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Q39016 (CDPKB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-dependent protein kinase 11

EC=2.7.11.1
Alternative name(s):
Calcium-dependent protein kinase isoform CDPK2
Short name=AtCDPK2
Gene names
Name:CPK11
Synonyms:CDPK2
Ordered Locus Names:At1g35670
ORF Names:F15O4.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in signal transduction pathways that involve calcium as a second messenger. Functions as regulator of the calcium-mediated abscisic acid (ABA) signaling pathway. Phosphorylates ABA-responsive transcription factors ABF1 and ABF4 in vitro. Ref.1 Ref.9

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.

Subunit structure

Interacts with Di19. Ref.8

Subcellular location

Cytoplasm. Nucleus Ref.8.

Induction

By drought and high-slat stress, but not by low-temperature, heat stress or abscisic acid treatment. Ref.1

Domain

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (290-320) inactivates kinase activity under calcium-free conditions By similarity.

Disruption phenotype

Mutant cpk11-2 shows reduced ABA and salt responsiveness in seed germination. Ref.9

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.

Contains 4 EF-hand domains.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAF79386.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
   LigandATP-binding
Calcium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of abscisic acid-activated signaling pathway

Inferred from mutant phenotype Ref.9. Source: TAIR

protein autophosphorylation

Inferred from direct assay PubMed 21477822. Source: TAIR

protein phosphorylation

Inferred from direct assay Ref.1. Source: TAIR

regulation of inward rectifier potassium channel activity

Inferred from direct assay PubMed 23449501. Source: TAIR

regulation of pollen tube growth

Inferred from mutant phenotype PubMed 23449501. Source: TAIR

   Cellular_componentcytosol

Inferred from direct assay PubMed 21166475PubMed 23449501. Source: TAIR

nucleus

Inferred from direct assay PubMed 23449501. Source: TAIR

plasma membrane

Inferred from direct assay PubMed 17317660. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

calcium ion binding

Inferred from electronic annotation. Source: InterPro

calmodulin-dependent protein kinase activity

Inferred from direct assay Ref.1. Source: TAIR

protein binding

Inferred from physical interaction Ref.8PubMed 19130088. Source: IntAct

protein kinase activity

Inferred from direct assay PubMed 23449501. Source: TAIR

protein serine/threonine kinase activity

Inferred from direct assay PubMed 21477822. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495Calcium-dependent protein kinase 11
PRO_0000304513

Regions

Domain26 – 284259Protein kinase
Domain327 – 36236EF-hand 1
Domain363 – 39836EF-hand 2
Domain399 – 43436EF-hand 3
Domain435 – 46834EF-hand 4
Nucleotide binding32 – 409ATP By similarity
Calcium binding340 – 351121 By similarity
Calcium binding376 – 387122 By similarity
Calcium binding412 – 423123 By similarity
Calcium binding446 – 457124 By similarity
Region290 – 32031Autoinhibitory domain By similarity

Sites

Active site1501Proton acceptor By similarity
Binding site551ATP By similarity

Experimental info

Mutagenesis1501D → A: Loss of activity. Ref.8
Mutagenesis1711D → A: Loss of activity. Ref.8
Mutagenesis3061F → A: Constitutive activity. Ref.8
Sequence conflict4031N → I in BAA04830. Ref.1
Sequence conflict4101Y → D in BAA04830. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q39016 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 2DD0ED8C234EF2F7

FASTA49555,916
        10         20         30         40         50         60 
METKPNPRRP SNTVLPYQTP RLRDHYLLGK KLGQGQFGTT YLCTEKSTSA NYACKSIPKR 

        70         80         90        100        110        120 
KLVCREDYED VWREIQIMHH LSEHPNVVRI KGTYEDSVFV HIVMEVCEGG ELFDRIVSKG 

       130        140        150        160        170        180 
HFSEREAVKL IKTILGVVEA CHSLGVMHRD LKPENFLFDS PKDDAKLKAT DFGLSVFYKP 

       190        200        210        220        230        240 
GQYLYDVVGS PYYVAPEVLK KCYGPEIDVW SAGVILYILL SGVPPFWAET ESGIFRQILQ 

       250        260        270        280        290        300 
GKLDFKSDPW PTISEAAKDL IYKMLERSPK KRISAHEALC HPWIVDEQAA PDKPLDPAVL 

       310        320        330        340        350        360 
SRLKQFSQMN KIKKMALRVI AERLSEEEIG GLKELFKMID TDNSGTITFE ELKAGLKRVG 

       370        380        390        400        410        420 
SELMESEIKS LMDAADIDNS GTIDYGEFLA ATLHMNKMER EENLVAAFSY FDKDGSGYIT 

       430        440        450        460        470        480 
IDELQSACTE FGLCDTPLDD MIKEIDLDND GKIDFSEFTA MMRKGDGVGR SRTMMKNLNF 

       490 
NIADAFGVDG EKSDD 

« Hide

References

« Hide 'large scale' references
[1]"Two genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana."
Urao T., Katagiri T., Mizoguchi T., Yamaguchi-Shinozaki K., Hayashida N., Shinozaki K.
Mol. Gen. Genet. 244:331-340(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The CDPK superfamily of protein kinases."
Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
New Phytol. 151:175-183(2001)
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"The Arabidopsis CDPK-SnRK superfamily of protein kinases."
Hrabak E.M., Chan C.W.M., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.-K., Harmon A.C.
Plant Physiol. 132:666-680(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"A novel yeast two-hybrid approach to identify CDPK substrates: characterization of the interaction between AtCPK11 and AtDi19, a nuclear zinc finger protein."
Rodriguez Milla M.A., Uno Y., Chang I.-F., Townsend J., Maher E.A., Quilici D., Cushman J.C.
FEBS Lett. 580:904-911(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DI19, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-150; ASP-171 AND PHE-306.
[9]"Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis."
Zhu S.-Y., Yu X.-C., Wang X.-J., Zhao R., Li Y., Fan R.-C., Shang Y., Du S.-Y., Wang X.-F., Wu F.-Q., Xu Y.-H., Zhang X.-Y., Zhang D.-P.
Plant Cell 19:3019-3036(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D21806 mRNA. Translation: BAA04830.1.
AC007887 Genomic DNA. Translation: AAF79386.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31822.1.
AY050981 mRNA. Translation: AAK93658.1.
AY113986 mRNA. Translation: AAM45034.1.
PIRS46284.
RefSeqNP_174807.1. NM_103271.3.
UniGeneAt.24141.

3D structure databases

ProteinModelPortalQ39016.
SMRQ39016. Positions 22-488.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid25703. 26 interactions.
IntActQ39016. 24 interactions.
MINTMINT-1788888.

Proteomic databases

PaxDbQ39016.
PRIDEQ39016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G35670.1; AT1G35670.1; AT1G35670.
GeneID840471.
KEGGath:AT1G35670.

Organism-specific databases

TAIRAT1G35670.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233030.
InParanoidQ39016.
KOK13412.
OMATSANYAC.

Enzyme and pathway databases

BioCycARA:AT1G35670-MONOMER.

Gene expression databases

ArrayExpressQ39016.
GenevestigatorQ39016.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDPKB_ARATH
AccessionPrimary (citable) accession number: Q39016
Secondary accession number(s): Q949P0, Q9LQH7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 3, 2011
Last modified: June 11, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names