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Protein

Mitogen-activated protein kinase kinase kinase 1

Gene

MEKK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). Activates by phosphorylation the downstream MKK2, MKK4 and MKK5 in a calcium-dependent manner.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cold via CRLK1-mediated phosphorylation and leading to elevated kinase activity towards MKK2.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei361 – 3611ATPPROSITE-ProRule annotation
Active sitei456 – 4561Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi339 – 3479ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: TAIR
  • kinase binding Source: TAIR
  • MAP kinase kinase kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • cold acclimation Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • MAPK cascade Source: TAIR
  • protein autophosphorylation Source: TAIR
  • response to cold Source: UniProtKB
  • response to L-glutamate Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to salt stress Source: TAIR
  • response to wounding Source: TAIR
  • root meristem growth Source: TAIR
  • root system development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Immunity, Innate immunity, Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G08500-MONOMER.
BRENDAi2.7.11.25. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 1 (EC:2.7.11.25)
Short name:
ARAKIN
Short name:
AtMEKK1
Short name:
MAP kinase kinase kinase 1
Gene namesi
Name:MEKK1
Ordered Locus Names:At4g08500
ORF Names:T15F16.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G08500.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: UniProtKB
  • nucleus Source: TAIR
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Mitogen-activated protein kinase kinase kinase 1PRO_0000245826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei603 – 6031PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by CRLK1 in response to cold.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39008.
PRIDEiQ39008.

PTM databases

iPTMnetiQ39008.

Expressioni

Inductioni

By touch, cold and salinity stress.2 Publications

Gene expression databases

GenevisibleiQ39008. AT.

Interactioni

Subunit structurei

Interacts with MKK1, MMK2 and MPK4. May form a ternary complex composed of MEKK1 and MKK1/MKK2 and MPK4 (PubMed:18982020, PubMed:9878570). Interacts with RACK1A, RACK1B and RACK1C (PubMed:25731164). Binds to CRLK1 (PubMed:20724845).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MKK1Q94A064EBI-994439,EBI-994464
WRKY53Q9SUP65EBI-994439,EBI-1235980

GO - Molecular functioni

  • kinase binding Source: TAIR

Protein-protein interaction databases

BioGridi11709. 8 interactions.
IntActiQ39008. 3 interactions.
STRINGi3702.AT4G08500.1.

Structurei

3D structure databases

ProteinModelPortaliQ39008.
SMRiQ39008. Positions 309-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini333 – 587255Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 325325Regulatory region1 PublicationAdd
BLAST
Regioni192 – 23443Binding with MPK41 PublicationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000239933.
InParanoidiQ39008.
KOiK13414.
OMAiNKPCLER.
OrthoDBiEOG0936083X.
PhylomeDBiQ39008.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39008-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRILARMKK STGRRGGDKN ITPVRRLERR DAARNINYDA ASCSSSSAED
60 70 80 90 100
LSVSTSSLMT RSLEFPEPTS FRIGGGVGEM DRIYRSLGVS GPDDLAISFD
110 120 130 140 150
AWEACKKRSS SDVVNRFKSF DLDKVRDQDL SEEGPSGVVV GSDSMNHKVQ
160 170 180 190 200
GQDLSEAGPS GGIVTELSEI GNLITPVDRL VADGVVENRR VMERTPTIVK
210 220 230 240 250
SKGYLVPNNV VAVGVGVGGG IKGLRPPVLK PPPAMKRPPI DHRGSSWDFL
260 270 280 290 300
THFAPSETVK RPSSSSSSSE DGCDEEEGKE EEAEAEEMGA RFIQLGDTAD
310 320 330 340 350
ETCSFTTNEG DSSSTVSNTS PIYPDGGAII TSWQKGQLLG RGSFGSVYEG
360 370 380 390 400
ISGDGDFFAV KEVSLLDQGS QAQECIQQLE GEIKLLSQLQ HQNIVRYRGT
410 420 430 440 450
AKDGSNLYIF LELVTQGSLL KLYQRYQLRD SVVSLYTRQI LDGLKYLHDK
460 470 480 490 500
GFIHRDIKCA NILVDANGAV KLADFGLAKV SKFNDIKSCK GTPFWMAPEV
510 520 530 540 550
INRKDSDGYG SPADIWSLGC TVLEMCTGQI PYSDLEPVQA LFRIGRGTLP
560 570 580 590 600
EVPDTLSLDA RLFILKCLKV NPEERPTAAE LLNHPFVRRP LPSVGSGGSG

SASPLLRR
Length:608
Mass (Da):66,024
Last modified:July 11, 2006 - v2
Checksum:i70C1A1C314E2DFDD
GO

Sequence cautioni

The sequence AAA99196 differs from that shown.Sequencing errors.Curated
The sequence AAA99196 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391D → A in AAC28196 (PubMed:8570631).Curated
Sequence conflicti341 – 3411R → L in AAL32537 (PubMed:14593172).Curated
Sequence conflicti341 – 3411R → L in AAN15435 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50468 mRNA. Translation: BAA09057.1.
AF076275 Genomic DNA. Translation: AAC28196.1.
AL161511 Genomic DNA. Translation: CAB77975.1.
CP002687 Genomic DNA. Translation: AEE82651.1.
AY062459 mRNA. Translation: AAL32537.1.
BT000116 mRNA. Translation: AAN15435.1.
L43125 Genomic DNA. Translation: AAA99196.1. Sequence problems.
PIRiT01833.
RefSeqiNP_192590.1. NM_116919.3.
UniGeneiAt.21066.

Genome annotation databases

EnsemblPlantsiAT4G08500.1; AT4G08500.1; AT4G08500.
GeneIDi826409.
GrameneiAT4G08500.1; AT4G08500.1; AT4G08500.
KEGGiath:AT4G08500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50468 mRNA. Translation: BAA09057.1.
AF076275 Genomic DNA. Translation: AAC28196.1.
AL161511 Genomic DNA. Translation: CAB77975.1.
CP002687 Genomic DNA. Translation: AEE82651.1.
AY062459 mRNA. Translation: AAL32537.1.
BT000116 mRNA. Translation: AAN15435.1.
L43125 Genomic DNA. Translation: AAA99196.1. Sequence problems.
PIRiT01833.
RefSeqiNP_192590.1. NM_116919.3.
UniGeneiAt.21066.

3D structure databases

ProteinModelPortaliQ39008.
SMRiQ39008. Positions 309-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11709. 8 interactions.
IntActiQ39008. 3 interactions.
STRINGi3702.AT4G08500.1.

PTM databases

iPTMnetiQ39008.

Proteomic databases

PaxDbiQ39008.
PRIDEiQ39008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G08500.1; AT4G08500.1; AT4G08500.
GeneIDi826409.
GrameneiAT4G08500.1; AT4G08500.1; AT4G08500.
KEGGiath:AT4G08500.

Organism-specific databases

TAIRiAT4G08500.

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000239933.
InParanoidiQ39008.
KOiK13414.
OMAiNKPCLER.
OrthoDBiEOG0936083X.
PhylomeDBiQ39008.

Enzyme and pathway databases

BioCyciARA:AT4G08500-MONOMER.
BRENDAi2.7.11.25. 399.

Miscellaneous databases

PROiQ39008.

Gene expression databases

GenevisibleiQ39008. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM3K1_ARATH
AccessioniPrimary (citable) accession number: Q39008
Secondary accession number(s): O81470, Q39020, Q8W4N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: September 7, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.