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Q38ZM8 (DDL_LACSS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LCA_0049
OrganismLactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]
Taxonomic identifier314315 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030456

Regions

Domain146 – 341196ATP-grasp
Nucleotide binding172 – 22554ATP By similarity

Sites

Metal binding2951Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 2 By similarity
Metal binding3101Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q38ZM8 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 71EEB914E0DDEB8A

FASTA35038,477
        10         20         30         40         50         60 
MKIVVLAGGR STERNVSLTS GHKITHALQT KGHDVAFVDL FLGNELKDGA TIDSLYSSDP 

        70         80         90        100        110        120 
VEKDYDIDDE VLTDDDINKL REDGSTQLFG PNVLEICKTA DIVFLALHGG DGENGKVQAV 

       130        140        150        160        170        180 
LDVFEIPYTG SDTLAAGITM SKKVSKEILL FNNIPTARFV AAYRHQPMPE MPFEYPVVVK 

       190        200        210        220        230        240 
PSNGGSSVGT HIVHNEAELK PAVEDALRFD NEALIEEFIK GREFSLGVIN EQPLPAIEIE 

       250        260        270        280        290        300 
VNDGWYDFEH KFVTGNTTKF VTPPNNLPDD VHEAMKQMAL DAMHALGLTN YARIDFFWSP 

       310        320        330        340        350 
ETGLYVIEGN TLPGMTPLSL IPQEAEVLGI SYPDLCEMIV NGKLALLNEK 

« Hide

References

[1]"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K."
Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M.
Nat. Biotechnol. 23:1527-1533(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 23K.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936503 Genomic DNA. Translation: CAI54349.1.
RefSeqYP_394661.1. NC_007576.1.

3D structure databases

ProteinModelPortalQ38ZM8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314315.LSA0049.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI54349; CAI54349; LCA_0049.
GeneID3778554.
KEGGlsa:LSA0049.
PATRIC22272868. VBILacSak116432_0048.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LACSS
AccessionPrimary (citable) accession number: Q38ZM8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways