTEGA DQTDDSSFCS YCDESCSSSF LGGSPIWCCL WCQRLVHVDC
210 220 230 240 250
HSNMSNETGD ICDLGPLRRL ILCPLYVKEL TRNPSGGFLS SITHGANELA
260 270 280 290 300
STALASIRIQ SKKYKQTNET SADTGNSGSN CDESTESTAD TGPTVNGAHA
310 320 330 340 350
VLENSISVMN GDSSNGDSDS NGKLEKKPSV KRTGSFGQKE YHALRSKLKY
360 370 380 390 400
ELADLPSDAR PLLVFINKKS GAQRGDSLRQ RLHLHLNPVQ VFELSSVQGP
410 420 430 440 450
EVGLFLFRKV PHFRVLVCGG DGTAGWVLDA IEKQNFISPP AVAILPAGTG
460 470 480 490 500
NDLSRVLNWG GGLGSVERQG GLSTVLQNIE HAAVTVLDRW KVSILNQQGK
510 520 530 540 550
QLQPPKYMNN YIGVGCDAKV ALEIHNLREE NPERFYSQFM NKVLYAREGA
560 570 580 590 600
RSIMDRTFED FPWQVRVEVD GVDIEVPEDA EGILVANIGS YMGGVDLWQN
610 620 630 640 650
EDETYENFDP QSMHDKIVEV VSISGTWHLG KLQVGLSRAR RLAQGSAVKI
660 670 680 690 700
QLCAPLPVQI DGEPWNQQPC TLTISHHGQA FMLKRAAEEP LGHAAAIITD
710 720
VLENAETNQV INASQKRTLL QEMALRLT
Length:728
Mass (Da):79,984
Last modified:March 27, 2002 - v2
Checksum:i9E3190721C083DE9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti509N → T in BAA09856 (PubMed:8605313).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63787 mRNA Translation: BAA09856.1
AL133421 Genomic DNA Translation: CAB62604.1
AB005249 Genomic DNA Translation: BAB09956.1
CP002688 Genomic DNA Translation: AED91221.1
CP002688 Genomic DNA Translation: ANM69404.1
BT004148 mRNA Translation: AAO42169.1
PIRiS71467
RefSeqiNP_001331085.1, NM_001342968.1
NP_196409.1, NM_120874.3
UniGeneiAt.260

Genome annotation databases

EnsemblPlantsiAT5G07920.1; AT5G07920.1; AT5G07920
AT5G07920.3; AT5G07920.3; AT5G07920
GeneIDi830686
GrameneiAT5G07920.1; AT5G07920.1; AT5G07920
AT5G07920.3; AT5G07920.3; AT5G07920
KEGGiath:AT5G07920

Similar proteinsi

Entry informationi

Entry nameiDGK1_ARATH
AccessioniPrimary (citable) accession number: Q39017
Secondary accession number(s): Q9SD92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 27, 2002
Last modified: May 23, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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