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Q39016

- CDPKB_ARATH

UniProt

Q39016 - CDPKB_ARATH

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Protein
Calcium-dependent protein kinase 11
Gene
CPK11, CDPK2, At1g35670, F15O4.8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Functions as regulator of the calcium-mediated abscisic acid (ABA) signaling pathway. Phosphorylates ABA-responsive transcription factors ABF1 and ABF4 in vitro.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATP By similarity
Active sitei150 – 1501Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 409ATP By similarity
Calcium bindingi340 – 351121 By similarity
Add
BLAST
Calcium bindingi376 – 387122 By similarity
Add
BLAST
Calcium bindingi412 – 423123 By similarity
Add
BLAST
Calcium bindingi446 – 457124 By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. calmodulin-dependent protein kinase activity Source: TAIR
  4. protein binding Source: IntAct
  5. protein kinase activity Source: TAIR
  6. protein serine/threonine kinase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. positive regulation of abscisic acid-activated signaling pathway Source: TAIR
  2. protein autophosphorylation Source: TAIR
  3. protein phosphorylation Source: TAIR
  4. regulation of inward rectifier potassium channel activity Source: TAIR
  5. regulation of pollen tube growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G35670-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 11 (EC:2.7.11.1)
Alternative name(s):
Calcium-dependent protein kinase isoform CDPK2
Short name:
AtCDPK2
Gene namesi
Name:CPK11
Synonyms:CDPK2
Ordered Locus Names:At1g35670
ORF Names:F15O4.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G35670.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. nucleus Source: TAIR
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant cpk11-2 shows reduced ABA and salt responsiveness in seed germination.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi150 – 1501D → A: Loss of activity. 1 Publication
Mutagenesisi171 – 1711D → A: Loss of activity. 1 Publication
Mutagenesisi306 – 3061F → A: Constitutive activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Calcium-dependent protein kinase 11
PRO_0000304513Add
BLAST

Proteomic databases

PaxDbiQ39016.
PRIDEiQ39016.

Expressioni

Inductioni

By drought and high-slat stress, but not by low-temperature, heat stress or abscisic acid treatment.1 Publication

Gene expression databases

ArrayExpressiQ39016.
GenevestigatoriQ39016.

Interactioni

Subunit structurei

Interacts with Di19.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ADF3Q9ZSK45EBI-979321,EBI-2009725
At1g52740Q9C9449EBI-979321,EBI-1537419
At3g26510Q27GK55EBI-979321,EBI-2297208
At5g52550Q940C26EBI-979321,EBI-2298422
BT2Q94BN07EBI-979321,EBI-540986
CHLPQ9CA675EBI-979321,EBI-2298544
DI19-1Q3908325EBI-979321,EBI-979339
dl3455wO233386EBI-979321,EBI-2296482
F5I14.18O044824EBI-979321,EBI-2298606
MTACP2O808004EBI-979321,EBI-2298689
TOC33O236804EBI-979321,EBI-639377

Protein-protein interaction databases

BioGridi25703. 26 interactions.
IntActiQ39016. 24 interactions.
MINTiMINT-1788888.

Structurei

3D structure databases

ProteinModelPortaliQ39016.
SMRiQ39016. Positions 22-488.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 284259Protein kinase
Add
BLAST
Domaini327 – 36236EF-hand 1
Add
BLAST
Domaini363 – 39836EF-hand 2
Add
BLAST
Domaini399 – 43436EF-hand 3
Add
BLAST
Domaini435 – 46834EF-hand 4
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni290 – 32031Autoinhibitory domain By similarity
Add
BLAST

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (290-320) inactivates kinase activity under calcium-free conditions By similarity.

