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Protein

Shaggy-related protein kinase eta

Gene

ASK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negative regulator in brassinosteroid signal transduction pathway important for plant growth. May be also involved in auxin signaling pathway. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates BHLH150, beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues. Upon brassinosteroid signaling, inhibits stomatal development by phosphorylating and inhibiting the MAPKK kinase YDA and the MAPK kinases MKK4 and MKK5 (PubMed:11847343, PubMed:12114546, PubMed:12427989, PubMed:17280695, PubMed:22307275, PubMed:23341468). Phosphorylates BSK1, BSK3, BSK5, BSK6, BSK8 AND BSK11 in vitro (PubMed:23496207).7 Publications

Miscellaneous

Unlike other GSK3 kinases, does not require a priming phosphorylation event or the presence of a scaffold protein to phosphorylate its substrates.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Enzyme regulationi

Inactivated by an unknown mechanism after binding of brassinosteroids to the brassinosteroid receptor complex (Probable). Inhibited by lithium. Inhibited by dephosphorylation at Tyr-200 by BSU1 (PubMed:21855796). Competitive inhibition by KIB1 that reduces substrate (e.g. BZR1) access (PubMed:28575660).Curated2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei165Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: TAIR
  • detection of brassinosteroid stimulus Source: TAIR
  • leaf morphogenesis Source: TAIR
  • multidimensional cell growth Source: TAIR
  • negative regulation of brassinosteroid mediated signaling pathway Source: UniProtKB
  • positive regulation of protein export from nucleus Source: TAIR
  • protein autophosphorylation Source: TAIR
  • protein phosphorylation Source: TAIR
  • response to auxin Source: TAIR
  • root hair cell differentiation Source: TAIR
  • viral process Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processBrassinosteroid signaling pathway, Host-virus interaction
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.26 399
ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response

Names & Taxonomyi

Protein namesi
Recommended name:
Shaggy-related protein kinase eta1 Publication (EC:2.7.11.12 Publications)
Alternative name(s):
ASK-eta1 Publication
Protein BRASSINOSTEROID INSENSITIVE 21 Publication
Protein ULTRACURVATA 11 Publication
Shaggy-related protein kinase 211 Publication
Short name:
AtSK211 Publication
Gene namesi
Name:ASK71 Publication
Synonyms:BIN21 Publication, DWF121 Publication, SK211 Publication, UCU11 Publication
Ordered Locus Names:At4g18710Imported
ORF Names:F28A21.120Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G18710
TAIRilocus:2124082 AT4G18710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69K → R: Abolishes kinase activity. 2 Publications1
Mutagenesisi80R → A: No effect. 1 Publication1
Mutagenesisi200Y → F: Normal interaction with KIB1. 1 Publication1
Mutagenesisi261T → I in bin2-2; brassinosteroid-insensitive dwarf mutant; increased kinase activity. 1 Publication1
Mutagenesisi263E → K in dwf12-2D/bin2-1; brassinosteroid-insensitive dwarf mutant; increased kinase activity. Reduced interaction with KIB1. 3 Publications1
Mutagenesisi264E → K in dwf12-1D/ucu1-1/ucu1-2; brassinosteroid-insensitive dwarf mutant. 2 Publications1
Mutagenesisi284P → S in ucu1-3; leaves rolled spirally downward. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862221 – 380Shaggy-related protein kinase etaAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104PhosphothreonineSequence analysis1
Modified residuei105PhosphoserineSequence analysis1
Modified residuei187PhosphoserineSequence analysis1
Modified residuei200Phosphotyrosine1 Publication1
Modified residuei220PhosphothreonineSequence analysis1
Modified residuei261PhosphothreonineSequence analysis1
Modified residuei310PhosphoserineSequence analysis1
Modified residuei314PhosphothreonineSequence analysis1
Modified residuei353PhosphoserineSequence analysis1

Post-translational modificationi

Autophosphorylated mainly on threonine and serine residues.1 Publication
Ubiquitination and subsequent proteasomal degradation mediated by KIB1.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ39011

PTM databases

iPTMnetiQ39011

Expressioni

Tissue specificityi

In the two outer cell layers of the developing seed coat and restricted to the suspensor cells in developing embryos.1 Publication

Gene expression databases

ExpressionAtlasiQ39011 baseline and differential
GenevisibleiQ39011 AT

Interactioni

Subunit structurei

Interacts in vitro with the C-terminal fragment of BZR1 and with BES1/BZR2, but not through the kinase domain. Interacts with BHLH150, beet curly top virus AL4/C4 and tomato golden mosaic virus AL4/AC4. Interacts with YDA. Interacts with MKK4. Interacts with KIB1 and KIB2 in a brassinosteroid (BR)-dependent manner (PubMed:28575660). Interacts with BSK1, BSK6, BSK8 and BSK11 (PubMed:23496207).8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi12898, 30 interactors
DIPiDIP-46010N
IntActiQ39011, 10 interactors
MINTiQ39011
STRINGi3702.AT4G18710.1

