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Q39016

- CDPKB_ARATH

UniProt

Q39016 - CDPKB_ARATH

Protein

Calcium-dependent protein kinase 11

Gene

CPK11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (03 May 2011)
      Previous versions | rss
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    Functioni

    May play a role in signal transduction pathways that involve calcium as a second messenger. Functions as regulator of the calcium-mediated abscisic acid (ABA) signaling pathway. Phosphorylates ABA-responsive transcription factors ABF1 and ABF4 in vitro.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551ATPPROSITE-ProRule annotation
    Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 409ATPPROSITE-ProRule annotation
    Calcium bindingi340 – 351121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi376 – 387122PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi412 – 423123PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi446 – 457124PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. calmodulin-dependent protein kinase activity Source: TAIR
    4. protein binding Source: IntAct
    5. protein kinase activity Source: TAIR
    6. protein serine/threonine kinase activity Source: TAIR

    GO - Biological processi

    1. positive regulation of abscisic acid-activated signaling pathway Source: TAIR
    2. protein autophosphorylation Source: TAIR
    3. protein phosphorylation Source: TAIR
    4. regulation of inward rectifier potassium channel activity Source: TAIR
    5. regulation of pollen tube growth Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G35670-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-dependent protein kinase 11 (EC:2.7.11.1)
    Alternative name(s):
    Calcium-dependent protein kinase isoform CDPK2
    Short name:
    AtCDPK2
    Gene namesi
    Name:CPK11
    Synonyms:CDPK2
    Ordered Locus Names:At1g35670
    ORF Names:F15O4.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G35670.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. nucleus Source: TAIR
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mutant cpk11-2 shows reduced ABA and salt responsiveness in seed germination.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi150 – 1501D → A: Loss of activity. 1 Publication
    Mutagenesisi171 – 1711D → A: Loss of activity. 1 Publication
    Mutagenesisi306 – 3061F → A: Constitutive activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Calcium-dependent protein kinase 11PRO_0000304513Add
    BLAST

    Proteomic databases

    PaxDbiQ39016.
    PRIDEiQ39016.

    Expressioni

    Inductioni

    By drought and high-slat stress, but not by low-temperature, heat stress or abscisic acid treatment.1 Publication

    Gene expression databases

    ArrayExpressiQ39016.
    GenevestigatoriQ39016.

    Interactioni

    Subunit structurei

    Interacts with Di19.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ADF3Q9ZSK45EBI-979321,EBI-2009725
    At1g52740Q9C9449EBI-979321,EBI-1537419
    At3g26510Q27GK55EBI-979321,EBI-2297208
    At5g52550Q940C26EBI-979321,EBI-2298422
    BT2Q94BN07EBI-979321,EBI-540986
    CHLPQ9CA675EBI-979321,EBI-2298544
    DI19-1Q3908325EBI-979321,EBI-979339
    dl3455wO233386EBI-979321,EBI-2296482
    F5I14.18O044824EBI-979321,EBI-2298606
    MTACP2O808004EBI-979321,EBI-2298689
    TOC33O236804EBI-979321,EBI-639377

    Protein-protein interaction databases

    BioGridi25703. 26 interactions.
    IntActiQ39016. 24 interactions.
    MINTiMINT-1788888.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39016.
    SMRiQ39016. Positions 22-488.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 284259Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini327 – 36236EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini363 – 39836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini399 – 43436EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini435 – 46834EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni290 – 32031Autoinhibitory domainBy similarityAdd
    BLAST

    Domaini

    There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (290-320) inactivates kinase activity under calcium-free conditions By similarity.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
    Contains 4 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233030.
    InParanoidiQ39016.
    KOiK13412.
    OMAiTSANYAC.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q39016-1 [UniParc]FASTAAdd to Basket

