Reviewed, UniProtKB/Swiss-Prot Q39008 (M3K1_ARATH)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase 1
      Short name=MAP kinase kinase kinase 1
      Short name=AtMEKK1
      Short name=ARAKIN
    EC=2.7.11.25

Gene names

Name:	MEKK1
Ordered Locus Names:	At4g08500
ORF Names:	T15F16.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MEK1/MKK2 and MPK4/MPK6). Activates downstream MKK2, MKK4 and MKK5. Ref.7 Ref.9
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with MEK1, MMK2 and MPK4. May form a ternary complex with MEK1 or MMK2 and MPK4. Ref.6
Induction	By touch, cold and salinity stress. Ref.1 Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAA99196.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAA99196.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	MAPKKK cascade Inferred from mutant phenotype. Source: TAIR protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro response to cold Ref.1 Inferred from expression pattern. Source: TAIR response to salt stress Ref.5 Inferred from expression pattern. Source: TAIR response to wounding Inferred from direct assay. Source: TAIR
Cellular component	nucleus Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from direct assay. Source: TAIR MAP kinase kinase kinase activity Inferred from direct assay. Source: TAIR kinase binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
WRKY53	Q9SUP6	3	EBI-994439,EBI-1235980

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 608	608	Mitogen-activated protein kinase kinase kinase 1		PRO_0000245826
Regions
	Domain	333 – 587	255	Protein kinase
	Nucleotide binding	339 – 347	9	ATP By similarity
	Region	1 – 325	325	Regulatory region
	Region	192 – 234	43	Binding with MPK4
Sites
	Active site	456	1	Proton acceptor By similarity
	Binding site	361	1	ATP By similarity
Experimental info
	Sequence conflict	39	1	D → A in AAC28196. Ref.1
	Sequence conflict	341	1	R → L in AAL32537. Ref.3
	Sequence conflict	341	1	R → L in AAN15435. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q39008-1 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: 70C1A1C314E2DFDD	FASTA	608	66,024
	10 20 30 40 50 60 MDRILARMKK STGRRGGDKN ITPVRRLERR DAARNINYDA ASCSSSSAED LSVSTSSLMT 70 80 90 100 110 120 RSLEFPEPTS FRIGGGVGEM DRIYRSLGVS GPDDLAISFD AWEACKKRSS SDVVNRFKSF 130 140 150 160 170 180 DLDKVRDQDL SEEGPSGVVV GSDSMNHKVQ GQDLSEAGPS GGIVTELSEI GNLITPVDRL 190 200 210 220 230 240 VADGVVENRR VMERTPTIVK SKGYLVPNNV VAVGVGVGGG IKGLRPPVLK PPPAMKRPPI 250 260 270 280 290 300 DHRGSSWDFL THFAPSETVK RPSSSSSSSE DGCDEEEGKE EEAEAEEMGA RFIQLGDTAD 310 320 330 340 350 360 ETCSFTTNEG DSSSTVSNTS PIYPDGGAII TSWQKGQLLG RGSFGSVYEG ISGDGDFFAV 370 380 390 400 410 420 KEVSLLDQGS QAQECIQQLE GEIKLLSQLQ HQNIVRYRGT AKDGSNLYIF LELVTQGSLL 430 440 450 460 470 480 KLYQRYQLRD SVVSLYTRQI LDGLKYLHDK GFIHRDIKCA NILVDANGAV KLADFGLAKV 490 500 510 520 530 540 SKFNDIKSCK GTPFWMAPEV INRKDSDGYG SPADIWSLGC TVLEMCTGQI PYSDLEPVQA 550 560 570 580 590 600 LFRIGRGTLP EVPDTLSLDA RLFILKCLKV NPEERPTAAE LLNHPFVRRP LPSVGSGGSG SASPLLRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana." Mizoguchi T., Irie K., Hirayama T., Hayashida N., Yamaguchi-Shinozaki K., Matsumoto K., Shinozaki K. Proc. Natl. Acad. Sci. U.S.A. 93:765-769(1996) [PubMed: 8570631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis thaliana cDNA isolated by functional complementation shows homology to serine/threonine protein kinases." Covic L., Lew R.R. Biochim. Biophys. Acta 1305:125-129(1996) [PubMed: 8597596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 115-608.
[5]	"Functional characterization of ARAKIN (ATMEKK1): a possible mediator in an osmotic stress response pathway in higher plants." Covic L., Silva N.F., Lew R.R. Biochim. Biophys. Acta 1451:242-254(1999) [PubMed: 10556579] [Abstract] Cited for: INDUCTION.
[6]	"Isolation of ATMEKK1 (a MAP kinase kinase kinase)-interacting proteins and analysis of a MAP kinase cascade in Arabidopsis." Ichimura K., Mizoguchi T., Irie K., Morris P.C., Giraudat J., Matsumoto K., Shinozaki K. Biochem. Biophys. Res. Commun. 253:532-543(1998) [PubMed: 9878570] [Abstract] Cited for: SUBUNIT, INTERACTION WITH MEK1, MMK2 AND MPK4.
[7]	"MAP kinase signalling cascade in Arabidopsis innate immunity." Asai T., Tena G., Plotnikova J., Willmann M.R., Chiu W.-L., Gomez-Gomez L., Boller T., Ausubel F.M., Sheen J. Nature 415:977-983(2002) [PubMed: 11875555] [Abstract] Cited for: FUNCTION.
[8]	"Mitogen-activated protein kinase cascades in plants: a new nomenclature." MAPK group Trends Plant Sci. 7:301-308(2002) [PubMed: 12119167] [Abstract] Cited for: NOMENCLATURE.
[9]	"The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis." Teige M., Scheikl E., Eulgem T., Doczi R., Ichimura K., Shinozaki K., Dangl J.L., Hirt H. Mol. Cell 15:141-152(2004) [PubMed: 15225555] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50468 mRNA. Translation: BAA09057.1. AF076275 Genomic DNA. Translation: AAC28196.1. AL161511 Genomic DNA. Translation: CAB77975.1. AY062459 mRNA. Translation: AAL32537.1. BT000116 mRNA. Translation: AAN15435.1. L43125 Genomic DNA. Translation: AAA99196.1. Sequence problems.
IPI	IPI00521547.
PIR	T01833.
RefSeq	NP_192590.1.
UniGene	At.21066
3D structure databases
SMR	Q39008. Positions 332-588.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39008. 3 interactions.
STRING	Q39008.
Proteomic databases
PRIDE	Q39008.
Genome annotation databases
GeneID	826409.
GenomeReviews	Gene locus AT4G08500 in contig CT486007_GR.
KEGG	ath:AT4G08500.
NMPDR	fig|3702.1.peg.18504.
Organism-specific databases
GeneFarm	859. 89.
TAIR	At4g08500.
Phylogenomic databases
eggNOG	KOG0198.
HOGENOM	HBG593886.
InParanoid	Q39008.
OMA	KEDSKLY.
PhylomeDB	Q39008.
Enzyme and pathway databases
BRENDA	2.7.11.25. 302.
Gene expression databases
Genevestigator	Q39008.
GermOnline	AT4G08500. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M3K1_ARATH

Accession

Primary (citable) accession number: Q39008
Secondary accession number(s): O81470, Q39020, Q8W4N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	July 11, 2006
Last modified:	February 9, 2010
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)