Structurei

3D structure databases

ProteinModelPortaliQ39011
SMRiQ39011
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 324Protein kinasePROSITE-ProRule annotationAdd BLAST285

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0658 Eukaryota
COG0515 LUCA
HOGENOMiHOG000233017
InParanoidiQ39011
KOiK14502
OMAiHARRHCG
OrthoDBiEOG09360BBG
PhylomeDBiQ39011

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q39011-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADDKEMPAA VVDGHDQVTG HIISTTIGGK NGEPKQTISY MAERVVGTGS
60 70 80 90 100
FGIVFQAKCL ETGETVAIKK VLQDRRYKNR ELQLMRVMDH PNVVCLKHCF
110 120 130 140 150
FSTTSKDELF LNLVMEYVPE SLYRVLKHYS SANQRMPLVY VKLYMYQIFR
160 170 180 190 200
GLAYIHNVAG VCHRDLKPQN LLVDPLTHQV KICDFGSAKQ LVKGEANISY
210 220 230 240 250
ICSRFYRAPE LIFGATEYTT SIDIWSAGCV LAELLLGQPL FPGENAVDQL
260 270 280 290 300
VEIIKVLGTP TREEIRCMNP HYTDFRFPQI KAHPWHKIFH KRMPPEAIDF
310 320 330 340 350
ASRLLQYSPS LRCTALEACA HPFFDELREP NARLPNGRPF PPLFNFKQEV
360 370 380
AGSSPELVNK LIPDHIKRQL GLSFLNQSGT
Length:380
Mass (Da):43,099
Last modified:May 10, 2004 - v2
Checksum:i86C2FD5F77201168
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105S → E in CAA64409 (Ref. 1) Curated1
Sequence conflicti105S → E in CAA70144 (PubMed:9611268).Curated1
Sequence conflicti121S → T in CAA64409 (Ref. 1) Curated1
Sequence conflicti121S → T in CAA70144 (PubMed:9611268).Curated1
Sequence conflicti145M → S in CAA64409 (Ref. 1) Curated1
Sequence conflicti145M → S in CAA70144 (PubMed:9611268).Curated1
Sequence conflicti157 – 159NVA → SCP in CAA64409 (Ref. 1) Curated3
Sequence conflicti157 – 159NVA → SCP in CAA70144 (PubMed:9611268).Curated3
Sequence conflicti196A → P in CAA64409 (Ref. 1) Curated1
Sequence conflicti196A → P in CAA70144 (PubMed:9611268).Curated1
Sequence conflicti271H → N in CAA64409 (Ref. 1) Curated1
Sequence conflicti271H → N in CAA70144 (PubMed:9611268).Curated1
Sequence conflicti276R → K in CAA64409 (Ref. 1) Curated1
Sequence conflicti276R → K in CAA70144 (PubMed:9611268).Curated1
Sequence conflicti298 – 300IDF → VDL in CAA64409 (Ref. 1) Curated3
Sequence conflicti298 – 300IDF → VDL in CAA70144 (PubMed:9611268).Curated3
Sequence conflicti327L → H in AAL77705 (PubMed:14593172).Curated1
Sequence conflicti340F → L in CAA64409 (Ref. 1) Curated1
Sequence conflicti340F → L in CAA70144 (PubMed:9611268).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94939 mRNA Translation: CAA64409.1
Y08947 Genomic DNA Translation: CAA70144.1
AY157149 Genomic DNA Translation: AAN71719.1
AL035526 Genomic DNA Translation: CAB37456.1
AL161549 Genomic DNA Translation: CAB78873.1
CP002687 Genomic DNA Translation: AEE84079.1
AY075699 mRNA Translation: AAL77705.1
BT026031 mRNA Translation: ABG48387.1
AY086529 mRNA Translation: AAM63594.1
PIRiT04863
RefSeqiNP_193606.1, NM_117987.4 [Q39011-1]
UniGeneiAt.22875

Genome annotation databases

EnsemblPlantsiAT4G18710.1; AT4G18710.1; AT4G18710 [Q39011-1]
GeneIDi827605
GrameneiAT4G18710.1; AT4G18710.1; AT4G18710 [Q39011-1]
KEGGiath:AT4G18710

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKSG7_ARATH
AccessioniPrimary (citable) accession number: Q39011
Secondary accession number(s): Q147R2, Q8S9H8, Q9SN42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 10, 2004
Last modified: May 23, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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