    « Hide

    METKPNPRRP SNTVLPYQTP RLRDHYLLGK KLGQGQFGTT YLCTEKSTSA    50
    NYACKSIPKR KLVCREDYED VWREIQIMHH LSEHPNVVRI KGTYEDSVFV 100
    HIVMEVCEGG ELFDRIVSKG HFSEREAVKL IKTILGVVEA CHSLGVMHRD 150
    LKPENFLFDS PKDDAKLKAT DFGLSVFYKP GQYLYDVVGS PYYVAPEVLK 200
    KCYGPEIDVW SAGVILYILL SGVPPFWAET ESGIFRQILQ GKLDFKSDPW 250
    PTISEAAKDL IYKMLERSPK KRISAHEALC HPWIVDEQAA PDKPLDPAVL 300
    SRLKQFSQMN KIKKMALRVI AERLSEEEIG GLKELFKMID TDNSGTITFE 350
    ELKAGLKRVG SELMESEIKS LMDAADIDNS GTIDYGEFLA ATLHMNKMER 400
    EENLVAAFSY FDKDGSGYIT IDELQSACTE FGLCDTPLDD MIKEIDLDND 450
    GKIDFSEFTA MMRKGDGVGR SRTMMKNLNF NIADAFGVDG EKSDD 495
    Length:495
    Mass (Da):55,916
    Last modified:May 3, 2011 - v2
    Checksum:i2DD0ED8C234EF2F7
    GO

    Sequence cautioni

    The sequence AAF79386.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti403 – 4031N → I in BAA04830. (PubMed:8078458)Curated
    Sequence conflicti410 – 4101Y → D in BAA04830. (PubMed:8078458)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21806 mRNA. Translation: BAA04830.1.
    AC007887 Genomic DNA. Translation: AAF79386.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE31822.1.
    AY050981 mRNA. Translation: AAK93658.1.
    AY113986 mRNA. Translation: AAM45034.1.
    PIRiS46284.
    RefSeqiNP_174807.1. NM_103271.3.
    UniGeneiAt.24141.

    Genome annotation databases

    EnsemblPlantsiAT1G35670.1; AT1G35670.1; AT1G35670.
    GeneIDi840471.
    KEGGiath:AT1G35670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21806 mRNA. Translation: BAA04830.1 .
    AC007887 Genomic DNA. Translation: AAF79386.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE31822.1 .
    AY050981 mRNA. Translation: AAK93658.1 .
    AY113986 mRNA. Translation: AAM45034.1 .
    PIRi S46284.
    RefSeqi NP_174807.1. NM_103271.3.
    UniGenei At.24141.

    3D structure databases

    ProteinModelPortali Q39016.
    SMRi Q39016. Positions 22-488.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 25703. 26 interactions.
    IntActi Q39016. 24 interactions.
    MINTi MINT-1788888.

    Proteomic databases

    PaxDbi Q39016.
    PRIDEi Q39016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G35670.1 ; AT1G35670.1 ; AT1G35670 .
    GeneIDi 840471.
    KEGGi ath:AT1G35670.

    Organism-specific databases

    TAIRi AT1G35670.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233030.
    InParanoidi Q39016.
    KOi K13412.
    OMAi TSANYAC.

    Enzyme and pathway databases

    BioCyci ARA:AT1G35670-MONOMER.

    Gene expression databases

    ArrayExpressi Q39016.
    Genevestigatori Q39016.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana."
      Urao T., Katagiri T., Mizoguchi T., Yamaguchi-Shinozaki K., Hayashida N., Shinozaki K.
      Mol. Gen. Genet. 244:331-340(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "The CDPK superfamily of protein kinases."
      Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
      New Phytol. 151:175-183(2001)
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
      Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
      Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. Cited for: GENE FAMILY, NOMENCLATURE.
    8. "A novel yeast two-hybrid approach to identify CDPK substrates: characterization of the interaction between AtCPK11 and AtDi19, a nuclear zinc finger protein."
      Rodriguez Milla M.A., Uno Y., Chang I.-F., Townsend J., Maher E.A., Quilici D., Cushman J.C.
      FEBS Lett. 580:904-911(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DI19, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-150; ASP-171 AND PHE-306.
    9. "Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis."
      Zhu S.-Y., Yu X.-C., Wang X.-J., Zhao R., Li Y., Fan R.-C., Shang Y., Du S.-Y., Wang X.-F., Wu F.-Q., Xu Y.-H., Zhang X.-Y., Zhang D.-P.
      Plant Cell 19:3019-3036(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCDPKB_ARATH
    AccessioniPrimary (citable) accession number: Q39016
    Secondary accession number(s): Q949P0, Q9LQH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3