Sequence similaritiesi

Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233030.
InParanoidiQ39016.
KOiK13412.
OMAiTSANYAC.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39016-1 [UniParc]FASTAAdd to Basket

« Hide

METKPNPRRP SNTVLPYQTP RLRDHYLLGK KLGQGQFGTT YLCTEKSTSA    50
NYACKSIPKR KLVCREDYED VWREIQIMHH LSEHPNVVRI KGTYEDSVFV 100
HIVMEVCEGG ELFDRIVSKG HFSEREAVKL IKTILGVVEA CHSLGVMHRD 150
LKPENFLFDS PKDDAKLKAT DFGLSVFYKP GQYLYDVVGS PYYVAPEVLK 200
KCYGPEIDVW SAGVILYILL SGVPPFWAET ESGIFRQILQ GKLDFKSDPW 250
PTISEAAKDL IYKMLERSPK KRISAHEALC HPWIVDEQAA PDKPLDPAVL 300
SRLKQFSQMN KIKKMALRVI AERLSEEEIG GLKELFKMID TDNSGTITFE 350
ELKAGLKRVG SELMESEIKS LMDAADIDNS GTIDYGEFLA ATLHMNKMER 400
EENLVAAFSY FDKDGSGYIT IDELQSACTE FGLCDTPLDD MIKEIDLDND 450
GKIDFSEFTA MMRKGDGVGR SRTMMKNLNF NIADAFGVDG EKSDD 495
Length:495
Mass (Da):55,916
Last modified:May 3, 2011 - v2
Checksum:i2DD0ED8C234EF2F7
GO

Sequence cautioni

The sequence AAF79386.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti403 – 4031N → I in BAA04830. 1 Publication
Sequence conflicti410 – 4101Y → D in BAA04830. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D21806 mRNA. Translation: BAA04830.1.
AC007887 Genomic DNA. Translation: AAF79386.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31822.1.
AY050981 mRNA. Translation: AAK93658.1.
AY113986 mRNA. Translation: AAM45034.1.
PIRiS46284.
RefSeqiNP_174807.1. NM_103271.3.
UniGeneiAt.24141.

Genome annotation databases

EnsemblPlantsiAT1G35670.1; AT1G35670.1; AT1G35670.
GeneIDi840471.
KEGGiath:AT1G35670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D21806 mRNA. Translation: BAA04830.1 .
AC007887 Genomic DNA. Translation: AAF79386.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE31822.1 .
AY050981 mRNA. Translation: AAK93658.1 .
AY113986 mRNA. Translation: AAM45034.1 .
PIRi S46284.
RefSeqi NP_174807.1. NM_103271.3.
UniGenei At.24141.

3D structure databases

ProteinModelPortali Q39016.
SMRi Q39016. Positions 22-488.
ModBasei Search...

Protein-protein interaction databases

BioGridi 25703. 26 interactions.
IntActi Q39016. 24 interactions.
MINTi MINT-1788888.

Proteomic databases

PaxDbi Q39016.
PRIDEi Q39016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G35670.1 ; AT1G35670.1 ; AT1G35670 .
GeneIDi 840471.
KEGGi ath:AT1G35670.

Organism-specific databases

TAIRi AT1G35670.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233030.
InParanoidi Q39016.
KOi K13412.
OMAi TSANYAC.

Enzyme and pathway databases

BioCyci ARA:AT1G35670-MONOMER.

Gene expression databases

ArrayExpressi Q39016.
Genevestigatori Q39016.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana."
    Urao T., Katagiri T., Mizoguchi T., Yamaguchi-Shinozaki K., Hayashida N., Shinozaki K.
    Mol. Gen. Genet. 244:331-340(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The CDPK superfamily of protein kinases."
    Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
    New Phytol. 151:175-183(2001)
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
    Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
    Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. Cited for: GENE FAMILY, NOMENCLATURE.
  8. "A novel yeast two-hybrid approach to identify CDPK substrates: characterization of the interaction between AtCPK11 and AtDi19, a nuclear zinc finger protein."
    Rodriguez Milla M.A., Uno Y., Chang I.-F., Townsend J., Maher E.A., Quilici D., Cushman J.C.
    FEBS Lett. 580:904-911(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DI19, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-150; ASP-171 AND PHE-306.
  9. "Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis."
    Zhu S.-Y., Yu X.-C., Wang X.-J., Zhao R., Li Y., Fan R.-C., Shang Y., Du S.-Y., Wang X.-F., Wu F.-Q., Xu Y.-H., Zhang X.-Y., Zhang D.-P.
    Plant Cell 19:3019-3036(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCDPKB_ARATH
AccessioniPrimary (citable) accession number: Q39016
Secondary accession number(s): Q949P0, Q9LQH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 3, 2011
Last modified: June 11, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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